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The map label for this gene is purB

Identifier: 45656902

GI number: 45656902

Start: 1239567

End: 1240868

Strand: Direct

Name: purB

Synonym: LIC11016

Alternate gene names: 45656902

Gene position: 1239567-1240868 (Clockwise)

Preceding gene: 45656901

Following gene: 45656903

Centisome position: 28.98

GC content: 40.86

Gene sequence:

>1302_bases
ATGATCGATCGATATTCCAATCCTGAAATTTCCAAAATCTGGGAATTAGAAAACAAGTTCGAAATTTGGAAAGAAATAGA
AATACTTGCCTGCGAAATCAGAATGAAACGAGGCGAAGTTCCACAAGAAGATTTTCAAGAAATCAAATCGAAAGCTAAGT
TTAATGTGGATGAAATCCTTGAAATTGAAAGCAAGGTTCATCACGACGTGATCGCGTTTTTGACAAATATGAATTCCTAC
ATCGGGCCTGCGGGAAGGCACGTTCATTACGGTTTGACTTCTTCGGATATTGGGGACACGGCTCTTTGTGTGCAAATGGT
TCAGGCAATGGATTTGATTCTAAAAAAAACGGACTCGCTGATCGAAGTGGTTCGGGAAAAGGCGATCCAGTATAAGAATC
TTCCCTGTATCGGACGTTCTCATGGGATCCACGCCGAACCTATGACTCTGGGACTTAAGTTCGCATTATTTTTTGAAGAA
TTAAACCGAAATAGAAAAAGAATGGCCGAAGCAAGAAACGAAATTGCTGTTGGAAAACTTTCCGGAGCCGTCGGAACTTA
TTCTAACATCGATCCTGAAATCGAAACCTACGTATGTGAGAAGATGGGTTTGAGGGTGGATCCAATTGCAACTCAGGTTG
TTTCTAGGGATCGTCATGCTTTTTATCTTTCTGTATTGGGAGTTACCGCTTCTTCTTTGGATCGTATGGCGACCGAGATC
CGACTTTTACAAAAGACAGAAGGAAGAGAAGTAGAGGAGCCTTTTTCTGCGGGTCAAAAAGGGTCATCCGCAATGCCCCA
CAAAAGAAATCCTGTGATCTGCGAAAGAATCTCCGGGCTTTCCAGAGTGATTCGCGCAAATGTAAACGTGGGTTTACAAG
ACGTGGCTCTTTGGCACGAAAGGGATATTTCTCATTCTTCTGCGGAAAGGGTTGTACTTCCAGATTCTACTATTGCTTTA
GAATATATCTTGGATAAGATGTTGTTCGTGATCAAAAATCTTCACGTTTATCCAGATGCGATCGAAAGAACGTTAGGCGT
CACTCGTGGACTTGTATTTTCGCAAAAGGTTTTACTCGCTCTGATCGAAAAAGGTAAAATTATAAGAGAAGAAGCGTATT
TGATCGTTCAAGAACACGCTATGGCGGTTTGGGCCAGCCAGTCAGAAACTTTGAAATCTAGATTGGAAAAAGACGATCGG
GTCAGTAAAGTTTTGACTCAAAAAGACTTAGACGACATTTTTAAAATAGAACCTTATTTGGAAAAAGTAGGATTGATCTA
TAAAAGATTGGGTCTTGAATAA

Upstream 100 bases:

>100_bases
AAACAAATTTCAATTCCTTTTTCTACTGATCTTGTTTTTCTTTTTACAGAATACACTTCTGTGAAAGAATGGATAGGAAT
TCAAAAAGGAACTTACAGAA

Downstream 100 bases:

>100_bases
ATGAAAATTGGAATTTTCGGTTTAGGATATACCGGAGTTCGGATTGCGGATCTTTTAAAATCTCAAACGGGGATAGAACT
AAATACATTTTCGGCTAACA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MIDRYSNPEISKIWELENKFEIWKEIEILACEIRMKRGEVPQEDFQEIKSKAKFNVDEILEIESKVHHDVIAFLTNMNSY
IGPAGRHVHYGLTSSDIGDTALCVQMVQAMDLILKKTDSLIEVVREKAIQYKNLPCIGRSHGIHAEPMTLGLKFALFFEE
LNRNRKRMAEARNEIAVGKLSGAVGTYSNIDPEIETYVCEKMGLRVDPIATQVVSRDRHAFYLSVLGVTASSLDRMATEI
RLLQKTEGREVEEPFSAGQKGSSAMPHKRNPVICERISGLSRVIRANVNVGLQDVALWHERDISHSSAERVVLPDSTIAL
EYILDKMLFVIKNLHVYPDAIERTLGVTRGLVFSQKVLLALIEKGKIIREEAYLIVQEHAMAVWASQSETLKSRLEKDDR
VSKVLTQKDLDDIFKIEPYLEKVGLIYKRLGLE

Sequences:

>Translated_433_residues
MIDRYSNPEISKIWELENKFEIWKEIEILACEIRMKRGEVPQEDFQEIKSKAKFNVDEILEIESKVHHDVIAFLTNMNSY
IGPAGRHVHYGLTSSDIGDTALCVQMVQAMDLILKKTDSLIEVVREKAIQYKNLPCIGRSHGIHAEPMTLGLKFALFFEE
LNRNRKRMAEARNEIAVGKLSGAVGTYSNIDPEIETYVCEKMGLRVDPIATQVVSRDRHAFYLSVLGVTASSLDRMATEI
RLLQKTEGREVEEPFSAGQKGSSAMPHKRNPVICERISGLSRVIRANVNVGLQDVALWHERDISHSSAERVVLPDSTIAL
EYILDKMLFVIKNLHVYPDAIERTLGVTRGLVFSQKVLLALIEKGKIIREEAYLIVQEHAMAVWASQSETLKSRLEKDDR
VSKVLTQKDLDDIFKIEPYLEKVGLIYKRLGLE
>Mature_433_residues
MIDRYSNPEISKIWELENKFEIWKEIEILACEIRMKRGEVPQEDFQEIKSKAKFNVDEILEIESKVHHDVIAFLTNMNSY
IGPAGRHVHYGLTSSDIGDTALCVQMVQAMDLILKKTDSLIEVVREKAIQYKNLPCIGRSHGIHAEPMTLGLKFALFFEE
LNRNRKRMAEARNEIAVGKLSGAVGTYSNIDPEIETYVCEKMGLRVDPIATQVVSRDRHAFYLSVLGVTASSLDRMATEI
RLLQKTEGREVEEPFSAGQKGSSAMPHKRNPVICERISGLSRVIRANVNVGLQDVALWHERDISHSSAERVVLPDSTIAL
EYILDKMLFVIKNLHVYPDAIERTLGVTRGLVFSQKVLLALIEKGKIIREEAYLIVQEHAMAVWASQSETLKSRLEKDDR
VSKVLTQKDLDDIFKIEPYLEKVGLIYKRLGLE

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI183227688, Length=400, Percent_Identity=28, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4557269, Length=428, Percent_Identity=27.803738317757, Blast_Score=155, Evalue=7e-38,
Organism=Escherichia coli, GI1787376, Length=281, Percent_Identity=27.0462633451957, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17508577, Length=399, Percent_Identity=27.8195488721804, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI32564234, Length=345, Percent_Identity=29.2753623188406, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6323391, Length=379, Percent_Identity=30.0791556728232, Blast_Score=170, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24647570, Length=431, Percent_Identity=25.0580046403712, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49203; Mature: 49203

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDRYSNPEISKIWELENKFEIWKEIEILACEIRMKRGEVPQEDFQEIKSKAKFNVDEIL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHH
EIESKVHHDVIAFLTNMNSYIGPAGRHVHYGLTSSDIGDTALCVQMVQAMDLILKKTDSL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IEVVREKAIQYKNLPCIGRSHGIHAEPMTLGLKFALFFEELNRNRKRMAEARNEIAVGKL
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEHHHH
SGAVGTYSNIDPEIETYVCEKMGLRVDPIATQVVSRDRHAFYLSVLGVTASSLDRMATEI
CCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHH
RLLQKTEGREVEEPFSAGQKGSSAMPHKRNPVICERISGLSRVIRANVNVGLQDVALWHE
HHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RDISHSSAERVVLPDSTIALEYILDKMLFVIKNLHVYPDAIERTLGVTRGLVFSQKVLLA
HCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LIEKGKIIREEAYLIVQEHAMAVWASQSETLKSRLEKDDRVSKVLTQKDLDDIFKIEPYL
HHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
EKVGLIYKRLGLE
HHHHHHHHHHCCC
>Mature Secondary Structure
MIDRYSNPEISKIWELENKFEIWKEIEILACEIRMKRGEVPQEDFQEIKSKAKFNVDEIL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHH
EIESKVHHDVIAFLTNMNSYIGPAGRHVHYGLTSSDIGDTALCVQMVQAMDLILKKTDSL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IEVVREKAIQYKNLPCIGRSHGIHAEPMTLGLKFALFFEELNRNRKRMAEARNEIAVGKL
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEHHHH
SGAVGTYSNIDPEIETYVCEKMGLRVDPIATQVVSRDRHAFYLSVLGVTASSLDRMATEI
CCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHH
RLLQKTEGREVEEPFSAGQKGSSAMPHKRNPVICERISGLSRVIRANVNVGLQDVALWHE
HHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RDISHSSAERVVLPDSTIALEYILDKMLFVIKNLHVYPDAIERTLGVTRGLVFSQKVLLA
HCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LIEKGKIIREEAYLIVQEHAMAVWASQSETLKSRLEKDDRVSKVLTQKDLDDIFKIEPYL
HHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
EKVGLIYKRLGLE
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]