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The map label for this gene is nhaA

Identifier: 39997401

GI number: 39997401

Start: 2521446

End: 2522594

Strand: Direct

Name: nhaA

Synonym: GSU2303

Alternate gene names: 39997401

Gene position: 2521446-2522594 (Clockwise)

Preceding gene: 39997400

Following gene: 39997402

Centisome position: 66.11

GC content: 60.75

Gene sequence:

>1149_bases
TTGAGAAAAAGGATCAACCTGCTGCGTGAATTTTCGGTGCCTCTGATCGCCGGTGTCGTGGTGGCCCTGCTCTGGGCCAA
CCTCGACCCGGCCGGCTACCACTCTTTCATAGAACAGCCCTTCTTCGGTGGGATGAGCTTCCACTTCGTGGTGAATGAGC
TGTTCATGGTCCTTTTTTTCGGAATAGCGGCAGTTGAAATAACTCAAAGCTGCCTGCCGGGCGGCGATCTGAATCCGTTG
AGAAAGGCGGTCAATCCGCTTCTGGCGACTCTTGGAGGGGTCGTCGGCCCGGTGCTCGTCTACCTGGGACTCAACGCCAT
CATCGGTGGTCCGGAACTGACGCGCGGCTGGGGTATCCCCACCGCCACCGATATCGCGCTGGCATGGCTGGTAGCCCGGC
TCGTCTTCGGAGCCGGGCATCCCGCGGTATCGTTTCTGCTTCTGCTGGCGGTAGCGGACGATGCCATAGGACTTGCCATC
ATTGCCGTATTCTATCCCGACCCGAACCACCCGACCGAGCCGATCTGGCTTTTCCTGACGGTCGCCGGCATGGTTGTCGC
CTATATCCTCAGGAGCCTGAAATCAAGAAGCTACTGGCCCTATGTAGTCGTGGGAGGCGGACTTGCATGGACCGGCCTGT
TCAAGGCCCACTTGCACCCAGCCCTCGCCCTCGTCTTCATCATTCCCTTCCTGCCGCATCCAAGCCGGGAAACGGCTCAC
CTGTTCGAGGCCGATCCGCGCGACACCTCGACCCTGGCCCGGTTCGAGCATGATTGGAAGATCGTCGTCGACTTCGGTCT
CTTCATGTTCGGCCTTGCCAATGCGGGAGTCGGTTTTTCGTCCGTGGGGGCGGCAACCTGGCTCGTGCTGGCATCGCTCG
TAATCGGCAAGACGCTCGGCATCTTTGCCATGGGATACGTTGGCAGGGCCCTCGGATTCCCCCTGCCGCAGCAGGTCGGC
GCCAAGGAACTGGCCATGACGGGGCTGGTGGCGGGCATCGGCCTGACCGTGGCCCTGTTTGTGGCCGGTGTGGCATTTGT
TGAACCGGACATCCAGGGGTCGGCCAAAATGGGGGCCCTTTTGTCGGGAGGTGTCTCCATTGTGGCGATCATGCTCGGAA
GAGCGCTGAACGTGAGACGAATCCCCTGA

Upstream 100 bases:

>100_bases
ACCTGGCGGGAATCGACGAGCACCATCGGACGAGAATGGCGGGGCGCAGGCTCTGCAATGCCGCATAGCAGTCGTGCATG
GCTGACTGAAAGGATAGGAT

Downstream 100 bases:

>100_bases
GCTGCCCGGCTAATTGCATCTTGACAAATGTACTGCCCTGTTTAAGATGCGAATGACACACGATCATCCGCGAAAGACAC
CGATCCATGTACCGTCACGG

Product: Na+/H+ antiporter family protein

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MRKRINLLREFSVPLIAGVVVALLWANLDPAGYHSFIEQPFFGGMSFHFVVNELFMVLFFGIAAVEITQSCLPGGDLNPL
RKAVNPLLATLGGVVGPVLVYLGLNAIIGGPELTRGWGIPTATDIALAWLVARLVFGAGHPAVSFLLLLAVADDAIGLAI
IAVFYPDPNHPTEPIWLFLTVAGMVVAYILRSLKSRSYWPYVVVGGGLAWTGLFKAHLHPALALVFIIPFLPHPSRETAH
LFEADPRDTSTLARFEHDWKIVVDFGLFMFGLANAGVGFSSVGAATWLVLASLVIGKTLGIFAMGYVGRALGFPLPQQVG
AKELAMTGLVAGIGLTVALFVAGVAFVEPDIQGSAKMGALLSGGVSIVAIMLGRALNVRRIP

Sequences:

>Translated_382_residues
MRKRINLLREFSVPLIAGVVVALLWANLDPAGYHSFIEQPFFGGMSFHFVVNELFMVLFFGIAAVEITQSCLPGGDLNPL
RKAVNPLLATLGGVVGPVLVYLGLNAIIGGPELTRGWGIPTATDIALAWLVARLVFGAGHPAVSFLLLLAVADDAIGLAI
IAVFYPDPNHPTEPIWLFLTVAGMVVAYILRSLKSRSYWPYVVVGGGLAWTGLFKAHLHPALALVFIIPFLPHPSRETAH
LFEADPRDTSTLARFEHDWKIVVDFGLFMFGLANAGVGFSSVGAATWLVLASLVIGKTLGIFAMGYVGRALGFPLPQQVG
AKELAMTGLVAGIGLTVALFVAGVAFVEPDIQGSAKMGALLSGGVSIVAIMLGRALNVRRIP
>Mature_382_residues
MRKRINLLREFSVPLIAGVVVALLWANLDPAGYHSFIEQPFFGGMSFHFVVNELFMVLFFGIAAVEITQSCLPGGDLNPL
RKAVNPLLATLGGVVGPVLVYLGLNAIIGGPELTRGWGIPTATDIALAWLVARLVFGAGHPAVSFLLLLAVADDAIGLAI
IAVFYPDPNHPTEPIWLFLTVAGMVVAYILRSLKSRSYWPYVVVGGGLAWTGLFKAHLHPALALVFIIPFLPHPSRETAH
LFEADPRDTSTLARFEHDWKIVVDFGLFMFGLANAGVGFSSVGAATWLVLASLVIGKTLGIFAMGYVGRALGFPLPQQVG
AKELAMTGLVAGIGLTVALFVAGVAFVEPDIQGSAKMGALLSGGVSIVAIMLGRALNVRRIP

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3004

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaA Na(+)/H(+) (TC 2.A.33) antiporter family

Homologues:

Organism=Escherichia coli, GI1786201, Length=370, Percent_Identity=31.8918918918919, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAA_GEOSL (Q74AP9)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_953352.1
- HSSP:   P13738
- ProteinModelPortal:   Q74AP9
- SMR:   Q74AP9
- GeneID:   2687269
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU2303
- NMPDR:   fig|243231.1.peg.2290
- TIGR:   GSU2303
- HOGENOM:   HBG665619
- OMA:   VFGAGHP
- ProtClustDB:   CLSK828801
- BioCyc:   GSUL243231:GSU_2303-MONOMER
- HAMAP:   MF_01844
- InterPro:   IPR023171
- InterPro:   IPR004670

Pfam domain/function: PF06965 Na_H_antiport_1

EC number: NA

Molecular weight: Translated: 40572; Mature: 40572

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xb9db85c)-; HASH(0x102f76f0)-; HASH(0x10358648)-; HASH(0x106c9c80)-; HASH(0xb9c5c24)-; HASH(0x102f8cf0)-; HASH(0x104ae6ec)-; HASH(0x106c9c74)-; HASH(0x106f6764)-; HASH(0xebc6370)-; HASH(0xfe2fe10)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKRINLLREFSVPLIAGVVVALLWANLDPAGYHSFIEQPFFGGMSFHFVVNELFMVLFF
CCHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
GIAAVEITQSCLPGGDLNPLRKAVNPLLATLGGVVGPVLVYLGLNAIIGGPELTRGWGIP
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
TATDIALAWLVARLVFGAGHPAVSFLLLLAVADDAIGLAIIAVFYPDPNHPTEPIWLFLT
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHCEEEEEEEECCCCCCCCHHHHHHH
VAGMVVAYILRSLKSRSYWPYVVVGGGLAWTGLFKAHLHPALALVFIIPFLPHPSRETAH
HHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
LFEADPRDTSTLARFEHDWKIVVDFGLFMFGLANAGVGFSSVGAATWLVLASLVIGKTLG
HHCCCCCCHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
IFAMGYVGRALGFPLPQQVGAKELAMTGLVAGIGLTVALFVAGVAFVEPDIQGSAKMGAL
HHHHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LSGGVSIVAIMLGRALNVRRIP
HHCHHHHHHHHHHCCCCCCCCH
>Mature Secondary Structure
MRKRINLLREFSVPLIAGVVVALLWANLDPAGYHSFIEQPFFGGMSFHFVVNELFMVLFF
CCHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
GIAAVEITQSCLPGGDLNPLRKAVNPLLATLGGVVGPVLVYLGLNAIIGGPELTRGWGIP
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
TATDIALAWLVARLVFGAGHPAVSFLLLLAVADDAIGLAIIAVFYPDPNHPTEPIWLFLT
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHCEEEEEEEECCCCCCCCHHHHHHH
VAGMVVAYILRSLKSRSYWPYVVVGGGLAWTGLFKAHLHPALALVFIIPFLPHPSRETAH
HHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
LFEADPRDTSTLARFEHDWKIVVDFGLFMFGLANAGVGFSSVGAATWLVLASLVIGKTLG
HHCCCCCCHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
IFAMGYVGRALGFPLPQQVGAKELAMTGLVAGIGLTVALFVAGVAFVEPDIQGSAKMGAL
HHHHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LSGGVSIVAIMLGRALNVRRIP
HHCHHHHHHHHHHCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + 2 Proton [Periplasm] = Na (I) [Periplasm] + 2 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA