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The map label for this gene is mrdA [H]

Identifier: 45657144

GI number: 45657144

Start: 1557030

End: 1558973

Strand: Direct

Name: mrdA [H]

Synonym: LIC11261

Alternate gene names: 45657144

Gene position: 1557030-1558973 (Clockwise)

Preceding gene: 45657143

Following gene: 45657145

Centisome position: 36.4

GC content: 39.71

Gene sequence:

>1944_bases
TTGTTAGGTGGAGCTCAGTCTGCGTCGGAATACCGACTTGAAAAAAGTTTTAGACTTCGTCTTTATACTTTTTCCGGACT
TGTAGTATTTGCTCTTGCCGCCTTTATATTTCAGTTATTCAATCTTCAGATTTTAAACGGAACGGATAACGCCCTTAAGG
CCGAAAAGTTTGTTCGAAAAAGTGAAATTATGCCTGCGGCTAGGGGACAGATGTTTGATCGAAATTTTCTCACACCTGAA
ACTTCTATGGCACTCGTGTCGAACTACTCTTCTTTGGACGTAGTGTTAAATACTTCTTTATTTAAATATGATCCAACTTC
TGTAAAGGCGTTCATTCAAGAATTTGCAAGAACTCTTTCTATACCAATTTCTTATTACCAGGATGAGTTGATCGAACCCC
GCTTTTCCAGAAACTTAAAGGCTAAAAAGCCGATCGTACTTTTAGAAGGAATCAGCACTGCACAACAAGAAAGAATATCA
GTATTTGATAATATATCTAAATATATTGTTTTACTTCCTTCCCCGAGAAGAGTTTACAAAATGGGTCCAGCCCTTGCACA
CGTGACCGGTTATATAGGAAAACCTACTAGGGATGATTTGGTTTCTGGAAAAATTAAATCGTATCAGTATTTGGGAAAAG
ACGGACTGGAGTTAGAATATGATTCGGAACTCAGAGGGGTAGACGGATTTAGAATTCAAAAAAGAAGTTCCGAAGGAAAC
ATAGAAGAGGAACGGGTGGTAGAACATTCTTCTCCTGGAAATAATCTGGTTCTAACGATAGACAAGGATATACAAATCGC
CGCTTATAAAGCTTTAAAGGGAAGTAGAGGAACCGCGATCGCTATCAAAGTTGCTACCGGAGAAATTTTGGCGATGGTTT
CTAATCCGAGTTATGACCCAAATATACTTTCAGGAAAAAATAGAGCAGATCGAACTGCACATTTTAAAAGAGTCAAAGAC
AACGGAGGATTTTTAAATCTAGCGATTAAATCTAAATTTCCACCCGCTTCTACTTATAAAACGTTAGTTGCTCTTGCCGC
GTTGGAAAGCCAACACAAGATAGGATATACCCCAGAAACTACGTTTTCTTGTAACGGAAGTTTTGTATTGAAATCAACTT
TTGCAGGGGTTCCAGATCAGGTATTTTTATGTTGGGAAAAGGGAGGGCATGGAACGAATGATCTTGCGCATGCTTTGCAA
AAATCTTGTTCTGTATATTTTTACAACTTAGGATATAAATTAGGATCAGATGCAATTTTAAATTATTCCAGACTTTTTGG
ATTGGAAAACAAATCCAAGGTGGATCTTCCGGATGAAACCGCCGGTTTTGTACCTTCTTCTGCATGGAAAAAAAGAACTT
ATGGAACAAAGTGGTTTGATGGAGATACGATCAATCTTTCTATCGGACAAGGATTTATTTCTACCACTCCGATAGGCATG
GCTCTTTTTTATATGGGATTGCTCAACCGAGGACAAATCTATCAACCGTATGTGGTAAGCGAAATTCGAGATCCAGTAGA
TAATTCTATTATCAATAGAACCACACCGCAGATTTTAAGAGACATACCGATCAATCAATCTTCTGTAGAAGCGATTAAAG
AAGGATTAAAACTTGTGGTAAAAAGTGGAACGGCTGCACACGTATTGAACAAACCGTTTTTACCAGAAATAGCGGGAAAA
ACCGGAACCGCTCAGACTAGGAGAAGAGGGGCTTCTGGTTCCAATCACGCATGGTTTGTAGGATACGCTCCTGCAAACGC
TCCAGCCAGCGAACAAGTGTTAGTTGTTGTGTTTGTGGAATACGGGATCGGAGGAGCTGCTGGAGCTGCACCTGTTGCAA
GAGAAATGTTTCATGCGGCTTTTCCACCGGGAACATTCAAAAAGACGCAAGAAGTAAATCCTCAACCTAAGATTGGACAG
GAGGGAACTCTTGAAGCCAGATAA

Upstream 100 bases:

>100_bases
TCCTTATTTTTCACTTCTTTGTTTAATGCGCTCATTGCGCCCGCTTTTTTTTATGCTCTGACTTGGATTTACCAATTGGA
TCATATGGGGGACGCGTAAG

Downstream 100 bases:

>100_bases
GTCCATAGAAAAGATAGATTACTTTTTAGTAATTTCGGTAGTAATTGTCGTACTTTGTAGCGTAACTACTTTATATAGCC
AGGAAGTGAATTTTGAAGAT

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MLGGAQSASEYRLEKSFRLRLYTFSGLVVFALAAFIFQLFNLQILNGTDNALKAEKFVRKSEIMPAARGQMFDRNFLTPE
TSMALVSNYSSLDVVLNTSLFKYDPTSVKAFIQEFARTLSIPISYYQDELIEPRFSRNLKAKKPIVLLEGISTAQQERIS
VFDNISKYIVLLPSPRRVYKMGPALAHVTGYIGKPTRDDLVSGKIKSYQYLGKDGLELEYDSELRGVDGFRIQKRSSEGN
IEEERVVEHSSPGNNLVLTIDKDIQIAAYKALKGSRGTAIAIKVATGEILAMVSNPSYDPNILSGKNRADRTAHFKRVKD
NGGFLNLAIKSKFPPASTYKTLVALAALESQHKIGYTPETTFSCNGSFVLKSTFAGVPDQVFLCWEKGGHGTNDLAHALQ
KSCSVYFYNLGYKLGSDAILNYSRLFGLENKSKVDLPDETAGFVPSSAWKKRTYGTKWFDGDTINLSIGQGFISTTPIGM
ALFYMGLLNRGQIYQPYVVSEIRDPVDNSIINRTTPQILRDIPINQSSVEAIKEGLKLVVKSGTAAHVLNKPFLPEIAGK
TGTAQTRRRGASGSNHAWFVGYAPANAPASEQVLVVVFVEYGIGGAAGAAPVAREMFHAAFPPGTFKKTQEVNPQPKIGQ
EGTLEAR

Sequences:

>Translated_647_residues
MLGGAQSASEYRLEKSFRLRLYTFSGLVVFALAAFIFQLFNLQILNGTDNALKAEKFVRKSEIMPAARGQMFDRNFLTPE
TSMALVSNYSSLDVVLNTSLFKYDPTSVKAFIQEFARTLSIPISYYQDELIEPRFSRNLKAKKPIVLLEGISTAQQERIS
VFDNISKYIVLLPSPRRVYKMGPALAHVTGYIGKPTRDDLVSGKIKSYQYLGKDGLELEYDSELRGVDGFRIQKRSSEGN
IEEERVVEHSSPGNNLVLTIDKDIQIAAYKALKGSRGTAIAIKVATGEILAMVSNPSYDPNILSGKNRADRTAHFKRVKD
NGGFLNLAIKSKFPPASTYKTLVALAALESQHKIGYTPETTFSCNGSFVLKSTFAGVPDQVFLCWEKGGHGTNDLAHALQ
KSCSVYFYNLGYKLGSDAILNYSRLFGLENKSKVDLPDETAGFVPSSAWKKRTYGTKWFDGDTINLSIGQGFISTTPIGM
ALFYMGLLNRGQIYQPYVVSEIRDPVDNSIINRTTPQILRDIPINQSSVEAIKEGLKLVVKSGTAAHVLNKPFLPEIAGK
TGTAQTRRRGASGSNHAWFVGYAPANAPASEQVLVVVFVEYGIGGAAGAAPVAREMFHAAFPPGTFKKTQEVNPQPKIGQ
EGTLEAR
>Mature_647_residues
MLGGAQSASEYRLEKSFRLRLYTFSGLVVFALAAFIFQLFNLQILNGTDNALKAEKFVRKSEIMPAARGQMFDRNFLTPE
TSMALVSNYSSLDVVLNTSLFKYDPTSVKAFIQEFARTLSIPISYYQDELIEPRFSRNLKAKKPIVLLEGISTAQQERIS
VFDNISKYIVLLPSPRRVYKMGPALAHVTGYIGKPTRDDLVSGKIKSYQYLGKDGLELEYDSELRGVDGFRIQKRSSEGN
IEEERVVEHSSPGNNLVLTIDKDIQIAAYKALKGSRGTAIAIKVATGEILAMVSNPSYDPNILSGKNRADRTAHFKRVKD
NGGFLNLAIKSKFPPASTYKTLVALAALESQHKIGYTPETTFSCNGSFVLKSTFAGVPDQVFLCWEKGGHGTNDLAHALQ
KSCSVYFYNLGYKLGSDAILNYSRLFGLENKSKVDLPDETAGFVPSSAWKKRTYGTKWFDGDTINLSIGQGFISTTPIGM
ALFYMGLLNRGQIYQPYVVSEIRDPVDNSIINRTTPQILRDIPINQSSVEAIKEGLKLVVKSGTAAHVLNKPFLPEIAGK
TGTAQTRRRGASGSNHAWFVGYAPANAPASEQVLVVVFVEYGIGGAAGAAPVAREMFHAAFPPGTFKKTQEVNPQPKIGQ
EGTLEAR

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=642, Percent_Identity=27.7258566978193, Blast_Score=190, Evalue=3e-49,
Organism=Escherichia coli, GI1786272, Length=625, Percent_Identity=24.8, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 71109; Mature: 71109

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGGAQSASEYRLEKSFRLRLYTFSGLVVFALAAFIFQLFNLQILNGTDNALKAEKFVRK
CCCCCCCHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHH
SEIMPAARGQMFDRNFLTPETSMALVSNYSSLDVVLNTSLFKYDPTSVKAFIQEFARTLS
HHCCCHHHCCCCCCCCCCCCHHHHHHHCCCCEEEEEECEEEEECCHHHHHHHHHHHHHHC
IPISYYQDELIEPRFSRNLKAKKPIVLLEGISTAQQERISVFDNISKYIVLLPSPRRVYK
CCHHHHHHHHCCCHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHHHH
MGPALAHVTGYIGKPTRDDLVSGKIKSYQYLGKDGLELEYDSELRGVDGFRIQKRSSEGN
CCCHHHHHHHCCCCCCHHHHHCCCHHHHHCCCCCCCEEEECCCCCCCCCEEEEECCCCCC
IEEERVVEHSSPGNNLVLTIDKDIQIAAYKALKGSRGTAIAIKVATGEILAMVSNPSYDP
CCHHHHHHCCCCCCEEEEEECCCCEEEEEHHHCCCCCCEEEEEEECCEEEEEECCCCCCC
NILSGKNRADRTAHFKRVKDNGGFLNLAIKSKFPPASTYKTLVALAALESQHKIGYTPET
CCCCCCCCCCHHHHHHEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
TFSCNGSFVLKSTFAGVPDQVFLCWEKGGHGTNDLAHALQKSCSVYFYNLGYKLGSDAIL
EEECCCCEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCCCCHHHH
NYSRLFGLENKSKVDLPDETAGFVPSSAWKKRTYGTKWFDGDTINLSIGQGFISTTPIGM
HHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEECCCEEEEEECCCCCCCCHHHH
ALFYMGLLNRGQIYQPYVVSEIRDPVDNSIINRTTPQILRDIPINQSSVEAIKEGLKLVV
HHHHHHHCCCCCEECCHHHHHHHCCHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHEEC
KSGTAAHVLNKPFLPEIAGKTGTAQTRRRGASGSNHAWFVGYAPANAPASEQVLVVVFVE
CCCCHHHHHCCCCCHHHCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEE
YGIGGAAGAAPVAREMFHAAFPPGTFKKTQEVNPQPKIGQEGTLEAR
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLGGAQSASEYRLEKSFRLRLYTFSGLVVFALAAFIFQLFNLQILNGTDNALKAEKFVRK
CCCCCCCHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHH
SEIMPAARGQMFDRNFLTPETSMALVSNYSSLDVVLNTSLFKYDPTSVKAFIQEFARTLS
HHCCCHHHCCCCCCCCCCCCHHHHHHHCCCCEEEEEECEEEEECCHHHHHHHHHHHHHHC
IPISYYQDELIEPRFSRNLKAKKPIVLLEGISTAQQERISVFDNISKYIVLLPSPRRVYK
CCHHHHHHHHCCCHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHHHH
MGPALAHVTGYIGKPTRDDLVSGKIKSYQYLGKDGLELEYDSELRGVDGFRIQKRSSEGN
CCCHHHHHHHCCCCCCHHHHHCCCHHHHHCCCCCCCEEEECCCCCCCCCEEEEECCCCCC
IEEERVVEHSSPGNNLVLTIDKDIQIAAYKALKGSRGTAIAIKVATGEILAMVSNPSYDP
CCHHHHHHCCCCCCEEEEEECCCCEEEEEHHHCCCCCCEEEEEEECCEEEEEECCCCCCC
NILSGKNRADRTAHFKRVKDNGGFLNLAIKSKFPPASTYKTLVALAALESQHKIGYTPET
CCCCCCCCCCHHHHHHEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
TFSCNGSFVLKSTFAGVPDQVFLCWEKGGHGTNDLAHALQKSCSVYFYNLGYKLGSDAIL
EEECCCCEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCCCCHHHH
NYSRLFGLENKSKVDLPDETAGFVPSSAWKKRTYGTKWFDGDTINLSIGQGFISTTPIGM
HHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEECCCEEEEEECCCCCCCCHHHH
ALFYMGLLNRGQIYQPYVVSEIRDPVDNSIINRTTPQILRDIPINQSSVEAIKEGLKLVV
HHHHHHHCCCCCEECCHHHHHHHCCHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHEEC
KSGTAAHVLNKPFLPEIAGKTGTAQTRRRGASGSNHAWFVGYAPANAPASEQVLVVVFVE
CCCCHHHHHCCCCCHHHCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEE
YGIGGAAGAAPVAREMFHAAFPPGTFKKTQEVNPQPKIGQEGTLEAR
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]