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The map label for this gene is ispB

Identifier: 45656805

GI number: 45656805

Start: 1099592

End: 1100551

Strand: Direct

Name: ispB

Synonym: LIC10916

Alternate gene names: 45656805

Gene position: 1099592-1100551 (Clockwise)

Preceding gene: 45656804

Following gene: 45656807

Centisome position: 25.71

GC content: 36.25

Gene sequence:

>960_bases
GTGAAAGTTTCGGTACTGAAAGATTCTCTGATTCGGAAATTTGACAAAAAGTTAGAAACGATCATTCGGGAAGACCTTAA
AATTCTCTCCGAAATCAAAACCTATACGATTCAATCGGGTGGGAAGAGGATTCGTCCTATTCTTCATTATTGTCTCTGCC
AACTTTTAGGTTATTCCGGTAAACACTGGTTAGACGTCGGAGCCATTGCGGAGTTGATTCATGCAGCCAGTTTACTTCAT
GATGACGTGGTGGATGAGGCGGAAACTAGAAGAGGACTTCAAAGTGTGGGTTCCAAATTTGGTAATAAAACGGCTATTCT
TGCGGGAGATTATCTTCTTGCTTGTGGAATCGACCATTTAAATGGCTTGGGTTATCCTGAATTGATGGAAGTTTTTACTC
AGGTTATCAAGGATCTCTCCGTTTCTGAACTCATTCAAATGGAATGGGAAAAAAATCCTAAAATAACTTTAGATATTTAT
AATAAAATTATATATGGAAAAACTGCTTCTTTATTTGCCGTTGTGTGTGTGTCGGCGGGAATTCTTTCTGATAAAAATGA
AAAAGAAAAAAAGAAACTCAGGCAATTTGGGATTGATTTAGGTTCTTTTTTTCAGAAAAAAGACGACGCGATTGATTATT
TTACCTCTGCAAGTAAAAGTGGGAAAATTCCCCTCAAAGATTTTTATAACGGTCTTTATACGCATCCTATTTTGTTGTTA
CTTGATCGAGCTGATAGGAACGATAAAAAATTAATTCATTCTCTTTTTGCTAAGTCGGAAAGGTCGCAAGGAGACGGTAT
TGTGATTTTAAGTCTTCTCTCTCGTTATCAGATTCGAGAACGGATGGATGCGGAGTTTCAAACGATAGCGGATAATTTGC
TGAAATTCTTAAATGGTTTTTCGGAATCTACAATTCGGAATCTTTTAAAAGAACAAATTCTAAAACTTTTAGATGAATAA

Upstream 100 bases:

>100_bases
CATTCTTTCTAGACAACTTCTAATTCTGGAAAACAAACTGAAAGCTTGAAGAGAGTTCTTGCTTGAGCCTGTACCGTTGT
TTTGAATACTTGTCACCACG

Downstream 100 bases:

>100_bases
GTTTTAATTTATAATTCGTTTTTTAAAATGTAGGAACCCATACAAAATTTAAAAATGAAAGCCTCTAACATTTTATTATA
AATTTATAAATGTAAGAACT

Product: octaprenyl diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKVSVLKDSLIRKFDKKLETIIREDLKILSEIKTYTIQSGGKRIRPILHYCLCQLLGYSGKHWLDVGAIAELIHAASLLH
DDVVDEAETRRGLQSVGSKFGNKTAILAGDYLLACGIDHLNGLGYPELMEVFTQVIKDLSVSELIQMEWEKNPKITLDIY
NKIIYGKTASLFAVVCVSAGILSDKNEKEKKKLRQFGIDLGSFFQKKDDAIDYFTSASKSGKIPLKDFYNGLYTHPILLL
LDRADRNDKKLIHSLFAKSERSQGDGIVILSLLSRYQIRERMDAEFQTIADNLLKFLNGFSESTIRNLLKEQILKLLDE

Sequences:

