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The map label for this gene is tsnR

Identifier: 41407453

GI number: 41407453

Start: 1455920

End: 1456711

Strand: Direct

Name: tsnR

Synonym: MAP1355

Alternate gene names: 41407453

Gene position: 1455920-1456711 (Clockwise)

Preceding gene: 41407452

Following gene: 41407455

Centisome position: 30.14

GC content: 75.38

Gene sequence:

>792_bases
GTGCTGACCGAACGTTCGGGCAGGGTCGCCGCGGCGGCCAAACTGCACCGCCACGTCGGCCGGCGCCGGGCCGCCCGGTT
CCTGGCCGAGGGCTCCAACCTGGTCGAGGCGGCCGCGGGCCGCGGCCTGGTCCGCGACGTCTTCGTCACCGAAGCCGCGC
AGCAGCGGTATGCGCCGCTGCTGGCGGCGCTGGACTGCCCGGTCCATCTGGTCACCGAGCGCGCCGCCAAGGCGCTCTCC
GACACCGTCACCCCGGCCGGGCTGGTCGCGGTGTGCGACCTGCCGGCCACCGGCCTGCGCGACGTGCTGGCCGGGTCGCC
GCGGCTCATCGCGGTCGCGGTCCAGATCAGCGAGCCGGGCAACGCCGGCACCCTGATTCGGCTGGCCGACGCCATGGGCG
CGGCCGGCGTGCTGCTGGCCGGCCACAGCGTCGACCCGTACAACGGCAAATGCCTGCGCGCATCGGCCGGCAGCATCTTC
TCCGTCCCGGTGGCCGCCGCGCCGGACCTGCGCGAGGCCGTCGCCGCGGTGCGCGAGGCCGGCCTGCAGCTGCTGGCCAC
CACCGTGGACGGCGAGGTGCGCCTGGATCGGGCGGGTGAACTGCTGGCCAAGCCGACAGCCTGGCTGTTCGGCCCCGAAT
CACACGGTCTGCCAAGCGCAGTCGCGGCGGCGGCCGATCACCGGGTGTGCATCCCGATGGCCGGCGGCGCGGAGAGCCTG
AACGTGGCCTCGGCCGCGGCGATCTGCCTCTACCAGAGCGCGCGGGCGCTGGAGCTGTTCACGCGGCCCTGA

Upstream 100 bases:

>100_bases
GGTCGACGTCGCCCGGGCGGCGCTGCCCGAGGACGTCAACGCCCCGTCGGACTCCGGAGAAGCGGCCTAACCGACCCGGC
GACGACGGGGACGCCGCAGG

Downstream 100 bases:

>100_bases
CCGGTCGTTTCGGCCGGCCCCGCCCGGCGCGCAGCCCAGCCAGCGACAACGTGGTGTGCACCAGCGATCCGGCGCGTTCC
ATCAGCGCCCGCGCGGGCTG

Product: TsnR

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MLTERSGRVAAAAKLHRHVGRRRAARFLAEGSNLVEAAAGRGLVRDVFVTEAAQQRYAPLLAALDCPVHLVTERAAKALS
DTVTPAGLVAVCDLPATGLRDVLAGSPRLIAVAVQISEPGNAGTLIRLADAMGAAGVLLAGHSVDPYNGKCLRASAGSIF
SVPVAAAPDLREAVAAVREAGLQLLATTVDGEVRLDRAGELLAKPTAWLFGPESHGLPSAVAAAADHRVCIPMAGGAESL
NVASAAAICLYQSARALELFTRP

Sequences:

>Translated_263_residues
MLTERSGRVAAAAKLHRHVGRRRAARFLAEGSNLVEAAAGRGLVRDVFVTEAAQQRYAPLLAALDCPVHLVTERAAKALS
DTVTPAGLVAVCDLPATGLRDVLAGSPRLIAVAVQISEPGNAGTLIRLADAMGAAGVLLAGHSVDPYNGKCLRASAGSIF
SVPVAAAPDLREAVAAVREAGLQLLATTVDGEVRLDRAGELLAKPTAWLFGPESHGLPSAVAAAADHRVCIPMAGGAESL
NVASAAAICLYQSARALELFTRP
>Mature_263_residues
MLTERSGRVAAAAKLHRHVGRRRAARFLAEGSNLVEAAAGRGLVRDVFVTEAAQQRYAPLLAALDCPVHLVTERAAKALS
DTVTPAGLVAVCDLPATGLRDVLAGSPRLIAVAVQISEPGNAGTLIRLADAMGAAGVLLAGHSVDPYNGKCLRASAGSIF
SVPVAAAPDLREAVAAVREAGLQLLATTVDGEVRLDRAGELLAKPTAWLFGPESHGLPSAVAAAADHRVCIPMAGGAESL
NVASAAAICLYQSARALELFTRP

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=154, Percent_Identity=35.0649350649351, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790623, Length=231, Percent_Identity=24.2424242424242, Blast_Score=63, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24666840, Length=272, Percent_Identity=26.8382352941176, Blast_Score=74, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 26970; Mature: 26970

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTERSGRVAAAAKLHRHVGRRRAARFLAEGSNLVEAAAGRGLVRDVFVTEAAQQRYAPL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LAALDCPVHLVTERAAKALSDTVTPAGLVAVCDLPATGLRDVLAGSPRLIAVAVQISEPG
HHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHCCCCEEEEEEEEECCCC
NAGTLIRLADAMGAAGVLLAGHSVDPYNGKCLRASAGSIFSVPVAAAPDLREAVAAVREA
CCCCEEEEHHHCCCCEEEEECCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHC
GLQLLATTVDGEVRLDRAGELLAKPTAWLFGPESHGLPSAVAAAADHRVCIPMAGGAESL
CCEEEEEECCCEEEECCCCHHHHCCCEEEECCCCCCCCHHHHHHCCCEEEEEECCCCCCC
NVASAAAICLYQSARALELFTRP
HHHHHHHHHHHHCCHHHHEECCC
>Mature Secondary Structure
MLTERSGRVAAAAKLHRHVGRRRAARFLAEGSNLVEAAAGRGLVRDVFVTEAAQQRYAPL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LAALDCPVHLVTERAAKALSDTVTPAGLVAVCDLPATGLRDVLAGSPRLIAVAVQISEPG
HHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHCCCCEEEEEEEEECCCC
NAGTLIRLADAMGAAGVLLAGHSVDPYNGKCLRASAGSIFSVPVAAAPDLREAVAAVREA
CCCCEEEEHHHCCCCEEEEECCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHC
GLQLLATTVDGEVRLDRAGELLAKPTAWLFGPESHGLPSAVAAAADHRVCIPMAGGAESL
CCEEEEEECCCEEEECCCCHHHHCCCEEEECCCCCCCCHHHHHHCCCEEEEEECCCCCCC
NVASAAAICLYQSARALELFTRP
HHHHHHHHHHHHCCHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]