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The map label for this gene is narH [H]

Identifier: 38637932

GI number: 38637932

Start: 291102

End: 292652

Strand: Direct

Name: narH [H]

Synonym: PHG271

Alternate gene names: 38637932

Gene position: 291102-292652 (Clockwise)

Preceding gene: 38637931

Following gene: 38637933

Centisome position: 64.38

GC content: 65.51

Gene sequence:

>1551_bases
ATGAAGATCCGTGCCCAGGTGGCCATGGTGCTGAACCTCGACAAGTGCATCGGCTGCCATACCTGCTCCGTGACCTGCAA
GAACGTCTGGACCAGCCGCGACGGCGTCGAGTACGCCTGGTTCAACAACGTCGAGACCAAGCCCGGCATCGGCTACCCCA
AGGAATGGGAGAACCAGGACAAGTGGCAGGGTGGCTGGAAGCGCAACGCCGACGGCACGCTGGAGCCGCGCCAGGGCGGC
AAGCTGAAGATCCTCGCGAACCTGTTCGCCAACCCCAACCTGCCGGCGATCGACGAGTACTACGAGCCCTTCACCTACGA
CTACGAGCACCTGCAGAAGGCGCCGCTGGTCCAGACCCCGCCCACCGCGCGCCCGGTGTCCGTGCTGACCGGCCGCAAGA
TGGAAAAGATCGAATGGGGCCCAAACTGGGAAGACGACCTCGGCGGCGAGTTCAGCTCGCGCGGCCGCGACAAGCTCTTC
GACAACGTGCAGAAGGAGATGTACTCGACCTTCGAGAACACCTTCATGATGTATCTGCCGCGCCTGTGCGAGCACTGCCT
GAACCCGGCCTGCGTGGCCTCGTGCCCGTCGGGCTCGATCTACAAGCGCGAGGACGACGGCATCGTGCTGGTCGACCAGG
ACAAGTGCCGCGGCTGGCGCATGTGCATCTCGGGCTGCCCGTACAAGAAGATCTACTTCAACTGGCAGAGCGGCAAGGCC
GAGAAATGCCTGTTCTGCTATCCGCGCATCGAGGCCGGCCAGCCCACGGTCTGCTCCGAGACCTGTGTCGGCCGCATCCG
CTACCTCGGCGTGATGCTGTACGACGCGGACCGCATCGAGCAGGCCGCGTCGGTGGCCGATGAGCGCGACCTGTACCAGT
CGCAGCTCGACGTTTTCCTCGATCCGCACGATCCCGCGGTGCAAGCCGAGGCGCTGCGCCAGGGCATCCCGCAAAGCTGG
CTGGATGCCGCGCGCAAGTCGCCGGTCTACAAGATGGCGTGCGAGTGGAAGGTTGCCTTCCCGCTGCACCCGGAGTACCG
CACGCTGCCGATGGTGTGGTACATCCCGCCGCTGTCGCCGATCCAGTCGGCGGCCGAGGCCGGCCACATGGGCCTGAACG
GCGTGATCCCGGACGTGAAGAGCCTGCGCATCCCGGTCAGGTACCTGGCCAACCTGCTGACCGCGGGCGACGTGATGCCG
GTGCTGTCGGCGCTGGACCGCATGCTGGCGATGCGCGCCTACAAGCGCTCGCAGGTGGTGGACGGCGTGCAGGACCTGGG
CGTGCTGGAGCAGGTTGGCCTGACGCCCGCGCAGGTCGAGGACATGTACCAGACCATGGCCATCGCCAACTACGAGGACC
GCTTCGTGATCCCGTCCTCGCACAAGGAGATGGTCGAGGACAGCTTCAACGACAAGGCCAGTTGCGGCTTCACCTTCGGC
AACGGCTGCTCGGGCGGCACCTCGGAGGGCGCGCTGTTCGGCAAGAAGCCGCAGGGCAGCGTCCACTTTGTCGACATGCC
CAAGTCGCGCAAGAAAGCCGTGATGAGCTGA

Upstream 100 bases:

>100_bases
ACGAGTTCGTGATCCTGCGCAAGATGAAGAAGGTCGACTGGCTGGAAGGGCCGCTCGTGGAAAAGACTGCGAAGTTGACT
GTGAATGAAGGAGCCTCGCA

Downstream 100 bases:

>100_bases
GCGCGTGCCCGCATCCACGAATCGCCCGGAGAAGACCATGCCCCTCTATCCCATCCTCAGCGCCCTGTTCGGCTATCCCG
AGCAGGATCTGCTGGACGCC

Product: respiratory nitrate reductase subunit

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGVEYAWFNNVETKPGIGYPKEWENQDKWQGGWKRNADGTLEPRQGG
KLKILANLFANPNLPAIDEYYEPFTYDYEHLQKAPLVQTPPTARPVSVLTGRKMEKIEWGPNWEDDLGGEFSSRGRDKLF
DNVQKEMYSTFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREDDGIVLVDQDKCRGWRMCISGCPYKKIYFNWQSGKA
EKCLFCYPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADERDLYQSQLDVFLDPHDPAVQAEALRQGIPQSW
LDAARKSPVYKMACEWKVAFPLHPEYRTLPMVWYIPPLSPIQSAAEAGHMGLNGVIPDVKSLRIPVRYLANLLTAGDVMP
VLSALDRMLAMRAYKRSQVVDGVQDLGVLEQVGLTPAQVEDMYQTMAIANYEDRFVIPSSHKEMVEDSFNDKASCGFTFG
NGCSGGTSEGALFGKKPQGSVHFVDMPKSRKKAVMS

Sequences:

>Translated_516_residues
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGVEYAWFNNVETKPGIGYPKEWENQDKWQGGWKRNADGTLEPRQGG
KLKILANLFANPNLPAIDEYYEPFTYDYEHLQKAPLVQTPPTARPVSVLTGRKMEKIEWGPNWEDDLGGEFSSRGRDKLF
DNVQKEMYSTFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREDDGIVLVDQDKCRGWRMCISGCPYKKIYFNWQSGKA
EKCLFCYPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADERDLYQSQLDVFLDPHDPAVQAEALRQGIPQSW
LDAARKSPVYKMACEWKVAFPLHPEYRTLPMVWYIPPLSPIQSAAEAGHMGLNGVIPDVKSLRIPVRYLANLLTAGDVMP
VLSALDRMLAMRAYKRSQVVDGVQDLGVLEQVGLTPAQVEDMYQTMAIANYEDRFVIPSSHKEMVEDSFNDKASCGFTFG
NGCSGGTSEGALFGKKPQGSVHFVDMPKSRKKAVMS
>Mature_516_residues
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGVEYAWFNNVETKPGIGYPKEWENQDKWQGGWKRNADGTLEPRQGG
KLKILANLFANPNLPAIDEYYEPFTYDYEHLQKAPLVQTPPTARPVSVLTGRKMEKIEWGPNWEDDLGGEFSSRGRDKLF
DNVQKEMYSTFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREDDGIVLVDQDKCRGWRMCISGCPYKKIYFNWQSGKA
EKCLFCYPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADERDLYQSQLDVFLDPHDPAVQAEALRQGIPQSW
LDAARKSPVYKMACEWKVAFPLHPEYRTLPMVWYIPPLSPIQSAAEAGHMGLNGVIPDVKSLRIPVRYLANLLTAGDVMP
VLSALDRMLAMRAYKRSQVVDGVQDLGVLEQVGLTPAQVEDMYQTMAIANYEDRFVIPSSHKEMVEDSFNDKASCGFTFG
NGCSGGTSEGALFGKKPQGSVHFVDMPKSRKKAVMS

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=497, Percent_Identity=71.4285714285714, Blast_Score=783, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=514, Percent_Identity=68.2879377431907, Blast_Score=773, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=162, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1787122, Length=99, Percent_Identity=43.4343434343434, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI2367345, Length=101, Percent_Identity=31.6831683168317, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1787749, Length=102, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI226510944, Length=103, Percent_Identity=33.9805825242718, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789370, Length=97, Percent_Identity=35.0515463917526, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 58058; Mature: 58058

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGVEYAWFNNVETKPGIGYPKEWENQD
CCCHHEEEEEEEHHHHHCCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWQGGWKRNADGTLEPRQGGKLKILANLFANPNLPAIDEYYEPFTYDYEHLQKAPLVQTP
HHCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCHHHHCCCCCCCHHHHCCCCCCCCC
PTARPVSVLTGRKMEKIEWGPNWEDDLGGEFSSRGRDKLFDNVQKEMYSTFENTFMMYLP
CCCCCHHHHCCCCHHCCCCCCCCCHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVASCPSGSIYKREDDGIVLVDQDKCRGWRMCISGCPYKKIYFNWQSGKA
HHHHHHCCCCEEECCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCLFCYPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADERDLYQSQLDVFL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHEEE
DPHDPAVQAEALRQGIPQSWLDAARKSPVYKMACEWKVAFPLHPEYRTLPMVWYIPPLSP
CCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCEEEEECCCHH
IQSAAEAGHMGLNGVIPDVKSLRIPVRYLANLLTAGDVMPVLSALDRMLAMRAYKRSQVV
HHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
DGVQDLGVLEQVGLTPAQVEDMYQTMAIANYEDRFVIPSSHKEMVEDSFNDKASCGFTFG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHCCCCCCCCCEEEC
NGCSGGTSEGALFGKKPQGSVHFVDMPKSRKKAVMS
CCCCCCCCCCCEECCCCCCCEEEEECCCHHHHHCCC
>Mature Secondary Structure
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGVEYAWFNNVETKPGIGYPKEWENQD
CCCHHEEEEEEEHHHHHCCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWQGGWKRNADGTLEPRQGGKLKILANLFANPNLPAIDEYYEPFTYDYEHLQKAPLVQTP
HHCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCHHHHCCCCCCCHHHHCCCCCCCCC
PTARPVSVLTGRKMEKIEWGPNWEDDLGGEFSSRGRDKLFDNVQKEMYSTFENTFMMYLP
CCCCCHHHHCCCCHHCCCCCCCCCHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVASCPSGSIYKREDDGIVLVDQDKCRGWRMCISGCPYKKIYFNWQSGKA
HHHHHHCCCCEEECCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCLFCYPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADERDLYQSQLDVFL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHEEE
DPHDPAVQAEALRQGIPQSWLDAARKSPVYKMACEWKVAFPLHPEYRTLPMVWYIPPLSP
CCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCEEEEECCCHH
IQSAAEAGHMGLNGVIPDVKSLRIPVRYLANLLTAGDVMPVLSALDRMLAMRAYKRSQVV
HHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
DGVQDLGVLEQVGLTPAQVEDMYQTMAIANYEDRFVIPSSHKEMVEDSFNDKASCGFTFG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHCCCCCCCCCEEEC
NGCSGGTSEGALFGKKPQGSVHFVDMPKSRKKAVMS
CCCCCCCCCCCEECCCCCCCEEEEECCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]