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The map label for this gene is gluQ

Identifier: 39996431

GI number: 39996431

Start: 1453943

End: 1454887

Strand: Direct

Name: gluQ

Synonym: GSU1329

Alternate gene names: 39996431

Gene position: 1453943-1454887 (Clockwise)

Preceding gene: 39996430

Following gene: 39996432

Centisome position: 38.12

GC content: 71.96

Gene sequence:

>945_bases
ATGTGCGTCCCTTCCACCCCCCAGCCGCCGGTTATCGGCCGCTTCGCCCCGTCCCCCACGGGGCCGCTCCACGTGGGCTC
CCTCGTGGCGGCGGTGGCGAGCTACGCCATGGCCCGGCGCCAGGGGGGGCTCTGGCTCGTCCGCATGGAGGACCTGGATA
CCCCCCGGGTGGTGCCGGGGATGGCGGATGACATCCTGCGGACCCTGGAGTGCCTGGGGTTTGACTGGGATGGCGACATC
ATGCGGCAGAGCCGCCGCGCCGACGCCTACGGGGCCGCCCTCCAACGGCTCCTGGCGGCAGGGCACGCCTACCCCTGCGG
CTGCTCCCGGGCCGAGATCGCCCGGGCAGCCACCGCCCCCCACGACGGGGATGGCGAGATCCCCTATCCAAATCTTTGCC
GCCGGGGACTCCCGCCGGGCAAGGAACCCCGCTCCTTTCGCGTGCGGGTGCCGGCGGAGCCGGTGGAGTTCACGGATCTG
GTCATGGGACCGCAGCACCACGACCTCCCTGCGATGTGCGGCGACTTCGTGATCAAGCGGGCAGACGGTCTCTTCGCCTA
CCAGCTGGCCGTGGTGGTGGATGACGAGGCCCAGGGGGTGACCCAGGTGGTGCGCGGCGCCGACCTCCTTTCCTCTACCC
CCCGGCAGATCGTGCTCCAGCGGCTACTCGGCTTCGACACCCCCGTCTATGCCCACGTGCCGCTGGTGACAGGCCCCGGC
GGCGGCAAGCTCTCCAAGCGGGACAACGCCCTGTCCCTGGCCGCCGGCCGTGATCTGACGCGGGAAGGGGGCATGCTTCT
GCTGGCCGCCCTTCGCTTCCTGGGGCAGTCCCCGCCCGCGGAACTGGCCGGGGCATCCGGTGCCCGCGTACTCCGCTGGG
CCGCCGGCAATTTCGAGCCGTCGGCAATTCCGACCGCGGCCGCCCCCTTTCACGCTTCCCCCTGA

Upstream 100 bases:

>100_bases
CAATTCATCCTTTACCATGAAGGTGCTGTCCCTTACCGATTACTGCTGCTCGTTCCTCGGCTAGGCCGGCATCGTCTCGA
ACCGCTCCGGCAGACCGCCC

Downstream 100 bases:

>100_bases
AACCCGCCGTTTCCATCCCTTGATGTCATGCCGGAATCCATTGGCCCGTCATTGCGCAAGCTCGTTACGGGTCTATACTT
TTCGCAGAATATTCTCCGCT

Product: tRNA synthetase class I domain-containing protein

Products: NA

Alternate protein names: Glu-Q-RSs

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MCVPSTPQPPVIGRFAPSPTGPLHVGSLVAAVASYAMARRQGGLWLVRMEDLDTPRVVPGMADDILRTLECLGFDWDGDI
MRQSRRADAYGAALQRLLAAGHAYPCGCSRAEIARAATAPHDGDGEIPYPNLCRRGLPPGKEPRSFRVRVPAEPVEFTDL
VMGPQHHDLPAMCGDFVIKRADGLFAYQLAVVVDDEAQGVTQVVRGADLLSSTPRQIVLQRLLGFDTPVYAHVPLVTGPG
GGKLSKRDNALSLAAGRDLTREGGMLLLAALRFLGQSPPAELAGASGARVLRWAAGNFEPSAIPTAAAPFHASP

Sequences:

>Translated_314_residues
MCVPSTPQPPVIGRFAPSPTGPLHVGSLVAAVASYAMARRQGGLWLVRMEDLDTPRVVPGMADDILRTLECLGFDWDGDI
MRQSRRADAYGAALQRLLAAGHAYPCGCSRAEIARAATAPHDGDGEIPYPNLCRRGLPPGKEPRSFRVRVPAEPVEFTDL
VMGPQHHDLPAMCGDFVIKRADGLFAYQLAVVVDDEAQGVTQVVRGADLLSSTPRQIVLQRLLGFDTPVYAHVPLVTGPG
GGKLSKRDNALSLAAGRDLTREGGMLLLAALRFLGQSPPAELAGASGARVLRWAAGNFEPSAIPTAAAPFHASP
>Mature_314_residues
MCVPSTPQPPVIGRFAPSPTGPLHVGSLVAAVASYAMARRQGGLWLVRMEDLDTPRVVPGMADDILRTLECLGFDWDGDI
MRQSRRADAYGAALQRLLAAGHAYPCGCSRAEIARAATAPHDGDGEIPYPNLCRRGLPPGKEPRSFRVRVPAEPVEFTDL
VMGPQHHDLPAMCGDFVIKRADGLFAYQLAVVVDDEAQGVTQVVRGADLLSSTPRQIVLQRLLGFDTPVYAHVPLVTGPG
GGKLSKRDNALSLAAGRDLTREGGMLLLAALRFLGQSPPAELAGASGARVLRWAAGNFEPSAIPTAAAPFHASP

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily

Homologues:

Organism=Homo sapiens, GI134288884, Length=253, Percent_Identity=38.3399209486166, Blast_Score=154, Evalue=9e-38,
Organism=Escherichia coli, GI226510925, Length=296, Percent_Identity=46.6216216216216, Blast_Score=238, Evalue=3e-64,
Organism=Escherichia coli, GI1788743, Length=269, Percent_Identity=37.546468401487, Blast_Score=161, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI71993394, Length=253, Percent_Identity=33.596837944664, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324540, Length=303, Percent_Identity=34.983498349835, Blast_Score=165, Evalue=9e-42,
Organism=Drosophila melanogaster, GI24665321, Length=267, Percent_Identity=32.9588014981273, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLUQ_GEOSL (Q74DI7)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952382.1
- ProteinModelPortal:   Q74DI7
- SMR:   Q74DI7
- GeneID:   2688034
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1329
- NMPDR:   fig|243231.1.peg.1320
- TIGR:   GSU1329
- HOGENOM:   HBG628189
- OMA:   IIKRSDG
- ProtClustDB:   PRK05710
- BioCyc:   GSUL243231:GSU_1329-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01428
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR03838

Pfam domain/function: PF00749 tRNA-synt_1c

EC number: NA

Molecular weight: Translated: 33307; Mature: 33307

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 50-50 BINDING 187-187 BINDING 205-205 BINDING 246-246

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCVPSTPQPPVIGRFAPSPTGPLHVGSLVAAVASYAMARRQGGLWLVRMEDLDTPRVVPG
CCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCC
MADDILRTLECLGFDWDGDIMRQSRRADAYGAALQRLLAAGHAYPCGCSRAEIARAATAP
HHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCC
HDGDGEIPYPNLCRRGLPPGKEPRSFRVRVPAEPVEFTDLVMGPQHHDLPAMCGDFVIKR
CCCCCCCCCHHHHHCCCCCCCCCCCEEEECCCCCCCHHHHHCCCCCCCHHHHHHHHHHHH
ADGLFAYQLAVVVDDEAQGVTQVVRGADLLSSTPRQIVLQRLLGFDTPVYAHVPLVTGPG
CCCEEEEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEEECCC
GGKLSKRDNALSLAAGRDLTREGGMLLLAALRFLGQSPPAELAGASGARVLRWAAGNFEP
CCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCCCEEEEEECCCCCC
SAIPTAAAPFHASP
CCCCCCCCCCCCCC
>Mature Secondary Structure
MCVPSTPQPPVIGRFAPSPTGPLHVGSLVAAVASYAMARRQGGLWLVRMEDLDTPRVVPG
CCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCC
MADDILRTLECLGFDWDGDIMRQSRRADAYGAALQRLLAAGHAYPCGCSRAEIARAATAP
HHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCC
HDGDGEIPYPNLCRRGLPPGKEPRSFRVRVPAEPVEFTDLVMGPQHHDLPAMCGDFVIKR
CCCCCCCCCHHHHHCCCCCCCCCCCEEEECCCCCCCHHHHHCCCCCCCHHHHHHHHHHHH
ADGLFAYQLAVVVDDEAQGVTQVVRGADLLSSTPRQIVLQRLLGFDTPVYAHVPLVTGPG
CCCEEEEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEEECCC
GGKLSKRDNALSLAAGRDLTREGGMLLLAALRFLGQSPPAELAGASGARVLRWAAGNFEP
CCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCCCEEEEEECCCCCC
SAIPTAAAPFHASP
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA