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The map label for this gene is bioB

Identifier: 41407381

GI number: 41407381

Start: 1372266

End: 1373300

Strand: Direct

Name: bioB

Synonym: MAP1283

Alternate gene names: 41407381

Gene position: 1372266-1373300 (Clockwise)

Preceding gene: 41407375

Following gene: 41407382

Centisome position: 28.41

GC content: 70.05

Gene sequence:

>1035_bases
GTGACGCAAGCGGCGACGCGACCGACGGCCGAGATCGGCAACGAGGACATCCTGGCGGTGGCGCGGCGCCAGGTACTGGA
GGGCGGCCAGGGATTGAGCCGCGACCAGGTGCTGCAGGTGCTGCGGCTGCCCGACGACCGGCTCGACGACCTGCTGGCGC
TGGCCCACGAGGTGCGGATGCGTTGGTGCGGCCCGGAGGTCGAGGTCGAGGGCATCATCAGCCTCAAGACCGGCGGCTGC
CCCGAGGACTGCCACTTCTGCTCGCAGTCGGGGCTGTTCGCGTCCCCGGTGCGCAGCGCCCGGCTCGACATCCCCAGCCT
GGTCGAGGCGGCCAAGCAGACCGCCAAGTCCGGCGCCACCGAGTTCTGCATCGTGGCCGCCGTGCGCGGCCCCGACGAGC
GGTTGCTGGCCCAGGTCGCGGCCGGCATCGAGGCCGTCCGCAACGAGGTCGAGATCAACATCGCCTGCTCGCTGGGCATG
CTGACCGCCGAGCAGGTGGAGCAGCTCGCCGAGATGGGCGTACACCGCTACAACCACAACCTGGAGACGGCCCGGTCGTT
CTTCCCCAACGTGGTGACCACCCACACCTGGGAAGAGCGCTGGGACACCTTGTCGATGGTGCGCGACGCCGGCATGGAGG
TCTGCTGCGGCGGCATCCTCGGCATGGGCGAAACCCTCGAGCAGCGTGCGGAATTCGCCGCCGAACTGGCCGAGCTCGGC
CCCGACGAGGTGCCGCTGAACTTCCTCAACCCGCGCCCGGGCACCCCGTTCGGCGACCTGGAGGTGATGCCGGCGACCGA
GGCGCTGAAGTCGGTGGCCGCCTTCCGGCTCGCGTTGCCGCGCACCATGCTGCGGTTCGCCGGCGGCCGCGAGATCACCC
TCGGCGACCTGGGCGCCAAGAAGGGCATCCTGGGCGGCATCAACGCCGTGATCGTCGGCAACTACCTGACCACGCTGGGG
CGTCCGGCCGAATCCGACCTGGAATTGCTGGACGATCTGCAAATGCCCCTCAAGGGGCTCAATGCCAGCCTCTGA

Upstream 100 bases:

>100_bases
TCGGCCGTTACCCTACTTTGAACACCTTTTCGTTCAGGCTGCCATCGATGGCAGACGTGCCCGCACGCGCGGGCACCCGC
AAGAGGCAGGGGAGTACCTG

Downstream 100 bases:

>100_bases
GCCGGCCCGCACGCCGGTTTGTCGCCCGCGCGGCTAGGGTTGGAAACTTGTGGTTGGTGATCTGGGCGCCCTGGGCGAAG
TCGTCGGCGCCGGCGTCTAC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MTQAATRPTAEIGNEDILAVARRQVLEGGQGLSRDQVLQVLRLPDDRLDDLLALAHEVRMRWCGPEVEVEGIISLKTGGC
PEDCHFCSQSGLFASPVRSARLDIPSLVEAAKQTAKSGATEFCIVAAVRGPDERLLAQVAAGIEAVRNEVEINIACSLGM
LTAEQVEQLAEMGVHRYNHNLETARSFFPNVVTTHTWEERWDTLSMVRDAGMEVCCGGILGMGETLEQRAEFAAELAELG
PDEVPLNFLNPRPGTPFGDLEVMPATEALKSVAAFRLALPRTMLRFAGGREITLGDLGAKKGILGGINAVIVGNYLTTLG
RPAESDLELLDDLQMPLKGLNASL

