Click here to switch to the map view.

The map label for this gene is nadB [H]

Identifier: 39996926

GI number: 39996926

Start: 1994578

End: 1996173

Strand: Direct

Name: nadB [H]

Synonym: GSU1827

Alternate gene names: 39996926

Gene position: 1994578-1996173 (Clockwise)

Preceding gene: 39996923

Following gene: 39996927

Centisome position: 52.29

GC content: 60.15

Gene sequence:

>1596_bases
ATGAAGATCCAGAGCGATTTTCTGGTCATTGGCAGCGGAATCGCCGGCCTCGCCTTCGCGCTCCAGGCAGCGCGTCATGG
AACCGTCGCAATAGTGACAAAACGCGAAGTAACCGAGTCGGCGACGAATTACGCCCAAGGTGGAATCGCATCGGTTTTTT
CCCAAGACGATACTTTTGACGCTCACGTGGAAGACACCCTGGTCGCCGGCGCGGGTATCTGCCACGAAGACGTGGTCAGA
ATGGTGGTGGAGGAAGGCCCGAAGGTCATCAGGAACCTGATCGAATGGGGAGTGCAGTTTACCCGCAGCGGCGAGGCCTT
CGACCTGACCCGCGAGGGCGGTCACAGCCAGCGGCGCATCCTGCACGCCGACGACGTCACCGGCCGCGAAATCGAGCGGG
CGCTGGTGGCGGCGGCCCGGGAAAACCCCAACATCCGCATTTACGAACACCATATTGCCATCGACCTCATCACAGAGGCG
AAAGTGACCCGCAAGCGGGTGGCCCCCAACCGCTGCCTCGGCGCTCATGTCCTCGGTATCGAAGACAATGTCGTGAGAAC
ATTCACCGCCAAGATCACCCTGCTTGCCACCGGCGGGGCGGGCAAGGTCTATCTCTACACCTGCAATCCCGACGTGGCCA
CGGGCGATGGCGTCGCCATGGCCTACCGTGCCGGCGCCACCATCGCCAATATGGAGTTCATGCAGTTCCACCCCACCACC
CTTTACCACCCCCATGCAAAATCCTTCCTCATATCCGAGGCGGTGCGTGGCGAAGGGGCGATTCTGCGGCGTCGCGACGG
CACCGCGTTCATGGAAAAATATCACAAGCTCAAGGACCTGGCTCCTCGCGACATCGTGGCGCGGGCCATCGACAACGAGA
TGAAAACCCATGGGGAAGACTGCGTCTTCCTTGACATTACCCACAAGGACCCCGAGTACGTCAGAAATCGCTTCCCCAAC
ATCTACCAGACCTGCCTTGAGTTTGGGCTCGACATGACCAGGGATCCGCTGCCGGTGGTTCCCGCAGCACACTATCTCTG
CGGTGGGGTGGCCGTGGATGCCAACGGCGAAACCGATATCCGCTACCTTTATGCGATCGGCGAAGCCGCCTTCACCGGCC
TGCACGGGGCAAACCGCCTGGCGAGCAACTCCCTGCTTGAGGCCGCGGTCTATGCCGGACGCGCCTATCAGCACGCAGTG
GAGGAACTGCGGCAGAACCACTTCGAATTCCCGGTCATTCCCGAGTGGGATTCAGGCACTGCCACGGATAGCGACGAGAT
GGTGGTCGTCTCCCAGAACTGGGACGAAATACGGCGCTTTATGTGGAATTACGTGGGAATCGTCCGTTCCGACAAGCGGC
TGGAGCGTGCCCTGCGGCGCATCCGCCTCATCCAGGAGGAAATCGAGGATTACTACTGGAACTTCACCGTAACTTCCGAC
CTGATCGAGCTGCGCAACATCGCCACCGTGGCCGAACTCATCGTCAAGTGCGCTCTCCAGCGCAAGGAATCCCGGGGACT
CCACTACACCATCAACTACCCGGAACGCGACGACATCCACTGCAGACACGATACCTTCATCAAGAAGCAATTCTGA

Upstream 100 bases:

>100_bases
ACCCTTGACAAACCACAGACGGGGGAGAATAATCGCCCAGTTATTCCGTTTACACACAAACCCTGCTGTTACTTTTCCTT
TCCCTTCTAGGAGACACGCA

Downstream 100 bases:

>100_bases
GGCGGCAATGACCATAGACCAGATACGCCAGGAAATCGACCGGCTGGACAGCGAACTGCTGCGGATCTTCAACCGTCGGG
CAGGGCTTGCCCTGGAAATC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MKIQSDFLVIGSGIAGLAFALQAARHGTVAIVTKREVTESATNYAQGGIASVFSQDDTFDAHVEDTLVAGAGICHEDVVR
MVVEEGPKVIRNLIEWGVQFTRSGEAFDLTREGGHSQRRILHADDVTGREIERALVAAARENPNIRIYEHHIAIDLITEA
KVTRKRVAPNRCLGAHVLGIEDNVVRTFTAKITLLATGGAGKVYLYTCNPDVATGDGVAMAYRAGATIANMEFMQFHPTT
LYHPHAKSFLISEAVRGEGAILRRRDGTAFMEKYHKLKDLAPRDIVARAIDNEMKTHGEDCVFLDITHKDPEYVRNRFPN
IYQTCLEFGLDMTRDPLPVVPAAHYLCGGVAVDANGETDIRYLYAIGEAAFTGLHGANRLASNSLLEAAVYAGRAYQHAV
EELRQNHFEFPVIPEWDSGTATDSDEMVVVSQNWDEIRRFMWNYVGIVRSDKRLERALRRIRLIQEEIEDYYWNFTVTSD
LIELRNIATVAELIVKCALQRKESRGLHYTINYPERDDIHCRHDTFIKKQF

Sequences:

>Translated_531_residues
MKIQSDFLVIGSGIAGLAFALQAARHGTVAIVTKREVTESATNYAQGGIASVFSQDDTFDAHVEDTLVAGAGICHEDVVR
MVVEEGPKVIRNLIEWGVQFTRSGEAFDLTREGGHSQRRILHADDVTGREIERALVAAARENPNIRIYEHHIAIDLITEA
KVTRKRVAPNRCLGAHVLGIEDNVVRTFTAKITLLATGGAGKVYLYTCNPDVATGDGVAMAYRAGATIANMEFMQFHPTT
LYHPHAKSFLISEAVRGEGAILRRRDGTAFMEKYHKLKDLAPRDIVARAIDNEMKTHGEDCVFLDITHKDPEYVRNRFPN
IYQTCLEFGLDMTRDPLPVVPAAHYLCGGVAVDANGETDIRYLYAIGEAAFTGLHGANRLASNSLLEAAVYAGRAYQHAV
EELRQNHFEFPVIPEWDSGTATDSDEMVVVSQNWDEIRRFMWNYVGIVRSDKRLERALRRIRLIQEEIEDYYWNFTVTSD
LIELRNIATVAELIVKCALQRKESRGLHYTINYPERDDIHCRHDTFIKKQF
>Mature_531_residues
MKIQSDFLVIGSGIAGLAFALQAARHGTVAIVTKREVTESATNYAQGGIASVFSQDDTFDAHVEDTLVAGAGICHEDVVR
MVVEEGPKVIRNLIEWGVQFTRSGEAFDLTREGGHSQRRILHADDVTGREIERALVAAARENPNIRIYEHHIAIDLITEA
KVTRKRVAPNRCLGAHVLGIEDNVVRTFTAKITLLATGGAGKVYLYTCNPDVATGDGVAMAYRAGATIANMEFMQFHPTT
LYHPHAKSFLISEAVRGEGAILRRRDGTAFMEKYHKLKDLAPRDIVARAIDNEMKTHGEDCVFLDITHKDPEYVRNRFPN
IYQTCLEFGLDMTRDPLPVVPAAHYLCGGVAVDANGETDIRYLYAIGEAAFTGLHGANRLASNSLLEAAVYAGRAYQHAV
EELRQNHFEFPVIPEWDSGTATDSDEMVVVSQNWDEIRRFMWNYVGIVRSDKRLERALRRIRLIQEEIEDYYWNFTVTSD
LIELRNIATVAELIVKCALQRKESRGLHYTINYPERDDIHCRHDTFIKKQF

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=535, Percent_Identity=31.214953271028, Blast_Score=221, Evalue=2e-57,
Organism=Escherichia coli, GI1788928, Length=515, Percent_Identity=54.3689320388349, Blast_Score=571, Evalue=1e-164,
Organism=Escherichia coli, GI1790597, Length=527, Percent_Identity=31.8785578747628, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1786942, Length=559, Percent_Identity=30.5903398926655, Blast_Score=204, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17550100, Length=539, Percent_Identity=30.6122448979592, Blast_Score=211, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI17505833, Length=535, Percent_Identity=30.2803738317757, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI71986328, Length=340, Percent_Identity=27.0588235294118, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322701, Length=539, Percent_Identity=32.8385899814471, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6322416, Length=546, Percent_Identity=31.8681318681319, Blast_Score=233, Evalue=5e-62,
Organism=Drosophila melanogaster, GI17137288, Length=544, Percent_Identity=30.8823529411765, Blast_Score=226, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24655642, Length=544, Percent_Identity=30.8823529411765, Blast_Score=226, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24655647, Length=544, Percent_Identity=30.8823529411765, Blast_Score=226, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24663005, Length=536, Percent_Identity=29.2910447761194, Blast_Score=196, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 59635; Mature: 59635

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIQSDFLVIGSGIAGLAFALQAARHGTVAIVTKREVTESATNYAQGGIASVFSQDDTFD
CCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCHHHHHCCCCCCC
AHVEDTLVAGAGICHEDVVRMVVEEGPKVIRNLIEWGVQFTRSGEAFDLTREGGHSQRRI
CHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHCCEECCCCCEEEECCCCCCCCCEE
LHADDVTGREIERALVAAARENPNIRIYEHHIAIDLITEAKVTRKRVAPNRCLGAHVLGI
EECCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEHHHHHHHHHCCCCCCCCEEEECC
EDNVVRTFTAKITLLATGGAGKVYLYTCNPDVATGDGVAMAYRAGATIANMEFMQFHPTT
CCCEEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCEEECEEEEEECCCE
LYHPHAKSFLISEAVRGEGAILRRRDGTAFMEKYHKLKDLAPRDIVARAIDNEMKTHGED
EECCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
CVFLDITHKDPEYVRNRFPNIYQTCLEFGLDMTRDPLPVVPAAHYLCGGVAVDANGETDI
EEEEEEECCCHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCEEECCCCCCCE
RYLYAIGEAAFTGLHGANRLASNSLLEAAVYAGRAYQHAVEELRQNHFEFPVIPEWDSGT
EEEEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
ATDSDEMVVVSQNWDEIRRFMWNYVGIVRSDKRLERALRRIRLIQEEIEDYYWNFTVTSD
CCCCCCEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEEHHH
LIELRNIATVAELIVKCALQRKESRGLHYTINYPERDDIHCRHDTFIKKQF
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MKIQSDFLVIGSGIAGLAFALQAARHGTVAIVTKREVTESATNYAQGGIASVFSQDDTFD
CCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCHHHHHCCCCCCC
AHVEDTLVAGAGICHEDVVRMVVEEGPKVIRNLIEWGVQFTRSGEAFDLTREGGHSQRRI
CHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHCCEECCCCCEEEECCCCCCCCCEE
LHADDVTGREIERALVAAARENPNIRIYEHHIAIDLITEAKVTRKRVAPNRCLGAHVLGI
EECCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEHHHHHHHHHCCCCCCCCEEEECC
EDNVVRTFTAKITLLATGGAGKVYLYTCNPDVATGDGVAMAYRAGATIANMEFMQFHPTT
CCCEEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCEEECEEEEEECCCE
LYHPHAKSFLISEAVRGEGAILRRRDGTAFMEKYHKLKDLAPRDIVARAIDNEMKTHGED
EECCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
CVFLDITHKDPEYVRNRFPNIYQTCLEFGLDMTRDPLPVVPAAHYLCGGVAVDANGETDI
EEEEEEECCCHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCEEECCCCCCCE
RYLYAIGEAAFTGLHGANRLASNSLLEAAVYAGRAYQHAVEELRQNHFEFPVIPEWDSGT
EEEEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
ATDSDEMVVVSQNWDEIRRFMWNYVGIVRSDKRLERALRRIRLIQEEIEDYYWNFTVTSD
CCCCCCEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEEHHH
LIELRNIATVAELIVKCALQRKESRGLHYTINYPERDDIHCRHDTFIKKQF
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7737518; 10984043; 7961421 [H]