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The map label for this gene is istB [H]

Identifier: 39997689

GI number: 39997689

Start: 2859387

End: 2860181

Strand: Direct

Name: istB [H]

Synonym: GSU2594

Alternate gene names: 39997689

Gene position: 2859387-2860181 (Clockwise)

Preceding gene: 39997688

Following gene: 39997690

Centisome position: 74.97

GC content: 58.24

Gene sequence:

>795_bases
ATGAGCGACATCCAGCACCAGCGTATGGTCACGCTCTGCGATGATCTGAAGTTTCTGTCGGTGGCCGATGTCTACACCGA
CCTTGCAGATGCCGCGGCAAAGCAGGAATCCTCCTACATCGACTACCTTGAACAGGTACTCAAGGCCGAGAACGACGTCC
GACAGGGACGCTCGCGCCACACAATGGCAAAGCTCGCCGGTTTTCCCGCGATCAAGACTCTCGAGGATTATGACTTTGAG
TTCGCCACCGGCGCCCCGAAACAGCGAATTCTGGACCTGTCGGCGATGGCATTCCTCGAGCGCCGGGAGAATGTAATCCT
GCTGGGTCCCAGCGGCACCGGAAAGACTCACCTCGCCATCGCGCTCGGCTACCGCGCTACTCAGTGCGGCGTGAAGGTAC
GCTTCATCTCCGCCGCCGACCTGATGCTGCAACTTGAAAGCGCCCAGCGGCAGGGGCGGTACAAGGAAGTAATGCGGCGC
AGTGTCCTGGGGCCGAGACTGCTCATCATCGATGAGATCGGCTACCTCCCGTTCAGCGAAACGCAGGCGAATCTGTTCTT
TCAGGTGATCGCCAAAAGGTACGAAACAGGTTCCGTCATCCTCACTTCGAACCTGAGCTTCGGGGAATGGGAACAAGCCT
TCGGCGGCAATACGGCACTCACATCAGCTATGCTCGACCGACTGCTGCACCACGCCCATGTCATTCAGATCAGAGGTGAC
AGCTACCGGCTGAAAGAGAAGCGACGCGCAGGTATTCTCGGCCAGCAACTGCCGACACCTCAGATCGATGACTAA

Upstream 100 bases:

>100_bases
AGCCGGACAAGCCGCAAGTCATTGCTGCCATGCCGTGGCCGGTTATTCCCCTTCAGCGCCCCGCCTCCTTCTACGATCAG
ATCCTTCAGGAGGGGCGGCT

Downstream 100 bases:

>100_bases
TCCAGGAAGGTGGGTCATTTTTCAATTTCCGATCAACCGGTAAAGTGGGTCAATTTTCGATTGCCGTTGACAGCCCATGG
TCAATTGATCTACGGAGGCT

Product: transposase/IS protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSDIQHQRMVTLCDDLKFLSVADVYTDLADAAAKQESSYIDYLEQVLKAENDVRQGRSRHTMAKLAGFPAIKTLEDYDFE
FATGAPKQRILDLSAMAFLERRENVILLGPSGTGKTHLAIALGYRATQCGVKVRFISAADLMLQLESAQRQGRYKEVMRR
SVLGPRLLIIDEIGYLPFSETQANLFFQVIAKRYETGSVILTSNLSFGEWEQAFGGNTALTSAMLDRLLHHAHVIQIRGD
SYRLKEKRRAGILGQQLPTPQIDD

Sequences:

>Translated_264_residues
MSDIQHQRMVTLCDDLKFLSVADVYTDLADAAAKQESSYIDYLEQVLKAENDVRQGRSRHTMAKLAGFPAIKTLEDYDFE
FATGAPKQRILDLSAMAFLERRENVILLGPSGTGKTHLAIALGYRATQCGVKVRFISAADLMLQLESAQRQGRYKEVMRR
SVLGPRLLIIDEIGYLPFSETQANLFFQVIAKRYETGSVILTSNLSFGEWEQAFGGNTALTSAMLDRLLHHAHVIQIRGD
SYRLKEKRRAGILGQQLPTPQIDD
>Mature_263_residues
SDIQHQRMVTLCDDLKFLSVADVYTDLADAAAKQESSYIDYLEQVLKAENDVRQGRSRHTMAKLAGFPAIKTLEDYDFEF
ATGAPKQRILDLSAMAFLERRENVILLGPSGTGKTHLAIALGYRATQCGVKVRFISAADLMLQLESAQRQGRYKEVMRRS
VLGPRLLIIDEIGYLPFSETQANLFFQVIAKRYETGSVILTSNLSFGEWEQAFGGNTALTSAMLDRLLHHAHVIQIRGDS
YRLKEKRRAGILGQQLPTPQIDD

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 29627; Mature: 29496

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIQHQRMVTLCDDLKFLSVADVYTDLADAAAKQESSYIDYLEQVLKAENDVRQGRSRH
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
TMAKLAGFPAIKTLEDYDFEFATGAPKQRILDLSAMAFLERRENVILLGPSGTGKTHLAI
HHHHHHCCCCHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEE
ALGYRATQCGVKVRFISAADLMLQLESAQRQGRYKEVMRRSVLGPRLLIIDEIGYLPFSE
EECCEEHHCCEEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCCCC
TQANLFFQVIAKRYETGSVILTSNLSFGEWEQAFGGNTALTSAMLDRLLHHAHVIQIRGD
HHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHEEEEECCC
SYRLKEKRRAGILGQQLPTPQIDD
CHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDIQHQRMVTLCDDLKFLSVADVYTDLADAAAKQESSYIDYLEQVLKAENDVRQGRSRH
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
TMAKLAGFPAIKTLEDYDFEFATGAPKQRILDLSAMAFLERRENVILLGPSGTGKTHLAI
HHHHHHCCCCHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEE
ALGYRATQCGVKVRFISAADLMLQLESAQRQGRYKEVMRRSVLGPRLLIIDEIGYLPFSE
EECCEEHHCCEEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCCCC
TQANLFFQVIAKRYETGSVILTSNLSFGEWEQAFGGNTALTSAMLDRLLHHAHVIQIRGD
HHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHEEEEECCC
SYRLKEKRRAGILGQQLPTPQIDD
CHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]