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The map label for this gene is hisH

Identifier: 38234136

GI number: 38234136

Start: 1588634

End: 1589266

Strand: Reverse

Name: hisH

Synonym: DIP1561

Alternate gene names: 38234136

Gene position: 1589266-1588634 (Counterclockwise)

Preceding gene: 38234137

Following gene: 38234135

Centisome position: 63.86

GC content: 52.45

Gene sequence:

>633_bases
ATGAAGAAAGTGGCACTTCTCGATTACGGTTCTGGGAATCTACGTTCAGCGCAACGAGCGTTGGAACGAGTTGGCGCAGA
AGTAGAGGTAACAAACGATCCGGATGTAGTACTGGCTGCGGACGGTCTCCTCGTACCAGGAGTGGGTGCTTTTGATGCAT
GCATGAAGGGGCTAAAAGCGGTCCAAGGTGACCGAATGATTGGTCAGCGCTTGGCAGGGGGACGCCCTGTGATGGGCATT
TGTGTAGGTATGCAGATCATGTTTGATTCTGGCAACGAACACGGCATCTCTGCAGCTGGATGTGGGCAGTGGCCAGGAAA
CGTCGAAAAGCTTGAGGCTCGTGTTCTACCGCATATGGGTTGGAACACGGTGGAAGCAGCGCAAGACTCACAACTTTTTG
CTGGACTCGACGCTGATACGCGTTTTTATTTTGTGCACTCTTACGGTGTGCGTTGGTGGGAGTTCGAAGGCGACGGTTTA
ACACGTGCACCATTAGTAACGTGGGCTCAGCATGAATCTGACCGATTTGTTGCCGCTGTGGAAAACGGTGCGTTGATGGC
TACCCAGTTCCACCCAGAGAAATCTGGGGATGCAGGTGCACAACTCCTGCGGAATTGGATTGATCTGCTGTAA

Upstream 100 bases:

>100_bases
TGCGCGTCGTGTAGCGGCGTCTGTTGCAGATTAACGTGTGGGGGTAGTTCGGCACGAGCACCCCGCAATGGTGGTTGATA
ACCAGTTAGGATGAGAAACC

Downstream 100 bases:

>100_bases
AACCCGCAATGCGGGACAGCTCGAGGATTCGGTGGGGTAGGGCTTCAACCCCGCTAGGGTGAGCCTTGTTGCGGATAAGC
GTCTACACTATTGCTTTATG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKKVALLDYGSGNLRSAQRALERVGAEVEVTNDPDVVLAADGLLVPGVGAFDACMKGLKAVQGDRMIGQRLAGGRPVMGI
CVGMQIMFDSGNEHGISAAGCGQWPGNVEKLEARVLPHMGWNTVEAAQDSQLFAGLDADTRFYFVHSYGVRWWEFEGDGL
TRAPLVTWAQHESDRFVAAVENGALMATQFHPEKSGDAGAQLLRNWIDLL

Sequences:

>Translated_210_residues
MKKVALLDYGSGNLRSAQRALERVGAEVEVTNDPDVVLAADGLLVPGVGAFDACMKGLKAVQGDRMIGQRLAGGRPVMGI
CVGMQIMFDSGNEHGISAAGCGQWPGNVEKLEARVLPHMGWNTVEAAQDSQLFAGLDADTRFYFVHSYGVRWWEFEGDGL
TRAPLVTWAQHESDRFVAAVENGALMATQFHPEKSGDAGAQLLRNWIDLL
>Mature_210_residues
MKKVALLDYGSGNLRSAQRALERVGAEVEVTNDPDVVLAADGLLVPGVGAFDACMKGLKAVQGDRMIGQRLAGGRPVMGI
CVGMQIMFDSGNEHGISAAGCGQWPGNVEKLEARVLPHMGWNTVEAAQDSQLFAGLDADTRFYFVHSYGVRWWEFEGDGL
TRAPLVTWAQHESDRFVAAVENGALMATQFHPEKSGDAGAQLLRNWIDLL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=213, Percent_Identity=37.0892018779343, Blast_Score=125, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=216, Percent_Identity=32.4074074074074, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_CORDI (P60598)

Other databases:

- EMBL:   BX248358
- RefSeq:   NP_939903.1
- ProteinModelPortal:   P60598
- SMR:   P60598
- GeneID:   2650750
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1561
- NMPDR:   fig|257309.1.peg.1494
- HOGENOM:   HBG292341
- OMA:   SVRFAFE
- PhylomeDB:   P60598
- ProtClustDB:   PRK13146
- BioCyc:   CDIP257309:DIP1561-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22687; Mature: 22687

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 81-81 ACT_SITE 191-191 ACT_SITE 193-193

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVALLDYGSGNLRSAQRALERVGAEVEVTNDPDVVLAADGLLVPGVGAFDACMKGLKA
CCEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCEEEEECCEEECCCCHHHHHHHHHHH
VQGDRMIGQRLAGGRPVMGICVGMQIMFDSGNEHGISAAGCGQWPGNVEKLEARVLPHMG
HCCCHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
WNTVEAAQDSQLFAGLDADTRFYFVHSYGVRWWEFEGDGLTRAPLVTWAQHESDRFVAAV
CCCHHHCCCCCEEECCCCCCEEEEEEECCCEEEEECCCCCCCCCEEEEECCCCCCEEEEE
ENGALMATQFHPEKSGDAGAQLLRNWIDLL
CCCEEEEEEECCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKVALLDYGSGNLRSAQRALERVGAEVEVTNDPDVVLAADGLLVPGVGAFDACMKGLKA
CCEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCEEEEECCEEECCCCHHHHHHHHHHH
VQGDRMIGQRLAGGRPVMGICVGMQIMFDSGNEHGISAAGCGQWPGNVEKLEARVLPHMG
HCCCHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
WNTVEAAQDSQLFAGLDADTRFYFVHSYGVRWWEFEGDGLTRAPLVTWAQHESDRFVAAV
CCCHHHCCCCCEEECCCCCCEEEEEEECCCEEEEECCCCCCCCCEEEEECCCCCCEEEEE
ENGALMATQFHPEKSGDAGAQLLRNWIDLL
CCCEEEEEEECCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910