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The map label for this gene is pilT-2 [H]

Identifier: 39995340

GI number: 39995340

Start: 237886

End: 238956

Strand: Reverse

Name: pilT-2 [H]

Synonym: GSU0230

Alternate gene names: 39995340

Gene position: 238956-237886 (Counterclockwise)

Preceding gene: 39995341

Following gene: 39995338

Centisome position: 6.27

GC content: 62.37

Gene sequence:

>1071_bases
ATGGATATGAACCTTCTGTCCCAGATTCTGGGCATTGCCTTTGAAAAGCGGGTTTCCGACCTCCACTTCGAGGTGGACAA
TCCCCCCTTCTTCCGTGCCAAGGGGCAGTTGCTCCGCTCAAAGTTGCCGAAGCTCTCCCCCCAGGACACCGAATTCATCG
CCCGGGCCGTTATGGAGCAGAACCACCGGACCCTCCCCGACGAGCTGCGGGAGCTGGACGCCTCCTACTCGCTCCCCAAT
GGCGGGCGCTTCCGGGTGAGCATCTTCCGTCAGCGCGGGAGCATCGGCATCGTCATGAGGGTGATTCCTCCCCACGTGGG
GACCTTCGAGGAACTCAACCTGCCGCCGGTCCTGGGAGAGATCGCCAAAGCCCCCAACGGCCTCGTGCTGGTCACCGGCC
CCACGGGCAACGGTAAGTCGACCACCCTTGCCTCCATGATTCGCCACCTGAACGAGACCTGCACCTTCAACATCATCACC
ATCGAGGACCCCATCGAGTTCCTCTTCACCTCGGACAAGAGCTGCATCATCCAGCGGGAGGTGGGAATCGACACGGTCGA
CTTCAGCGCGGCGCTCCGCTCGTCCCTGCGGATGGACCCCGACGTGATCATGGTGGGCGAGATGCGCGACCTGGAAACCA
TCGACGCCTGCATCAAGGCGGCGGAGACGGGCCACCTGGTCTTTTCCACCCTCCACACCCAGAGCGCCGTATCCACTATC
AACCGGCTCATCGGCCACTTCCCCCCCGATGCCCAGGAGGTCCTGCGCCAGCGCCTGGCCGACATCCTGGTGGCCACCGT
GTCGTTACGGCTCATCAAGGACAAATCCGGGGAGAACATCCTGCCGGTGGTGGAGGTGATGCGAGCCACAACCACCATCC
AGGCCTGCATTCGCGAGGGACGCCTGGACGAGATCGAGAAGCACATCGAAAACGGCCGCTCCCTCTACCAGATGCAGACC
CTTGACCAGCACCTGCTGGAACTGTGCGAGAAGGACGTCATTACCTTCGACCAGGCCAAGCAGATCACCCGCTCCATGGA
CCTTGAGCGGAAGCTGGCCTTCACCGAGTAG

Upstream 100 bases:

>100_bases
CTATCGCAGGAAACGGGAGCGGAAGGCTCCCGAGGGGACGGCCTGTGACCGCTGAACGGTCTTTCCGGCCGCATTTTTTA
GATAGCAGTGAGGTACCGCC

Downstream 100 bases:

>100_bases
CCGTCCTATGCCAGGTACTTCTCCCGCAGGGTCACTTTGCTGATCTTGCCCACGCTCGTCTTGTCGATGGCATCCACGAG
CCGCACTTTCAGCAGGACCA

Product: twitching motility protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MDMNLLSQILGIAFEKRVSDLHFEVDNPPFFRAKGQLLRSKLPKLSPQDTEFIARAVMEQNHRTLPDELRELDASYSLPN
GGRFRVSIFRQRGSIGIVMRVIPPHVGTFEELNLPPVLGEIAKAPNGLVLVTGPTGNGKSTTLASMIRHLNETCTFNIIT
IEDPIEFLFTSDKSCIIQREVGIDTVDFSAALRSSLRMDPDVIMVGEMRDLETIDACIKAAETGHLVFSTLHTQSAVSTI
NRLIGHFPPDAQEVLRQRLADILVATVSLRLIKDKSGENILPVVEVMRATTTIQACIREGRLDEIEKHIENGRSLYQMQT
LDQHLLELCEKDVITFDQAKQITRSMDLERKLAFTE

Sequences:

>Translated_356_residues
MDMNLLSQILGIAFEKRVSDLHFEVDNPPFFRAKGQLLRSKLPKLSPQDTEFIARAVMEQNHRTLPDELRELDASYSLPN
GGRFRVSIFRQRGSIGIVMRVIPPHVGTFEELNLPPVLGEIAKAPNGLVLVTGPTGNGKSTTLASMIRHLNETCTFNIIT
IEDPIEFLFTSDKSCIIQREVGIDTVDFSAALRSSLRMDPDVIMVGEMRDLETIDACIKAAETGHLVFSTLHTQSAVSTI
NRLIGHFPPDAQEVLRQRLADILVATVSLRLIKDKSGENILPVVEVMRATTTIQACIREGRLDEIEKHIENGRSLYQMQT
LDQHLLELCEKDVITFDQAKQITRSMDLERKLAFTE
>Mature_356_residues
MDMNLLSQILGIAFEKRVSDLHFEVDNPPFFRAKGQLLRSKLPKLSPQDTEFIARAVMEQNHRTLPDELRELDASYSLPN
GGRFRVSIFRQRGSIGIVMRVIPPHVGTFEELNLPPVLGEIAKAPNGLVLVTGPTGNGKSTTLASMIRHLNETCTFNIIT
IEDPIEFLFTSDKSCIIQREVGIDTVDFSAALRSSLRMDPDVIMVGEMRDLETIDACIKAAETGHLVFSTLHTQSAVSTI
NRLIGHFPPDAQEVLRQRLADILVATVSLRLIKDKSGENILPVVEVMRATTTIQACIREGRLDEIEKHIENGRSLYQMQT
LDQHLLELCEKDVITFDQAKQITRSMDLERKLAFTE

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=313, Percent_Identity=39.297124600639, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI1786296, Length=286, Percent_Identity=37.4125874125874, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1789723, Length=264, Percent_Identity=32.9545454545455, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39930; Mature: 39930

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMNLLSQILGIAFEKRVSDLHFEVDNPPFFRAKGQLLRSKLPKLSPQDTEFIARAVMEQ
CCHHHHHHHHHHHHHHHHHHCEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
NHRTLPDELRELDASYSLPNGGRFRVSIFRQRGSIGIVMRVIPPHVGTFEELNLPPVLGE
CCCCCCHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCCHHHCCCCHHHHH
IAKAPNGLVLVTGPTGNGKSTTLASMIRHLNETCTFNIITIEDPIEFLFTSDKSCIIQRE
HHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCHHHHCCCCCCEEEEEC
VGIDTVDFSAALRSSLRMDPDVIMVGEMRDLETIDACIKAAETGHLVFSTLHTQSAVSTI
CCCCEEHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NRLIGHFPPDAQEVLRQRLADILVATVSLRLIKDKSGENILPVVEVMRATTTIQACIREG
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHCC
RLDEIEKHIENGRSLYQMQTLDQHLLELCEKDVITFDQAKQITRSMDLERKLAFTE
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHCCCC
>Mature Secondary Structure
MDMNLLSQILGIAFEKRVSDLHFEVDNPPFFRAKGQLLRSKLPKLSPQDTEFIARAVMEQ
CCHHHHHHHHHHHHHHHHHHCEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
NHRTLPDELRELDASYSLPNGGRFRVSIFRQRGSIGIVMRVIPPHVGTFEELNLPPVLGE
CCCCCCHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCCHHHCCCCHHHHH
IAKAPNGLVLVTGPTGNGKSTTLASMIRHLNETCTFNIITIEDPIEFLFTSDKSCIIQRE
HHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCHHHHCCCCCCEEEEEC
VGIDTVDFSAALRSSLRMDPDVIMVGEMRDLETIDACIKAAETGHLVFSTLHTQSAVSTI
CCCCEEHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NRLIGHFPPDAQEVLRQRLADILVATVSLRLIKDKSGENILPVVEVMRATTTIQACIREG
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHCC
RLDEIEKHIENGRSLYQMQTLDQHLLELCEKDVITFDQAKQITRSMDLERKLAFTE
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]