Click here to switch to the map view.

The map label for this gene is pyk [H]

Identifier: 38234128

GI number: 38234128

Start: 1581632

End: 1583053

Strand: Reverse

Name: pyk [H]

Synonym: DIP1553

Alternate gene names: 38234128

Gene position: 1583053-1581632 (Counterclockwise)

Preceding gene: 38234129

Following gene: 38234124

Centisome position: 63.61

GC content: 51.83

Gene sequence:

>1422_bases
ATGGAACGTCGAACTAAGATTGTTTGTACATTAGGGCCGGCAGTCGCTTCCCGTGAGGGAATTCTTGGACTGGTTAAAGC
CGGCATGAATGTCGCACGTATGAATATGTCACACGGCGACCACGCAGATCATGAGAAGAATTACCAGTGGGTCCGCGAAG
CTACGGATGAAACCGGACGCGCTGTTGGTATTCTTGCCGATCTGCAGGGGCCAAAGATTCGTCTCGGTCGCTTTATCGAT
GGCGCAACGGTGTGGGAGAACGGTGAAATCGTTCGTATCACTGTTGATGACATTGAAGGTACACACGACCGTGTGTCTAC
CACCTACAAGAACCTCGCTAAAGATGCGAAGCCTGGCGATCGTCTTTTGGTCGACGACGGCAAGGTTGCATTGCGCTGTG
TTGAAGTAGATGGAAACGATGTTGTTTGTGAGGTTGTAGAAGGCGGCCCAGTCTCTAACAACAAGGGTGTTTCCCTTCCT
GGAATGGATATTTCCGTTCCAGCTTTGTCTGAAAAAGACATTGCTGATTTGCGCTTCGCTCTCAATCTCGGTGTTGACAT
TATTGCGTTGTCCTTCGTCCGCTCCCCAGCAGACGTGGAACTTGTTCATGCCATCATGGATGAAGAAGGTCGCCGTTGCC
CAGTGATCGCAAAGCTTGAGAAGCCAGAAGCCATGGACGCTTTGGAGTCCATCGTATTGGCTTTCGACGGCATCATGATT
GCTCGTGGTGACTTGGGTGTTGAGTGCCCGTTGGAGCAGGTGCCGCTGTTCCAGAAGCGTGCCATCCAGATTGCTCGTGA
AAACGCTAAGCCTGTGATTGTGGCAACCCAGATGCTCGATTCCATGATCGAGAACCTACGCCCAACCCGTGCTGAGGCTT
CTGACGTAGCTAACGCTGTTCTTGACGGTGCAGATGCCGTTATGCTGTCCGGTGAGACTTCGGTCGGTATCGATCCAGCT
AACGTTGTCCGTACTATGTCACGTATCGTTTCCTACGCTGAGATTGATGGCCGGGTACCAAACTTGGCTCATATTCCACG
CACCAAGCGTGGCGTGATTTCCTACTCTGCACGCGACATTGCAGAGCGTTTGAATGCAAAGGCACTTGTAGCATTCACCT
CTTCAGGTGATACCGCAAAGCGTGTTGCTCGTTTGCACTCGCAGCTGCCGCTATTGGTCTTTACCCCTCACCAAGAAGTC
CGCTCTCAGTTGGCTTTGACTTGGGGTGTAGAAACCTTCCTTACCGACGAGGTTAAGGACACAGACGAGATGATGCGTAC
GATCGATGAGCAGTTGCTTGCTCTCGATACCTACAGCGAAGATGACATGATCGTGCTCGTAGCCGGAACCCCTCCAGGAG
TCCAAGGCAATACCAACATGATCCATGTACACCACCTAGGTGAGGACCTCAACGGTCTATAA

Upstream 100 bases:

>100_bases
TGTGATTTTGGCGTGTGATGGCGCCCAACTGGCTATTTAGTTGCCTTTCACACGGTCCGATTCCTAGCGGTGATCCGCTA
ACAGGGTTAGTGTTTAGGGC

Downstream 100 bases:

>100_bases
ATCTTGGATAATAAAAGACCACCGTGGTCAGGGGATAAACCTTTCCACGGTGGATTTTTTATGCACTAGTGGTTAATCGG
CGTTGCGTCGATCTTCCATA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MERRTKIVCTLGPAVASREGILGLVKAGMNVARMNMSHGDHADHEKNYQWVREATDETGRAVGILADLQGPKIRLGRFID
GATVWENGEIVRITVDDIEGTHDRVSTTYKNLAKDAKPGDRLLVDDGKVALRCVEVDGNDVVCEVVEGGPVSNNKGVSLP
GMDISVPALSEKDIADLRFALNLGVDIIALSFVRSPADVELVHAIMDEEGRRCPVIAKLEKPEAMDALESIVLAFDGIMI
ARGDLGVECPLEQVPLFQKRAIQIARENAKPVIVATQMLDSMIENLRPTRAEASDVANAVLDGADAVMLSGETSVGIDPA
NVVRTMSRIVSYAEIDGRVPNLAHIPRTKRGVISYSARDIAERLNAKALVAFTSSGDTAKRVARLHSQLPLLVFTPHQEV
RSQLALTWGVETFLTDEVKDTDEMMRTIDEQLLALDTYSEDDMIVLVAGTPPGVQGNTNMIHVHHLGEDLNGL

Sequences:

>Translated_473_residues
MERRTKIVCTLGPAVASREGILGLVKAGMNVARMNMSHGDHADHEKNYQWVREATDETGRAVGILADLQGPKIRLGRFID
GATVWENGEIVRITVDDIEGTHDRVSTTYKNLAKDAKPGDRLLVDDGKVALRCVEVDGNDVVCEVVEGGPVSNNKGVSLP
GMDISVPALSEKDIADLRFALNLGVDIIALSFVRSPADVELVHAIMDEEGRRCPVIAKLEKPEAMDALESIVLAFDGIMI
ARGDLGVECPLEQVPLFQKRAIQIARENAKPVIVATQMLDSMIENLRPTRAEASDVANAVLDGADAVMLSGETSVGIDPA
NVVRTMSRIVSYAEIDGRVPNLAHIPRTKRGVISYSARDIAERLNAKALVAFTSSGDTAKRVARLHSQLPLLVFTPHQEV
RSQLALTWGVETFLTDEVKDTDEMMRTIDEQLLALDTYSEDDMIVLVAGTPPGVQGNTNMIHVHHLGEDLNGL
>Mature_473_residues
MERRTKIVCTLGPAVASREGILGLVKAGMNVARMNMSHGDHADHEKNYQWVREATDETGRAVGILADLQGPKIRLGRFID
GATVWENGEIVRITVDDIEGTHDRVSTTYKNLAKDAKPGDRLLVDDGKVALRCVEVDGNDVVCEVVEGGPVSNNKGVSLP
GMDISVPALSEKDIADLRFALNLGVDIIALSFVRSPADVELVHAIMDEEGRRCPVIAKLEKPEAMDALESIVLAFDGIMI
ARGDLGVECPLEQVPLFQKRAIQIARENAKPVIVATQMLDSMIENLRPTRAEASDVANAVLDGADAVMLSGETSVGIDPA
NVVRTMSRIVSYAEIDGRVPNLAHIPRTKRGVISYSARDIAERLNAKALVAFTSSGDTAKRVARLHSQLPLLVFTPHQEV
RSQLALTWGVETFLTDEVKDTDEMMRTIDEQLLALDTYSEDDMIVLVAGTPPGVQGNTNMIHVHHLGEDLNGL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=487, Percent_Identity=36.9609856262834, Blast_Score=281, Evalue=8e-76,
Organism=Homo sapiens, GI33286422, Length=488, Percent_Identity=36.8852459016393, Blast_Score=278, Evalue=8e-75,
Organism=Homo sapiens, GI33286420, Length=488, Percent_Identity=36.8852459016393, Blast_Score=278, Evalue=8e-75,
Organism=Homo sapiens, GI32967597, Length=487, Percent_Identity=36.7556468172485, Blast_Score=268, Evalue=6e-72,
Organism=Homo sapiens, GI10835121, Length=487, Percent_Identity=36.7556468172485, Blast_Score=268, Evalue=7e-72,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=39.5833333333333, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=39.5833333333333, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=37.2881355932203, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=37.2881355932203, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=36.2790697674419, Blast_Score=135, Evalue=6e-32,
Organism=Escherichia coli, GI1787965, Length=472, Percent_Identity=39.8305084745763, Blast_Score=314, Evalue=6e-87,
Organism=Escherichia coli, GI1788160, Length=432, Percent_Identity=41.6666666666667, Blast_Score=288, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI17544584, Length=422, Percent_Identity=37.2037914691943, Blast_Score=283, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI71984413, Length=424, Percent_Identity=40.8018867924528, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI17506829, Length=424, Percent_Identity=40.8018867924528, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI71984406, Length=424, Percent_Identity=40.8018867924528, Blast_Score=265, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI17506831, Length=424, Percent_Identity=40.8018867924528, Blast_Score=265, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6324923, Length=492, Percent_Identity=33.130081300813, Blast_Score=253, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6319279, Length=480, Percent_Identity=35, Blast_Score=251, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24648964, Length=487, Percent_Identity=38.1930184804928, Blast_Score=281, Evalue=7e-76,
Organism=Drosophila melanogaster, GI28571814, Length=487, Percent_Identity=38.1930184804928, Blast_Score=281, Evalue=9e-76,
Organism=Drosophila melanogaster, GI24581235, Length=484, Percent_Identity=32.0247933884297, Blast_Score=241, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24648966, Length=414, Percent_Identity=35.7487922705314, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24646914, Length=291, Percent_Identity=35.0515463917526, Blast_Score=140, Evalue=3e-33,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51338; Mature: 51338

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERRTKIVCTLGPAVASREGILGLVKAGMNVARMNMSHGDHADHEKNYQWVREATDETGR
CCCCEEEEEEECCHHHCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCC
AVGILADLQGPKIRLGRFIDGATVWENGEIVRITVDDIEGTHDRVSTTYKNLAKDAKPGD
EEEEEEECCCCCEEECEEECCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCC
RLLVDDGKVALRCVEVDGNDVVCEVVEGGPVSNNKGVSLPGMDISVPALSEKDIADLRFA
EEEEECCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHH
LNLGVDIIALSFVRSPADVELVHAIMDEEGRRCPVIAKLEKPEAMDALESIVLAFDGIMI
HHCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCEEE
ARGDLGVECPLEQVPLFQKRAIQIARENAKPVIVATQMLDSMIENLRPTRAEASDVANAV
EECCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHH
LDGADAVMLSGETSVGIDPANVVRTMSRIVSYAEIDGRVPNLAHIPRTKRGVISYSARDI
HCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHH
AERLNAKALVAFTSSGDTAKRVARLHSQLPLLVFTPHQEVRSQLALTWGVETFLTDEVKD
HHHCCCEEEEEEECCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCHHHHHCCCCCC
TDEMMRTIDEQLLALDTYSEDDMIVLVAGTPPGVQGNTNMIHVHHLGEDLNGL
HHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MERRTKIVCTLGPAVASREGILGLVKAGMNVARMNMSHGDHADHEKNYQWVREATDETGR
CCCCEEEEEEECCHHHCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCC
AVGILADLQGPKIRLGRFIDGATVWENGEIVRITVDDIEGTHDRVSTTYKNLAKDAKPGD
EEEEEEECCCCCEEECEEECCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCC
RLLVDDGKVALRCVEVDGNDVVCEVVEGGPVSNNKGVSLPGMDISVPALSEKDIADLRFA
EEEEECCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHH
LNLGVDIIALSFVRSPADVELVHAIMDEEGRRCPVIAKLEKPEAMDALESIVLAFDGIMI
HHCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCEEE
ARGDLGVECPLEQVPLFQKRAIQIARENAKPVIVATQMLDSMIENLRPTRAEASDVANAV
EECCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHH
LDGADAVMLSGETSVGIDPANVVRTMSRIVSYAEIDGRVPNLAHIPRTKRGVISYSARDI
HCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHH
AERLNAKALVAFTSSGDTAKRVARLHSQLPLLVFTPHQEVRSQLALTWGVETFLTDEVKD
HHHCCCEEEEEEECCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCHHHHHCCCCCC
TDEMMRTIDEQLLALDTYSEDDMIVLVAGTPPGVQGNTNMIHVHHLGEDLNGL
HHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8074528; 12948626 [H]