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The map label for this gene is ilvA [H]

Identifier: 38234154

GI number: 38234154

Start: 1604995

End: 1606335

Strand: Reverse

Name: ilvA [H]

Synonym: DIP1579

Alternate gene names: 38234154

Gene position: 1606335-1604995 (Counterclockwise)

Preceding gene: 38234155

Following gene: 38234153

Centisome position: 64.55

GC content: 52.05

Gene sequence:

>1341_bases
GTGTTAGGAAGCAGTAGTACCCCGTGGTTAAAATTGTTGCCCATGAGTAAAGAAGCAGAAAACCAGCAGGTCACTTTCGG
CGCAATTCACGCCGCTGATATTCAAATGGCACAGTCTCGAATTTCTGCTTCTATTGCACCGACGCCCCTGCAGCATTGCC
CACGGTTGTCGCAAATGACGGGATGCGAAGTCTATCTCAAGCGCGAAGACCTTCAAGACGTTCGGTCCTATAAAATCCGT
GGTGCTGTCAATGCGATCTCGCAACTGACAGAGGAACAACGAGTGGCTGGGATCGTGGCGGCGTCGGCAGGCAATCATGC
TCAAGGTGTGGCTTTCGCCTGCCGAACCATGGGTATCAAAGGTCGAATCTACGTTCCGGCAGCAACGCCAAAGCAAAAAC
GCGACCGTATCATGGTCCATGGTGGCGACGCTGTAGAACTCGTAGTCACCGGTGCCAATTTTGATGAAGCGGCAGCTGCT
GCACGTGAGTACGCTCGCGTTGAAGGCGCAACGATGGTGGAACCATTCGATAACCGCGATACCGTTATCGGCCAAGGAAC
TGTAGCAGCAGAGATCTTGGCACAATTAACAACGCTGGGTAAAACACTTGATACCGTCGTCGTGCCCGTTGGTGGTGGTG
GATTGCTTTCCGGCGTTGTCAGCTATCTTGCTGATATGGCGCCACGCACCGCGATCGTTGGAGTAGAACCAGAAGGTGCG
GCATCCATGCGCGCCGCTCTGGATGCGCACGGTCCTGTGACCTTGGATAATGTTGACCCATTTGTCGACGGTGCCGCTGT
GAAACGTATCGGTGATCTTAACTATCACATCATCGAACACAACCAAGGCCGTATTCACCCCATCAGCGTTAATGAAGGTG
CTGTATGTACTGAGATGCTTGACTTGTATCAAAACGAGGGCATCATTACAGAACCTGCAGGGGCGCTGTCAGTTACTGGA
TTGAAAGAGCTGTCACTTGGAAGCAGGGACGTAGTTGTCTGCATCATCTCTGGTGGTAACAACGATGTGCTGCGTTATGG
GGAAATCATGGAGCGCTCCCTCGTTCATCGCGGACTCAAGCACTACTTCCTCGTCAATTTCCCACAGGAGCCAGGGCAGC
TACGTACTTTCCTGAACGACATCTTGGGCCCAGATGACGACATCACTTTGTTTGAATATCTCAAACGCAACAACCGTGAA
ACCGGAACAGCGCTCGTAGGACTTCAACTCGGAGTTGCTTCCGAACTTGACGGTTTGCTCGAGCGAATGAATGCTTCGCG
GATTGATTGCCGGCGACTAGAACCTGGAACGCCAGAGTATAGTTACCTCGTTAATTTGTAA

Upstream 100 bases:

>100_bases
AAAAACAGTGATGATTTTGGGCGAACACCTCCGAGTTAATCGCAGTGGAAATCTCATGGGTGACCTGAAAGCCACCATGG
GGCCGGGCATTTTGGGGTAG

Downstream 100 bases:

>100_bases
CGACTCGGACGAAAAGGAGAGCGGTGGACCTCTACCGTAGGCCTGTTGCCCACGAGGAATTTCGCAACGAAATCGAGATT
AAACGGTCACGATTCATCAC

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MLGSSSTPWLKLLPMSKEAENQQVTFGAIHAADIQMAQSRISASIAPTPLQHCPRLSQMTGCEVYLKREDLQDVRSYKIR
GAVNAISQLTEEQRVAGIVAASAGNHAQGVAFACRTMGIKGRIYVPAATPKQKRDRIMVHGGDAVELVVTGANFDEAAAA
AREYARVEGATMVEPFDNRDTVIGQGTVAAEILAQLTTLGKTLDTVVVPVGGGGLLSGVVSYLADMAPRTAIVGVEPEGA
ASMRAALDAHGPVTLDNVDPFVDGAAVKRIGDLNYHIIEHNQGRIHPISVNEGAVCTEMLDLYQNEGIITEPAGALSVTG
LKELSLGSRDVVVCIISGGNNDVLRYGEIMERSLVHRGLKHYFLVNFPQEPGQLRTFLNDILGPDDDITLFEYLKRNNRE
TGTALVGLQLGVASELDGLLERMNASRIDCRRLEPGTPEYSYLVNL

Sequences:

>Translated_446_residues
MLGSSSTPWLKLLPMSKEAENQQVTFGAIHAADIQMAQSRISASIAPTPLQHCPRLSQMTGCEVYLKREDLQDVRSYKIR
GAVNAISQLTEEQRVAGIVAASAGNHAQGVAFACRTMGIKGRIYVPAATPKQKRDRIMVHGGDAVELVVTGANFDEAAAA
AREYARVEGATMVEPFDNRDTVIGQGTVAAEILAQLTTLGKTLDTVVVPVGGGGLLSGVVSYLADMAPRTAIVGVEPEGA
ASMRAALDAHGPVTLDNVDPFVDGAAVKRIGDLNYHIIEHNQGRIHPISVNEGAVCTEMLDLYQNEGIITEPAGALSVTG
LKELSLGSRDVVVCIISGGNNDVLRYGEIMERSLVHRGLKHYFLVNFPQEPGQLRTFLNDILGPDDDITLFEYLKRNNRE
TGTALVGLQLGVASELDGLLERMNASRIDCRRLEPGTPEYSYLVNL
>Mature_446_residues
MLGSSSTPWLKLLPMSKEAENQQVTFGAIHAADIQMAQSRISASIAPTPLQHCPRLSQMTGCEVYLKREDLQDVRSYKIR
GAVNAISQLTEEQRVAGIVAASAGNHAQGVAFACRTMGIKGRIYVPAATPKQKRDRIMVHGGDAVELVVTGANFDEAAAA
AREYARVEGATMVEPFDNRDTVIGQGTVAAEILAQLTTLGKTLDTVVVPVGGGGLLSGVVSYLADMAPRTAIVGVEPEGA
ASMRAALDAHGPVTLDNVDPFVDGAAVKRIGDLNYHIIEHNQGRIHPISVNEGAVCTEMLDLYQNEGIITEPAGALSVTG
LKELSLGSRDVVVCIISGGNNDVLRYGEIMERSLVHRGLKHYFLVNFPQEPGQLRTFLNDILGPDDDITLFEYLKRNNRE
TGTALVGLQLGVASELDGLLERMNASRIDCRRLEPGTPEYSYLVNL

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia. Deaminates L-threonine, but also L-serine to a lesser extent [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=326, Percent_Identity=30.0613496932515, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI19923959, Length=307, Percent_Identity=28.6644951140065, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1790207, Length=370, Percent_Identity=39.1891891891892, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1789505, Length=320, Percent_Identity=34.0625, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1789235, Length=356, Percent_Identity=24.7191011235955, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17537387, Length=373, Percent_Identity=31.6353887399464, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI71991565, Length=328, Percent_Identity=31.0975609756098, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17508781, Length=287, Percent_Identity=28.2229965156794, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320930, Length=403, Percent_Identity=34.2431761786601, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6322631, Length=320, Percent_Identity=30.9375, Blast_Score=139, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6319788, Length=308, Percent_Identity=25.974025974026, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355833, Length=366, Percent_Identity=32.2404371584699, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24645328, Length=253, Percent_Identity=31.6205533596838, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR001721
- InterPro:   IPR011820 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 47912; Mature: 47912

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGSSSTPWLKLLPMSKEAENQQVTFGAIHAADIQMAQSRISASIAPTPLQHCPRLSQMT
CCCCCCCCEEEEEECCCCCCCCEEEEEEEEHHHHHHHHHHHHCCCCCCCHHHCCCHHHCC
GCEVYLKREDLQDVRSYKIRGAVNAISQLTEEQRVAGIVAASAGNHAQGVAFACRTMGIK
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHCCCC
GRIYVPAATPKQKRDRIMVHGGDAVELVVTGANFDEAAAAAREYARVEGATMVEPFDNRD
CEEEECCCCCHHHCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCCC
TVIGQGTVAAEILAQLTTLGKTLDTVVVPVGGGGLLSGVVSYLADMAPRTAIVGVEPEGA
CEEECCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCC
ASMRAALDAHGPVTLDNVDPFVDGAAVKRIGDLNYHIIEHNQGRIHPISVNEGAVCTEML
HHHHHHHCCCCCEEECCCCCCCCCHHHHHHCCCEEEEEECCCCEEEEEECCCCHHHHHHH
DLYQNEGIITEPAGALSVTGLKELSLGSRDVVVCIISGGNNDVLRYGEIMERSLVHRGLK
HHHHCCCCEECCCCCEEECCHHHHCCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHCCC
HYFLVNFPQEPGQLRTFLNDILGPDDDITLFEYLKRNNRETGTALVGLQLGVASELDGLL
EEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHH
ERMNASRIDCRRLEPGTPEYSYLVNL
HHHCCCCCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MLGSSSTPWLKLLPMSKEAENQQVTFGAIHAADIQMAQSRISASIAPTPLQHCPRLSQMT
CCCCCCCCEEEEEECCCCCCCCEEEEEEEEHHHHHHHHHHHHCCCCCCCHHHCCCHHHCC
GCEVYLKREDLQDVRSYKIRGAVNAISQLTEEQRVAGIVAASAGNHAQGVAFACRTMGIK
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHCCCC
GRIYVPAATPKQKRDRIMVHGGDAVELVVTGANFDEAAAAAREYARVEGATMVEPFDNRD
CEEEECCCCCHHHCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCCC
TVIGQGTVAAEILAQLTTLGKTLDTVVVPVGGGGLLSGVVSYLADMAPRTAIVGVEPEGA
CEEECCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCC
ASMRAALDAHGPVTLDNVDPFVDGAAVKRIGDLNYHIIEHNQGRIHPISVNEGAVCTEML
HHHHHHHCCCCCEEECCCCCCCCCHHHHHHCCCEEEEEECCCCEEEEEECCCCHHHHHHH
DLYQNEGIITEPAGALSVTGLKELSLGSRDVVVCIISGGNNDVLRYGEIMERSLVHRGLK
HHHHCCCCEECCCCCEEECCHHHHCCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHCCC
HYFLVNFPQEPGQLRTFLNDILGPDDDITLFEYLKRNNRETGTALVGLQLGVASELDGLL
EEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHH
ERMNASRIDCRRLEPGTPEYSYLVNL
HHHCCCCCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1459955; 12948626 [H]