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The map label for this gene is cheB-1

Identifier: 39995402

GI number: 39995402

Start: 321952

End: 323040

Strand: Reverse

Name: cheB-1

Synonym: GSU0293

Alternate gene names: 39995402

Gene position: 323040-321952 (Counterclockwise)

Preceding gene: 39995403

Following gene: 39995400

Centisome position: 8.47

GC content: 63.45

Gene sequence:

>1089_bases
ATGCTTCAGAATCAGACACGAAAACTGCGAGTCCTGGTCGTCGACGATTCTTCTTTCATGAGGATGGTGATCCGGAGCGT
ACTCGAAAAGGACCCGGCGATCGAGGTGGTCGGCATAGCCGTTGATGGGATGGAAGGGGTTGAAAAAGCCCTGGCCCTGA
AACCGGACCTGATCACCATGGACATCGAGATGCCACGGCTGGACGGGATCTCCGCCCTCAAACAGGTCATGGCCAAGTGC
CCGACACGGGTCCTGATGGTCTCAACCCTCACCTGCGAGGGAGCCAAGGCCACCTTTGACGCCCTCGATGCAGGCGCCAT
CGACTACATTCCGAAAAACGTGACCGACAGCGCCGATGCCCAGCGAGTCTTCCGGGAAGAGTTGCTGCGCAAGGTCAAGG
GAGCCGCCAGCTCTATCTTCGGCCGGCCGATGACCACATCGGCCCCCCGGGCGATCATCGCTCCCCCCCGGCAGGTGCAG
CCCGCTCCGCGCCCCTCGCAGGCATTGGCCGGCAAGTTCCACTACGTGGGCATTGGCGCCTCCACCGGCGGCCCGGTGGC
GCTCCAGGAGGTCCTGGGCCGCATCCCCGGCAACTACCCCCATGGCATCGTGGTGGCCATCCACATGCCCAAGGCATTCA
CGGGGCCCTACGCCGAACGACTCAACAGCAAGTGTTCCCTCCAGATCAAGGAGGCGAATGATGGCGACATCATCCAGCCC
GGCGTGGTCCTCGTTGCCCCGGGCGGTCGTCACATGGCGCTGGCCCGCCAGGGAAACTCGATCGTGGTTCGGACGCTCTC
CACCGCCGAGTGCCCCCAGTACATCTACATTCCGTCGGTGGACCACATGATGACCACCCTGGCTGACGCCACCAACGGAT
CGGCCCTGGGAGTGATCCTTACGGGCATGGGGAGCGACGGATTCAAGGGGATGAAGCACTTGAAAAGCAAGGGTGGCATC
ACCATCGTCCAGGACGAGGCCACCTCCACCATCTACGGCATGCCGCGGGCCTGCATCGAGGGGGGCGTCGCAGACACGGT
GCTTCCCCTCACCCAGATCGGTTCGGAGATCGCCCGTCTGGGTGGGTGA

Upstream 100 bases:

>100_bases
CTCCGAGCTCGACATTGAGCGGTTCTGCTACGAACTGGATGACCGGGTGGAAGAAATCAGTGAGTTGGTAGGTAATATCT
TCAAGAACGGGTGATTGTGC

Downstream 100 bases:

>100_bases
TCAACGGCGCGCCGAGAGCAGGATACCTCCCTCACGGATCAGGGCACGGCACAGGGAGAAGCCGGTCCGCTTGAGACGGA
ACAACAACAACGGTCCGGCC

Product: protein-glutamate methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MLQNQTRKLRVLVVDDSSFMRMVIRSVLEKDPAIEVVGIAVDGMEGVEKALALKPDLITMDIEMPRLDGISALKQVMAKC
PTRVLMVSTLTCEGAKATFDALDAGAIDYIPKNVTDSADAQRVFREELLRKVKGAASSIFGRPMTTSAPRAIIAPPRQVQ
PAPRPSQALAGKFHYVGIGASTGGPVALQEVLGRIPGNYPHGIVVAIHMPKAFTGPYAERLNSKCSLQIKEANDGDIIQP
GVVLVAPGGRHMALARQGNSIVVRTLSTAECPQYIYIPSVDHMMTTLADATNGSALGVILTGMGSDGFKGMKHLKSKGGI
TIVQDEATSTIYGMPRACIEGGVADTVLPLTQIGSEIARLGG

Sequences:

>Translated_362_residues
MLQNQTRKLRVLVVDDSSFMRMVIRSVLEKDPAIEVVGIAVDGMEGVEKALALKPDLITMDIEMPRLDGISALKQVMAKC
PTRVLMVSTLTCEGAKATFDALDAGAIDYIPKNVTDSADAQRVFREELLRKVKGAASSIFGRPMTTSAPRAIIAPPRQVQ
PAPRPSQALAGKFHYVGIGASTGGPVALQEVLGRIPGNYPHGIVVAIHMPKAFTGPYAERLNSKCSLQIKEANDGDIIQP
GVVLVAPGGRHMALARQGNSIVVRTLSTAECPQYIYIPSVDHMMTTLADATNGSALGVILTGMGSDGFKGMKHLKSKGGI
TIVQDEATSTIYGMPRACIEGGVADTVLPLTQIGSEIARLGG
>Mature_362_residues
MLQNQTRKLRVLVVDDSSFMRMVIRSVLEKDPAIEVVGIAVDGMEGVEKALALKPDLITMDIEMPRLDGISALKQVMAKC
PTRVLMVSTLTCEGAKATFDALDAGAIDYIPKNVTDSADAQRVFREELLRKVKGAASSIFGRPMTTSAPRAIIAPPRQVQ
PAPRPSQALAGKFHYVGIGASTGGPVALQEVLGRIPGNYPHGIVVAIHMPKAFTGPYAERLNSKCSLQIKEANDGDIIQP
GVVLVAPGGRHMALARQGNSIVVRTLSTAECPQYIYIPSVDHMMTTLADATNGSALGVILTGMGSDGFKGMKHLKSKGGI
TIVQDEATSTIYGMPRACIEGGVADTVLPLTQIGSEIARLGG

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=350, Percent_Identity=35.1428571428571, Blast_Score=214, Evalue=5e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB1_GEOSL (P62638)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951353.1
- ProteinModelPortal:   P62638
- SMR:   P62638
- GeneID:   2686644
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0293
- NMPDR:   fig|243231.1.peg.291
- TIGR:   GSU0293
- HOGENOM:   HBG705324
- OMA:   NANIIME
- ProtClustDB:   CLSK827727
- BioCyc:   GSUL243231:GSU_0293-MONOMER
- BRENDA:   3.1.1.61
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 38340; Mature: 38340

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 181-181 ACT_SITE 208-208 ACT_SITE 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQNQTRKLRVLVVDDSSFMRMVIRSVLEKDPAIEVVGIAVDGMEGVEKALALKPDLITM
CCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHCCCCEEEE
DIEMPRLDGISALKQVMAKCPTRVLMVSTLTCEGAKATFDALDAGAIDYIPKNVTDSADA
EECCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHCCCCHHCCCCCCCCCHHH
QRVFREELLRKVKGAASSIFGRPMTTSAPRAIIAPPRQVQPAPRPSQALAGKFHYVGIGA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCHHHCCCEEEEEEEC
STGGPVALQEVLGRIPGNYPHGIVVAIHMPKAFTGPYAERLNSKCSLQIKEANDGDIIQP
CCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHCCCCEEEEEEECCCCCEECC
GVVLVAPGGRHMALARQGNSIVVRTLSTAECPQYIYIPSVDHMMTTLADATNGSALGVIL
CEEEECCCCCEEEEEECCCEEEEEEECCCCCCCEEECCCHHHHHHHHHHCCCCCEEEEEE
TGMGSDGFKGMKHLKSKGGITIVQDEATSTIYGMPRACIEGGVADTVLPLTQIGSEIARL
ECCCCCCHHHHHHHHHCCCEEEEECCCCCHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHH
GG
CC
>Mature Secondary Structure
MLQNQTRKLRVLVVDDSSFMRMVIRSVLEKDPAIEVVGIAVDGMEGVEKALALKPDLITM
CCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHCCCCEEEE
DIEMPRLDGISALKQVMAKCPTRVLMVSTLTCEGAKATFDALDAGAIDYIPKNVTDSADA
EECCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHCCCCHHCCCCCCCCCHHH
QRVFREELLRKVKGAASSIFGRPMTTSAPRAIIAPPRQVQPAPRPSQALAGKFHYVGIGA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCHHHCCCEEEEEEEC
STGGPVALQEVLGRIPGNYPHGIVVAIHMPKAFTGPYAERLNSKCSLQIKEANDGDIIQP
CCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHCCCCEEEEEEECCCCCEECC
GVVLVAPGGRHMALARQGNSIVVRTLSTAECPQYIYIPSVDHMMTTLADATNGSALGVIL
CEEEECCCCCEEEEEECCCEEEEEEECCCCCCCEEECCCHHHHHHHHHHCCCCCEEEEEE
TGMGSDGFKGMKHLKSKGGITIVQDEATSTIYGMPRACIEGGVADTVLPLTQIGSEIARL
ECCCCCCHHHHHHHHHCCCEEEEECCCCCHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHH
GG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA