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The map label for this gene is sdhA

Identifier: 41409796

GI number: 41409796

Start: 4113848

End: 4115791

Strand: Reverse

Name: sdhA

Synonym: MAP3698c

Alternate gene names: 41409796

Gene position: 4115791-4113848 (Counterclockwise)

Preceding gene: 41409797

Following gene: 41409795

Centisome position: 85.22

GC content: 68.26

Gene sequence:

>1944_bases
ATGGTTGAGGTCGAACGGCATGCCTACGACGTAGTCGTCATCGGCGCCGGCGGCGCGGGATTGCGCGCGGTCATCGAGGC
GCGGGAACGCGGCCTGCGCGTCGCGGTGGTGTGCAAGTCGTTGTTCGGCAAGGCGCACACCGTGATGGCCGAGGGCGGCT
GCGCGGCCTCGATGGGCAACACCAACCCGAAGGACAACTGGAAGACCCACTTCGGCGACACCATGCGCGGGGGCAAGTTC
CTCAACAACTGGCGCATGGCCGAGCTGCACGCCAAGGAGGCCCCGGACCGCGTCTGGGAGCTGGAAACCTATGGCGCGCT
CTTCGATCGGCTCAAGGACGGCAAGATCAGCCAGCGCAACTTCGGTGGGCACACCTACCCGCGGCTGGCGCACGTCGGTG
ACCGCACCGGCCTGGAGCTGATCCGCACCATGCAGCAGAAGATCGTCTCGCTGCAGCAGGAGGATTACGCCGAACTCGGC
GACTACGAGGCGCGCATCAAGGTGTTCGCCGAGTGCACGATCACCGAGCTGCTCAAGGACGGCGACGCGATCGCCGGCGC
GTTCGGCTATTGGCGCGAGAGCGGCCAGTTCGTGCTGTTCGAGGCGCCCGCGGTGGTGCTGGCCACCGGCGGCATCGGCA
AGTCGTTCAAGGTCACCTCGAACTCCTGGGAGTACACCGGCGACGGGCACGCCCTGGCGCTGCGGGCGGGCGCATCGCTG
ATCAACATGGAGTTCGTTCAGTTCCATCCGACGGGCATGGTGTGGCCGCCGAGTGTGAAAGGGATCCTGGTCACCGAGGG
TGTCCGCGGTGACGGCGGGGTGCTGAAGAACTCCGAGGGCAAGCGGTTCATGTTCGACTACATCCCCCCGGTGTTCAAGG
GCCAGTACGCCGAGACCGAGCAAGAAGCCGACCAGTGGCTCAAGGACAACGACTCGGCCCGCCGCACTCCCGACCTGCTG
CCGCGCGACGAGGTCGCGCGCGCCATCAACTCCGAGGTCAAGGCCGGCCGCGGCAGCCCGCACGGCGGCGTCTTCCTCGA
CATCGCGTCGCGGCTGACACCGGCGGAGATCAACCGCCGGCTGCCGTCGATGTACCACCAGTTCAAGGAGCTGGCCGGGG
TCGACATCACCAAGGAGCCGATGGAGGTCGGACCGACCTGCCACTACGTGATGGGCGGCGTCGAGGTGGACGCCGACACC
GGGGCGGCCACCGTCCCCGGCCTGTTCGCGGCCGGTGAGTGCTCCGGCGGCATGCACGGCTCCAACCGGCTGGGCGGCAA
CTCGCTGTCGGACCTGCTGGTGTTCGGCCGCCGGGCCGGGCTGGGCGCCGCCGACTACGTGCGCGCGCTGAGCAGCCGTC
CGACGATCGGCGAGAGCGCCGTCGAGGCGGCGGCGAAGCGGGCCCTGTCCCCCTTCGAGGCGCCGGCGGGCGGCGGCCCC
GGCGAGAACCCCTACACCCTGCAGCTCGAGCTGCAGCAGTCGATGAACGACCTGGTCGGCATCATCCGCAATGCCGACGA
GATCTCCGAGGCTTTGGCCCGGCTCGACAAGCTGCGGGAACGGTTCAAGAACCTGCACGTGGAGGGGCAGCGCCGCTACA
ACCCGGGCTGGAACCTGGCCATCGACCTGCGCAACATGCTGCTGGTCAGCGAATGCGTCGCCAAGGCCGCGCTGCAGCGC
ACCGAGAGCCGGGGCGGGCACACCCGCGACGACCACCCGTCGATGGACTCGTCGTGGCGCAAGCTGTTGCTGGTCTGCGA
GGCAGTCGCCGAGCCAGGCGACGTTGAAGCCGCGGTGATCCCCGACATCACCATCACGAAGAAAGAGCAGACGCCGATGC
GGCCCGATCTGCTGGAACTGTTCGACATCGCCGAGCTGGAGAAGTACTACACCGACGAGGAGCTCGCCGGGCATCCAGGG
CGGACACCCAGGACGGAGAAGTAA

Upstream 100 bases:

>100_bases
TTTCTACATCGCCCTGGTGGCCAGCGGCACCATCCCCGACCTGAGATTTGTTGGCTGACAAGCCATTTGACGAAGAATCA
GAATTAGCTAGCGAGGTTTC

Downstream 100 bases:

>100_bases
ATGACCTACAACGCGACGATGCGGGTGTGGCGCGGCGACGACGCCAACGGTGCGTTGCAGGACTTCACCGTCGAGGTCAA
CGAGGGTGAGGTCGTCCTCG

Product: succinate dehydrogenase flavoprotein subunit

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MVEVERHAYDVVVIGAGGAGLRAVIEARERGLRVAVVCKSLFGKAHTVMAEGGCAASMGNTNPKDNWKTHFGDTMRGGKF
LNNWRMAELHAKEAPDRVWELETYGALFDRLKDGKISQRNFGGHTYPRLAHVGDRTGLELIRTMQQKIVSLQQEDYAELG
DYEARIKVFAECTITELLKDGDAIAGAFGYWRESGQFVLFEAPAVVLATGGIGKSFKVTSNSWEYTGDGHALALRAGASL
INMEFVQFHPTGMVWPPSVKGILVTEGVRGDGGVLKNSEGKRFMFDYIPPVFKGQYAETEQEADQWLKDNDSARRTPDLL
PRDEVARAINSEVKAGRGSPHGGVFLDIASRLTPAEINRRLPSMYHQFKELAGVDITKEPMEVGPTCHYVMGGVEVDADT
GAATVPGLFAAGECSGGMHGSNRLGGNSLSDLLVFGRRAGLGAADYVRALSSRPTIGESAVEAAAKRALSPFEAPAGGGP
GENPYTLQLELQQSMNDLVGIIRNADEISEALARLDKLRERFKNLHVEGQRRYNPGWNLAIDLRNMLLVSECVAKAALQR
TESRGGHTRDDHPSMDSSWRKLLLVCEAVAEPGDVEAAVIPDITITKKEQTPMRPDLLELFDIAELEKYYTDEELAGHPG
RTPRTEK

Sequences:

>Translated_647_residues
MVEVERHAYDVVVIGAGGAGLRAVIEARERGLRVAVVCKSLFGKAHTVMAEGGCAASMGNTNPKDNWKTHFGDTMRGGKF
LNNWRMAELHAKEAPDRVWELETYGALFDRLKDGKISQRNFGGHTYPRLAHVGDRTGLELIRTMQQKIVSLQQEDYAELG
DYEARIKVFAECTITELLKDGDAIAGAFGYWRESGQFVLFEAPAVVLATGGIGKSFKVTSNSWEYTGDGHALALRAGASL
INMEFVQFHPTGMVWPPSVKGILVTEGVRGDGGVLKNSEGKRFMFDYIPPVFKGQYAETEQEADQWLKDNDSARRTPDLL
PRDEVARAINSEVKAGRGSPHGGVFLDIASRLTPAEINRRLPSMYHQFKELAGVDITKEPMEVGPTCHYVMGGVEVDADT
GAATVPGLFAAGECSGGMHGSNRLGGNSLSDLLVFGRRAGLGAADYVRALSSRPTIGESAVEAAAKRALSPFEAPAGGGP
GENPYTLQLELQQSMNDLVGIIRNADEISEALARLDKLRERFKNLHVEGQRRYNPGWNLAIDLRNMLLVSECVAKAALQR
TESRGGHTRDDHPSMDSSWRKLLLVCEAVAEPGDVEAAVIPDITITKKEQTPMRPDLLELFDIAELEKYYTDEELAGHPG
RTPRTEK
>Mature_647_residues
MVEVERHAYDVVVIGAGGAGLRAVIEARERGLRVAVVCKSLFGKAHTVMAEGGCAASMGNTNPKDNWKTHFGDTMRGGKF
LNNWRMAELHAKEAPDRVWELETYGALFDRLKDGKISQRNFGGHTYPRLAHVGDRTGLELIRTMQQKIVSLQQEDYAELG
DYEARIKVFAECTITELLKDGDAIAGAFGYWRESGQFVLFEAPAVVLATGGIGKSFKVTSNSWEYTGDGHALALRAGASL
INMEFVQFHPTGMVWPPSVKGILVTEGVRGDGGVLKNSEGKRFMFDYIPPVFKGQYAETEQEADQWLKDNDSARRTPDLL
PRDEVARAINSEVKAGRGSPHGGVFLDIASRLTPAEINRRLPSMYHQFKELAGVDITKEPMEVGPTCHYVMGGVEVDADT
GAATVPGLFAAGECSGGMHGSNRLGGNSLSDLLVFGRRAGLGAADYVRALSSRPTIGESAVEAAAKRALSPFEAPAGGGP
GENPYTLQLELQQSMNDLVGIIRNADEISEALARLDKLRERFKNLHVEGQRRYNPGWNLAIDLRNMLLVSECVAKAALQR
TESRGGHTRDDHPSMDSSWRKLLLVCEAVAEPGDVEAAVIPDITITKKEQTPMRPDLLELFDIAELEKYYTDEELAGHPG
RTPRTEK

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=569, Percent_Identity=33.3919156414763, Blast_Score=265, Evalue=7e-71,
Organism=Escherichia coli, GI1786942, Length=609, Percent_Identity=35.9605911330049, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI1790597, Length=544, Percent_Identity=35.1102941176471, Blast_Score=275, Evalue=5e-75,
Organism=Escherichia coli, GI1788928, Length=591, Percent_Identity=31.3028764805415, Blast_Score=225, Evalue=6e-60,
Organism=Caenorhabditis elegans, GI17550100, Length=655, Percent_Identity=34.8091603053435, Blast_Score=285, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI17505833, Length=590, Percent_Identity=35.2542372881356, Blast_Score=284, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI71986328, Length=435, Percent_Identity=27.5862068965517, Blast_Score=112, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6322416, Length=634, Percent_Identity=33.9116719242902, Blast_Score=297, Evalue=3e-81,
Organism=Saccharomyces cerevisiae, GI6322701, Length=636, Percent_Identity=33.4905660377358, Blast_Score=295, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6322511, Length=480, Percent_Identity=25.625, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320788, Length=488, Percent_Identity=27.0491803278689, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI17137288, Length=633, Percent_Identity=33.6492890995261, Blast_Score=281, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24655642, Length=633, Percent_Identity=33.6492890995261, Blast_Score=281, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24655647, Length=633, Percent_Identity=33.6492890995261, Blast_Score=281, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24663005, Length=586, Percent_Identity=33.9590443686007, Blast_Score=271, Evalue=1e-72,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1

Molecular weight: Translated: 70725; Mature: 70725

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00141 ASP_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEVERHAYDVVVIGAGGAGLRAVIEARERGLRVAVVCKSLFGKAHTVMAEGGCAASMGN
CCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEHHHHHHHHCCCEEEEECCCCEECCCC
TNPKDNWKTHFGDTMRGGKFLNNWRMAELHAKEAPDRVWELETYGALFDRLKDGKISQRN
CCCCCCCCHHCCCCCCCCHHHCCCEEHHHHHHCCCCHHEEHHHHHHHHHHHCCCCCCCCC
FGGHTYPRLAHVGDRTGLELIRTMQQKIVSLQQEDYAELGDYEARIKVFAECTITELLKD
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHC
GDAIAGAFGYWRESGQFVLFEAPAVVLATGGIGKSFKVTSNSWEYTGDGHALALRAGASL
CCHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCEEEEECCCCEECCCCCEEEEECCCCE
INMEFVQFHPTGMVWPPSVKGILVTEGVRGDGGVLKNSEGKRFMFDYIPPVFKGQYAETE
EEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCEECCCCCEEEHHCCCHHHCCCCCCCH
QEADQWLKDNDSARRTPDLLPRDEVARAINSEVKAGRGSPHGGVFLDIASRLTPAEINRR
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHCCCHHHHHHH
LPSMYHQFKELAGVDITKEPMEVGPTCHYVMGGVEVDADTGAATVPGLFAAGECSGGMHG
HHHHHHHHHHHHCCCCCCCHHHCCCCEEEEECCEEEECCCCCCCCCCCEECCCCCCCCCC
SNRLGGNSLSDLLVFGRRAGLGAADYVRALSSRPTIGESAVEAAAKRALSPFEAPAGGGP
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
GENPYTLQLELQQSMNDLVGIIRNADEISEALARLDKLRERFKNLHVEGQRRYNPGWNLA
CCCCEEEEEEHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEE
IDLRNMLLVSECVAKAALQRTESRGGHTRDDHPSMDSSWRKLLLVCEAVAEPGDVEAAVI
EHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEE
PDITITKKEQTPMRPDLLELFDIAELEKYYTDEELAGHPGRTPRTEK
CCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure
MVEVERHAYDVVVIGAGGAGLRAVIEARERGLRVAVVCKSLFGKAHTVMAEGGCAASMGN
CCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEHHHHHHHHCCCEEEEECCCCEECCCC
TNPKDNWKTHFGDTMRGGKFLNNWRMAELHAKEAPDRVWELETYGALFDRLKDGKISQRN
CCCCCCCCHHCCCCCCCCHHHCCCEEHHHHHHCCCCHHEEHHHHHHHHHHHCCCCCCCCC
FGGHTYPRLAHVGDRTGLELIRTMQQKIVSLQQEDYAELGDYEARIKVFAECTITELLKD
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHC
GDAIAGAFGYWRESGQFVLFEAPAVVLATGGIGKSFKVTSNSWEYTGDGHALALRAGASL
CCHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCEEEEECCCCEECCCCCEEEEECCCCE
INMEFVQFHPTGMVWPPSVKGILVTEGVRGDGGVLKNSEGKRFMFDYIPPVFKGQYAETE
EEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCEECCCCCEEEHHCCCHHHCCCCCCCH
QEADQWLKDNDSARRTPDLLPRDEVARAINSEVKAGRGSPHGGVFLDIASRLTPAEINRR
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHCCCHHHHHHH
LPSMYHQFKELAGVDITKEPMEVGPTCHYVMGGVEVDADTGAATVPGLFAAGECSGGMHG
HHHHHHHHHHHHCCCCCCCHHHCCCCEEEEECCEEEECCCCCCCCCCCEECCCCCCCCCC
SNRLGGNSLSDLLVFGRRAGLGAADYVRALSSRPTIGESAVEAAAKRALSPFEAPAGGGP
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
GENPYTLQLELQQSMNDLVGIIRNADEISEALARLDKLRERFKNLHVEGQRRYNPGWNLA
CCCCEEEEEEHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEE
IDLRNMLLVSECVAKAALQRTESRGGHTRDDHPSMDSSWRKLLLVCEAVAEPGDVEAAVI
EHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEE
PDITITKKEQTPMRPDLLELFDIAELEKYYTDEELAGHPGRTPRTEK
CCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.02 {succinate}} 0.4 {fumarate}} 0.3 {ferricyanide}} [C]

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]