Sequences:

>Translated_344_residues
MTQAATRPTAEIGNEDILAVARRQVLEGGQGLSRDQVLQVLRLPDDRLDDLLALAHEVRMRWCGPEVEVEGIISLKTGGC
PEDCHFCSQSGLFASPVRSARLDIPSLVEAAKQTAKSGATEFCIVAAVRGPDERLLAQVAAGIEAVRNEVEINIACSLGM
LTAEQVEQLAEMGVHRYNHNLETARSFFPNVVTTHTWEERWDTLSMVRDAGMEVCCGGILGMGETLEQRAEFAAELAELG
PDEVPLNFLNPRPGTPFGDLEVMPATEALKSVAAFRLALPRTMLRFAGGREITLGDLGAKKGILGGINAVIVGNYLTTLG
RPAESDLELLDDLQMPLKGLNASL
>Mature_343_residues
TQAATRPTAEIGNEDILAVARRQVLEGGQGLSRDQVLQVLRLPDDRLDDLLALAHEVRMRWCGPEVEVEGIISLKTGGCP
EDCHFCSQSGLFASPVRSARLDIPSLVEAAKQTAKSGATEFCIVAAVRGPDERLLAQVAAGIEAVRNEVEINIACSLGML
TAEQVEQLAEMGVHRYNHNLETARSFFPNVVTTHTWEERWDTLSMVRDAGMEVCCGGILGMGETLEQRAEFAAELAELGP
DEVPLNFLNPRPGTPFGDLEVMPATEALKSVAAFRLALPRTMLRFAGGREITLGDLGAKKGILGGINAVIVGNYLTTLGR
PAESDLELLDDLQMPLKGLNASL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=312, Percent_Identity=37.5, Blast_Score=199, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6321725, Length=320, Percent_Identity=27.5, Blast_Score=153, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 37084; Mature: 36953

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAATRPTAEIGNEDILAVARRQVLEGGQGLSRDQVLQVLRLPDDRLDDLLALAHEVRM
CCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
RWCGPEVEVEGIISLKTGGCPEDCHFCSQSGLFASPVRSARLDIPSLVEAAKQTAKSGAT
HHCCCCEEEEEEEEECCCCCCHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCC
EFCIVAAVRGPDERLLAQVAAGIEAVRNEVEINIACSLGMLTAEQVEQLAEMGVHRYNHN
CEEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHCCC
LETARSFFPNVVTTHTWEERWDTLSMVRDAGMEVCCGGILGMGETLEQRAEFAAELAELG
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
PDEVPLNFLNPRPGTPFGDLEVMPATEALKSVAAFRLALPRTMLRFAGGREITLGDLGAK
CCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
KGILGGINAVIVGNYLTTLGRPAESDLELLDDLQMPLKGLNASL
CCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TQAATRPTAEIGNEDILAVARRQVLEGGQGLSRDQVLQVLRLPDDRLDDLLALAHEVRM
CCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
RWCGPEVEVEGIISLKTGGCPEDCHFCSQSGLFASPVRSARLDIPSLVEAAKQTAKSGAT
HHCCCCEEEEEEEEECCCCCCHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCC
EFCIVAAVRGPDERLLAQVAAGIEAVRNEVEINIACSLGMLTAEQVEQLAEMGVHRYNHN
CEEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHCCC
LETARSFFPNVVTTHTWEERWDTLSMVRDAGMEVCCGGILGMGETLEQRAEFAAELAELG
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
PDEVPLNFLNPRPGTPFGDLEVMPATEALKSVAAFRLALPRTMLRFAGGREITLGDLGAK
CCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
KGILGGINAVIVGNYLTTLGRPAESDLELLDDLQMPLKGLNASL
CCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA