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The map label for this gene is echA14

Identifier: 41408404

GI number: 41408404

Start: 2586109

End: 2586879

Strand: Direct

Name: echA14

Synonym: MAP2306

Alternate gene names: 41408404

Gene position: 2586109-2586879 (Clockwise)

Preceding gene: 41408403

Following gene: 41408413

Centisome position: 53.55

GC content: 72.63

Gene sequence:

>771_bases
ATGGCCCAGTCAAATCTCGTCCTGCTCGACATCGAGAACCACGTCGCGCTCATCACCGTCAACGATCCCGACCGGCGCAA
CGCGGTGACCGCCGAGATGTCGGGGCGGTTGCGCGAGGCCGTCGAGCGGGTCGAGGCCGACTCCGACGTGCACGCCGTGG
TGATCACCGGCGCCGGCAAGGCGTTTTGCGCCGGCGCCGACCTCAGCGCCCTGGGCGCCGCCGGCGGAGGCTCGGCCGAG
ACGGGGCTGCAGCAGCTTTACGACGGCTTCATGGCGATCGGGAGTTGCCGGCTGCCCACCATCGCCGCGGTCAACGGCGC
GGCCGTCGGCGCGGGCCTGAACCTGGCCCTGGCGGCCGACGTGCGCATCGCCGGGCCCGCCGCCCTGTTCGATCCGCGAT
TCCAGCAGCTGGGCCTGCACCCGGGCGGAGGCGCCACCTGGATGCTGCAGCGCGCGGTGGGCCCGCAGGTCGCCCGCGCG
GCCCTGTTGTTCGGCATGCGCTTCGACGCCGAGGCCGCGGTGCGCCATGGCCTGGCGCTCAGCGTCGCGGACGACCCGGT
GGCCGCGGCGCTCGAACTGGCCGCCGGCCCGGCGGCCGCCCCGCGCGAGGTGGTGCTGGCGACCAAAGCCACGATGCGCG
CCACCATCAGTCTCGGCTCGCTCGACAACGAACAGCATCAGCACGCCATGCGCACCGAGCTGGGCCCGCAGGCACATTCC
ATTCAATCACCGGAGTTCGCACAGCGATTGGCCGCCGCGCAACGCAAGTAG

Upstream 100 bases:

>100_bases
GGCCCGGCAAGGCCTAGCAGTACCCTTACCTCGCCCTGTCGCCGCGTCACTCCTGGCGACGAGCCGAGACCGCAGCCCAC
ACCCGAACCGGAGGTGCCCG

Downstream 100 bases:

>100_bases
GCTCACAAATCCAGCAGCGCGGTTTCCGGCGATTCGATCAAATCCCGCAGCTCGCAAACGAATCGAGCCGCCTGCGCGCC
GTCGGCGATGCGGTGGTCGA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MAQSNLVLLDIENHVALITVNDPDRRNAVTAEMSGRLREAVERVEADSDVHAVVITGAGKAFCAGADLSALGAAGGGSAE
TGLQQLYDGFMAIGSCRLPTIAAVNGAAVGAGLNLALAADVRIAGPAALFDPRFQQLGLHPGGGATWMLQRAVGPQVARA
ALLFGMRFDAEAAVRHGLALSVADDPVAAALELAAGPAAAPREVVLATKATMRATISLGSLDNEQHQHAMRTELGPQAHS
IQSPEFAQRLAAAQRK

Sequences:

>Translated_256_residues
MAQSNLVLLDIENHVALITVNDPDRRNAVTAEMSGRLREAVERVEADSDVHAVVITGAGKAFCAGADLSALGAAGGGSAE
TGLQQLYDGFMAIGSCRLPTIAAVNGAAVGAGLNLALAADVRIAGPAALFDPRFQQLGLHPGGGATWMLQRAVGPQVARA
ALLFGMRFDAEAAVRHGLALSVADDPVAAALELAAGPAAAPREVVLATKATMRATISLGSLDNEQHQHAMRTELGPQAHS
IQSPEFAQRLAAAQRK
>Mature_255_residues
AQSNLVLLDIENHVALITVNDPDRRNAVTAEMSGRLREAVERVEADSDVHAVVITGAGKAFCAGADLSALGAAGGGSAET
GLQQLYDGFMAIGSCRLPTIAAVNGAAVGAGLNLALAADVRIAGPAALFDPRFQQLGLHPGGGATWMLQRAVGPQVARAA
LLFGMRFDAEAAVRHGLALSVADDPVAAALELAAGPAAAPREVVLATKATMRATISLGSLDNEQHQHAMRTELGPQAHSI
QSPEFAQRLAAAQRK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=213, Percent_Identity=32.8638497652582, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI37594469, Length=190, Percent_Identity=30, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI37594471, Length=190, Percent_Identity=30, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI70995211, Length=167, Percent_Identity=31.1377245508982, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI213417737, Length=212, Percent_Identity=26.8867924528302, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI157694516, Length=212, Percent_Identity=26.8867924528302, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI260274832, Length=181, Percent_Identity=28.1767955801105, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI45643119, Length=181, Percent_Identity=28.1767955801105, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI260275230, Length=181, Percent_Identity=28.1767955801105, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI68989263, Length=215, Percent_Identity=29.3023255813954, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787660, Length=202, Percent_Identity=36.1386138613861, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787659, Length=190, Percent_Identity=35.7894736842105, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1788597, Length=171, Percent_Identity=31.5789473684211, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788682, Length=207, Percent_Identity=31.4009661835749, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI221142681, Length=162, Percent_Identity=34.5679012345679, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790281, Length=209, Percent_Identity=27.7511961722488, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=206, Percent_Identity=33.9805825242718, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=169, Percent_Identity=37.2781065088757, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17560910, Length=259, Percent_Identity=28.1853281853282, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=217, Percent_Identity=28.110599078341, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=181, Percent_Identity=32.0441988950276, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17540714, Length=182, Percent_Identity=27.4725274725275, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=181, Percent_Identity=33.1491712707182, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=216, Percent_Identity=32.4074074074074, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=195, Percent_Identity=26.1538461538462, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=142, Percent_Identity=29.5774647887324, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=142, Percent_Identity=29.5774647887324, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=142, Percent_Identity=29.5774647887324, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI20129971, Length=182, Percent_Identity=34.0659340659341, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24653477, Length=182, Percent_Identity=34.0659340659341, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19920382, Length=174, Percent_Identity=29.8850574712644, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571730, Length=193, Percent_Identity=28.4974093264249, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI28571729, Length=193, Percent_Identity=28.4974093264249, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24653139, Length=165, Percent_Identity=30.3030303030303, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24654903, Length=251, Percent_Identity=25.4980079681275, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24650670, Length=177, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21357171, Length=135, Percent_Identity=28.8888888888889, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 26291; Mature: 26160

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSNLVLLDIENHVALITVNDPDRRNAVTAEMSGRLREAVERVEADSDVHAVVITGAGK
CCCCCEEEEEECCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
AFCAGADLSALGAAGGGSAETGLQQLYDGFMAIGSCRLPTIAAVNGAAVGAGLNLALAAD
EEECCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEECCCCEEEEEEC
VRIAGPAALFDPRFQQLGLHPGGGATWMLQRAVGPQVARAALLFGMRFDAEAAVRHGLAL
EEECCCHHHCCCHHHHCCCCCCCCHHEEEHHHCCHHHHHHHHHHHCCCCHHHHHHCCEEE
SVADDPVAAALELAAGPAAAPREVVLATKATMRATISLGSLDNEQHQHAMRTELGPQAHS
EECCCHHHHHHHHHCCCCCCCCEEEEEEHHHEEEEEEECCCCCHHHHHHHHHHCCCCCCC
IQSPEFAQRLAAAQRK
CCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AQSNLVLLDIENHVALITVNDPDRRNAVTAEMSGRLREAVERVEADSDVHAVVITGAGK
CCCCEEEEEECCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
AFCAGADLSALGAAGGGSAETGLQQLYDGFMAIGSCRLPTIAAVNGAAVGAGLNLALAAD
EEECCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEECCCCEEEEEEC
VRIAGPAALFDPRFQQLGLHPGGGATWMLQRAVGPQVARAALLFGMRFDAEAAVRHGLAL
EEECCCHHHCCCHHHHCCCCCCCCHHEEEHHHCCHHHHHHHHHHHCCCCHHHHHHCCEEE
SVADDPVAAALELAAGPAAAPREVVLATKATMRATISLGSLDNEQHQHAMRTELGPQAHS
EECCCHHHHHHHHHCCCCCCCCEEEEEEHHHEEEEEEECCCCCHHHHHHHHHHCCCCCCC
IQSPEFAQRLAAAQRK
CCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]