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The map label for this gene is purD

Identifier: 45656370

GI number: 45656370

Start: 544669

End: 545949

Strand: Reverse

Name: purD

Synonym: LIC10472

Alternate gene names: 45656370

Gene position: 545949-544669 (Counterclockwise)

Preceding gene: 45656371

Following gene: 45656369

Centisome position: 12.76

GC content: 40.67

Gene sequence:

>1281_bases
TTGCAGGTTAAGTTGAAAGTCCTTCTTATCGGTTCTGGCGGTAGAGAAAGTGCAATCGCCTTTTATCTCCGTAAATCTGT
TTTGTTAAGCGAATTGAAAGTTTTTCCTGGAAATGGCGGTTTTCCGGACCAAGAACTTCTTCCTCCTGATTCGTTCCAAG
TTTTGGACAAGAATTCGGTTCAGTCCTTTTTAAAACAAAATCCTTTCGATTTGATTGTAGTCGGTCCGGAAGATCCATTA
GTGGCGGGTTTTGCGGATTGGGCCGCAGAACTAAATATTCCGGTTTTTGGCCCCGATTCGTTTTGCGCCCAAGTAGAAGG
TTCTAAAGATTTTGCAAAATCTTTGATGACTGAAGCTAAGATTCCCACCGCAGAATATAAAACATTTTCCGAATATTCTG
ATTCTTTAAAATACCTTGAGTCCAAATCGATTCCGATCGTTATCAAAGCAGATGGTCTGGCAGCAGGCAAAGGTGTTACT
GTAGCGACTTCAAAAGAAATGGCCCAAACCGCACTTAAAGAAATTTTTAAAGATAAAAAATTTGGTTCCAGTGGCAATCA
AGTAGTCATCGAAGAATTTATGGAAGGTCAAGAAGCGTCTATCTTTGCGATTTCAGACGGAGATTCCTATTTCCTTTTAC
CTGCAGCTCAGGATCATAAAAGAGCATTTGACGGTGATCAAGGTCCGAACACCGGAGGTATGGGCGCTTATTGTCCTGCG
CCGGTAATTTCCGAAAGTATATTACAAAAAGTGAAAGAACAAATTTTCGATCCTATGTTTGATCTATTCCGCAAAAAAGG
ACATCCTTATCGCGGCCTTCTTTACGCCGGTTTGATGATTTCTCCAAACGGAGAACCCAAAGTTGTTGAGTTCAATTGTA
GATTTGGAGATCCGGAAACCCAGTGTGTTCTTGCAATGTTAGACGGAGATCTATTAGAACTTCTTTATAGAGCTTCCACT
GGTAAAATCAAAGGGATTCAAGCAGCCGTTAAAAAAGGCGCGGCTGTTGTTGTTGTGCTTGCCGCGCAAGGATATCCCGA
TTTTTATGAAAAAAATATTCCACTCAATCTTCCAGAAACTTCGGGTCAAAACGTTCATCTTTTTCATGCGGGAACTTTAA
AAAAAGATGGAAAAGTTTTTTCATCTGGGGGAAGAATTCTCGGAATAGTAGCTCAAGGTGCCGATCTAAAAAGTTCGGTA
GATCAAGCTTATTCCTTCCTGGAAAAAATCCAGGCTCCCAAAACGTTTTATAGAAAAGATATCGGATATAGAGCCCTTTA
A

Upstream 100 bases:

>100_bases
TTTCTTTCTAAAGCAGCCCCAGAAGTTGTGGAAAAAGAAAGAGATAAACTAAAAACTCTCATTGATAAAGTAGAAGTTCT
AAAAAAGGGGATTCAAAATC

Downstream 100 bases:

>100_bases
CATGCCATTTTTGATTTCGGGAAATTTAGATCTCGTAGAAAGACATAGCCTCAAGAAAATGGAAAAATTTCTTGGGATCA
TTTTATCTCCGGTTCAGTTT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MQVKLKVLLIGSGGRESAIAFYLRKSVLLSELKVFPGNGGFPDQELLPPDSFQVLDKNSVQSFLKQNPFDLIVVGPEDPL
VAGFADWAAELNIPVFGPDSFCAQVEGSKDFAKSLMTEAKIPTAEYKTFSEYSDSLKYLESKSIPIVIKADGLAAGKGVT
VATSKEMAQTALKEIFKDKKFGSSGNQVVIEEFMEGQEASIFAISDGDSYFLLPAAQDHKRAFDGDQGPNTGGMGAYCPA
PVISESILQKVKEQIFDPMFDLFRKKGHPYRGLLYAGLMISPNGEPKVVEFNCRFGDPETQCVLAMLDGDLLELLYRAST
GKIKGIQAAVKKGAAVVVVLAAQGYPDFYEKNIPLNLPETSGQNVHLFHAGTLKKDGKVFSSGGRILGIVAQGADLKSSV
DQAYSFLEKIQAPKTFYRKDIGYRAL

Sequences:

>Translated_426_residues
MQVKLKVLLIGSGGRESAIAFYLRKSVLLSELKVFPGNGGFPDQELLPPDSFQVLDKNSVQSFLKQNPFDLIVVGPEDPL
VAGFADWAAELNIPVFGPDSFCAQVEGSKDFAKSLMTEAKIPTAEYKTFSEYSDSLKYLESKSIPIVIKADGLAAGKGVT
VATSKEMAQTALKEIFKDKKFGSSGNQVVIEEFMEGQEASIFAISDGDSYFLLPAAQDHKRAFDGDQGPNTGGMGAYCPA
PVISESILQKVKEQIFDPMFDLFRKKGHPYRGLLYAGLMISPNGEPKVVEFNCRFGDPETQCVLAMLDGDLLELLYRAST
GKIKGIQAAVKKGAAVVVVLAAQGYPDFYEKNIPLNLPETSGQNVHLFHAGTLKKDGKVFSSGGRILGIVAQGADLKSSV
DQAYSFLEKIQAPKTFYRKDIGYRAL
>Mature_426_residues
MQVKLKVLLIGSGGRESAIAFYLRKSVLLSELKVFPGNGGFPDQELLPPDSFQVLDKNSVQSFLKQNPFDLIVVGPEDPL
VAGFADWAAELNIPVFGPDSFCAQVEGSKDFAKSLMTEAKIPTAEYKTFSEYSDSLKYLESKSIPIVIKADGLAAGKGVT
VATSKEMAQTALKEIFKDKKFGSSGNQVVIEEFMEGQEASIFAISDGDSYFLLPAAQDHKRAFDGDQGPNTGGMGAYCPA
PVISESILQKVKEQIFDPMFDLFRKKGHPYRGLLYAGLMISPNGEPKVVEFNCRFGDPETQCVLAMLDGDLLELLYRAST
GKIKGIQAAVKKGAAVVVVLAAQGYPDFYEKNIPLNLPETSGQNVHLFHAGTLKKDGKVFSSGGRILGIVAQGADLKSSV
DQAYSFLEKIQAPKTFYRKDIGYRAL

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=42.2169811320755, Blast_Score=355, Evalue=6e-98,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=42.2169811320755, Blast_Score=355, Evalue=6e-98,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=42.2169811320755, Blast_Score=355, Evalue=6e-98,
Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=42.2169811320755, Blast_Score=354, Evalue=7e-98,
Organism=Escherichia coli, GI1790438, Length=426, Percent_Identity=43.8967136150235, Blast_Score=352, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI17567511, Length=426, Percent_Identity=35.9154929577465, Blast_Score=246, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6321203, Length=440, Percent_Identity=39.5454545454545, Blast_Score=310, Evalue=3e-85,
Organism=Drosophila melanogaster, GI62471619, Length=434, Percent_Identity=39.63133640553, Blast_Score=281, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24582400, Length=434, Percent_Identity=39.63133640553, Blast_Score=281, Evalue=5e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_LEPIC (Q72V31)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_000456.1
- ProteinModelPortal:   Q72V31
- SMR:   Q72V31
- GeneID:   2771801
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC10472
- NMPDR:   fig|267671.1.peg.456
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK13789
- BioCyc:   LINT267671:LIC_10472-MONOMER
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 46259; Mature: 46259

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVKLKVLLIGSGGRESAIAFYLRKSVLLSELKVFPGNGGFPDQELLPPDSFQVLDKNSV
CCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCEECCHHHH
QSFLKQNPFDLIVVGPEDPLVAGFADWAAELNIPVFGPDSFCAQVEGSKDFAKSLMTEAK
HHHHHCCCCEEEEECCCCCEEECHHHHHHHCCCEEECCCHHHEECCCCHHHHHHHHHHCC
IPTAEYKTFSEYSDSLKYLESKSIPIVIKADGLAAGKGVTVATSKEMAQTALKEIFKDKK
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECHHHHHHHHHHHHHHHCC
FGSSGNQVVIEEFMEGQEASIFAISDGDSYFLLPAAQDHKRAFDGDQGPNTGGMGAYCPA
CCCCCCEEHHHHHHCCCCCEEEEEECCCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCC
PVISESILQKVKEQIFDPMFDLFRKKGHPYRGLLYAGLMISPNGEPKVVEFNCRFGDPET
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCEEEEEEECCCCCCH
QCVLAMLDGDLLELLYRASTGKIKGIQAAVKKGAAVVVVLAAQGYPDFYEKNIPLNLPET
HEEEEECCCHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEEECCCCHHHHCCCCCCCCCC
SGQNVHLFHAGTLKKDGKVFSSGGRILGIVAQGADLKSSVDQAYSFLEKIQAPKTFYRKD
CCCEEEEEECCCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHC
IGYRAL
CCCCCC
>Mature Secondary Structure
MQVKLKVLLIGSGGRESAIAFYLRKSVLLSELKVFPGNGGFPDQELLPPDSFQVLDKNSV
CCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCEECCHHHH
QSFLKQNPFDLIVVGPEDPLVAGFADWAAELNIPVFGPDSFCAQVEGSKDFAKSLMTEAK
HHHHHCCCCEEEEECCCCCEEECHHHHHHHCCCEEECCCHHHEECCCCHHHHHHHHHHCC
IPTAEYKTFSEYSDSLKYLESKSIPIVIKADGLAAGKGVTVATSKEMAQTALKEIFKDKK
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECHHHHHHHHHHHHHHHCC
FGSSGNQVVIEEFMEGQEASIFAISDGDSYFLLPAAQDHKRAFDGDQGPNTGGMGAYCPA
CCCCCCEEHHHHHHCCCCCEEEEEECCCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCC
PVISESILQKVKEQIFDPMFDLFRKKGHPYRGLLYAGLMISPNGEPKVVEFNCRFGDPET
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCEEEEEEECCCCCCH
QCVLAMLDGDLLELLYRASTGKIKGIQAAVKKGAAVVVVLAAQGYPDFYEKNIPLNLPET
HEEEEECCCHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEEECCCCHHHHCCCCCCCCCC
SGQNVHLFHAGTLKKDGKVFSSGGRILGIVAQGADLKSSVDQAYSFLEKIQAPKTFYRKD
CCCEEEEEECCCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHC
IGYRAL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA