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The map label for this gene is ttgG [H]

Identifier: 45656390

GI number: 45656390

Start: 572671

End: 574164

Strand: Reverse

Name: ttgG [H]

Synonym: LIC10492

Alternate gene names: 45656390

Gene position: 574164-572671 (Counterclockwise)

Preceding gene: 45656391

Following gene: 45656389

Centisome position: 13.42

GC content: 34.61

Gene sequence:

>1494_bases
TTGATCATTAAATTTTTAAAATCTAAATTTGGTAAATTCTTTCTGTTATCTTTAGCAGCATATTTTATCATTTCTCTTTT
TTTAACTCGCCTCGCAAAAAATTCTCAAAGTAAAATGTTAAAAAATTCGGTTTCGATGTTAACGAAACCTCTTTCCTTTT
CACTTTCCTCTCCTAATCAATCCGAAGAAACATTTTCATCAGAAGAAGAATCACAAAATCTAATGTCCATTTTGGCGATG
AATGTAGTTAAAGAATCTATTACACCTACAGTAGAAGCCTCAGGGATGATAGACTTTATAGAAAAAGTAGACGTCTATTC
TAAAGTATCCGGAAGAATCGAAAAGGTCTATTTTAAAGAAGGGGAGGAAATTTCTCAAAATCAAAGATTATTTAAAATGG
AAACCTTACCGTTAGAACTTGAATTGCTCAAACAAGAATCTACTCTAGAAAGTTCCAAGTCACAGGTAAAACTAGCCAAA
GAAAAATATCTGAAAGCAAAAAACAACGTACTTGCTAAAATTCAGGAATACGAAAAGTCGCTTTCCGTTTTAGAAAAATC
AAAACAAGAATATGAAAAGGCTAAAAACTCTTTTGCCGGTATAGAAGAAATCTATAACGCAGGGGGATTTACTAAGGAAG
AATTCGAAAACGCTAAACTAAATCTAAATTCCAAAGAGACCTCCTATCAAATCGCGGAAAAGGACGTAGAAATCAGAAGT
ATCGGTTTAACAGATAAAGACATATTAAGAAATAATTATAAACTTCCTCAAACAAAAGAGGAACGAACCACAATACTCGA
AGAGATCAATAGCCTAATAGAAAAAGCGGAATGGGAAGTAGCAGAAGGTGTTTATAAATCTCATGAAGCACAAGTAAATT
CCACAAAAATGATGCTCAAAGAATCTTTAGTATTTTCACCTCTTACAGGAGTAATCTCAAAACAATTCAAAACCGAAGGA
GAAATTTTAAGCGGCTCTGGACCAGGACAACACGTTGTATCCGTAATTAACGTCAAACAAGTTTATGCCGTTCTTAACAT
ACCGGAATCAGAATCTATCAACTTAAAAAAAGGAATGAAAGTTTCCTTTTTAGCGGACGTTTATAAAGGTCAACAGTTTC
AAGGTATGGTTCAATTATTAAGTCCTATAGTGGATCAAAAAAGTCACACTGTAGAAATCAAAGCCTTAGTAGATAATAAA
AACAAACTACTAAAACCAGGAATGTTTATTAGAGCGAATATTACCACTGGTCAAGAACAACCTACAATACGAATTCCAAG
CAACGCAGTTGTGTTAAACGAAGACGGAACCACAGGAATTGTTTGTGTAGTCCGCAATGGAAAGAGTTATAGAAACGAAG
TCAAGTTAGGATCTCAGAGTGAAGGTAAGATCGTAATTTCGGAAGGTCTAACGGAAGGTGACGTTGTCCTTCTCGAAAGA
ATATCACAACTCAGGGACGGTATGTCCGTGAACCCCTACTTCGACAAACGATGA

Upstream 100 bases:

>100_bases
ATAATCACCAAACAGTGATTTTATGCAAAAATTTTATCCAGCAAATTATTATTTTTGAATTTTTAGAAGCCCCTCATTTG
AATCTCTGGAGGATAAAAAA

Downstream 100 bases:

>100_bases
AAAGTGTAGTTCGTTTTTGTGTTTCAAAACCGATTACGGTTTTGATGGCTTGGTTTGCGATCATAATTTTTGGAATCATT
GGATTAAAAAATGCTAAAAT

Product: heavy metal efflux pump

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MIIKFLKSKFGKFFLLSLAAYFIISLFLTRLAKNSQSKMLKNSVSMLTKPLSFSLSSPNQSEETFSSEEESQNLMSILAM
NVVKESITPTVEASGMIDFIEKVDVYSKVSGRIEKVYFKEGEEISQNQRLFKMETLPLELELLKQESTLESSKSQVKLAK
EKYLKAKNNVLAKIQEYEKSLSVLEKSKQEYEKAKNSFAGIEEIYNAGGFTKEEFENAKLNLNSKETSYQIAEKDVEIRS
IGLTDKDILRNNYKLPQTKEERTTILEEINSLIEKAEWEVAEGVYKSHEAQVNSTKMMLKESLVFSPLTGVISKQFKTEG
EILSGSGPGQHVVSVINVKQVYAVLNIPESESINLKKGMKVSFLADVYKGQQFQGMVQLLSPIVDQKSHTVEIKALVDNK
NKLLKPGMFIRANITTGQEQPTIRIPSNAVVLNEDGTTGIVCVVRNGKSYRNEVKLGSQSEGKIVISEGLTEGDVVLLER
ISQLRDGMSVNPYFDKR

Sequences:

>Translated_497_residues
MIIKFLKSKFGKFFLLSLAAYFIISLFLTRLAKNSQSKMLKNSVSMLTKPLSFSLSSPNQSEETFSSEEESQNLMSILAM
NVVKESITPTVEASGMIDFIEKVDVYSKVSGRIEKVYFKEGEEISQNQRLFKMETLPLELELLKQESTLESSKSQVKLAK
EKYLKAKNNVLAKIQEYEKSLSVLEKSKQEYEKAKNSFAGIEEIYNAGGFTKEEFENAKLNLNSKETSYQIAEKDVEIRS
IGLTDKDILRNNYKLPQTKEERTTILEEINSLIEKAEWEVAEGVYKSHEAQVNSTKMMLKESLVFSPLTGVISKQFKTEG
EILSGSGPGQHVVSVINVKQVYAVLNIPESESINLKKGMKVSFLADVYKGQQFQGMVQLLSPIVDQKSHTVEIKALVDNK
NKLLKPGMFIRANITTGQEQPTIRIPSNAVVLNEDGTTGIVCVVRNGKSYRNEVKLGSQSEGKIVISEGLTEGDVVLLER
ISQLRDGMSVNPYFDKR
>Mature_497_residues
MIIKFLKSKFGKFFLLSLAAYFIISLFLTRLAKNSQSKMLKNSVSMLTKPLSFSLSSPNQSEETFSSEEESQNLMSILAM
NVVKESITPTVEASGMIDFIEKVDVYSKVSGRIEKVYFKEGEEISQNQRLFKMETLPLELELLKQESTLESSKSQVKLAK
EKYLKAKNNVLAKIQEYEKSLSVLEKSKQEYEKAKNSFAGIEEIYNAGGFTKEEFENAKLNLNSKETSYQIAEKDVEIRS
IGLTDKDILRNNYKLPQTKEERTTILEEINSLIEKAEWEVAEGVYKSHEAQVNSTKMMLKESLVFSPLTGVISKQFKTEG
EILSGSGPGQHVVSVINVKQVYAVLNIPESESINLKKGMKVSFLADVYKGQQFQGMVQLLSPIVDQKSHTVEIKALVDNK
NKLLKPGMFIRANITTGQEQPTIRIPSNAVVLNEDGTTGIVCVVRNGKSYRNEVKLGSQSEGKIVISEGLTEGDVVLLER
ISQLRDGMSVNPYFDKR

Specific function: The periplasmic linker component of an organic solvent efflux pump. Involved in export of a number of organic solvents, including toluene and styrene. This is the most important solvent efflux pump in this strain, although it can export AMP and some antib

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=216, Percent_Identity=26.3888888888889, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789665, Length=217, Percent_Identity=26.2672811059908, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786668, Length=213, Percent_Identity=25.8215962441315, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 55820; Mature: 55820

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKFLKSKFGKFFLLSLAAYFIISLFLTRLAKNSQSKMLKNSVSMLTKPLSFSLSSPNQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCC
SEETFSSEEESQNLMSILAMNVVKESITPTVEASGMIDFIEKVDVYSKVSGRIEKVYFKE
CHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCC
GEEISQNQRLFKMETLPLELELLKQESTLESSKSQVKLAKEKYLKAKNNVLAKIQEYEKS
CCHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSVLEKSKQEYEKAKNSFAGIEEIYNAGGFTKEEFENAKLNLNSKETSYQIAEKDVEIRS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEECCCCCCHHHHHHCCCEEEE
IGLTDKDILRNNYKLPQTKEERTTILEEINSLIEKAEWEVAEGVYKSHEAQVNSTKMMLK
ECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ESLVFSPLTGVISKQFKTEGEILSGSGPGQHVVSVINVKQVYAVLNIPESESINLKKGMK
HHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCC
VSFLADVYKGQQFQGMVQLLSPIVDQKSHTVEIKALVDNKNKLLKPGMFIRANITTGQEQ
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEEEEEECCCCCC
PTIRIPSNAVVLNEDGTTGIVCVVRNGKSYRNEVKLGSQSEGKIVISEGLTEGDVVLLER
CEEECCCCEEEEECCCCCEEEEEEECCCCHHHHCCCCCCCCCCEEEECCCCCCHHHHHHH
ISQLRDGMSVNPYFDKR
HHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MIIKFLKSKFGKFFLLSLAAYFIISLFLTRLAKNSQSKMLKNSVSMLTKPLSFSLSSPNQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCC
SEETFSSEEESQNLMSILAMNVVKESITPTVEASGMIDFIEKVDVYSKVSGRIEKVYFKE
CHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCC
GEEISQNQRLFKMETLPLELELLKQESTLESSKSQVKLAKEKYLKAKNNVLAKIQEYEKS
CCHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSVLEKSKQEYEKAKNSFAGIEEIYNAGGFTKEEFENAKLNLNSKETSYQIAEKDVEIRS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEECCCCCCHHHHHHCCCEEEE
IGLTDKDILRNNYKLPQTKEERTTILEEINSLIEKAEWEVAEGVYKSHEAQVNSTKMMLK
ECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ESLVFSPLTGVISKQFKTEGEILSGSGPGQHVVSVINVKQVYAVLNIPESESINLKKGMK
HHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCC
VSFLADVYKGQQFQGMVQLLSPIVDQKSHTVEIKALVDNKNKLLKPGMFIRANITTGQEQ
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEEEEEECCCCCC
PTIRIPSNAVVLNEDGTTGIVCVVRNGKSYRNEVKLGSQSEGKIVISEGLTEGDVVLLER
CEEECCCCEEEEECCCCCEEEEEEECCCCHHHHCCCCCCCCCCEEEECCCCCCHHHHHHH
ISQLRDGMSVNPYFDKR
HHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11395460 [H]