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The map label for this gene is narK3_2

Identifier: 41409805

GI number: 41409805

Start: 4123368

End: 4124759

Strand: Reverse

Name: narK3_2

Synonym: MAP3707c

Alternate gene names: 41409805

Gene position: 4124759-4123368 (Counterclockwise)

Preceding gene: 41409806

Following gene: 41409804

Centisome position: 85.4

GC content: 68.61

Gene sequence:

>1392_bases
ATGGGCCGCGACCACCGCATCACGGACTGGAATCCGGAAGACGCAGCGGCCTGGGAGGCCGGCAACAAAAGGATCGCCCG
TCGCAACCTGCTGTGCACCATCGCCGGTGACCACGTGGCGTTTTCGATCTGGACCCTGTGGCCGGTGATGGCGCTGTTCA
TGCCCGCGGCCGTGTACGGCTTCTCCGCCGGCGACAAGCTGCTGCTGGGCGCGGTGGCCACCCTGGTCGGTGGCTGCGCC
CGGATCCCCTACACCCTGGGCATCGCGGCCTTCGGCGGCCGCAAATGGACGACTTTCTCGGCGGTCGTATTGCTGATCCC
CACCGTCGGCACCATCGTCTTGCTGGCCAATCCCGGTCTGCCGCTGTGGCTTTTCGTGCTGTGCGCCGCCCTGACCGGGC
TGGGCGGCGGCAACTATGCGGCGTCTCTGGCCAACGTCAACGCGTTCTATCCGCAGCGGCTCAAGGGCGCGGCCATGGCC
GTCAACGCCGGCGTCGCCAACCTGGGGGTGGCGGTGATCCAGCTGGTCGGCCTGCTGGTGCTGGCCACCGCCGGACACCA
GGCCCCGTACTGGGTGTGCGCGGTGTATCTGGTGTTCCTGGCCGCGGTGGCCATCGCCGCCGCGATGTTCATGGACGACA
TCAACCACGGCACCCAGCTGAGCACCATGCGCTCGATCTTGTTCGAACGCGACACCCATGTGATCTCGCTGCTCTACATC
GCCACCTTCGGCTCCTGGATCGGCTTCTCGTTCGCCTTCGGGCAGGTGATGCAGGTCAACTTCCTGGAGAACGGGGAAAG
CGCCAAGCATGCCGCGCTGCACGCCGCCCAGCTGGCCTTCATCGGCCCGCTGCTGGGCTCGGTGGCGCGGATCTACGGCG
GCCGGCTGGCCGACCGTGTCGGCGGCAGCCGCGTCACGCTGGGCGTGCTGGCCGCGATGACCCTGGCGGCCGGCCTGCTG
GTGATCGTCAGCACCGTCGACGACCAGCACGCGGGCGCGCATTCGGTCAGCATGATCGGCTACGTCGTCGGCTTCATGGT
GCTGTTCATCCTGTCCGGCATGGGCAACGGCTCGGTGTTCAAGCTGATCCCGTCGGTCTACGAGGCCCGCAGCCGCGGCC
GGGACACCAGCGAGGACGAGCGCCGCCAATGGGCGCGCGCCATGTCGGGGTCGCTGATCGGCATCTGCTCGGCGGTCGGG
GCGTTCGGCGGGGTGGCGATCAACTTGGCGCTGCACCAGTCCTACCTGAGCACCGGAACCGAGACGTCGGCGTACTGGAT
GTTCATGGCGTCCTACGTCGTCGCCGCCATCATGACCTGGCTGGTGTACGTGCGGCGGCCGGTCGCCGCGCCCGGGGTCT
CGCTGCCGGAAACCCAGGCCGCGCGGCTGTGA

Upstream 100 bases:

>100_bases
CGACGCCGTCCGATGGGCGCCATACCCGAACGCGCCCAACGGGTTTCGACGGCGAGCGGACGACAACGGCGTGTCTTCTT
CGAGGAAAGGTGACGATCGG

Downstream 100 bases:

>100_bases
GCGGACTCGCAACAGGGGCAATGCCACAGCAATTGCTCGGTAACGCAACGGAAACGTCCTAGGCATACACACTTCATATG
GCCCCGCAACTGGCCCCGCC

Product: NarK3_2

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MGRDHRITDWNPEDAAAWEAGNKRIARRNLLCTIAGDHVAFSIWTLWPVMALFMPAAVYGFSAGDKLLLGAVATLVGGCA
RIPYTLGIAAFGGRKWTTFSAVVLLIPTVGTIVLLANPGLPLWLFVLCAALTGLGGGNYAASLANVNAFYPQRLKGAAMA
VNAGVANLGVAVIQLVGLLVLATAGHQAPYWVCAVYLVFLAAVAIAAAMFMDDINHGTQLSTMRSILFERDTHVISLLYI
ATFGSWIGFSFAFGQVMQVNFLENGESAKHAALHAAQLAFIGPLLGSVARIYGGRLADRVGGSRVTLGVLAAMTLAAGLL
VIVSTVDDQHAGAHSVSMIGYVVGFMVLFILSGMGNGSVFKLIPSVYEARSRGRDTSEDERRQWARAMSGSLIGICSAVG
AFGGVAINLALHQSYLSTGTETSAYWMFMASYVVAAIMTWLVYVRRPVAAPGVSLPETQAARL

Sequences:

>Translated_463_residues
MGRDHRITDWNPEDAAAWEAGNKRIARRNLLCTIAGDHVAFSIWTLWPVMALFMPAAVYGFSAGDKLLLGAVATLVGGCA
RIPYTLGIAAFGGRKWTTFSAVVLLIPTVGTIVLLANPGLPLWLFVLCAALTGLGGGNYAASLANVNAFYPQRLKGAAMA
VNAGVANLGVAVIQLVGLLVLATAGHQAPYWVCAVYLVFLAAVAIAAAMFMDDINHGTQLSTMRSILFERDTHVISLLYI
ATFGSWIGFSFAFGQVMQVNFLENGESAKHAALHAAQLAFIGPLLGSVARIYGGRLADRVGGSRVTLGVLAAMTLAAGLL
VIVSTVDDQHAGAHSVSMIGYVVGFMVLFILSGMGNGSVFKLIPSVYEARSRGRDTSEDERRQWARAMSGSLIGICSAVG
AFGGVAINLALHQSYLSTGTETSAYWMFMASYVVAAIMTWLVYVRRPVAAPGVSLPETQAARL
>Mature_462_residues
GRDHRITDWNPEDAAAWEAGNKRIARRNLLCTIAGDHVAFSIWTLWPVMALFMPAAVYGFSAGDKLLLGAVATLVGGCAR
IPYTLGIAAFGGRKWTTFSAVVLLIPTVGTIVLLANPGLPLWLFVLCAALTGLGGGNYAASLANVNAFYPQRLKGAAMAV
NAGVANLGVAVIQLVGLLVLATAGHQAPYWVCAVYLVFLAAVAIAAAMFMDDINHGTQLSTMRSILFERDTHVISLLYIA
TFGSWIGFSFAFGQVMQVNFLENGESAKHAALHAAQLAFIGPLLGSVARIYGGRLADRVGGSRVTLGVLAAMTLAAGLLV
IVSTVDDQHAGAHSVSMIGYVVGFMVLFILSGMGNGSVFKLIPSVYEARSRGRDTSEDERRQWARAMSGSLIGICSAVGA
FGGVAINLALHQSYLSTGTETSAYWMFMASYVVAAIMTWLVYVRRPVAAPGVSLPETQAARL

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=461, Percent_Identity=33.8394793926247, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1787743, Length=462, Percent_Identity=32.9004329004329, Blast_Score=217, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48938; Mature: 48807

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRDHRITDWNPEDAAAWEAGNKRIARRNLLCTIAGDHVAFSIWTLWPVMALFMPAAVYG
CCCCCCCCCCCCCCHHHHHCCCHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHC
FSAGDKLLLGAVATLVGGCARIPYTLGIAAFGGRKWTTFSAVVLLIPTVGTIVLLANPGL
CCCCCHHHHHHHHHHHHHHHHCCHHHHHEECCCCCHHHHHHHHHHHHCCCEEEEEECCCC
PLWLFVLCAALTGLGGGNYAASLANVNAFYPQRLKGAAMAVNAGVANLGVAVIQLVGLLV
HHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHCCHHHEECCCHHHHHHHHHHHHHHHH
LATAGHQAPYWVCAVYLVFLAAVAIAAAMFMDDINHGTQLSTMRSILFERDTHVISLLYI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHH
ATFGSWIGFSFAFGQVMQVNFLENGESAKHAALHAAQLAFIGPLLGSVARIYGGRLADRV
HHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHC
GGSRVTLGVLAAMTLAAGLLVIVSTVDDQHAGAHSVSMIGYVVGFMVLFILSGMGNGSVF
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH
KLIPSVYEARSRGRDTSEDERRQWARAMSGSLIGICSAVGAFGGVAINLALHQSYLSTGT
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEHHHHHHHHHHCCC
ETSAYWMFMASYVVAAIMTWLVYVRRPVAAPGVSLPETQAARL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCC
>Mature Secondary Structure 
GRDHRITDWNPEDAAAWEAGNKRIARRNLLCTIAGDHVAFSIWTLWPVMALFMPAAVYG
CCCCCCCCCCCCCHHHHHCCCHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHC
FSAGDKLLLGAVATLVGGCARIPYTLGIAAFGGRKWTTFSAVVLLIPTVGTIVLLANPGL
CCCCCHHHHHHHHHHHHHHHHCCHHHHHEECCCCCHHHHHHHHHHHHCCCEEEEEECCCC
PLWLFVLCAALTGLGGGNYAASLANVNAFYPQRLKGAAMAVNAGVANLGVAVIQLVGLLV
HHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHCCHHHEECCCHHHHHHHHHHHHHHHH
LATAGHQAPYWVCAVYLVFLAAVAIAAAMFMDDINHGTQLSTMRSILFERDTHVISLLYI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHH
ATFGSWIGFSFAFGQVMQVNFLENGESAKHAALHAAQLAFIGPLLGSVARIYGGRLADRV
HHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHC
GGSRVTLGVLAAMTLAAGLLVIVSTVDDQHAGAHSVSMIGYVVGFMVLFILSGMGNGSVF
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH
KLIPSVYEARSRGRDTSEDERRQWARAMSGSLIGICSAVGAFGGVAINLALHQSYLSTGT
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEHHHHHHHHHHCCC
ETSAYWMFMASYVVAAIMTWLVYVRRPVAAPGVSLPETQAARL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]