Click here to switch to the map view.

The map label for this gene is lpdA-2 [H]

Identifier: 39997683

GI number: 39997683

Start: 2851313

End: 2852671

Strand: Direct

Name: lpdA-2 [H]

Synonym: GSU2588

Alternate gene names: 39997683

Gene position: 2851313-2852671 (Clockwise)

Preceding gene: 39997682

Following gene: 39997684

Centisome position: 74.76

GC content: 56.95

Gene sequence:

>1359_bases
ATGCAAACGTTTGATGTTGTTGTTATCGGCGGCGGTCCCGGAGGCATGACCGCAGGGATGATGCTGAAGCAGGCTGGCAA
ATCGGTCGCGATCATCCAGGAAAATCACGACAGTTTCGGCGGGGTCTGTCTCAACCGTGGCTGCATGCCGACCAAATCCA
TGCTAAAAGCGGCCAAGGTCTATCGCGATGCCCAAAACAGCGAGAAATACGGCCTGGACCTATCCGTGAACCCTGTCGAT
TTAACACGCCTGCGCGCCGTTGCCGACGCAGATCTGAACATGCTGCGTCATATGGTCCAGGGAAAACTGACCGATGCGCG
CATTGCCGTCTTCCGCGGCAAAGGCTCTTTCCTGTCCGAACATGAGCTACAGATCTGCCAAGCAGATGGCAGTTCCGAAC
AAATCCGCGGCGAAAAAATTATCATTGCGACCGGTTCGGTCCCCGCCGAACTTCCCTGTGCCCCCTTTGATGGGCACTCC
ATCCTCTCCAGTGACCAGATTCTGAAAAACACGGATCTCCCGCACAAGCTGCTGATTATCGGCGGCGGGGCGATAGGCTG
CGAGTTTGCAACCCTGTACAACACCTTCGGCAGCAGAGTTACCTTGGTTGAAGCCATGGATAGCCTGCTGCCACGGGAAG
ACAAGGAAGCCGGGAAAACGCTGCAGTCCACCTTTGAACAGCAGGGCATCACGGTGAAAACAGGCGCGGCCATCAAAAGC
ATTTCGGTCGAGGCTGGAACGGTCCATGTCCATTATGACGGCTCCTGCGCAACCGAAGAATTCGACAAGGTACTGGTCGG
CATCGGCCGCACAGCGAACATTGCCGGGCTGAACCTTGACGCTGCCGGAGTAGCGACCGAACAGGGCGCTGTCAAGGTTA
ACGAGATGATGCAGACCACCGTTCCCCACATCTACGCGCTGGGCGACGTGATCGGTGGCATGACCCTGGCCCATGCGGCA
GAAAAAGAAGGGTACCTGCTTGCCCAGAATCTTATTCAGGGCACTCGCCACCCCCTCGACCATCGTGCTGTCCCGCGAGT
TGTGTTCTGTCACCCCGAAGTGGCGGCAGTAGGAACACATGAAGCCAGGGCCGGCATTAAGGCCTTCACCATGCCACAGG
CCCCCAATGGCCGCGCCGTGGTGGACAAAGTCGCGCCGGCCTTCGTGAAGCTGTTTATCGAGGAGGATACCTCTCAAATC
GCCGGGGCAATCATCATCGGAGAAGGGGCAACAGAAATGATCCACGAGATGGCAGTGGCGGTCGAAAACCGTCTGACCCT
GGAGCAGATAGGGAAAACGGTCCATGCCCATCCGACTCATTCCAAAAATGTTCTGCTAGCCGTCCAGCACTTCAATTAA

Upstream 100 bases:

>100_bases
ACATGTCGCCAGAGGACCTGGCAACGTTCACCCGACTGCTCGATGTCGCTACAAAGGCCTTGGAGAACCCGGAAACAGAG
TAATCAACAGGAGAATCTCA

Downstream 100 bases:

