Click here to switch to the map view.

The map label for this gene is rep [H]

Identifier: 45656320

GI number: 45656320

Start: 478459

End: 479760

Strand: Reverse

Name: rep [H]

Synonym: LIC10420

Alternate gene names: 45656320

Gene position: 479760-478459 (Counterclockwise)

Preceding gene: 45656322

Following gene: 45656318

Centisome position: 11.22

GC content: 33.26

Gene sequence:

>1302_bases
ATGAAAAACAAAGTTCAATACAGTTCAGCACAACAAAAAGTAATCAATGAAAACACAAGATTTGTACAAGTAGTCGCCGC
CGCAGGTTCTGGTAAAACGAGCACGATGGTTGGGATTATCGAAAGAATTTTAGTTGAAAACTTATTTCCAAAAGAATCCG
TTTTGGTTTTAACCTTTTCGCGCAAAGCCGCAATAGAAATTTCAAATAGGATTCAAAAAGTCACAGATAAAAATTCTATC
AGAGTTCAAACATTTCATGCGTATTGTTTGTATGCTCTCAGCCAATGGCATCCTAAATTTACATTAAAAAAACCTAAAAT
TCTTTCTCCAGAAGAAAAAAATCAATTCTACCGAGGATTCTTAAAAAAAGAACGTAATAAAATCGGCGGAATTCCGTATG
ATTTTTTTTGGGCGGAAAATATTCCTTTTATCCAAGAAAATTTTTCAGAACTCAAAAAAGATTTAGAATTTGCTTATCAA
AAATTTAAATATAACAACGGCTTTTTAGATTTTGAAGACTTAGTAAAAATGTTCTTAGACGGTCTTAAAAACGAAGAGGA
GTGGACCTCTGAACCCAGAAGCCTCCTCCAAAAAATCATCGTAGATGAATTTCAAGATACGGATTTAGAACAACTTGAAT
TTCTAAAGTTGTTATCTCAAAGAGCGTCTATCGTTGTCGTAGGAGACGACAGCCAAGCGATTTATAGTTTTCGAGGAACT
TCTCCCGAAGCTTTTTTAAACTTTCAACATCTTTTCCAACCCTGTAAGGTTCACTTTTTAAACACAAATTACCGATCTCT
TCCGGAAATTATACACACATCCTCGATTCCAATCCAAAAAAATCATCATAAAATCAACAAAGAAGTTTTCCCTTTTCGTC
ACGAAAAAGGTTTTGTGGGAAAGATCTTCATCGAAGAAGCAGCTGACCTAATTCCATTTCTTAACCGAGCGATCCTTACT
TCAAAAGACGATTTTAAAATTTTATGTAGATCTAATTTTAGGATTTCTGAATATATTAGAGAAGGTATTCCAAAACGTTA
TCTAATGACGATCCATGCAAGCAAAGGTTTAGAATTCCATACTGTATTTGTAGACGTTGCAGATGGTTGGAACGCAAGAC
TCGATTCTACTTTAAAGACTATTGAAGAAGAAAGAAGAATTCTATATGTTGGCTTATCAAGAGCCAAAGATCGTTTATTG
ATCTTAGGAACCAGTAAAAATTCCAGAAGAGAAACGATAGAAAATATATTTTTCCATTATTTCAAAAAACTCAAAAATAT
AGTTCCGGAAGATTTAATCTAA

Upstream 100 bases:

>100_bases
AGTTTATTTTCTGTATTCATTTTCATACGTTTTTGTAAAAACATTAAAAAAATAGAACTGTGCTTTTGACTCTCGGAACC
GTATTCGATAAGAGGAAAAA

Downstream 100 bases:

>100_bases
GATTTTTTTTACCCATGCTCATACAAAAACAAAAGCCGATCGCATGTTCTTTTACCGTAGCCACTTTAGATTTAAAAATA
ATTTTCTGAATTCTATTTTT

Product: DNA helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MKNKVQYSSAQQKVINENTRFVQVVAAAGSGKTSTMVGIIERILVENLFPKESVLVLTFSRKAAIEISNRIQKVTDKNSI
RVQTFHAYCLYALSQWHPKFTLKKPKILSPEEKNQFYRGFLKKERNKIGGIPYDFFWAENIPFIQENFSELKKDLEFAYQ
KFKYNNGFLDFEDLVKMFLDGLKNEEEWTSEPRSLLQKIIVDEFQDTDLEQLEFLKLLSQRASIVVVGDDSQAIYSFRGT
SPEAFLNFQHLFQPCKVHFLNTNYRSLPEIIHTSSIPIQKNHHKINKEVFPFRHEKGFVGKIFIEEAADLIPFLNRAILT
SKDDFKILCRSNFRISEYIREGIPKRYLMTIHASKGLEFHTVFVDVADGWNARLDSTLKTIEEERRILYVGLSRAKDRLL
ILGTSKNSRRETIENIFFHYFKKLKNIVPEDLI

Sequences:

>Translated_433_residues
MKNKVQYSSAQQKVINENTRFVQVVAAAGSGKTSTMVGIIERILVENLFPKESVLVLTFSRKAAIEISNRIQKVTDKNSI
RVQTFHAYCLYALSQWHPKFTLKKPKILSPEEKNQFYRGFLKKERNKIGGIPYDFFWAENIPFIQENFSELKKDLEFAYQ
KFKYNNGFLDFEDLVKMFLDGLKNEEEWTSEPRSLLQKIIVDEFQDTDLEQLEFLKLLSQRASIVVVGDDSQAIYSFRGT
SPEAFLNFQHLFQPCKVHFLNTNYRSLPEIIHTSSIPIQKNHHKINKEVFPFRHEKGFVGKIFIEEAADLIPFLNRAILT
SKDDFKILCRSNFRISEYIREGIPKRYLMTIHASKGLEFHTVFVDVADGWNARLDSTLKTIEEERRILYVGLSRAKDRLL
ILGTSKNSRRETIENIFFHYFKKLKNIVPEDLI
>Mature_433_residues
MKNKVQYSSAQQKVINENTRFVQVVAAAGSGKTSTMVGIIERILVENLFPKESVLVLTFSRKAAIEISNRIQKVTDKNSI
RVQTFHAYCLYALSQWHPKFTLKKPKILSPEEKNQFYRGFLKKERNKIGGIPYDFFWAENIPFIQENFSELKKDLEFAYQ
KFKYNNGFLDFEDLVKMFLDGLKNEEEWTSEPRSLLQKIIVDEFQDTDLEQLEFLKLLSQRASIVVVGDDSQAIYSFRGT
SPEAFLNFQHLFQPCKVHFLNTNYRSLPEIIHTSSIPIQKNHHKINKEVFPFRHEKGFVGKIFIEEAADLIPFLNRAILT
SKDDFKILCRSNFRISEYIREGIPKRYLMTIHASKGLEFHTVFVDVADGWNARLDSTLKTIEEERRILYVGLSRAKDRLL
ILGTSKNSRRETIENIFFHYFKKLKNIVPEDLI

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=290, Percent_Identity=28.6206896551724, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI48994965, Length=358, Percent_Identity=26.2569832402235, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1787196, Length=472, Percent_Identity=25.635593220339, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322369, Length=326, Percent_Identity=25.1533742331288, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50618; Mature: 50618

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKVQYSSAQQKVINENTRFVQVVAAAGSGKTSTMVGIIERILVENLFPKESVLVLTFS
CCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEC
RKAAIEISNRIQKVTDKNSIRVQTFHAYCLYALSQWHPKFTLKKPKILSPEEKNQFYRGF
CCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHH
LKKERNKIGGIPYDFFWAENIPFIQENFSELKKDLEFAYQKFKYNNGFLDFEDLVKMFLD
HHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
GLKNEEEWTSEPRSLLQKIIVDEFQDTDLEQLEFLKLLSQRASIVVVGDDSQAIYSFRGT
HCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEECCC
SPEAFLNFQHLFQPCKVHFLNTNYRSLPEIIHTSSIPIQKNHHKINKEVFPFRHEKGFVG
CHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCEE
KIFIEEAADLIPFLNRAILTSKDDFKILCRSNFRISEYIREGIPKRYLMTIHASKGLEFH
EHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCCEEEEEEECCCCCEEE
TVFVDVADGWNARLDSTLKTIEEERRILYVGLSRAKDRLLILGTSKNSRRETIENIFFHY
EEEEECCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHH
FKKLKNIVPEDLI
HHHHHHCCCCCCC
>Mature Secondary Structure
MKNKVQYSSAQQKVINENTRFVQVVAAAGSGKTSTMVGIIERILVENLFPKESVLVLTFS
CCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEC
RKAAIEISNRIQKVTDKNSIRVQTFHAYCLYALSQWHPKFTLKKPKILSPEEKNQFYRGF
CCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHH
LKKERNKIGGIPYDFFWAENIPFIQENFSELKKDLEFAYQKFKYNNGFLDFEDLVKMFLD
HHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
GLKNEEEWTSEPRSLLQKIIVDEFQDTDLEQLEFLKLLSQRASIVVVGDDSQAIYSFRGT
HCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEECCC
SPEAFLNFQHLFQPCKVHFLNTNYRSLPEIIHTSSIPIQKNHHKINKEVFPFRHEKGFVG
CHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCEE
KIFIEEAADLIPFLNRAILTSKDDFKILCRSNFRISEYIREGIPKRYLMTIHASKGLEFH
EHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCCEEEEEEECCCCCEEE
TVFVDVADGWNARLDSTLKTIEEERRILYVGLSRAKDRLLILGTSKNSRRETIENIFFHY
EEEEECCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHH
FKKLKNIVPEDLI
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]