Click here to switch to the map view.

The map label for this gene is rnhB

Identifier: 38234094

GI number: 38234094

Start: 1538491

End: 1539180

Strand: Reverse

Name: rnhB

Synonym: DIP1515

Alternate gene names: 38234094

Gene position: 1539180-1538491 (Counterclockwise)

Preceding gene: 38234095

Following gene: 38234093

Centisome position: 61.85

GC content: 49.86

Gene sequence:

>690_bases
GTGATCACTCAAGAACACTACTGTGCGTTGCCAGCTCCCAAAATTCGGACGCTGAAGCATCTTCGAACCTTCGAAGTTAC
TTTGGATAAACATGGTCTTGGGCCGGTAGCAGGTGTCGACGAAGCTGGACGTGGTTCATGCTGCGGACCTATCACGATTG
CGGCTTGTATTATGCCGCAGCGTCCCATCGCTCAGCTTCAGGCGCTCACAGACTCGAAAAAACTGAGTGCAGCAAAACGT
GCAGAACTATATCCGCTGATAAAAAAATACGCACTGGCGTGGTCGATCATTCATATCAGTGCCGCGGAAATTGATCGAGA
AGGCATACAGCATGCCAACATTTTCGGCATGCGACGAGCTATCGAAAAACTTGATGTGGCTCCTGGATATGTTTTAACGG
ATGCGATGAAAGTGCCGGGGCTACGGTGCCCCTCGCTTCCGATTATTGGAGGTGACGCAGCGGTGCGGTGCATTGCTGCG
GCAAGTGTTCTGGCAAAGCATTCGAGAGATCTCATCATGGATGATCTTGGTGGGAAATATCCCGAATATGGTTTGGAAGA
TCATAAAGGTTATGGGACGAAGAGTCACATGGATGCGGTGCGCCATCACGGCGCAACTCCTGAGCATCGATATAGTTATT
CCAATGTGAAGGCAGCACACGATCAATGGCTGCAAGAAAAGACACAATAA

Upstream 100 bases:

>100_bases
ATCAAGGCACCATCCCTAAAGAAAACCTCAAGGGGAAAGTTCAGTTCAAGATTTTCCCATTTAACCGTATTGGTGCAGTC
GAGGATTACGATATCCAACA

Downstream 100 bases:

>100_bases
GGGAGGTAAGCGATGAGCGCTGAAGAGCTCGATAATTATGAGGCGGAAGTTGAACTGTCCTTGTACCGCGAATACCGAGA
TGTGGTTAGCCAATTTTCGT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MITQEHYCALPAPKIRTLKHLRTFEVTLDKHGLGPVAGVDEAGRGSCCGPITIAACIMPQRPIAQLQALTDSKKLSAAKR
AELYPLIKKYALAWSIIHISAAEIDREGIQHANIFGMRRAIEKLDVAPGYVLTDAMKVPGLRCPSLPIIGGDAAVRCIAA
ASVLAKHSRDLIMDDLGGKYPEYGLEDHKGYGTKSHMDAVRHHGATPEHRYSYSNVKAAHDQWLQEKTQ

Sequences:

>Translated_229_residues
MITQEHYCALPAPKIRTLKHLRTFEVTLDKHGLGPVAGVDEAGRGSCCGPITIAACIMPQRPIAQLQALTDSKKLSAAKR
AELYPLIKKYALAWSIIHISAAEIDREGIQHANIFGMRRAIEKLDVAPGYVLTDAMKVPGLRCPSLPIIGGDAAVRCIAA
ASVLAKHSRDLIMDDLGGKYPEYGLEDHKGYGTKSHMDAVRHHGATPEHRYSYSNVKAAHDQWLQEKTQ
>Mature_229_residues
MITQEHYCALPAPKIRTLKHLRTFEVTLDKHGLGPVAGVDEAGRGSCCGPITIAACIMPQRPIAQLQALTDSKKLSAAKR
AELYPLIKKYALAWSIIHISAAEIDREGIQHANIFGMRRAIEKLDVAPGYVLTDAMKVPGLRCPSLPIIGGDAAVRCIAA
ASVLAKHSRDLIMDDLGGKYPEYGLEDHKGYGTKSHMDAVRHHGATPEHRYSYSNVKAAHDQWLQEKTQ

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=185, Percent_Identity=46.4864864864865, Blast_Score=153, Evalue=9e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_CORDI (Q6NGJ8)

Other databases:

- EMBL:   BX248358
- RefSeq:   NP_939861.1
- ProteinModelPortal:   Q6NGJ8
- SMR:   Q6NGJ8
- GeneID:   2650399
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1515
- NMPDR:   fig|257309.1.peg.1452
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- PhylomeDB:   Q6NGJ8
- ProtClustDB:   PRK00015
- BioCyc:   CDIP257309:DIP1515-MONOMER
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 25067; Mature: 25067

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITQEHYCALPAPKIRTLKHLRTFEVTLDKHGLGPVAGVDEAGRGSCCGPITIAACIMPQ
CCCCCCCCCCCCCHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
RPIAQLQALTDSKKLSAAKRAELYPLIKKYALAWSIIHISAAEIDREGIQHANIFGMRRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEKLDVAPGYVLTDAMKVPGLRCPSLPIIGGDAAVRCIAAASVLAKHSRDLIMDDLGGKY
HHHHCCCCCHHHHHHHHCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PEYGLEDHKGYGTKSHMDAVRHHGATPEHRYSYSNVKAAHDQWLQEKTQ
CCCCCCCCCCCCCHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MITQEHYCALPAPKIRTLKHLRTFEVTLDKHGLGPVAGVDEAGRGSCCGPITIAACIMPQ
CCCCCCCCCCCCCHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
RPIAQLQALTDSKKLSAAKRAELYPLIKKYALAWSIIHISAAEIDREGIQHANIFGMRRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEKLDVAPGYVLTDAMKVPGLRCPSLPIIGGDAAVRCIAAASVLAKHSRDLIMDDLGGKY
HHHHCCCCCHHHHHHHHCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PEYGLEDHKGYGTKSHMDAVRHHGATPEHRYSYSNVKAAHDQWLQEKTQ
CCCCCCCCCCCCCHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910