>100_bases
TTCAACGATTGAACTCGTTATAATTGTTACCAAATAACCCGCCTCACATCAGTTATGATCTCAAGCGGCTCTCCGGCTTC
AGCATGGAGAGCCGCTACGG

Product: alpha keto acid dehydrogenase complex, E3 component, lipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MQTFDVVVIGGGPGGMTAGMMLKQAGKSVAIIQENHDSFGGVCLNRGCMPTKSMLKAAKVYRDAQNSEKYGLDLSVNPVD
LTRLRAVADADLNMLRHMVQGKLTDARIAVFRGKGSFLSEHELQICQADGSSEQIRGEKIIIATGSVPAELPCAPFDGHS
ILSSDQILKNTDLPHKLLIIGGGAIGCEFATLYNTFGSRVTLVEAMDSLLPREDKEAGKTLQSTFEQQGITVKTGAAIKS
ISVEAGTVHVHYDGSCATEEFDKVLVGIGRTANIAGLNLDAAGVATEQGAVKVNEMMQTTVPHIYALGDVIGGMTLAHAA
EKEGYLLAQNLIQGTRHPLDHRAVPRVVFCHPEVAAVGTHEARAGIKAFTMPQAPNGRAVVDKVAPAFVKLFIEEDTSQI
AGAIIIGEGATEMIHEMAVAVENRLTLEQIGKTVHAHPTHSKNVLLAVQHFN

Sequences:

>Translated_452_residues
MQTFDVVVIGGGPGGMTAGMMLKQAGKSVAIIQENHDSFGGVCLNRGCMPTKSMLKAAKVYRDAQNSEKYGLDLSVNPVD
LTRLRAVADADLNMLRHMVQGKLTDARIAVFRGKGSFLSEHELQICQADGSSEQIRGEKIIIATGSVPAELPCAPFDGHS
ILSSDQILKNTDLPHKLLIIGGGAIGCEFATLYNTFGSRVTLVEAMDSLLPREDKEAGKTLQSTFEQQGITVKTGAAIKS
ISVEAGTVHVHYDGSCATEEFDKVLVGIGRTANIAGLNLDAAGVATEQGAVKVNEMMQTTVPHIYALGDVIGGMTLAHAA
EKEGYLLAQNLIQGTRHPLDHRAVPRVVFCHPEVAAVGTHEARAGIKAFTMPQAPNGRAVVDKVAPAFVKLFIEEDTSQI
AGAIIIGEGATEMIHEMAVAVENRLTLEQIGKTVHAHPTHSKNVLLAVQHFN
>Mature_452_residues
MQTFDVVVIGGGPGGMTAGMMLKQAGKSVAIIQENHDSFGGVCLNRGCMPTKSMLKAAKVYRDAQNSEKYGLDLSVNPVD
LTRLRAVADADLNMLRHMVQGKLTDARIAVFRGKGSFLSEHELQICQADGSSEQIRGEKIIIATGSVPAELPCAPFDGHS
ILSSDQILKNTDLPHKLLIIGGGAIGCEFATLYNTFGSRVTLVEAMDSLLPREDKEAGKTLQSTFEQQGITVKTGAAIKS
ISVEAGTVHVHYDGSCATEEFDKVLVGIGRTANIAGLNLDAAGVATEQGAVKVNEMMQTTVPHIYALGDVIGGMTLAHAA
EKEGYLLAQNLIQGTRHPLDHRAVPRVVFCHPEVAAVGTHEARAGIKAFTMPQAPNGRAVVDKVAPAFVKLFIEEDTSQI
AGAIIIGEGATEMIHEMAVAVENRLTLEQIGKTVHAHPTHSKNVLLAVQHFN

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=457, Percent_Identity=33.0415754923414, Blast_Score=222, Evalue=7e-58,
Organism=Homo sapiens, GI50301238, Length=461, Percent_Identity=27.5488069414317, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI33519430, Length=430, Percent_Identity=26.9767441860465, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI33519428, Length=430, Percent_Identity=26.9767441860465, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI33519426, Length=430, Percent_Identity=26.9767441860465, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI22035672, Length=450, Percent_Identity=28.8888888888889, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI148277071, Length=430, Percent_Identity=26.9767441860465, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI148277065, Length=430, Percent_Identity=26.9767441860465, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI291045266, Length=424, Percent_Identity=25.9433962264151, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI291045268, Length=415, Percent_Identity=25.0602409638554, Blast_Score=99, Evalue=9e-21,
Organism=Escherichia coli, GI1786307, Length=457, Percent_Identity=32.8227571115974, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI87082354, Length=450, Percent_Identity=31.5555555555556, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI87081717, Length=456, Percent_Identity=30.4824561403509, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1789915, Length=432, Percent_Identity=28.4722222222222, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI32565766, Length=458, Percent_Identity=33.4061135371179, Blast_Score=218, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=26.5263157894737, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI71983429, Length=457, Percent_Identity=27.3522975929978, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71983419, Length=457, Percent_Identity=27.3522975929978, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=391, Percent_Identity=25.0639386189258, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=461, Percent_Identity=31.6702819956616, Blast_Score=202, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6325240, Length=472, Percent_Identity=27.5423728813559, Blast_Score=176, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6325166, Length=430, Percent_Identity=24.8837209302326, Blast_Score=146, Evalue=5e-36,
Organism=Drosophila melanogaster, GI21358499, Length=452, Percent_Identity=34.5132743362832, Blast_Score=245, Evalue=6e-65,
Organism=Drosophila melanogaster, GI17737741, Length=463, Percent_Identity=27.8617710583153, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24640549, Length=465, Percent_Identity=27.5268817204301, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640553, Length=462, Percent_Identity=27.7056277056277, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24640551, Length=462, Percent_Identity=27.7056277056277, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48081; Mature: 48081

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTFDVVVIGGGPGGMTAGMMLKQAGKSVAIIQENHDSFGGVCLNRGCMPTKSMLKAAKV
CCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCEEECCCCCCHHHHHHHHHH
YRDAQNSEKYGLDLSVNPVDLTRLRAVADADLNMLRHMVQGKLTDARIAVFRGKGSFLSE
HHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCC
HELQICQADGSSEQIRGEKIIIATGSVPAELPCAPFDGHSILSSDQILKNTDLPHKLLII
CCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEEEE
GGGAIGCEFATLYNTFGSRVTLVEAMDSLLPREDKEAGKTLQSTFEQQGITVKTGAAIKS
ECCCHHHHHHHHHHHHCCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCEEEE
ISVEAGTVHVHYDGSCATEEFDKVLVGIGRTANIAGLNLDAAGVATEQGAVKVNEMMQTT
EEEEEEEEEEEECCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCEEHHHHHHHH
VPHIYALGDVIGGMTLAHAAEKEGYLLAQNLIQGTRHPLDHRAVPRVVFCHPEVAAVGTH
HHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCEECCCH
EARAGIKAFTMPQAPNGRAVVDKVAPAFVKLFIEEDTSQIAGAIIIGEGATEMIHEMAVA
HHHCCCEEEECCCCCCCCCHHHHHHHHHHEEEHHCCHHHHCCEEEECCCHHHHHHHHHHH
VENRLTLEQIGKTVHAHPTHSKNVLLAVQHFN
HHCCCCHHHHCCEEECCCCCCCCEEEEEEECC
>Mature Secondary Structure
MQTFDVVVIGGGPGGMTAGMMLKQAGKSVAIIQENHDSFGGVCLNRGCMPTKSMLKAAKV
CCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCEEECCCCCCHHHHHHHHHH
YRDAQNSEKYGLDLSVNPVDLTRLRAVADADLNMLRHMVQGKLTDARIAVFRGKGSFLSE
HHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCC
HELQICQADGSSEQIRGEKIIIATGSVPAELPCAPFDGHSILSSDQILKNTDLPHKLLII
CCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEEEE
GGGAIGCEFATLYNTFGSRVTLVEAMDSLLPREDKEAGKTLQSTFEQQGITVKTGAAIKS
ECCCHHHHHHHHHHHHCCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCEEEE
ISVEAGTVHVHYDGSCATEEFDKVLVGIGRTANIAGLNLDAAGVATEQGAVKVNEMMQTT
EEEEEEEEEEEECCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCEEHHHHHHHH
VPHIYALGDVIGGMTLAHAAEKEGYLLAQNLIQGTRHPLDHRAVPRVVFCHPEVAAVGTH
HHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCEECCCH
EARAGIKAFTMPQAPNGRAVVDKVAPAFVKLFIEEDTSQIAGAIIIGEGATEMIHEMAVA
HHHCCCEEEECCCCCCCCCHHHHHHHHHHEEEHHCCHHHHCCEEEECCCHHHHHHHHHHH
VENRLTLEQIGKTVHAHPTHSKNVLLAVQHFN
HHCCCCHHHHCCEEECCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]