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The map label for this gene is rsmE [H]

Identifier: 39995554

GI number: 39995554

Start: 477066

End: 477848

Strand: Reverse

Name: rsmE [H]

Synonym: GSU0446

Alternate gene names: 39995554

Gene position: 477848-477066 (Counterclockwise)

Preceding gene: 39995555

Following gene: 39995542

Centisome position: 12.53

GC content: 65.39

Gene sequence:

>783_bases
ATGCGCCGGTTTATCGTCACGGGCCTTGATCTGTCGGGTAAAAACGTGGTTGTGCGGGGGGACCTTTTTCGTCACATGGC
CCGGTCGCTTCGTCTAAAAATTGACACCGAGGTCATTCTCGCGGATGGGAAAGAGGCCGAGTGCACGGGAGTCATCACGG
CCATGGGACGAGACAGCCTGACGGTGACCATCAGCGAGCGGCGGCAGATCAGGGGGGATGGAGACGGCCTCTGGCTCACC
CTCTGCCAGGGTCTGCCCAAAGGCGACAAAATGGAACTCATCTTGCAGAAATCCACTGAACTCGGCGTATCCGAAGTGGT
TCCCTTCCTGGCCGAGCGCTCGGTCCCCCGCATTTCGCCGGAGCGGGAGCAGGAGCGGTTACGGCGCTGGCAACGGGTGG
CCGAAGAGGCAGCCCGCCAGGCTGGATGTCCAGCAGTACCCCGGGTACAACTGGCGCGGGGGCTGACCGAGGCGGTTCGG
GGAACGGACCACGACCTGAAGCTTCTGCTCTGGGAGGACGAACGGACCACGACCCTCGCCGGAGTCCTGGCCGCAGCGCA
GACACCGAAACGCATCGCCGTGGTGGTGGGCCCCGAGGGGGGATTCACGGCGGCCGAGGCAGCCGAGGCCCGGGAGGCGG
GATTCGTTCCCGTCACCCTCGGCCGTCGGATTCTCAGAACCGAAACCGCCGGCATCGCCCTGGTGGCGATACTGCAATAC
CTGTACGGAGACCTGGGCAGCGGGCCGCATCCCCGCCCCGCCCCGGCGCAACCGATACCATAG

Upstream 100 bases:

>100_bases
CCTTCGTCGTCGAGGGATTCGGCCGGAGCGGTCTCGCGCTGGTGGCAGTCTCCCGGGAAGGGGAATGGAGTTGTCTCGTA
TACCGGAACGAGGGGTGAGC

Downstream 100 bases:

>100_bases
CGGCCAGCATCGAAGCCGGCCGCACAAGGAGTTCCCACGATGAAATGTCCCAAGTGCGGCTACAACAGCTTCGAATTCCT
CGATAACTGCAAGAAGTGCA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MRRFIVTGLDLSGKNVVVRGDLFRHMARSLRLKIDTEVILADGKEAECTGVITAMGRDSLTVTISERRQIRGDGDGLWLT
LCQGLPKGDKMELILQKSTELGVSEVVPFLAERSVPRISPEREQERLRRWQRVAEEAARQAGCPAVPRVQLARGLTEAVR
GTDHDLKLLLWEDERTTTLAGVLAAAQTPKRIAVVVGPEGGFTAAEAAEAREAGFVPVTLGRRILRTETAGIALVAILQY
LYGDLGSGPHPRPAPAQPIP

Sequences:

>Translated_260_residues
MRRFIVTGLDLSGKNVVVRGDLFRHMARSLRLKIDTEVILADGKEAECTGVITAMGRDSLTVTISERRQIRGDGDGLWLT
LCQGLPKGDKMELILQKSTELGVSEVVPFLAERSVPRISPEREQERLRRWQRVAEEAARQAGCPAVPRVQLARGLTEAVR
GTDHDLKLLLWEDERTTTLAGVLAAAQTPKRIAVVVGPEGGFTAAEAAEAREAGFVPVTLGRRILRTETAGIALVAILQY
LYGDLGSGPHPRPAPAQPIP
>Mature_260_residues
MRRFIVTGLDLSGKNVVVRGDLFRHMARSLRLKIDTEVILADGKEAECTGVITAMGRDSLTVTISERRQIRGDGDGLWLT
LCQGLPKGDKMELILQKSTELGVSEVVPFLAERSVPRISPEREQERLRRWQRVAEEAARQAGCPAVPRVQLARGLTEAVR
GTDHDLKLLLWEDERTTTLAGVLAAAQTPKRIAVVVGPEGGFTAAEAAEAREAGFVPVTLGRRILRTETAGIALVAILQY
LYGDLGSGPHPRPAPAQPIP

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=235, Percent_Identity=33.1914893617021, Blast_Score=114, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 28295; Mature: 28295

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRFIVTGLDLSGKNVVVRGDLFRHMARSLRLKIDTEVILADGKEAECTGVITAMGRDSL
CCEEEEEECCCCCCEEEEECHHHHHHHHHHEEEECEEEEEECCCCCCCEEEEEECCCCCE
TVTISERRQIRGDGDGLWLTLCQGLPKGDKMELILQKSTELGVSEVVPFLAERSVPRISP
EEEEECCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCC
EREQERLRRWQRVAEEAARQAGCPAVPRVQLARGLTEAVRGTDHDLKLLLWEDERTTTLA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHH
GVLAAAQTPKRIAVVVGPEGGFTAAEAAEAREAGFVPVTLGRRILRTETAGIALVAILQY
HHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEHHHHHHHHHHHCCHHHHHHHHHH
LYGDLGSGPHPRPAPAQPIP
HHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRRFIVTGLDLSGKNVVVRGDLFRHMARSLRLKIDTEVILADGKEAECTGVITAMGRDSL
CCEEEEEECCCCCCEEEEECHHHHHHHHHHEEEECEEEEEECCCCCCCEEEEEECCCCCE
TVTISERRQIRGDGDGLWLTLCQGLPKGDKMELILQKSTELGVSEVVPFLAERSVPRISP
EEEEECCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCC
EREQERLRRWQRVAEEAARQAGCPAVPRVQLARGLTEAVRGTDHDLKLLLWEDERTTTLA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHH
GVLAAAQTPKRIAVVVGPEGGFTAAEAAEAREAGFVPVTLGRRILRTETAGIALVAILQY
HHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEHHHHHHHHHHHCCHHHHHHHHHH
LYGDLGSGPHPRPAPAQPIP
HHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]