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The map label for this gene is hybD [C]

Identifier: 45358900

GI number: 45358900

Start: 1318233

End: 1318715

Strand: Direct

Name: hybD [C]

Synonym: MMP1337

Alternate gene names: 45358900

Gene position: 1318233-1318715 (Clockwise)

Preceding gene: 45358897

Following gene: 45358901

Centisome position: 79.36

GC content: 33.33

Gene sequence:

>483_bases
ATGATCCCTGATTCTTTAAAATACGAAATTTTAGTTTTTGGCTGTGGAAATTTAATATTTGCAGATGATGGTTTTGGATA
CGAAGTAATTTCAAAGCTTGAAAAGATGGAACTCCCAGAAAACGTTGGAATAATCGATGCTGGAACTGGGGCACCTTATT
ATTTAATGTCCTTGATGGATGAAGAGTGCAATACTAAAAAAATTATTATTGTTGATATTATAGATTTTCAGCTCCCTCCA
GGAACTTTAAAAATATTATCAACTGAAGATCTCACAAAAATAGACAAATACACCTTTGATGCACATGATATGCCTCTTTC
AGACTATTTAATAAAAGCAAAAGAATCTGGAATCGATGTAACAGTTATCGGATGTCAAGCAAAACGGGTAACAATGCCTG
ATATCGAAGTTGGATTGACTGACGAAGTTAAAGCATCCCTTGACGATGCAGTAAAACTTGTTCTTGAAAAAATAAACGAA
TAA

Upstream 100 bases:

>100_bases
ACCCTTATAACCGATTCCTATAAAATAATATACCTAGATTATATATGCACTTAGATATAAAAGGATAAATAAATTTTGAA
CTGATTTTTTGGTGATAGAT

Downstream 100 bases:

>100_bases
CTTAACAAATTTAAAAATTAAGAAATATTTCAAAAATACTTTTTTTAAATTAGATTATTTTAATCAATCAAAAATTACTT
TAACCCGTTTAAAACACAAA

Product: hydrogenase maturation protease

Products: reduced coenzyme F420

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MIPDSLKYEILVFGCGNLIFADDGFGYEVISKLEKMELPENVGIIDAGTGAPYYLMSLMDEECNTKKIIIVDIIDFQLPP
GTLKILSTEDLTKIDKYTFDAHDMPLSDYLIKAKESGIDVTVIGCQAKRVTMPDIEVGLTDEVKASLDDAVKLVLEKINE

Sequences:

>Translated_160_residues
MIPDSLKYEILVFGCGNLIFADDGFGYEVISKLEKMELPENVGIIDAGTGAPYYLMSLMDEECNTKKIIIVDIIDFQLPP
GTLKILSTEDLTKIDKYTFDAHDMPLSDYLIKAKESGIDVTVIGCQAKRVTMPDIEVGLTDEVKASLDDAVKLVLEKINE
>Mature_160_residues
MIPDSLKYEILVFGCGNLIFADDGFGYEVISKLEKMELPENVGIIDAGTGAPYYLMSLMDEECNTKKIIIVDIIDFQLPP
GTLKILSTEDLTKIDKYTFDAHDMPLSDYLIKAKESGIDVTVIGCQAKRVTMPDIEVGLTDEVKASLDDAVKLVLEKINE

Specific function: Protease Involved In The C-Terminal Processing Of Hybc, The Large Subunit Of Hydrogenase 2. Specifically Cleaves Off A 15 Amino Acid Peptide From The C Terminus Of The Precursor Of Hybc. [C]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789367, Length=157, Percent_Identity=29.2993630573248, Blast_Score=62, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004411
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 1.12.98.1

Molecular weight: Translated: 17712; Mature: 17712

Theoretical pI: Translated: 4.06; Mature: 4.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPDSLKYEILVFGCGNLIFADDGFGYEVISKLEKMELPENVGIIDAGTGAPYYLMSLMD
CCCCCCEEEEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHH
EECNTKKIIIVDIIDFQLPPGTLKILSTEDLTKIDKYTFDAHDMPLSDYLIKAKESGIDV
CCCCCCEEEEEEEEECCCCCCEEEEEECCCCHHHHHEECCCCCCCHHHHHEECCCCCCEE
TVIGCQAKRVTMPDIEVGLTDEVKASLDDAVKLVLEKINE
EEEECCCCEEECCCCEECCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIPDSLKYEILVFGCGNLIFADDGFGYEVISKLEKMELPENVGIIDAGTGAPYYLMSLMD
CCCCCCEEEEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHH
EECNTKKIIIVDIIDFQLPPGTLKILSTEDLTKIDKYTFDAHDMPLSDYLIKAKESGIDV
CCCCCCEEEEEEEEECCCCCCEEEEEECCCCHHHHHEECCCCCCCHHHHHEECCCCCCEE
TVIGCQAKRVTMPDIEVGLTDEVKASLDDAVKLVLEKINE
EEEECCCCEEECCCCEECCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2; coenzyme F420

Specific reaction: H2 + coenzyme F420 = reduced coenzyme F420

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]