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The map label for this gene is tesA [H]

Identifier: 39997510

GI number: 39997510

Start: 2649504

End: 2650163

Strand: Direct

Name: tesA [H]

Synonym: GSU2415

Alternate gene names: 39997510

Gene position: 2649504-2650163 (Clockwise)

Preceding gene: 39997509

Following gene: 39997511

Centisome position: 69.47

GC content: 67.88

Gene sequence:

>660_bases
ATGGCGGTTATTGCGCTGACCGCCCTGGCCGGGTGCGACCGCAAGGAGGCTGCCCGGCCCGCGGCTCCGGCGCAGCAGGA
GGCCCGTGCCACGGCAGGGACCATCGTGGCGGTGGGGGACAGCCTCACCGCCGGCTACGGCCTCGACGAACACGATGCCT
ACCCGGCCCAACTGGAGCGGCTGCTGCGGGAGGCCGGTCGCCCCTGGCGGGTGGTGAATGCGGGAATCAGCGGCGAGACG
AGCAGCGGCGCCCTGTCGCGCGTGAACTGGGTCCTAACCCTGAAGCCGGATATCGTTATCCTGGTCACCGGCGCCAACGA
CGGCCTGCGGGGGATCGACCCGAAGGTGACGGAAAAGAATCTCGACGAGATGGTCCGCACGCTGAAGGAGCGCAACGTCA
CCGTGGTCCTGGGCGGGATGAAGATGGTCGCGAACCTGGGCCGGGACTACACCCGCGCCTTTGCCGCCGTCTACCCGGCC
GTGGCGAAGCGCCACGACCTGATCCTGATTCCCTTTTTCCTGGAAGGGGTTGCAGGGCGCCCCGACCTGAACCAGGCCGA
CGGCATCCACCCCACGGCCGAGGGATACCGAATCGTGTCCGATACGGTACTTCCCTACGTGATCAAGGCCATCGAGCAGA
CTCCGCCATCTGGCGGCTGA

Upstream 100 bases:

>100_bases
AGGAAAAAGCCGGTGGTCTTTCTGCGGGAGCAGGCCGAGGAGTAACGGGGCCGTCGGGACCGGAATACGGAGGTATACCG
TGAATCTCAGGAAAAGTCTG

Downstream 100 bases:

>100_bases
AATCAAATGGCGTGTCACATTCGACATTAACCATTACTACATATAATTGCAAAGAAAACATCCTCCACACCCCCCATTCG
AACCAGCCGAAAATTCGGAC

Product: lipase/acylhydrolase

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MAVIALTALAGCDRKEAARPAAPAQQEARATAGTIVAVGDSLTAGYGLDEHDAYPAQLERLLREAGRPWRVVNAGISGET
SSGALSRVNWVLTLKPDIVILVTGANDGLRGIDPKVTEKNLDEMVRTLKERNVTVVLGGMKMVANLGRDYTRAFAAVYPA
VAKRHDLILIPFFLEGVAGRPDLNQADGIHPTAEGYRIVSDTVLPYVIKAIEQTPPSGG

Sequences:

>Translated_219_residues
MAVIALTALAGCDRKEAARPAAPAQQEARATAGTIVAVGDSLTAGYGLDEHDAYPAQLERLLREAGRPWRVVNAGISGET
SSGALSRVNWVLTLKPDIVILVTGANDGLRGIDPKVTEKNLDEMVRTLKERNVTVVLGGMKMVANLGRDYTRAFAAVYPA
VAKRHDLILIPFFLEGVAGRPDLNQADGIHPTAEGYRIVSDTVLPYVIKAIEQTPPSGG
>Mature_218_residues
AVIALTALAGCDRKEAARPAAPAQQEARATAGTIVAVGDSLTAGYGLDEHDAYPAQLERLLREAGRPWRVVNAGISGETS
SGALSRVNWVLTLKPDIVILVTGANDGLRGIDPKVTEKNLDEMVRTLKERNVTVVLGGMKMVANLGRDYTRAFAAVYPAV
AKRHDLILIPFFLEGVAGRPDLNQADGIHPTAEGYRIVSDTVLPYVIKAIEQTPPSGG

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=175, Percent_Identity=39.4285714285714, Blast_Score=128, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 23294; Mature: 23163

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01098 LIPASE_GDSL_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVIALTALAGCDRKEAARPAAPAQQEARATAGTIVAVGDSLTAGYGLDEHDAYPAQLER
CEEEEEEHHHCCCHHHHCCCCCCCHHHHHHHCCEEEEECCCCCCCCCCCCCCCCHHHHHH
LLREAGRPWRVVNAGISGETSSGALSRVNWVLTLKPDIVILVTGANDGLRGIDPKVTEKN
HHHHCCCCEEEEECCCCCCCCCCCCEEEEEEEEECCCEEEEEECCCCCCCCCCCCCCHHH
LDEMVRTLKERNVTVVLGGMKMVANLGRDYTRAFAAVYPAVAKRHDLILIPFFLEGVAGR
HHHHHHHHHHCCEEEEECCHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCC
PDLNQADGIHPTAEGYRIVSDTVLPYVIKAIEQTPPSGG
CCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AVIALTALAGCDRKEAARPAAPAQQEARATAGTIVAVGDSLTAGYGLDEHDAYPAQLER
EEEEEEHHHCCCHHHHCCCCCCCHHHHHHHCCEEEEECCCCCCCCCCCCCCCCHHHHHH
LLREAGRPWRVVNAGISGETSSGALSRVNWVLTLKPDIVILVTGANDGLRGIDPKVTEKN
HHHHCCCCEEEEECCCCCCCCCCCCEEEEEEEEECCCEEEEEECCCCCCCCCCCCCCHHH
LDEMVRTLKERNVTVVLGGMKMVANLGRDYTRAFAAVYPAVAKRHDLILIPFFLEGVAGR
HHHHHHHHHHCCEEEEECCHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCC
PDLNQADGIHPTAEGYRIVSDTVLPYVIKAIEQTPPSGG
CCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]