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The map label for this gene is mdeA [H]

Identifier: 39997520

GI number: 39997520

Start: 2657210

End: 2658493

Strand: Direct

Name: mdeA [H]

Synonym: GSU2425

Alternate gene names: 39997520

Gene position: 2657210-2658493 (Clockwise)

Preceding gene: 39997519

Following gene: 39997524

Centisome position: 69.67

GC content: 65.5

Gene sequence:

>1284_bases
ATGAGCAGTTCGTGGAAGATCGAGACCCAGGCCATCCAGGGGGGCTATACTCCCAAATCGGGCGACCCCCGCGTCGTCAC
CATCTGCCAGAGCACCACCTTCAAGTATGACAGCGCGGACCACGTGACGAAACTCTTCGACCTGGAGGAGGCCGGCTACT
TCTATACCCGCATCGGCAACCCCACCACCGACGCCTTCGAGCAGAAGCTGGCCATGATGGAAGGGGGCGTCGGCGCCCTG
GCCACCTCGGCGGGCCAGGCGGCCACCACCCTTTCCATCTTCAACATCTGCCAGGCGGGCCAGCACTTCGTGACCGCCAG
CACCCTCTACGGCGGCACCTACAACCTCTTCGCCTGCACCCTGCCCAAGATGGGGATCGAGGTGACCTTCGTGGACCCGG
AAGCCCCGGCCGACGAGATCCTGGCCGCCTTCCGCCCCAACACCAAGGCGCTCTTTGCCGAGACCATCGGCAATCCGGGG
CTGAACGTGCTGGACTTCGAGAAATTTTCCGCCGTCGCCAAAGAAAAAGGGGTGCCCCTCATCATCGACAGCACCTTCGC
CACCCCCTACCTCTGCCGCCCCTTCGAGCACGGGGCCAACGTGGTGGTCCACTCGGCCACCAAGTACATCGACGGCCTCG
CCACCAGCGTCGGCGGCATCATCGTGGACGGGGGCAACTTCGACTGGGGCAACGGCCGCTTCCCGGAGCTGACCGAGCCC
GACGCCTCCTATCATGGGCTCCAGTACGTGAAGACCTTCGGCCGCATGGCCTACATCATCAAGGCGCGGGTCCAGCTCAT
GCGCGACCTGGGCACGACGCCGAGCCCCTTCAACGCCTTCCTCTTCACCCAGGGGCTCCAGACCCTGCCGCTCCGGATGC
AGCGCCACAGCGACAACGCCCTGGCCCTGGGCCGGTTCCTGGAAAGCCATCCCTCGGTTAGCTGGGTCAAGTATCCGGGC
CTTCCCAGCCACGCCAGCCATGACCGGGCGAAAAAGTACCTGCCCCTGGGGTGCAGCGGCGTCCTCACCTTCGGCATCAA
GGGAGGTGCCGAGGCGGGCAAGAAGTTCATGGAGGCGTGCAAACTGGTGGCGCTGGTGGTCCACGTGGGGGACGCCCGCA
GCTGCGTGCTTCACCCGGCCAGCACCACCCACCGCCAACTGACCCCCGAGCAGCAGGCTGCGTCGGGCGTCACCCCGGAC
CTGATCCGGCTTTCCGTCGGCATCGAGCACATCGACGACATCATCGCCGATGTGGATCAGGCCCTGGCCGCAAGCCAGAA
ATAA

Upstream 100 bases:

>100_bases
ACTCCACTGATCCTTCGGCGGCACCTTGCTATTTCCCCGTTTCCCGAACGGCGTTTCTCTGGTATCATCGCGGCCTCACC
ATCGTTGAAAGGAGACCGCC

Downstream 100 bases:

>100_bases
CGGACGCTGCTCCACGACGCACAAAAGCCTGCCGGATCTCCGGCAGGCTTTTTCGTGTGTGCTGATCGCGGCCTGATGTG
AAAGACGATCAGTATTCCGT

Product: O-acetyl-L-homoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSSSWKIETQAIQGGYTPKSGDPRVVTICQSTTFKYDSADHVTKLFDLEEAGYFYTRIGNPTTDAFEQKLAMMEGGVGAL
ATSAGQAATTLSIFNICQAGQHFVTASTLYGGTYNLFACTLPKMGIEVTFVDPEAPADEILAAFRPNTKALFAETIGNPG
LNVLDFEKFSAVAKEKGVPLIIDSTFATPYLCRPFEHGANVVVHSATKYIDGLATSVGGIIVDGGNFDWGNGRFPELTEP
DASYHGLQYVKTFGRMAYIIKARVQLMRDLGTTPSPFNAFLFTQGLQTLPLRMQRHSDNALALGRFLESHPSVSWVKYPG
LPSHASHDRAKKYLPLGCSGVLTFGIKGGAEAGKKFMEACKLVALVVHVGDARSCVLHPASTTHRQLTPEQQAASGVTPD
LIRLSVGIEHIDDIIADVDQALAASQK

Sequences:

>Translated_427_residues
MSSSWKIETQAIQGGYTPKSGDPRVVTICQSTTFKYDSADHVTKLFDLEEAGYFYTRIGNPTTDAFEQKLAMMEGGVGAL
ATSAGQAATTLSIFNICQAGQHFVTASTLYGGTYNLFACTLPKMGIEVTFVDPEAPADEILAAFRPNTKALFAETIGNPG
LNVLDFEKFSAVAKEKGVPLIIDSTFATPYLCRPFEHGANVVVHSATKYIDGLATSVGGIIVDGGNFDWGNGRFPELTEP
DASYHGLQYVKTFGRMAYIIKARVQLMRDLGTTPSPFNAFLFTQGLQTLPLRMQRHSDNALALGRFLESHPSVSWVKYPG
LPSHASHDRAKKYLPLGCSGVLTFGIKGGAEAGKKFMEACKLVALVVHVGDARSCVLHPASTTHRQLTPEQQAASGVTPD
LIRLSVGIEHIDDIIADVDQALAASQK
>Mature_426_residues
SSSWKIETQAIQGGYTPKSGDPRVVTICQSTTFKYDSADHVTKLFDLEEAGYFYTRIGNPTTDAFEQKLAMMEGGVGALA
TSAGQAATTLSIFNICQAGQHFVTASTLYGGTYNLFACTLPKMGIEVTFVDPEAPADEILAAFRPNTKALFAETIGNPGL
NVLDFEKFSAVAKEKGVPLIIDSTFATPYLCRPFEHGANVVVHSATKYIDGLATSVGGIIVDGGNFDWGNGRFPELTEPD
ASYHGLQYVKTFGRMAYIIKARVQLMRDLGTTPSPFNAFLFTQGLQTLPLRMQRHSDNALALGRFLESHPSVSWVKYPGL
PSHASHDRAKKYLPLGCSGVLTFGIKGGAEAGKKFMEACKLVALVVHVGDARSCVLHPASTTHRQLTPEQQAASGVTPDL
IRLSVGIEHIDDIIADVDQALAASQK

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=421, Percent_Identity=33.4916864608076, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI299473759, Length=421, Percent_Identity=30.8788598574822, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI25453487, Length=420, Percent_Identity=30.2380952380952, Blast_Score=161, Evalue=1e-39,
Organism=Escherichia coli, GI1790375, Length=379, Percent_Identity=30.0791556728232, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1789383, Length=427, Percent_Identity=26.9320843091335, Blast_Score=139, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71980708, Length=406, Percent_Identity=33.9901477832512, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17538055, Length=420, Percent_Identity=35, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17560056, Length=419, Percent_Identity=34.8448687350835, Blast_Score=204, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6323333, Length=412, Percent_Identity=46.8446601941748, Blast_Score=357, Evalue=2e-99,
Organism=Saccharomyces cerevisiae, GI6319307, Length=428, Percent_Identity=33.1775700934579, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6321170, Length=330, Percent_Identity=28.1818181818182, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321254, Length=445, Percent_Identity=23.8202247191011, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19922554, Length=424, Percent_Identity=33.2547169811321, Blast_Score=215, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45989; Mature: 45858

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSWKIETQAIQGGYTPKSGDPRVVTICQSTTFKYDSADHVTKLFDLEEAGYFYTRIGN
CCCCCEEEEEEECCCCCCCCCCCCEEEEECCCEECCCCHHHHHHHHHHHHCCEEEEECCC
PTTDAFEQKLAMMEGGVGALATSAGQAATTLSIFNICQAGQHFVTASTLYGGTYNLFACT
CCHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEE
LPKMGIEVTFVDPEAPADEILAAFRPNTKALFAETIGNPGLNVLDFEKFSAVAKEKGVPL
CCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCE
IIDSTFATPYLCRPFEHGANVVVHSATKYIDGLATSVGGIIVDGGNFDWGNGRFPELTEP
EEECCCCCCCEECCHHCCCCEEEECHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCC
DASYHGLQYVKTFGRMAYIIKARVQLMRDLGTTPSPFNAFLFTQGLQTLPLRMQRHSDNA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHCHHHHCCCCCH
LALGRFLESHPSVSWVKYPGLPSHASHDRAKKYLPLGCSGVLTFGIKGGAEAGKKFMEAC
HHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHH
KLVALVVHVGDARSCVLHPASTTHRQLTPEQQAASGVTPDLIRLSVGIEHIDDIIADVDQ
HHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHH
ALAASQK
HHHCCCC
>Mature Secondary Structure 
SSSWKIETQAIQGGYTPKSGDPRVVTICQSTTFKYDSADHVTKLFDLEEAGYFYTRIGN
CCCCEEEEEEECCCCCCCCCCCCEEEEECCCEECCCCHHHHHHHHHHHHCCEEEEECCC
PTTDAFEQKLAMMEGGVGALATSAGQAATTLSIFNICQAGQHFVTASTLYGGTYNLFACT
CCHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEE
LPKMGIEVTFVDPEAPADEILAAFRPNTKALFAETIGNPGLNVLDFEKFSAVAKEKGVPL
CCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCE
IIDSTFATPYLCRPFEHGANVVVHSATKYIDGLATSVGGIIVDGGNFDWGNGRFPELTEP
EEECCCCCCCEECCHHCCCCEEEECHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCC
DASYHGLQYVKTFGRMAYIIKARVQLMRDLGTTPSPFNAFLFTQGLQTLPLRMQRHSDNA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHCHHHHCCCCCH
LALGRFLESHPSVSWVKYPGLPSHASHDRAKKYLPLGCSGVLTFGIKGGAEAGKKFMEAC
HHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHH
KLVALVVHVGDARSCVLHPASTTHRQLTPEQQAASGVTPDLIRLSVGIEHIDDIIADVDQ
HHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHH
ALAASQK
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]