>Translated_319_residues
MKVSVLKDSLIRKFDKKLETIIREDLKILSEIKTYTIQSGGKRIRPILHYCLCQLLGYSGKHWLDVGAIAELIHAASLLH
DDVVDEAETRRGLQSVGSKFGNKTAILAGDYLLACGIDHLNGLGYPELMEVFTQVIKDLSVSELIQMEWEKNPKITLDIY
NKIIYGKTASLFAVVCVSAGILSDKNEKEKKKLRQFGIDLGSFFQKKDDAIDYFTSASKSGKIPLKDFYNGLYTHPILLL
LDRADRNDKKLIHSLFAKSERSQGDGIVILSLLSRYQIRERMDAEFQTIADNLLKFLNGFSESTIRNLLKEQILKLLDE
>Mature_319_residues
MKVSVLKDSLIRKFDKKLETIIREDLKILSEIKTYTIQSGGKRIRPILHYCLCQLLGYSGKHWLDVGAIAELIHAASLLH
DDVVDEAETRRGLQSVGSKFGNKTAILAGDYLLACGIDHLNGLGYPELMEVFTQVIKDLSVSELIQMEWEKNPKITLDIY
NKIIYGKTASLFAVVCVSAGILSDKNEKEKKKLRQFGIDLGSFFQKKDDAIDYFTSASKSGKIPLKDFYNGLYTHPILLL
LDRADRNDKKLIHSLFAKSERSQGDGIVILSLLSRYQIRERMDAEFQTIADNLLKFLNGFSESTIRNLLKEQILKLLDE

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=229, Percent_Identity=33.1877729257642, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI4758430, Length=263, Percent_Identity=25.4752851711027, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI83700220, Length=263, Percent_Identity=25.4752851711027, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1789578, Length=245, Percent_Identity=31.8367346938775, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1786623, Length=267, Percent_Identity=29.5880149812734, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=247, Percent_Identity=33.6032388663968, Blast_Score=134, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6319475, Length=221, Percent_Identity=31.6742081447964, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24651612, Length=223, Percent_Identity=28.2511210762332, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI281365769, Length=270, Percent_Identity=25.5555555555556, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24660002, Length=270, Percent_Identity=25.5555555555556, Blast_Score=78, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36134; Mature: 36134

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSVLKDSLIRKFDKKLETIIREDLKILSEIKTYTIQSGGKRIRPILHYCLCQLLGYSG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
KHWLDVGAIAELIHAASLLHDDVVDEAETRRGLQSVGSKFGNKTAILAGDYLLACGIDHL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHCCHHH
NGLGYPELMEVFTQVIKDLSVSELIQMEWEKNPKITLDIYNKIIYGKTASLFAVVCVSAG
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHH
ILSDKNEKEKKKLRQFGIDLGSFFQKKDDAIDYFTSASKSGKIPLKDFYNGLYTHPILLL
HCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
LDRADRNDKKLIHSLFAKSERSQGDGIVILSLLSRYQIRERMDAEFQTIADNLLKFLNGF
HCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SESTIRNLLKEQILKLLDE
CHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVSVLKDSLIRKFDKKLETIIREDLKILSEIKTYTIQSGGKRIRPILHYCLCQLLGYSG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
KHWLDVGAIAELIHAASLLHDDVVDEAETRRGLQSVGSKFGNKTAILAGDYLLACGIDHL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHCCHHH
NGLGYPELMEVFTQVIKDLSVSELIQMEWEKNPKITLDIYNKIIYGKTASLFAVVCVSAG
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHH
ILSDKNEKEKKKLRQFGIDLGSFFQKKDDAIDYFTSASKSGKIPLKDFYNGLYTHPILLL
HCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
LDRADRNDKKLIHSLFAKSERSQGDGIVILSLLSRYQIRERMDAEFQTIADNLLKFLNGF
HCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SESTIRNLLKEQILKLLDE
CHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]