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The map label for this gene is aceF [H]

Identifier: 39997750

GI number: 39997750

Start: 2927243

End: 2928421

Strand: Direct

Name: aceF [H]

Synonym: GSU2656

Alternate gene names: 39997750

Gene position: 2927243-2928421 (Clockwise)

Preceding gene: 39997749

Following gene: 39997752

Centisome position: 76.75

GC content: 66.16

Gene sequence:

>1179_bases
ATGCCGTATGACTTCAAGCTCCCCGATCTGGGCGAAGGGATAACCGAGGCGGAACTGCGCCGCTGGCTCGTGAAGGAAGG
GGATACCGTAGCCGAGCACCAGCCGGTGGTGGAGGTGGAGACCGACAAGGCAGTGGTGGAGGTCCCCTCGCCCCGAGCCG
GGAGGGTGATAACGCGTGCCCGGCTGGAAGGGGAAACCGTCATGGTCGGAGAGACGCTCCTCACCATCGCTGAAGAGGAA
GCGACTCCCCCCGTGCGGAAACCCTCCGTGGGGATCGTGGGCGAACTCCCCGAGGCGGAGGAAGCAGTCGGGACACAGCA
GCCGGCCATCCTCGCCACCCCCCTGGTCAGGAAACTGGCCCGGGAACGGGGCATCGACCTGGCAACCGTGCGGGGAAGCG
GCCCGCGGGGGAGCATTACCCCCGAAGACGTGGCGGGCGCGGGCGCCCCGGCCCGGCCGGATGCCGGCGAGTTCGGGCCG
GCGGAGCGGATTCCCCTGCGGGGAGTGCGGCGGTCCATCGCCCGGAACGTCATGACCTCCCAGCGGAACACCGCCTTTGT
CACCGGCATGGAAGAGGCGGACATCACCGAACTGTGGCACCTGCGGGAACGGGAGCAGCAGGCGGTGGAACAGCGGGGCA
CCCACCTCACCTTCCTCCCCTTCTTCATCAAGGCCGTCCAGCACGCCCTGCGGGAGCATCCCTACCTGAATGCCGCCATT
GACGACGTGGCCGGAGAAATCATCCTCAAAAAGCACTATCACTTCGGCATTGCCGTGGAGACCCCCGACGGCCTCATGGT
GCCGGTAATCCGCAACGTGGACGCCAAGAGCATCATCGAACTGGCTTCGGAACTCCAGGAACTGGGCCGCAAGGCCCGGG
AGAGGACCATCACCCTGGACGAAATGCGGGGCAGCACCTTCACCCTCACCAACTTCGGCCACTTCGGGGGCGTGTTCGCC
ACCCCCGTCATCAACTGGCCCGACGTGGCCATCCTCGGTTTCGGCCGCATTGCCGACCGTCCATGGGTCCACGCCGGCCA
GATCGTCGTCCGCACGATCCTTCCCCTGTCCCTCACCTTCGACCACCGGGTTACCGACGGAGCCGACGCGGCCCAGTTCC
TGAGCAAGGTCGTCCGCTACCTGGAAGATCCGGCACTCCTGTTTATTGAAAGCATCTGA

Upstream 100 bases:

>100_bases
CGACGCTTGGAGGTTTTCGGAGCAGGAGCCCCGCCCCCGCCGGGCACCACCTACGCCTTACGCTTCATAGTGCAGTAACG
AGTTCTTCCGGGAGGAACCG

Downstream 100 bases:

>100_bases
GCAGACCGGGACAGTCCCGGCAAAAGGAAAGGCGGGAGATTCCTCTCCCGCCTTTCACATGCCTCATAGTGCGGCTTCCG
TTATCGCGGCGGTCCGAACA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MPYDFKLPDLGEGITEAELRRWLVKEGDTVAEHQPVVEVETDKAVVEVPSPRAGRVITRARLEGETVMVGETLLTIAEEE
ATPPVRKPSVGIVGELPEAEEAVGTQQPAILATPLVRKLARERGIDLATVRGSGPRGSITPEDVAGAGAPARPDAGEFGP
AERIPLRGVRRSIARNVMTSQRNTAFVTGMEEADITELWHLREREQQAVEQRGTHLTFLPFFIKAVQHALREHPYLNAAI
DDVAGEIILKKHYHFGIAVETPDGLMVPVIRNVDAKSIIELASELQELGRKARERTITLDEMRGSTFTLTNFGHFGGVFA
TPVINWPDVAILGFGRIADRPWVHAGQIVVRTILPLSLTFDHRVTDGADAAQFLSKVVRYLEDPALLFIESI

Sequences:

>Translated_392_residues
MPYDFKLPDLGEGITEAELRRWLVKEGDTVAEHQPVVEVETDKAVVEVPSPRAGRVITRARLEGETVMVGETLLTIAEEE
ATPPVRKPSVGIVGELPEAEEAVGTQQPAILATPLVRKLARERGIDLATVRGSGPRGSITPEDVAGAGAPARPDAGEFGP
AERIPLRGVRRSIARNVMTSQRNTAFVTGMEEADITELWHLREREQQAVEQRGTHLTFLPFFIKAVQHALREHPYLNAAI
DDVAGEIILKKHYHFGIAVETPDGLMVPVIRNVDAKSIIELASELQELGRKARERTITLDEMRGSTFTLTNFGHFGGVFA
TPVINWPDVAILGFGRIADRPWVHAGQIVVRTILPLSLTFDHRVTDGADAAQFLSKVVRYLEDPALLFIESI
>Mature_391_residues
PYDFKLPDLGEGITEAELRRWLVKEGDTVAEHQPVVEVETDKAVVEVPSPRAGRVITRARLEGETVMVGETLLTIAEEEA
TPPVRKPSVGIVGELPEAEEAVGTQQPAILATPLVRKLARERGIDLATVRGSGPRGSITPEDVAGAGAPARPDAGEFGPA
ERIPLRGVRRSIARNVMTSQRNTAFVTGMEEADITELWHLREREQQAVEQRGTHLTFLPFFIKAVQHALREHPYLNAAID
DVAGEIILKKHYHFGIAVETPDGLMVPVIRNVDAKSIIELASELQELGRKARERTITLDEMRGSTFTLTNFGHFGGVFAT
PVINWPDVAILGFGRIADRPWVHAGQIVVRTILPLSLTFDHRVTDGADAAQFLSKVVRYLEDPALLFIESI

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=420, Percent_Identity=34.2857142857143, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI19923748, Length=242, Percent_Identity=38.4297520661157, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI31711992, Length=433, Percent_Identity=28.6374133949192, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI203098816, Length=455, Percent_Identity=25.4945054945055, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI203098753, Length=455, Percent_Identity=25.4945054945055, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI260898739, Length=135, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786305, Length=420, Percent_Identity=33.5714285714286, Blast_Score=214, Evalue=9e-57,
Organism=Escherichia coli, GI1786946, Length=414, Percent_Identity=30.6763285024155, Blast_Score=211, Evalue=7e-56,
Organism=Caenorhabditis elegans, GI17537937, Length=416, Percent_Identity=31.9711538461538, Blast_Score=212, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI25146366, Length=416, Percent_Identity=31.25, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17560088, Length=433, Percent_Identity=29.7921478060046, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17538894, Length=230, Percent_Identity=30.4347826086957, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=402, Percent_Identity=29.8507462686567, Blast_Score=189, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6324258, Length=447, Percent_Identity=24.3847874720358, Blast_Score=120, Evalue=4e-28,
Organism=Drosophila melanogaster, GI18859875, Length=423, Percent_Identity=31.9148936170213, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=35.6828193832599, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 43061; Mature: 42930

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYDFKLPDLGEGITEAELRRWLVKEGDTVAEHQPVVEVETDKAVVEVPSPRAGRVITRA
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCEEEECCCEEEECCCCCCCCEEEEE
RLEGETVMVGETLLTIAEEEATPPVRKPSVGIVGELPEAEEAVGTQQPAILATPLVRKLA
ECCCCEEEECHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHCCCCCCHHHHHHHHHHH
RERGIDLATVRGSGPRGSITPEDVAGAGAPARPDAGEFGPAERIPLRGVRRSIARNVMTS
HHCCCEEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
QRNTAFVTGMEEADITELWHLREREQQAVEQRGTHLTFLPFFIKAVQHALREHPYLNAAI
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCHHHH
DDVAGEIILKKHYHFGIAVETPDGLMVPVIRNVDAKSIIELASELQELGRKARERTITLD
HHHHHHHEEEEECEEEEEEECCCCEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEHH
EMRGSTFTLTNFGHFGGVFATPVINWPDVAILGFGRIADRPWVHAGQIVVRTILPLSLTF
HCCCCEEEEECCCCCCCCHHCCCCCCCCEEEEECCHHCCCCCCCHHHHHHHHHHHHEEEE
DHRVTDGADAAQFLSKVVRYLEDPALLFIESI
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure 
PYDFKLPDLGEGITEAELRRWLVKEGDTVAEHQPVVEVETDKAVVEVPSPRAGRVITRA
CCCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCEEEECCCEEEECCCCCCCCEEEEE
RLEGETVMVGETLLTIAEEEATPPVRKPSVGIVGELPEAEEAVGTQQPAILATPLVRKLA
ECCCCEEEECHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHCCCCCCHHHHHHHHHHH
RERGIDLATVRGSGPRGSITPEDVAGAGAPARPDAGEFGPAERIPLRGVRRSIARNVMTS
HHCCCEEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
QRNTAFVTGMEEADITELWHLREREQQAVEQRGTHLTFLPFFIKAVQHALREHPYLNAAI
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCHHHH
DDVAGEIILKKHYHFGIAVETPDGLMVPVIRNVDAKSIIELASELQELGRKARERTITLD
HHHHHHHEEEEECEEEEEEECCCCEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEHH
EMRGSTFTLTNFGHFGGVFATPVINWPDVAILGFGRIADRPWVHAGQIVVRTILPLSLTF
HCCCCEEEEECCCCCCCCHHCCCCCCCCEEEEECCHHCCCCCCCHHHHHHHHHHHHEEEE
DHRVTDGADAAQFLSKVVRYLEDPALLFIESI
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA