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The map label for this gene is pleD [H]

Identifier: 39997317

GI number: 39997317

Start: 2434536

End: 2434907

Strand: Reverse

Name: pleD [H]

Synonym: GSU2219

Alternate gene names: 39997317

Gene position: 2434907-2434536 (Counterclockwise)

Preceding gene: 39997318

Following gene: 39997316

Centisome position: 63.84

GC content: 55.65

Gene sequence:

>372_bases
ATGGAAAAGAAAAGGATTCTGATCGTCGAAGATGAAGAGAGTCTGCTCAAACTTGAAAGCATTCTGCTGTCTTCCCGCGG
TTACGCCGTGGCAGGGGTTACCGACGGCAAGTCCGCCCTTGACTATATTGCAGCCACACCTCCCGACCTGATCATTCTCG
ATATCATGTTGCCGGGAATGGATGGCTTCGAGGTCTGCCAGATGATCAAGGCCGACCCGTCAACCCGCCACATCCCGGTG
GTTATGCTCACTGCCAAGAAAAGCACCCAGGACCGTGACAAGGGGCTGGAGGCCGGTGCTTCCGCTTACGTGACCAAGCC
GTTCAAGTCGGCCCGGATCATCGAGATCATTCAGGGACTTCTGGCTGCCTGA

Upstream 100 bases:

>100_bases
GTCAACGGCATAGGCCGCCATGACGGCGGAATAATCATACTGCTTCAGCTGGAAAAGGTGCTCGATTCAGCATTGATGTA
ATACCCAGGAGGGTATGCCG

Downstream 100 bases:

>100_bases
CGCTGCCGGCCGCCCTTCGCATACGTATCAGACCCACGCTCCCGAAGGATACGCATGGAAACCGACATCCAGGAAATACA
GCTCGCCTGCTTCCGCCTCG

Product: response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 123; Mature: 123

Protein sequence:

>123_residues
MEKKRILIVEDEESLLKLESILLSSRGYAVAGVTDGKSALDYIAATPPDLIILDIMLPGMDGFEVCQMIKADPSTRHIPV
VMLTAKKSTQDRDKGLEAGASAYVTKPFKSARIIEIIQGLLAA

Sequences:

>Translated_123_residues
MEKKRILIVEDEESLLKLESILLSSRGYAVAGVTDGKSALDYIAATPPDLIILDIMLPGMDGFEVCQMIKADPSTRHIPV
VMLTAKKSTQDRDKGLEAGASAYVTKPFKSARIIEIIQGLLAA
>Mature_123_residues
MEKKRILIVEDEESLLKLESILLSSRGYAVAGVTDGKSALDYIAATPPDLIILDIMLPGMDGFEVCQMIKADPSTRHIPV
VMLTAKKSTQDRDKGLEAGASAYVTKPFKSARIIEIIQGLLAA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=118, Percent_Identity=39.8305084745763, Blast_Score=96, Evalue=6e-22,
Organism=Escherichia coli, GI1788394, Length=117, Percent_Identity=42.7350427350427, Blast_Score=91, Evalue=2e-20,
Organism=Escherichia coli, GI87082012, Length=117, Percent_Identity=36.7521367521368, Blast_Score=86, Evalue=8e-19,
Organism=Escherichia coli, GI1790860, Length=122, Percent_Identity=36.0655737704918, Blast_Score=78, Evalue=1e-16,
Organism=Escherichia coli, GI1789809, Length=120, Percent_Identity=32.5, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=34.1880341880342, Blast_Score=72, Evalue=7e-15,
Organism=Escherichia coli, GI1787375, Length=117, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=2e-14,
Organism=Escherichia coli, GI1788550, Length=118, Percent_Identity=30.5084745762712, Blast_Score=70, Evalue=6e-14,
Organism=Escherichia coli, GI1788191, Length=112, Percent_Identity=33.9285714285714, Blast_Score=68, Evalue=1e-13,
Organism=Escherichia coli, GI1789149, Length=108, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=8e-13,
Organism=Escherichia coli, GI1789402, Length=104, Percent_Identity=34.6153846153846, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1790552, Length=117, Percent_Identity=35.042735042735, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1788713, Length=107, Percent_Identity=35.5140186915888, Blast_Score=62, Evalue=9e-12,
Organism=Escherichia coli, GI1786911, Length=103, Percent_Identity=34.9514563106796, Blast_Score=61, Evalue=2e-11,
Organism=Escherichia coli, GI1790863, Length=122, Percent_Identity=30.327868852459, Blast_Score=60, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 13317; Mature: 13317

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKRILIVEDEESLLKLESILLSSRGYAVAGVTDGKSALDYIAATPPDLIILDIMLPGM
CCCCEEEEEECCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCEEEEEEECCCC
DGFEVCQMIKADPSTRHIPVVMLTAKKSTQDRDKGLEAGASAYVTKPFKSARIIEIIQGL
CHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHCCCCCCCCCEEECCCCCHHHHHHHHHH
LAA
HCC
>Mature Secondary Structure
MEKKRILIVEDEESLLKLESILLSSRGYAVAGVTDGKSALDYIAATPPDLIILDIMLPGM
CCCCEEEEEECCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCEEEEEEECCCC
DGFEVCQMIKADPSTRHIPVVMLTAKKSTQDRDKGLEAGASAYVTKPFKSARIIEIIQGL
CHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHCCCCCCCCCEEECCCCCHHHHHHHHHH
LAA
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA