Click here to switch to the map view.

The map label for this gene is menE

Identifier: 41410136

GI number: 41410136

Start: 4499460

End: 4500551

Strand: Reverse

Name: menE

Synonym: MAP4038c

Alternate gene names: 41410136

Gene position: 4500551-4499460 (Counterclockwise)

Preceding gene: 41410137

Following gene: 41410135

Centisome position: 93.18

GC content: 76.19

Gene sequence:

>1092_bases
GTGCTGGACGGGCGCGATCCCGCCCTGGTGGTGCTCGGCCCGCCCGGCGACCGCGAGTCGGCGACGCTTCGGGCGGGGCT
GCGGGTCGGCGAACCCGTCGATGACGACGTGGCCCTGGTGGCGGCGACGTCGGGCACCACCGGTGCTCCCAAGGGTGCGA
TGCTGACCGCCGCCGCGCTGAGGGCGAGCGCGACGGCCACGCACGAGCGTCTGGGCGGGCCGGGCAGCTGGCTGCTGGCC
CTGCCGGCACACCACATCGCCGGGGTGCAGGTGCTGGTGCGCAGCCTGCTGGCCGGCTCGACGCCCGTCGAACTCGACGT
CTCCCGAGGCTTCGATGTCACCCAATTGCCCGCTGCGACAAGGGCATTGGGCACCGGCCGCCGGTACGCGTCGCTGGTGG
CCGTGCAGCTGGCCAAGGCGCTGGGTGACCCGGCGGCCACGGCCGCGCTGGCCGAACTGGACGCCGTGCTGCTGGGGGGC
GGGCCCGCGCCGCGGCCGGTGCTGCAGGCGGCCGCCGCCGCGGGCATCACGGTGGTGCGCAGCTACGGGATGAGCGAGAC
CGCCGGCGGGTGCGTGTACGACGGCGTCCCGCTGGACGGGGTGCGAGTGCGGGTGAGCGACGGGCGCATCGCGCTGGGCG
GCGCGACGTTGGCCAAGGGCTACCGCAATCCGGTCGATCCCGATCCGTTCGCCGAGCCGGGCTGGTTCCTCACCGACGAT
CTGGGCGCCGTCGACGACGACGGCGTGCTCACCGTGTTCGGCCGCGCCGACGACGCGATCAGCACCGGCGGGCTGACCGT
GCTGCCGCAACCCGTCGAGGCGGCGCTGTGCACGCATCCCGCGGTGGCCGACTGCGCGGTGTTCGGGCTGCCCGACGACC
GGCTGGGGCAGCGGGTGGTCGCCGCGATCGTGCTGCGCGACGGCCGCGCGGCGCCGAGCCCGGACGCGCTGCGCGCCCAC
CTGAGCCGCACCCTGGACGCCACCGCCGCGCCGCGCGAGGTACACGTTGTCGCCGCGCTGCCGCGCCGGGGCATCGGCAA
GGTGGACCGGGCGGCGTTGGTGCGCCGATTCGCCGGGGGCGGCGAACAATAG

Upstream 100 bases:

>100_bases
ACGACGCCGACCAGGCCGGGGACCCGGCATAGCCGCACTGCGCGCGCTGCACGTCCCGCCCGGCGCGGCGGTCCCGACGC
TGCTGCCGGCGCTGCGGCGG

Downstream 100 bases:

>100_bases
GCTGTTGCTCCGTGAGAATCGCTCGGCGGGACGCGGTGGCGGCCGCGGTCGGGGCGGCGTTGGTGGCGGCGGCGTTCCTG
CTGCCGCGGCTGAACCTCGG

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MLDGRDPALVVLGPPGDRESATLRAGLRVGEPVDDDVALVAATSGTTGAPKGAMLTAAALRASATATHERLGGPGSWLLA
LPAHHIAGVQVLVRSLLAGSTPVELDVSRGFDVTQLPAATRALGTGRRYASLVAVQLAKALGDPAATAALAELDAVLLGG
GPAPRPVLQAAAAAGITVVRSYGMSETAGGCVYDGVPLDGVRVRVSDGRIALGGATLAKGYRNPVDPDPFAEPGWFLTDD
LGAVDDDGVLTVFGRADDAISTGGLTVLPQPVEAALCTHPAVADCAVFGLPDDRLGQRVVAAIVLRDGRAAPSPDALRAH
LSRTLDATAAPREVHVVAALPRRGIGKVDRAALVRRFAGGGEQ

Sequences:

>Translated_363_residues
MLDGRDPALVVLGPPGDRESATLRAGLRVGEPVDDDVALVAATSGTTGAPKGAMLTAAALRASATATHERLGGPGSWLLA
LPAHHIAGVQVLVRSLLAGSTPVELDVSRGFDVTQLPAATRALGTGRRYASLVAVQLAKALGDPAATAALAELDAVLLGG
GPAPRPVLQAAAAAGITVVRSYGMSETAGGCVYDGVPLDGVRVRVSDGRIALGGATLAKGYRNPVDPDPFAEPGWFLTDD
LGAVDDDGVLTVFGRADDAISTGGLTVLPQPVEAALCTHPAVADCAVFGLPDDRLGQRVVAAIVLRDGRAAPSPDALRAH
LSRTLDATAAPREVHVVAALPRRGIGKVDRAALVRRFAGGGEQ
>Mature_363_residues
MLDGRDPALVVLGPPGDRESATLRAGLRVGEPVDDDVALVAATSGTTGAPKGAMLTAAALRASATATHERLGGPGSWLLA
LPAHHIAGVQVLVRSLLAGSTPVELDVSRGFDVTQLPAATRALGTGRRYASLVAVQLAKALGDPAATAALAELDAVLLGG
GPAPRPVLQAAAAAGITVVRSYGMSETAGGCVYDGVPLDGVRVRVSDGRIALGGATLAKGYRNPVDPDPFAEPGWFLTDD
LGAVDDDGVLTVFGRADDAISTGGLTVLPQPVEAALCTHPAVADCAVFGLPDDRLGQRVVAAIVLRDGRAAPSPDALRAH
LSRTLDATAAPREVHVVAALPRRGIGKVDRAALVRRFAGGGEQ

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA). May be involved in the biosynthesis of menaquinone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=386, Percent_Identity=29.7927461139896, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI187761343, Length=386, Percent_Identity=29.7927461139896, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI115511026, Length=361, Percent_Identity=28.2548476454294, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI58082049, Length=231, Percent_Identity=29.4372294372294, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI157311624, Length=122, Percent_Identity=35.2459016393443, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI157311622, Length=122, Percent_Identity=35.2459016393443, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI122937307, Length=122, Percent_Identity=36.0655737704918, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI38505220, Length=122, Percent_Identity=36.0655737704918, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI42544132, Length=127, Percent_Identity=33.0708661417323, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI28416953, Length=373, Percent_Identity=25.7372654155496, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI13375727, Length=124, Percent_Identity=34.6774193548387, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1788595, Length=272, Percent_Identity=32.7205882352941, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI145693145, Length=355, Percent_Identity=28.4507042253521, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1788107, Length=362, Percent_Identity=26.5193370165746, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1786810, Length=362, Percent_Identity=25.6906077348066, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790505, Length=128, Percent_Identity=31.25, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17558820, Length=363, Percent_Identity=27.8236914600551, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32563687, Length=372, Percent_Identity=30.1075268817204, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=378, Percent_Identity=27.2486772486773, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=371, Percent_Identity=26.6846361185984, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=364, Percent_Identity=25, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17559526, Length=372, Percent_Identity=23.6559139784946, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=360, Percent_Identity=25, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319264, Length=415, Percent_Identity=26.0240963855422, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323182, Length=396, Percent_Identity=24.7474747474747, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24581924, Length=356, Percent_Identity=25.2808988764045, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI18859661, Length=366, Percent_Identity=26.5027322404372, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24653035, Length=343, Percent_Identity=25.3644314868805, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19922652, Length=291, Percent_Identity=27.8350515463918, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21356947, Length=352, Percent_Identity=25.5681818181818, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 36729; Mature: 36729

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDGRDPALVVLGPPGDRESATLRAGLRVGEPVDDDVALVAATSGTTGAPKGAMLTAAAL
CCCCCCCEEEEECCCCCCCCHHHEECCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHH
RASATATHERLGGPGSWLLALPAHHIAGVQVLVRSLLAGSTPVELDVSRGFDVTQLPAAT
HHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHCCHHH
RALGTGRRYASLVAVQLAKALGDPAATAALAELDAVLLGGGPAPRPVLQAAAAAGITVVR
HHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHHHHH
SYGMSETAGGCVYDGVPLDGVRVRVSDGRIALGGATLAKGYRNPVDPDPFAEPGWFLTDD
HCCCCCCCCCEEECCCCCCCEEEEEECCEEEECCHHHHHCCCCCCCCCCCCCCCCEEECC
LGAVDDDGVLTVFGRADDAISTGGLTVLPQPVEAALCTHPAVADCAVFGLPDDRLGQRVV
CCCCCCCCEEEEEECCCCCCCCCCEEECCCHHHHHHHCCCCHHHEEEECCCCHHHHHHHH
AAIVLRDGRAAPSPDALRAHLSRTLDATAAPREVHVVAALPRRGIGKVDRAALVRRFAGG
HHHHEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCC
GEQ
CCC
>Mature Secondary Structure
MLDGRDPALVVLGPPGDRESATLRAGLRVGEPVDDDVALVAATSGTTGAPKGAMLTAAAL
CCCCCCCEEEEECCCCCCCCHHHEECCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHH
RASATATHERLGGPGSWLLALPAHHIAGVQVLVRSLLAGSTPVELDVSRGFDVTQLPAAT
HHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHCCHHH
RALGTGRRYASLVAVQLAKALGDPAATAALAELDAVLLGGGPAPRPVLQAAAAAGITVVR
HHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHHHHH
SYGMSETAGGCVYDGVPLDGVRVRVSDGRIALGGATLAKGYRNPVDPDPFAEPGWFLTDD
HCCCCCCCCCEEECCCCCCCEEEEEECCEEEECCHHHHHCCCCCCCCCCCCCCCCEEECC
LGAVDDDGVLTVFGRADDAISTGGLTVLPQPVEAALCTHPAVADCAVFGLPDDRLGQRVV
CCCCCCCCEEEEEECCCCCCCCCCEEECCCHHHHHHHCCCCHHHEEEECCCCHHHHHHHH
AAIVLRDGRAAPSPDALRAHLSRTLDATAAPREVHVVAALPRRGIGKVDRAALVRRFAGG
HHHHEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCC
GEQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]