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The map label for this gene is cheB-3

Identifier: 39997312

GI number: 39997312

Start: 2429043

End: 2430158

Strand: Reverse

Name: cheB-3

Synonym: GSU2214

Alternate gene names: 39997312

Gene position: 2430158-2429043 (Counterclockwise)

Preceding gene: 39997313

Following gene: 39997311

Centisome position: 63.71

GC content: 63.71

Gene sequence:

>1116_bases
ATGAGAAAGATCAGGGTCGTGGTCATCGACGATTCGGCCTACAGCCGCCGCGCCATCACCAAGATGCTCGAAAGCATGCC
CGAGGTGGAGGTGATCGGGTATGCCACCGACGGCGAGGAGGGTATCCGCAAAATTATCGACTTGAAACCGGACCTGGTGA
CCCTCGATCTTGAGATGCCCAGGATGGACGGGTTCACCCTCCTGCGGATCGTGATGGAATACTCTCCCACCGCCGTCATC
GTCATCAGTTCCAGGAGCGAGGACGAGAAGGTGTTCCGGGCGTTGGAACTGGGTGCAGTTGACTTTGTGGCAAAACCCAC
GAAGGGGGTGTCCGAGGAGATTCTCACCATCCGCGAGGACCTTCATCGCAAGGTGCGCGGCGTCATCCACCTGAACCTGG
CGGGCATCGTTCGTCGCGAGCGGGAACAGGAGCGAGCTTCTGTCGCGGCCGGCCGCAGGACCTCCGGCTCCGCGCCGTAT
GCAAAAGCAGCCGTGCGAACCGAGAGTACCGCTCCCCGCCCCGCCGGGCGCCTGGAGGTGGTGGCCATCGGTGCTTCCAC
GGGAGGGCCGCCGGCACTTCAGCGGATACTCTGTGCTCTGCCGGGGGCGTTTCCCCAGGCGGTCGTGGTCTCTCAACACA
TGCCGGCGGGATTCACGCGGACATTTGCGGAGCGTCTGAACCGGCTGTCACCCCTTGAGATATGCGAGGCAGCGGACGGT
GACGAGGTGCGGGCCGGGCGGGTGCTCATCGCCCCCGGCGGGCACAATATGGTCTTCGAGCGTCAGGGGAGCGAGGTGCG
TGCCCGGATCGTGAAGCCGGGCACGGATGACCGCTACGTGCCGTCCGTGGATGCCATGCTCCTTTCCTGCGCCGAGGTCT
TTGGTCCGCGTACGCTGGGCGTGGTGCTCACCGGTATGGGGAATGACGGCAGCAAAGGGGTCGCGGCCATCAATCGGGCA
GGGGGGCAGACCTTGGCCGAAGCTGAAGAGACGGCGGTGGTCTTCGGTATGCCCAAGGAGGCCATTGCCACCGGGGTGGT
TGACAAGATAGTGTCATTGGACCGCATGTCCCGCGAGATTATACAGCGGTGCGGTCTTCTCAGCGACGTCGATTGA

Upstream 100 bases:

>100_bases
GCCATTCCGAATCGCTGATGAACATATCCACGGCGTTTGCCCTCAAGCACCTGAAGAACGACATGGTCTACCAGAAACCT
CTCCCTCCGGGAGGCCGGCC

Downstream 100 bases:

>100_bases
CCGATCCCTCTTAGCCTGAGTGCTGAAGGGGCATAGGAGCGAGACATGTCAGTGGAAGAGGAAAAGGGGCTGCTGGCGCG
GGCCGATGAGTTTCTTCAGG

Product: protein-glutamate methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MRKIRVVVIDDSAYSRRAITKMLESMPEVEVIGYATDGEEGIRKIIDLKPDLVTLDLEMPRMDGFTLLRIVMEYSPTAVI
VISSRSEDEKVFRALELGAVDFVAKPTKGVSEEILTIREDLHRKVRGVIHLNLAGIVRREREQERASVAAGRRTSGSAPY
AKAAVRTESTAPRPAGRLEVVAIGASTGGPPALQRILCALPGAFPQAVVVSQHMPAGFTRTFAERLNRLSPLEICEAADG
DEVRAGRVLIAPGGHNMVFERQGSEVRARIVKPGTDDRYVPSVDAMLLSCAEVFGPRTLGVVLTGMGNDGSKGVAAINRA
GGQTLAEAEETAVVFGMPKEAIATGVVDKIVSLDRMSREIIQRCGLLSDVD

Sequences:

>Translated_371_residues
MRKIRVVVIDDSAYSRRAITKMLESMPEVEVIGYATDGEEGIRKIIDLKPDLVTLDLEMPRMDGFTLLRIVMEYSPTAVI
VISSRSEDEKVFRALELGAVDFVAKPTKGVSEEILTIREDLHRKVRGVIHLNLAGIVRREREQERASVAAGRRTSGSAPY
AKAAVRTESTAPRPAGRLEVVAIGASTGGPPALQRILCALPGAFPQAVVVSQHMPAGFTRTFAERLNRLSPLEICEAADG
DEVRAGRVLIAPGGHNMVFERQGSEVRARIVKPGTDDRYVPSVDAMLLSCAEVFGPRTLGVVLTGMGNDGSKGVAAINRA
GGQTLAEAEETAVVFGMPKEAIATGVVDKIVSLDRMSREIIQRCGLLSDVD
>Mature_371_residues
MRKIRVVVIDDSAYSRRAITKMLESMPEVEVIGYATDGEEGIRKIIDLKPDLVTLDLEMPRMDGFTLLRIVMEYSPTAVI
VISSRSEDEKVFRALELGAVDFVAKPTKGVSEEILTIREDLHRKVRGVIHLNLAGIVRREREQERASVAAGRRTSGSAPY
AKAAVRTESTAPRPAGRLEVVAIGASTGGPPALQRILCALPGAFPQAVVVSQHMPAGFTRTFAERLNRLSPLEICEAADG
DEVRAGRVLIAPGGHNMVFERQGSEVRARIVKPGTDDRYVPSVDAMLLSCAEVFGPRTLGVVLTGMGNDGSKGVAAINRA
GGQTLAEAEETAVVFGMPKEAIATGVVDKIVSLDRMSREIIQRCGLLSDVD

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=365, Percent_Identity=39.7260273972603, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI87082052, Length=104, Percent_Identity=29.8076923076923, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB3_GEOSL (P62640)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_953263.1
- ProteinModelPortal:   P62640
- SMR:   P62640
- GeneID:   2687095
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU2214
- NMPDR:   fig|243231.1.peg.2201
- TIGR:   GSU2214
- HOGENOM:   HBG705324
- OMA:   SQHMPAG
- ProtClustDB:   CLSK828757
- BioCyc:   GSUL243231:GSU_2214-MONOMER
- BRENDA:   3.1.1.61
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 39997; Mature: 39997

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 186-186 ACT_SITE 213-213 ACT_SITE 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIRVVVIDDSAYSRRAITKMLESMPEVEVIGYATDGEEGIRKIIDLKPDLVTLDLEMP
CCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCEEEEEECCC
RMDGFTLLRIVMEYSPTAVIVISSRSEDEKVFRALELGAVDFVAKPTKGVSEEILTIRED
CCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHH
LHRKVRGVIHLNLAGIVRREREQERASVAAGRRTSGSAPYAKAAVRTESTAPRPAGRLEV
HHHHHHHEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCEEE
VAIGASTGGPPALQRILCALPGAFPQAVVVSQHMPAGFTRTFAERLNRLSPLEICEAADG
EEEECCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHCCCC
DEVRAGRVLIAPGGHNMVFERQGSEVRARIVKPGTDDRYVPSVDAMLLSCAEVFGPRTLG
CCEECCEEEECCCCCCEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEE
VVLTGMGNDGSKGVAAINRAGGQTLAEAEETAVVFGMPKEAIATGVVDKIVSLDRMSREI
EEEEECCCCCCCCHHHHHHCCCCHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
IQRCGLLSDVD
HHHCCCCCCCC
>Mature Secondary Structure
MRKIRVVVIDDSAYSRRAITKMLESMPEVEVIGYATDGEEGIRKIIDLKPDLVTLDLEMP
CCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCEEEEEECCC
RMDGFTLLRIVMEYSPTAVIVISSRSEDEKVFRALELGAVDFVAKPTKGVSEEILTIRED
CCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHH
LHRKVRGVIHLNLAGIVRREREQERASVAAGRRTSGSAPYAKAAVRTESTAPRPAGRLEV
HHHHHHHEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCEEE
VAIGASTGGPPALQRILCALPGAFPQAVVVSQHMPAGFTRTFAERLNRLSPLEICEAADG
EEEECCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHCCCC
DEVRAGRVLIAPGGHNMVFERQGSEVRARIVKPGTDDRYVPSVDAMLLSCAEVFGPRTLG
CCEECCEEEECCCCCCEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEE
VVLTGMGNDGSKGVAAINRAGGQTLAEAEETAVVFGMPKEAIATGVVDKIVSLDRMSREI
EEEEECCCCCCCCHHHHHHCCCCHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
IQRCGLLSDVD
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA