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The map label for this gene is mraW

Identifier: 38234181

GI number: 38234181

Start: 1638906

End: 1639925

Strand: Reverse

Name: mraW

Synonym: DIP1606

Alternate gene names: 38234181

Gene position: 1639925-1638906 (Counterclockwise)

Preceding gene: 38234182

Following gene: 38234180

Centisome position: 65.9

GC content: 49.8

Gene sequence:

>1020_bases
ATGACTCACGGTGAGCCGCAAGAGTTTTCGTTCGATGTTGAGGAAAACCATGGTCACGTCCCAGTTCTTCGAGCCAGAAT
GGCTGAGCTGATTGCGCCTAAGGTTACAGCGATGGGGTCAGATGCTGTCATTATTGATGGCACTCTTGGCGCTGGTGGGC
ACAGCAAGTATTTTCTTGAAAGCTTCCCCGAAGCTCGAGTTATAGGCCTCGATCGTGATACTAACTCTTTAGCTTCGGCG
CGCGAACGACTAAAAGAATTTGGTGATCGATTCCTCGGTATTCACACCCGTTTCGATCGATTTGCCCCGAAATTAGAGGA
ACTGGCTCAATCTGGAAACCCCATTGCAGCTACTGCATTGGAGCAAGGTATCAGCGGTGCACTTTTCGACCTCGGGGTTT
CATCAATGCAGCTTGATCAAGCAGAGCGAGGATTTGCCTACCGTGTTGATGCGCCACTAGATATGCGCATGGATAACACG
ATCGGGATCACTGCAGCTGATGTGTTGAATACCTATAGCCATGGCGAGCTAGCGCGCATCCTTAAAACCTATGGTGACGA
GCGTTTCGCAGGAAAAATTGCAAGTGCAATAGTCCGAGAGCGTGAAAAAGAACCATTTGACACTTCGGCTCGTCTAGTTG
AGCTGCTTTACGCGACGATTCCTGCAGCTACGCGTCGTACTGGTGGGCATCCAGCCAAGCGAACATTCCAAGCGCTTCGG
ATCGAAGTCAATCGAGAATTGGAATCTCTAGAGAATGTACTTCCTGAGATTACGCAGCGTTTGACTACAGGTGGACGAGC
AGTGTTCATGTCGTACCAGTCGTTGGAAGACAAAATTGTCAAACGTTACTTCGTAGACATTTCTACTTCGAAAACGCCGC
CGGGGCTTCCCATGGAGCTACCGGAATATGCGCCCAAATTCAAAGTAGTGACCCGTGGTGCAGAAAAAGCTACTGACGCG
GAAATCGAGGAAAATCCTCGCGCTGCATCAGTAAGAGTTCGAGCAATCGAAGCTCTGTAG

Upstream 100 bases:

>100_bases
AGCGTACCAGTCAGGGCTCTGTGTAGACCCCGCCTTTACTACCTAAAGACCCAACAACATGTGTCTCAGGGCATGTGAAT
AAGCTCGAGAAAGGGGATAA

Downstream 100 bases:

>100_bases
AGAAATCAAAAACGCAATGACACAAACAAACGCAGTAGTTTTGGGGTGACCTAGAGATGACCATGACCGACCGTGCGCGT
AGGGAGGCCAGCCACCGTTC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTHGEPQEFSFDVEENHGHVPVLRARMAELIAPKVTAMGSDAVIIDGTLGAGGHSKYFLESFPEARVIGLDRDTNSLASA
RERLKEFGDRFLGIHTRFDRFAPKLEELAQSGNPIAATALEQGISGALFDLGVSSMQLDQAERGFAYRVDAPLDMRMDNT
IGITAADVLNTYSHGELARILKTYGDERFAGKIASAIVREREKEPFDTSARLVELLYATIPAATRRTGGHPAKRTFQALR
IEVNRELESLENVLPEITQRLTTGGRAVFMSYQSLEDKIVKRYFVDISTSKTPPGLPMELPEYAPKFKVVTRGAEKATDA
EIEENPRAASVRVRAIEAL

Sequences:

>Translated_339_residues
MTHGEPQEFSFDVEENHGHVPVLRARMAELIAPKVTAMGSDAVIIDGTLGAGGHSKYFLESFPEARVIGLDRDTNSLASA
RERLKEFGDRFLGIHTRFDRFAPKLEELAQSGNPIAATALEQGISGALFDLGVSSMQLDQAERGFAYRVDAPLDMRMDNT
IGITAADVLNTYSHGELARILKTYGDERFAGKIASAIVREREKEPFDTSARLVELLYATIPAATRRTGGHPAKRTFQALR
IEVNRELESLENVLPEITQRLTTGGRAVFMSYQSLEDKIVKRYFVDISTSKTPPGLPMELPEYAPKFKVVTRGAEKATDA
EIEENPRAASVRVRAIEAL
>Mature_338_residues
THGEPQEFSFDVEENHGHVPVLRARMAELIAPKVTAMGSDAVIIDGTLGAGGHSKYFLESFPEARVIGLDRDTNSLASAR
ERLKEFGDRFLGIHTRFDRFAPKLEELAQSGNPIAATALEQGISGALFDLGVSSMQLDQAERGFAYRVDAPLDMRMDNTI
GITAADVLNTYSHGELARILKTYGDERFAGKIASAIVREREKEPFDTSARLVELLYATIPAATRRTGGHPAKRTFQALRI
EVNRELESLENVLPEITQRLTTGGRAVFMSYQSLEDKIVKRYFVDISTSKTPPGLPMELPEYAPKFKVVTRGAEKATDAE
IEENPRAASVRVRAIEAL

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=347, Percent_Identity=34.2939481268012, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI165377202, Length=202, Percent_Identity=36.1386138613861, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1786270, Length=303, Percent_Identity=41.5841584158416, Blast_Score=192, Evalue=3e-50,
Organism=Drosophila melanogaster, GI62472493, Length=339, Percent_Identity=33.3333333333333, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI28571637, Length=339, Percent_Identity=33.3333333333333, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_CORDI (P60395)

Other databases:

- EMBL:   BX248358
- RefSeq:   NP_939948.1
- ProteinModelPortal:   P60395
- SMR:   P60395
- GeneID:   2650795
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1606
- NMPDR:   fig|257309.1.peg.1539
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   CDIP257309:DIP1606-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 37409; Mature: 37278

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: BINDING 71-71 BINDING 98-98 BINDING 130-130 BINDING 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHGEPQEFSFDVEENHGHVPVLRARMAELIAPKVTAMGSDAVIIDGTLGAGGHSKYFLE
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHCCCCCEEEEECCCCCCCCCCHHHH
SFPEARVIGLDRDTNSLASARERLKEFGDRFLGIHTRFDRFAPKLEELAQSGNPIAATAL
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCEEHHHH
EQGISGALFDLGVSSMQLDQAERGFAYRVDAPLDMRMDNTIGITAADVLNTYSHGELARI
HHCCCCHHHHHCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCEEHHHHHHHCCCHHHHHH
LKTYGDERFAGKIASAIVREREKEPFDTSARLVELLYATIPAATRRTGGHPAKRTFQALR
HHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHH
IEVNRELESLENVLPEITQRLTTGGRAVFMSYQSLEDKIVKRYFVDISTSKTPPGLPMEL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PEYAPKFKVVTRGAEKATDAEIEENPRAASVRVRAIEAL
HHCCCCEEEEECCCCCCCCCCHHCCCCHHHEEEEEEECC
>Mature Secondary Structure 
THGEPQEFSFDVEENHGHVPVLRARMAELIAPKVTAMGSDAVIIDGTLGAGGHSKYFLE
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHCCCCCEEEEECCCCCCCCCCHHHH
SFPEARVIGLDRDTNSLASARERLKEFGDRFLGIHTRFDRFAPKLEELAQSGNPIAATAL
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCEEHHHH
EQGISGALFDLGVSSMQLDQAERGFAYRVDAPLDMRMDNTIGITAADVLNTYSHGELARI
HHCCCCHHHHHCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCEEHHHHHHHCCCHHHHHH
LKTYGDERFAGKIASAIVREREKEPFDTSARLVELLYATIPAATRRTGGHPAKRTFQALR
HHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHH
IEVNRELESLENVLPEITQRLTTGGRAVFMSYQSLEDKIVKRYFVDISTSKTPPGLPMEL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PEYAPKFKVVTRGAEKATDAEIEENPRAASVRVRAIEAL
HHCCCCEEEEECCCCCCCCCCHHCCCCHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910