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The map label for this gene is ttcA

Identifier: 39995238

GI number: 39995238

Start: 141097

End: 141945

Strand: Reverse

Name: ttcA

Synonym: GSU0127

Alternate gene names: 39995238

Gene position: 141945-141097 (Counterclockwise)

Preceding gene: 39995243

Following gene: 39995237

Centisome position: 3.72

GC content: 62.07

Gene sequence:

>849_bases
ATGCCCGGATGGCACGCCTTGACGAAACCGGCGCGTTTCCGCTACCCTGAGGGCTTGCACGGAAGGGAGATTCGGGTGGA
ATTCATCGATAACATACGATACAAGAAGATAAAAAATGCCGTGGGCCGGGCAGTGGCCGACTTCGGCCTCATCCGGGACG
GGGATCGGATCGCGGTGGCGGTGTCCGGGGGCAAAGACTCGTACACCCTGCTGCACATCCTTGAGGGACTGCGGCGCCGG
GCACCGGTGAAATACGAGCTGGTTGCCGTCACCATCGATTCCGGCTACCCCGGATTCCGCAGCGACGTGATAGCCTCCTA
CTTGCGGGAACAAGGGTTTGCCCACCACCTGGAGACCACGGACCACTATGACATCATCCGCGAAAAGCGCCGCCCCGGCT
CGTCCTACTGCTCCATCTGCGCCCGGCTGAAGCGCGGCGTCCTCTATACCCTGGCCCAGAAATTGGGCTGCAACAAACTG
GCCCTGGGACATCACCTGGACGATTTCGTGGAGACCCTGCTTCTGAACCAGTTCTTTGTGGGGACCCTCAAAGCCATGGC
GCCCCGGATGCTGGCCGACAACGGCGAGACAACGGTAATCCGGCCGCTGGTCTACGTGGAGGAACGGGAGATCATCCCCT
TTGCCCGGGAGAACCGCTTTCCGGTGGTTTGCTGCTCTTGCCCGGTCTGCGGCACCGCTGATCTCCAGCGCCGGCGGATG
AAAAAGCTCCTGACCGAACTGGAAAAGGAAAACCCGGCAGTCAAGCGGAGCCTGCTGCGCGCTCTGGGCAACGTGCAGCC
GCGCTACCTCCTCGACCTCGAACTGCAGCGGCTCTGCGACGTTCCCTGA

Upstream 100 bases:

>100_bases
ACCGCCACGTGGACAATGACGGTCTGCCGTTCGTTCACGGGGAATGATGACATGGCCCCATGGTACCAGCAGTCGGGCAT
CCGGACAAGGAGAGACCTGA

Downstream 100 bases:

>100_bases
CCAGGGGACGGGGTTGTGAGGCCCCCTGTGCCTCCCCTCGGGGAACGGGCCGGTCGCGCAACACGTCCGCAAGGGCGATG
CCGGCACAGTAGACAACGAG

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MPGWHALTKPARFRYPEGLHGREIRVEFIDNIRYKKIKNAVGRAVADFGLIRDGDRIAVAVSGGKDSYTLLHILEGLRRR
APVKYELVAVTIDSGYPGFRSDVIASYLREQGFAHHLETTDHYDIIREKRRPGSSYCSICARLKRGVLYTLAQKLGCNKL
ALGHHLDDFVETLLLNQFFVGTLKAMAPRMLADNGETTVIRPLVYVEEREIIPFARENRFPVVCCSCPVCGTADLQRRRM
KKLLTELEKENPAVKRSLLRALGNVQPRYLLDLELQRLCDVP

Sequences:

>Translated_282_residues
MPGWHALTKPARFRYPEGLHGREIRVEFIDNIRYKKIKNAVGRAVADFGLIRDGDRIAVAVSGGKDSYTLLHILEGLRRR
APVKYELVAVTIDSGYPGFRSDVIASYLREQGFAHHLETTDHYDIIREKRRPGSSYCSICARLKRGVLYTLAQKLGCNKL
ALGHHLDDFVETLLLNQFFVGTLKAMAPRMLADNGETTVIRPLVYVEEREIIPFARENRFPVVCCSCPVCGTADLQRRRM
KKLLTELEKENPAVKRSLLRALGNVQPRYLLDLELQRLCDVP
>Mature_281_residues
PGWHALTKPARFRYPEGLHGREIRVEFIDNIRYKKIKNAVGRAVADFGLIRDGDRIAVAVSGGKDSYTLLHILEGLRRRA
PVKYELVAVTIDSGYPGFRSDVIASYLREQGFAHHLETTDHYDIIREKRRPGSSYCSICARLKRGVLYTLAQKLGCNKLA
LGHHLDDFVETLLLNQFFVGTLKAMAPRMLADNGETTVIRPLVYVEEREIIPFARENRFPVVCCSCPVCGTADLQRRRMK
KLLTELEKENPAVKRSLLRALGNVQPRYLLDLELQRLCDVP

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Homo sapiens, GI222537719, Length=224, Percent_Identity=28.125, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1787606, Length=242, Percent_Identity=42.5619834710744, Blast_Score=216, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI193211090, Length=245, Percent_Identity=37.1428571428571, Blast_Score=144, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17540090, Length=257, Percent_Identity=27.2373540856031, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24651972, Length=223, Percent_Identity=29.5964125560538, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_GEOSL (Q74GW7)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951189.1
- ProteinModelPortal:   Q74GW7
- GeneID:   2688089
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0127
- NMPDR:   fig|243231.1.peg.127
- TIGR:   GSU0127
- HOGENOM:   HBG347038
- OMA:   PHEILTR
- ProtClustDB:   PRK10696
- BioCyc:   GSUL243231:GSU_0127-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 32076; Mature: 31945

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGWHALTKPARFRYPEGLHGREIRVEFIDNIRYKKIKNAVGRAVADFGLIRDGDRIAVA
CCCCCCCCCCHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCEECCCEEEEE
VSGGKDSYTLLHILEGLRRRAPVKYELVAVTIDSGYPGFRSDVIASYLREQGFAHHLETT
EECCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHCCCC
DHYDIIREKRRPGSSYCSICARLKRGVLYTLAQKLGCNKLALGHHLDDFVETLLLNQFFV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GTLKAMAPRMLADNGETTVIRPLVYVEEREIIPFARENRFPVVCCSCPVCGTADLQRRRM
HHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHCCCCCCEEEECCCCCCCHHHHHHHH
KKLLTELEKENPAVKRSLLRALGNVQPRYLLDLELQRLCDVP
HHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEHHHHHHHCCCC
>Mature Secondary Structure 
PGWHALTKPARFRYPEGLHGREIRVEFIDNIRYKKIKNAVGRAVADFGLIRDGDRIAVA
CCCCCCCCCHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCEECCCEEEEE
VSGGKDSYTLLHILEGLRRRAPVKYELVAVTIDSGYPGFRSDVIASYLREQGFAHHLETT
EECCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHCCCC
DHYDIIREKRRPGSSYCSICARLKRGVLYTLAQKLGCNKLALGHHLDDFVETLLLNQFFV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GTLKAMAPRMLADNGETTVIRPLVYVEEREIIPFARENRFPVVCCSCPVCGTADLQRRRM
HHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHCCCCCCEEEECCCCCCCHHHHHHHH
KKLLTELEKENPAVKRSLLRALGNVQPRYLLDLELQRLCDVP
HHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA