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The map label for this gene is rpfG [H]

Identifier: 39997037

GI number: 39997037

Start: 2120881

End: 2123244

Strand: Direct

Name: rpfG [H]

Synonym: GSU1939

Alternate gene names: 39997037

Gene position: 2120881-2123244 (Clockwise)

Preceding gene: 39997036

Following gene: 39997044

Centisome position: 55.61

GC content: 56.13

Gene sequence:

>2364_bases
ATGACCCTCGCCGAAGAAAACCTGCGCCTCTTCGAGAAGAAGTTCGCGATCCTTCAGGAAATTTCGAACGCGCTCGTCGT
TACCGACAATATCAGCGCCATCGCCAACCTGATGCTCGATCTGGCAGTGAACTATACGAGGGCGGAAAAAGGCTCACTCC
TGCTTCTCAACGAGCGGGATGAGCTTTTCATCCTTGCCGGCCGCGGTATAGACCTTCAGCTCATGAGGACCTACCGGGCA
AGGCTCGGTGAAGGGATTGCCGGCATTGTCGCCAAGGGTCGCGTCCCCATCCTGGTAAAGGACATCGACCAGGATTCCCG
TTTTCAGGGAATGACCCGCGACCGGTACAAGACACCCTCGTTCATCTCATGTCCCATCGTCAGCAAGGATCGGCTGCTGG
GCCTGCTCAACATCAACGACAAGGCTGACGGCACGCCGTTTCTCGATGACGAATTCTCTCTCATAAAGATCATTGCCAAC
CAGGCGGCCATTGCCCTGGAAAACGCCATGCTGGTCAACCAGTTGCGGTACAAGGCGGGTGAGCAGGAGGGAATCAATCG
CAAGCTCATTGAAAGCGATGTTACCAAGACCGAATTCATCACCCGTGTGTCCCACGAGTTGCGCACCCCCCTCAATTCCA
TCAAGGGCTCCGTCTACTATCTCCAGCAATCGGACCGGCTCACCCGGTTGGAACAGCAGGAGTTCTACGACATCATTGCC
ACCGAGACCGACAAGCTCATCGCCATCGTTGAAAATCAGCTTGATTTCCTCCGCTTCGAAGATGAAACCATGATCGTCAC
CAAGACCGTGGTCAGTCTCAAGGAGGTTCTCAACGAAGTCCTCGGATCAAAAAACCTCAAAACGTTCCTGGCCCGCAAGA
ATATCACCGTCGATATCAAGATCGCCGAGGAGACATCGGACATCGTCGGCGACAAGGTGCGAATCGTCCAGTTTTTCCTG
AATATCATCGAAGGACTCTGCCACTTCCTTGAGCGGGGCGATGCAGTGACCGTAATGGTGGGCGAAAATGACAACGTGGA
GGTTTCGATCCAGCTCCCGCGCCAGATACCTGAAGCAATCCTCCCCTTCCTTTTCAGCTCCAGTCACATCTTCCAGGCCA
CTCAGCCCGATGAAAAGCTGAAGCTCTATCTTGCCCGCAAGGTGGTGGACCTCCACCAGTGGGAGTTGGTCGCGGAGAAC
GGGGAGCAGGGTCTCATCGTCAATCTGGCGATCCCCAAGAGTACCCGGCAGAAGGTCGACGCGGTCATGAACAACACCAT
CGAGATGTTCCTGGAGTTCATCGCGGAAGTGCTCGATCTCAACATCTGCTCCATCATGCTGTGCGACGATATCTCCGGCG
ACCTGGTCATCAAGAGTGCCCGAGGGCTTGAAGATTATCTCATCAAGCAGACGCGGGTGCGGCTGGGCGACCGGATCGCC
GGTTGGGTGGCTCTGGAAGGGAAACCGCTCTTCATCGAAGATATCGAGAAAGATCCGCGGTTCGGCAGAAGAAACATTTC
CCAGTACAACAACAAATCCCTCATCTCGCTCCCTCTCAGGGTCGGCGGCAAGGTCATCGGCGTGCTCAACCTCAACAACA
AGCGCACCGCAACCCCCTTCACCCGGCGTGACTACCAGGTGGCCTCCCTTCTGAGCGAACGGGTATCATACTTCATCGAG
CGACTTCATGCCGGCGAACACCATGAGGATAATTTCCGCAACTTCATGACGTCGTTCGACAACCTGATCACGGCCGAGAA
GAAGTATCACAAGAAGGATTCGATCCTGCCGGAACTTACGGTACGGATCATGGAACGGCTGGGTGCCGGAGAGGATATGT
GCAGGGTAGCGCTCTATGCGTCCATGCTCTACGACCTGGGACTCGTGGTGATGGAAAAGAGCATCCTCAACAAACGGGAA
ACGCTCCTGCCGGCCGAACGCAACAGCCTCAAGGTCCATCCCTACACCACGGTCGGGCTGATTAATGCCATCGAGTTTTC
CGAAGAGGTCAAGCGCGGCATCCTCCACCATCATGAGCACCTTGACGGCACCGGATACCCCGATGGTCTCCAGGGGGACG
ACATCCCGCTCATTTCGCGCGTCCTGGCAGTGGTCGACTCCTTCTGCGCCATGACGTCGGATCGCCCATACCGCAAGCGG
GTACCCGTCCGGCACGCGCTGCAAGAACTCAAAACAAAAGCGGGGACCGTGTACGATCCCCGCGTCGTGGATGCCCTTGA
AGCCGTTCTGGAGACTCAGAGGGAACAGGCCAAAGAAACCGGCCATGAACCTAAAGGTAAAACCGGTAACGGGTCTCGGG
CCGCAGGCGACAAAAAGAAAGAGCCCCTGTCAATGCACCCCTGA

Upstream 100 bases:

>100_bases
CCTAGTTGCTTTTTCTATTAATCAGGGAATATACAGCCATACGTAATGACGTGAACTCAATAGGTATGCTGCCGGTAACG
CAACCTGGAAACGGCCAACC

Downstream 100 bases:

>100_bases
CCTGACATCCCTCTTACCCACTTATCCCGTGTTTTTTCATCAGGTCGTAGATAGTTGGCCGGCTGATCCCCAACTCCTCG
GCTGCTCGGGCAATGTTCCC

Product: GAF domain/His kinase A domain/HD domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 787; Mature: 786

Protein sequence:

>787_residues
MTLAEENLRLFEKKFAILQEISNALVVTDNISAIANLMLDLAVNYTRAEKGSLLLLNERDELFILAGRGIDLQLMRTYRA
RLGEGIAGIVAKGRVPILVKDIDQDSRFQGMTRDRYKTPSFISCPIVSKDRLLGLLNINDKADGTPFLDDEFSLIKIIAN
QAAIALENAMLVNQLRYKAGEQEGINRKLIESDVTKTEFITRVSHELRTPLNSIKGSVYYLQQSDRLTRLEQQEFYDIIA
TETDKLIAIVENQLDFLRFEDETMIVTKTVVSLKEVLNEVLGSKNLKTFLARKNITVDIKIAEETSDIVGDKVRIVQFFL
NIIEGLCHFLERGDAVTVMVGENDNVEVSIQLPRQIPEAILPFLFSSSHIFQATQPDEKLKLYLARKVVDLHQWELVAEN
GEQGLIVNLAIPKSTRQKVDAVMNNTIEMFLEFIAEVLDLNICSIMLCDDISGDLVIKSARGLEDYLIKQTRVRLGDRIA
GWVALEGKPLFIEDIEKDPRFGRRNISQYNNKSLISLPLRVGGKVIGVLNLNNKRTATPFTRRDYQVASLLSERVSYFIE
RLHAGEHHEDNFRNFMTSFDNLITAEKKYHKKDSILPELTVRIMERLGAGEDMCRVALYASMLYDLGLVVMEKSILNKRE
TLLPAERNSLKVHPYTTVGLINAIEFSEEVKRGILHHHEHLDGTGYPDGLQGDDIPLISRVLAVVDSFCAMTSDRPYRKR
VPVRHALQELKTKAGTVYDPRVVDALEAVLETQREQAKETGHEPKGKTGNGSRAAGDKKKEPLSMHP

Sequences:

>Translated_787_residues
MTLAEENLRLFEKKFAILQEISNALVVTDNISAIANLMLDLAVNYTRAEKGSLLLLNERDELFILAGRGIDLQLMRTYRA
RLGEGIAGIVAKGRVPILVKDIDQDSRFQGMTRDRYKTPSFISCPIVSKDRLLGLLNINDKADGTPFLDDEFSLIKIIAN
QAAIALENAMLVNQLRYKAGEQEGINRKLIESDVTKTEFITRVSHELRTPLNSIKGSVYYLQQSDRLTRLEQQEFYDIIA
TETDKLIAIVENQLDFLRFEDETMIVTKTVVSLKEVLNEVLGSKNLKTFLARKNITVDIKIAEETSDIVGDKVRIVQFFL
NIIEGLCHFLERGDAVTVMVGENDNVEVSIQLPRQIPEAILPFLFSSSHIFQATQPDEKLKLYLARKVVDLHQWELVAEN
GEQGLIVNLAIPKSTRQKVDAVMNNTIEMFLEFIAEVLDLNICSIMLCDDISGDLVIKSARGLEDYLIKQTRVRLGDRIA
GWVALEGKPLFIEDIEKDPRFGRRNISQYNNKSLISLPLRVGGKVIGVLNLNNKRTATPFTRRDYQVASLLSERVSYFIE
RLHAGEHHEDNFRNFMTSFDNLITAEKKYHKKDSILPELTVRIMERLGAGEDMCRVALYASMLYDLGLVVMEKSILNKRE
TLLPAERNSLKVHPYTTVGLINAIEFSEEVKRGILHHHEHLDGTGYPDGLQGDDIPLISRVLAVVDSFCAMTSDRPYRKR
VPVRHALQELKTKAGTVYDPRVVDALEAVLETQREQAKETGHEPKGKTGNGSRAAGDKKKEPLSMHP
>Mature_786_residues
TLAEENLRLFEKKFAILQEISNALVVTDNISAIANLMLDLAVNYTRAEKGSLLLLNERDELFILAGRGIDLQLMRTYRAR
LGEGIAGIVAKGRVPILVKDIDQDSRFQGMTRDRYKTPSFISCPIVSKDRLLGLLNINDKADGTPFLDDEFSLIKIIANQ
AAIALENAMLVNQLRYKAGEQEGINRKLIESDVTKTEFITRVSHELRTPLNSIKGSVYYLQQSDRLTRLEQQEFYDIIAT
ETDKLIAIVENQLDFLRFEDETMIVTKTVVSLKEVLNEVLGSKNLKTFLARKNITVDIKIAEETSDIVGDKVRIVQFFLN
IIEGLCHFLERGDAVTVMVGENDNVEVSIQLPRQIPEAILPFLFSSSHIFQATQPDEKLKLYLARKVVDLHQWELVAENG
EQGLIVNLAIPKSTRQKVDAVMNNTIEMFLEFIAEVLDLNICSIMLCDDISGDLVIKSARGLEDYLIKQTRVRLGDRIAG
WVALEGKPLFIEDIEKDPRFGRRNISQYNNKSLISLPLRVGGKVIGVLNLNNKRTATPFTRRDYQVASLLSERVSYFIER
LHAGEHHEDNFRNFMTSFDNLITAEKKYHKKDSILPELTVRIMERLGAGEDMCRVALYASMLYDLGLVVMEKSILNKRET
LLPAERNSLKVHPYTTVGLINAIEFSEEVKRGILHHHEHLDGTGYPDGLQGDDIPLISRVLAVVDSFCAMTSDRPYRKRV
PVRHALQELKTKAGTVYDPRVVDALEAVLETQREQAKETGHEPKGKTGNGSRAAGDKKKEPLSMHP

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG2206

COG function: function code T; HD-GYP domain

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=172, Percent_Identity=27.906976744186, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: 2.7.3.- [C]

Molecular weight: Translated: 88927; Mature: 88796

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAEENLRLFEKKFAILQEISNALVVTDNISAIANLMLDLAVNYTRAEKGSLLLLNERD
CCCCHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
ELFILAGRGIDLQLMRTYRARLGEGIAGIVAKGRVPILVKDIDQDSRFQGMTRDRYKTPS
CEEEEECCCCCHHHHHHHHHHHCCCHHHHHHCCCCCEEEEECCCCCHHCCCCCCCCCCCC
FISCPIVSKDRLLGLLNINDKADGTPFLDDEFSLIKIIANQAAIALENAMLVNQLRYKAG
EEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EQEGINRKLIESDVTKTEFITRVSHELRTPLNSIKGSVYYLQQSDRLTRLEQQEFYDIIA
CCCCCCHHHHHHCCHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCHHHHHHHHHHHHHHH
TETDKLIAIVENQLDFLRFEDETMIVTKTVVSLKEVLNEVLGSKNLKTFLARKNITVDIK
CCCCCEEEEHHCCHHHEEECCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEE
IAEETSDIVGDKVRIVQFFLNIIEGLCHFLERGDAVTVMVGENDNVEVSIQLPRQIPEAI
ECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCHHHHHHH
LPFLFSSSHIFQATQPDEKLKLYLARKVVDLHQWELVAENGEQGLIVNLAIPKSTRQKVD
HHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEECCCHHHHHHH
AVMNNTIEMFLEFIAEVLDLNICSIMLCDDISGDLVIKSARGLEDYLIKQTRVRLGDRIA
HHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHCCHHE
GWVALEGKPLFIEDIEKDPRFGRRNISQYNNKSLISLPLRVGGKVIGVLNLNNKRTATPF
EEEEECCCEEEHCCCCCCCCCCHHHHHHCCCCCEEEEEHCCCCEEEEEEECCCCCCCCCC
TRRDYQVASLLSERVSYFIERLHAGEHHEDNFRNFMTSFDNLITAEKKYHKKDSILPELT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VRIMERLGAGEDMCRVALYASMLYDLGLVVMEKSILNKRETLLPAERNSLKVHPYTTVGL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHH
INAIEFSEEVKRGILHHHEHLDGTGYPDGLQGDDIPLISRVLAVVDSFCAMTSDRPYRKR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHC
VPVRHALQELKTKAGTVYDPRVVDALEAVLETQREQAKETGHEPKGKTGNGSRAAGDKKK
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
EPLSMHP
CCCCCCC
>Mature Secondary Structure 
TLAEENLRLFEKKFAILQEISNALVVTDNISAIANLMLDLAVNYTRAEKGSLLLLNERD
CCCHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
ELFILAGRGIDLQLMRTYRARLGEGIAGIVAKGRVPILVKDIDQDSRFQGMTRDRYKTPS
CEEEEECCCCCHHHHHHHHHHHCCCHHHHHHCCCCCEEEEECCCCCHHCCCCCCCCCCCC
FISCPIVSKDRLLGLLNINDKADGTPFLDDEFSLIKIIANQAAIALENAMLVNQLRYKAG
EEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EQEGINRKLIESDVTKTEFITRVSHELRTPLNSIKGSVYYLQQSDRLTRLEQQEFYDIIA
CCCCCCHHHHHHCCHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCHHHHHHHHHHHHHHH
TETDKLIAIVENQLDFLRFEDETMIVTKTVVSLKEVLNEVLGSKNLKTFLARKNITVDIK
CCCCCEEEEHHCCHHHEEECCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEE
IAEETSDIVGDKVRIVQFFLNIIEGLCHFLERGDAVTVMVGENDNVEVSIQLPRQIPEAI
ECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCHHHHHHH
LPFLFSSSHIFQATQPDEKLKLYLARKVVDLHQWELVAENGEQGLIVNLAIPKSTRQKVD
HHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEECCCHHHHHHH
AVMNNTIEMFLEFIAEVLDLNICSIMLCDDISGDLVIKSARGLEDYLIKQTRVRLGDRIA
HHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHCCHHE
GWVALEGKPLFIEDIEKDPRFGRRNISQYNNKSLISLPLRVGGKVIGVLNLNNKRTATPF
EEEEECCCEEEHCCCCCCCCCCHHHHHHCCCCCEEEEEHCCCCEEEEEEECCCCCCCCCC
TRRDYQVASLLSERVSYFIERLHAGEHHEDNFRNFMTSFDNLITAEKKYHKKDSILPELT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VRIMERLGAGEDMCRVALYASMLYDLGLVVMEKSILNKRETLLPAERNSLKVHPYTTVGL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHH
INAIEFSEEVKRGILHHHEHLDGTGYPDGLQGDDIPLISRVLAVVDSFCAMTSDRPYRKR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHC
VPVRHALQELKTKAGTVYDPRVVDALEAVLETQREQAKETGHEPKGKTGNGSRAAGDKKK
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
EPLSMHP
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]