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The map label for this gene is aEF-1_alpha [H]

Identifier: 45358933

GI number: 45358933

Start: 1351135

End: 1352421

Strand: Direct

Name: aEF-1_alpha [H]

Synonym: MMP1370

Alternate gene names: 45358933

Gene position: 1351135-1352421 (Clockwise)

Preceding gene: 45358932

Following gene: 45358934

Centisome position: 81.34

GC content: 40.17

Gene sequence:

>1287_bases
ATGGCAAAAGAAAAACCAATTCTTAACGTTGCATTTATCGGACACGTAGATGCTGGTAAATCAACAACAGTAGGAAGATT
ATTATTAGATGGTGGAGCTATCGACCCACAGTTAATCGTAAGATTAAGAAAAGAAGCTGAAGAAAAAGGTAAAGCAGGAT
TCGAATTTGCTTACGTTATGGACGGTTTAAAGGAAGAAAGAGAAAGAGGGGTTACAATTGACGTAGCTCACAAAAAATTC
CCTACCGCAAAATACGAAGTTACAATCGTAGACTGCCCTGGCCACAGAGACTTCATTAAAAACATGATTACCGGTGCTTC
ACAAGCTGATGCAGCTGTTTTAGTTGTAAACGTTGACGATGCTAAATCAGGCATTCAACCCCAAACAAGAGAACACGTAT
TCTTATCAAGAACACTTGGTATCACACAATTAGCTGTTGCAATCAACAAAATGGACACAGTTAACTTCTCAGAAGCTGAC
TACAACGAAATGAAAAAGATGTTAGGCGACCAGTTGTTAAAAATGGTTGGATTCAACCCTGACAACATCGACTTCATACC
AGTTGCTTCACTTCTTGGTGACAACGTATTCAAAAAATCAGAAAACACACCATGGTACAACGGACCTACACTCGCACAAG
TTATCGATGGTTTCCAACCACCTGAAAAACCAACAACCTTACCATTAAGATTACCTATCCAAGATGTTTACTCAATCACT
GGTGTAGGTACAGTTCCAGTAGGAAGAGTTGAAACAGGTATCATCAAACCAGGAGACAAAGTTGTCTTCGAACCTGCAGG
AGCAGTAGGAGAAATCAAAACTGTGGAAATGCACCACGAACAATTACCAAGCGCTGAACCTGGAGACAACATCGGTTTCA
ACGTAAGAGGCGTTGGTAAAAAAGACATTAAAAGAGGAGACGTTTTAGGACACACAACAAACCCTCCAACAGTTGCTGCA
GACTTCACAGCACAAATCGTTGTTTTACAACACCCATCAGTTATGACAGTAGGTTACACACCTGTATTCCACGCACACAC
AGCACAGATTGCATGTACATTCATGGAATTACAGAAAAAATTAAACCCTGCAACTGGTGAAGTTTTAGAAGAAAACCCAG
ACTTCTTAAAAGCTGGTGACGCTGCAATCGTTAAATTAATGCCTACAAAACCATTAGTTATGGAAAGCGTTAAAGAAATC
CCACAACTCGGTAGATTCGCAATCAGAGATATGGGTATGACCGTTGCTGCAGGTATGGCAATCCAAGTTACAGCTAAAAA
CAAATAA

Upstream 100 bases:

>100_bases
GGTTAAAATCTGAATAAACAAGTACCATAAAATATATATACCCTTTTTCACTATCATATTACAAAAATCGCTAAAAATAT
ACTCTTATGGAGGAATGATT

Downstream 100 bases:

>100_bases
TTTAGCTTAACTTTTTCTTCTTATTAAACACGGCAAAAGAGGCATATTATGCAAAAAGCAAGAATAAAATTATCAAGCAC
ACAACACACAGAATTAGACG

Product: elongation factor 1-alpha

Products: GDP; phosphate

Alternate protein names: EF-1-alpha; Elongation factor Tu; EF-Tu [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MAKEKPILNVAFIGHVDAGKSTTVGRLLLDGGAIDPQLIVRLRKEAEEKGKAGFEFAYVMDGLKEERERGVTIDVAHKKF
PTAKYEVTIVDCPGHRDFIKNMITGASQADAAVLVVNVDDAKSGIQPQTREHVFLSRTLGITQLAVAINKMDTVNFSEAD
YNEMKKMLGDQLLKMVGFNPDNIDFIPVASLLGDNVFKKSENTPWYNGPTLAQVIDGFQPPEKPTTLPLRLPIQDVYSIT
GVGTVPVGRVETGIIKPGDKVVFEPAGAVGEIKTVEMHHEQLPSAEPGDNIGFNVRGVGKKDIKRGDVLGHTTNPPTVAA
DFTAQIVVLQHPSVMTVGYTPVFHAHTAQIACTFMELQKKLNPATGEVLEENPDFLKAGDAAIVKLMPTKPLVMESVKEI
PQLGRFAIRDMGMTVAAGMAIQVTAKNK

Sequences:

>Translated_428_residues
MAKEKPILNVAFIGHVDAGKSTTVGRLLLDGGAIDPQLIVRLRKEAEEKGKAGFEFAYVMDGLKEERERGVTIDVAHKKF
PTAKYEVTIVDCPGHRDFIKNMITGASQADAAVLVVNVDDAKSGIQPQTREHVFLSRTLGITQLAVAINKMDTVNFSEAD
YNEMKKMLGDQLLKMVGFNPDNIDFIPVASLLGDNVFKKSENTPWYNGPTLAQVIDGFQPPEKPTTLPLRLPIQDVYSIT
GVGTVPVGRVETGIIKPGDKVVFEPAGAVGEIKTVEMHHEQLPSAEPGDNIGFNVRGVGKKDIKRGDVLGHTTNPPTVAA
DFTAQIVVLQHPSVMTVGYTPVFHAHTAQIACTFMELQKKLNPATGEVLEENPDFLKAGDAAIVKLMPTKPLVMESVKEI
PQLGRFAIRDMGMTVAAGMAIQVTAKNK
>Mature_427_residues
AKEKPILNVAFIGHVDAGKSTTVGRLLLDGGAIDPQLIVRLRKEAEEKGKAGFEFAYVMDGLKEERERGVTIDVAHKKFP
TAKYEVTIVDCPGHRDFIKNMITGASQADAAVLVVNVDDAKSGIQPQTREHVFLSRTLGITQLAVAINKMDTVNFSEADY
NEMKKMLGDQLLKMVGFNPDNIDFIPVASLLGDNVFKKSENTPWYNGPTLAQVIDGFQPPEKPTTLPLRLPIQDVYSITG
VGTVPVGRVETGIIKPGDKVVFEPAGAVGEIKTVEMHHEQLPSAEPGDNIGFNVRGVGKKDIKRGDVLGHTTNPPTVAAD
FTAQIVVLQHPSVMTVGYTPVFHAHTAQIACTFMELQKKLNPATGEVLEENPDFLKAGDAAIVKLMPTKPLVMESVKEIP
QLGRFAIRDMGMTVAAGMAIQVTAKNK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG5256

COG function: function code J; Translation elongation factor EF-1alpha (GTPase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503471, Length=445, Percent_Identity=51.2359550561798, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI4503475, Length=446, Percent_Identity=50.4484304932735, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI223555963, Length=426, Percent_Identity=38.4976525821596, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI5729864, Length=426, Percent_Identity=38.4976525821596, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI194018522, Length=429, Percent_Identity=36.1305361305361, Blast_Score=268, Evalue=5e-72,
Organism=Homo sapiens, GI194097354, Length=429, Percent_Identity=36.1305361305361, Blast_Score=268, Evalue=6e-72,
Organism=Homo sapiens, GI194018520, Length=429, Percent_Identity=36.1305361305361, Blast_Score=268, Evalue=6e-72,
Organism=Homo sapiens, GI46094014, Length=429, Percent_Identity=35.4312354312354, Blast_Score=265, Evalue=8e-71,
Organism=Homo sapiens, GI34147630, Length=436, Percent_Identity=31.651376146789, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI54607086, Length=353, Percent_Identity=25.7790368271955, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI82546879, Length=408, Percent_Identity=25.2450980392157, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI157426893, Length=261, Percent_Identity=28.735632183908, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789108, Length=429, Percent_Identity=32.6340326340326, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1790412, Length=438, Percent_Identity=33.3333333333333, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1789737, Length=436, Percent_Identity=33.4862385321101, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI2367247, Length=329, Percent_Identity=27.9635258358663, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI48994988, Length=261, Percent_Identity=29.1187739463602, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790835, Length=142, Percent_Identity=30.9859154929577, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788922, Length=263, Percent_Identity=26.9961977186312, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17552884, Length=438, Percent_Identity=52.0547945205479, Blast_Score=454, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI17569207, Length=438, Percent_Identity=52.0547945205479, Blast_Score=454, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI32566303, Length=434, Percent_Identity=49.3087557603687, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI115532067, Length=427, Percent_Identity=36.0655737704918, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI115532065, Length=427, Percent_Identity=36.0655737704918, Blast_Score=272, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI32566629, Length=426, Percent_Identity=33.8028169014084, Blast_Score=249, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI32566301, Length=148, Percent_Identity=58.1081081081081, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17556456, Length=441, Percent_Identity=33.5600907029478, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI25141371, Length=306, Percent_Identity=33.0065359477124, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17565438, Length=373, Percent_Identity=25.7372654155496, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17506081, Length=292, Percent_Identity=23.6301369863014, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=255, Percent_Identity=29.8039215686275, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32566299, Length=64, Percent_Identity=51.5625, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17556745, Length=212, Percent_Identity=31.6037735849057, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6325337, Length=436, Percent_Identity=52.0642201834862, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6319594, Length=436, Percent_Identity=52.0642201834862, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6320377, Length=426, Percent_Identity=34.7417840375587, Blast_Score=242, Evalue=8e-65,
Organism=Saccharomyces cerevisiae, GI6322937, Length=471, Percent_Identity=29.723991507431, Blast_Score=207, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6324761, Length=435, Percent_Identity=31.7241379310345, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323320, Length=328, Percent_Identity=28.9634146341463, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24652838, Length=446, Percent_Identity=52.6905829596413, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17137572, Length=446, Percent_Identity=52.6905829596413, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI45553807, Length=445, Percent_Identity=51.9101123595506, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI45553816, Length=445, Percent_Identity=51.9101123595506, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651721, Length=445, Percent_Identity=51.9101123595506, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI17864154, Length=445, Percent_Identity=51.9101123595506, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI45550900, Length=429, Percent_Identity=35.1981351981352, Blast_Score=278, Evalue=4e-75,
Organism=Drosophila melanogaster, GI17137380, Length=426, Percent_Identity=34.9765258215962, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI281363316, Length=424, Percent_Identity=33.9622641509434, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI17864358, Length=424, Percent_Identity=33.9622641509434, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI19921738, Length=426, Percent_Identity=29.81220657277, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI19922512, Length=415, Percent_Identity=23.3734939759036, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24654852, Length=415, Percent_Identity=23.3734939759036, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24647048, Length=368, Percent_Identity=25.5434782608696, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24647046, Length=368, Percent_Identity=25.5434782608696, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR009001
- InterPro:   IPR004539
- InterPro:   IPR004161
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 46397; Mature: 46266

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKPILNVAFIGHVDAGKSTTVGRLLLDGGAIDPQLIVRLRKEAEEKGKAGFEFAYVM
CCCCCCEEEEEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
DGLKEERERGVTIDVAHKKFPTAKYEVTIVDCPGHRDFIKNMITGASQADAAVLVVNVDD
HHHHHHHHCCCEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCEEEEEEECC
AKSGIQPQTREHVFLSRTLGITQLAVAINKMDTVNFSEADYNEMKKMLGDQLLKMVGFNP
CCCCCCCCCHHEEEEEHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
DNIDFIPVASLLGDNVFKKSENTPWYNGPTLAQVIDGFQPPEKPTTLPLRLPIQDVYSIT
CCCEEEEHHHHHCCHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEECCHHHHHHHC
GVGTVPVGRVETGIIKPGDKVVFEPAGAVGEIKTVEMHHEQLPSAEPGDNIGFNVRGVGK
CCCCCCCCCCEECEECCCCEEEEECCCCCCCEEEEEEHHHHCCCCCCCCCCCEEEECCCH
KDIKRGDVLGHTTNPPTVAADFTAQIVVLQHPSVMTVGYTPVFHAHTAQIACTFMELQKK
HHCCCCCCCCCCCCCCEEEECCEEEEEEEECCCEEEECCCCEEECCHHHHHHHHHHHHHH
LNPATGEVLEENPDFLKAGDAAIVKLMPTKPLVMESVKEIPQLGRFAIRDMGMTVAAGMA
CCCCCCHHHHCCCCCEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCEE
IQVTAKNK
EEEEECCC
>Mature Secondary Structure 
AKEKPILNVAFIGHVDAGKSTTVGRLLLDGGAIDPQLIVRLRKEAEEKGKAGFEFAYVM
CCCCCEEEEEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
DGLKEERERGVTIDVAHKKFPTAKYEVTIVDCPGHRDFIKNMITGASQADAAVLVVNVDD
HHHHHHHHCCCEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCEEEEEEECC
AKSGIQPQTREHVFLSRTLGITQLAVAINKMDTVNFSEADYNEMKKMLGDQLLKMVGFNP
CCCCCCCCCHHEEEEEHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
DNIDFIPVASLLGDNVFKKSENTPWYNGPTLAQVIDGFQPPEKPTTLPLRLPIQDVYSIT
CCCEEEEHHHHHCCHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEECCHHHHHHHC
GVGTVPVGRVETGIIKPGDKVVFEPAGAVGEIKTVEMHHEQLPSAEPGDNIGFNVRGVGK
CCCCCCCCCCEECEECCCCEEEEECCCCCCCEEEEEEHHHHCCCCCCCCCCCEEEECCCH
KDIKRGDVLGHTTNPPTVAADFTAQIVVLQHPSVMTVGYTPVFHAHTAQIACTFMELQKK
HHCCCCCCCCCCCCCCEEEECCEEEEEEEECCCEEEECCCCEEECCHHHHHHHHHHHHHH
LNPATGEVLEENPDFLKAGDAAIVKLMPTKPLVMESVKEIPQLGRFAIRDMGMTVAAGMA
CCCCCCHHHHCCCCCEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCEE
IQVTAKNK
EEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA