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The map label for this gene is ispG

Identifier: 38234077

GI number: 38234077

Start: 1522046

End: 1523221

Strand: Reverse

Name: ispG

Synonym: DIP1498

Alternate gene names: 38234077

Gene position: 1523221-1522046 (Counterclockwise)

Preceding gene: 38234078

Following gene: 38234076

Centisome position: 61.21

GC content: 50.6

Gene sequence:

>1176_bases
GTGTCCACCCCTACCGGTCAGCCCATCGGACTCGGATTGCCTGATGCACCGTTGCCCGTCCTCGCCCCACGCCGTAAGAC
CCGCCAATTAATGGTGGGAAATGTTGGTGTTGGCTCGGACTACCCAGTATCTGTTCAGTCGATGACAACCACCAAGACTC
ACGACGTCAACGCTACGTTGCAGCAAATTGCGCAGTTGACGGCCTCGGGATGTGACATTGTCCGTGTTGCCTGCCCCAAG
ACTGTTGATGCTGAGGCGTTGCCTGCTATTGCTAAAAAATCGCCTATTCCAGTGATTGCCGATATTCACTTCCAGCCTAA
ATATATATTTTCTGCTATTGATGCAGGTTGTGCTGCGGTTCGTGTTAACCCCGGCAATATCAAAGAATTTGATGGTCGTG
TTAAAGAAGTGGCAAAGGCAGCCGGAGACGCAGGAATCCCGATCCGCATTGGTGTCAACGCTGGATCCTTGGATAAGCGC
ATCATGGAAAAATACGGCAAGGCCACTCCGGAAGCATTGGTGGAATCAGCATTATGGGAAGCCGGTTTGTTTGAAGACTG
CGGTTTCGGCGATATTGCGATTTCTGTGAAGCACAATGATCCGGTCATCATGGTTGAGGCATACCGTCAGCTAGCAGCCC
AAAGCGACTATCCACTGCACCTAGGCGTTACTGAAGCTGGACCTGCATTCCAAGGAACGATTAAATCGTCAGTGGCTTTT
GGTTCATTGCTCTCGCAAGGTATTGGCGATACTATCCGTGTTTCTCTTTCTGCTGATCCAGTAGAAGAGATCAAAGTAGG
CGATCAGATTCTTCAGTCTCTAAACCTCCGTAAACGAGGGCTTGAAATCGTTTCTTGCCCATCGTGTGGTCGAGCACAGG
TCGATGTGTATTCGTTGGCTGAAGAGGTTACAGCCGCTTTGGACGGCATGAGTATTCCGTTGCGCGTAGCAGTAATGGGA
TGTGTGGTGAACGGTCCTGGCGAAGCACGAGATGCTGATTTGGGCGTTGCTTCCGGCAATGGCAAGGGACAAATCTTTGT
CCGTGGTGAAGTCATTAAGACAGTTCCAGAATCTCAAATTGTGGAGACTCTAATCGAAGAAGCCGTTCGATTGGCGGAAG
CAGAAGGCCTCGAAATTGAGGAGGGCGCTGGACCTCAGGTAAAAATTACTCGATAG

Upstream 100 bases:

>100_bases
AGGTGGCGGCGAAGGCTGCGCCACTACGGTGGTAGAATTTTCATCATCTGTCTTACACCTAGGTAACGATTGTTCATTTC
TGTATCAAGGAGCATGTTTA

Downstream 100 bases:

>100_bases
TAATTGACCTCTCGGAGGTTTCAAGGGTGCATCGGAATATTTCCGTGCACCCTTTCTTATTTGAGCTCGGGCGTTGCACA
ATAGAGTGACGTCACGCCAG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSTPTGQPIGLGLPDAPLPVLAPRRKTRQLMVGNVGVGSDYPVSVQSMTTTKTHDVNATLQQIAQLTASGCDIVRVACPK
TVDAEALPAIAKKSPIPVIADIHFQPKYIFSAIDAGCAAVRVNPGNIKEFDGRVKEVAKAAGDAGIPIRIGVNAGSLDKR
IMEKYGKATPEALVESALWEAGLFEDCGFGDIAISVKHNDPVIMVEAYRQLAAQSDYPLHLGVTEAGPAFQGTIKSSVAF
GSLLSQGIGDTIRVSLSADPVEEIKVGDQILQSLNLRKRGLEIVSCPSCGRAQVDVYSLAEEVTAALDGMSIPLRVAVMG
CVVNGPGEARDADLGVASGNGKGQIFVRGEVIKTVPESQIVETLIEEAVRLAEAEGLEIEEGAGPQVKITR

Sequences:

>Translated_391_residues
MSTPTGQPIGLGLPDAPLPVLAPRRKTRQLMVGNVGVGSDYPVSVQSMTTTKTHDVNATLQQIAQLTASGCDIVRVACPK
TVDAEALPAIAKKSPIPVIADIHFQPKYIFSAIDAGCAAVRVNPGNIKEFDGRVKEVAKAAGDAGIPIRIGVNAGSLDKR
IMEKYGKATPEALVESALWEAGLFEDCGFGDIAISVKHNDPVIMVEAYRQLAAQSDYPLHLGVTEAGPAFQGTIKSSVAF
GSLLSQGIGDTIRVSLSADPVEEIKVGDQILQSLNLRKRGLEIVSCPSCGRAQVDVYSLAEEVTAALDGMSIPLRVAVMG
CVVNGPGEARDADLGVASGNGKGQIFVRGEVIKTVPESQIVETLIEEAVRLAEAEGLEIEEGAGPQVKITR
>Mature_390_residues
STPTGQPIGLGLPDAPLPVLAPRRKTRQLMVGNVGVGSDYPVSVQSMTTTKTHDVNATLQQIAQLTASGCDIVRVACPKT
VDAEALPAIAKKSPIPVIADIHFQPKYIFSAIDAGCAAVRVNPGNIKEFDGRVKEVAKAAGDAGIPIRIGVNAGSLDKRI
MEKYGKATPEALVESALWEAGLFEDCGFGDIAISVKHNDPVIMVEAYRQLAAQSDYPLHLGVTEAGPAFQGTIKSSVAFG
SLLSQGIGDTIRVSLSADPVEEIKVGDQILQSLNLRKRGLEIVSCPSCGRAQVDVYSLAEEVTAALDGMSIPLRVAVMGC
VVNGPGEARDADLGVASGNGKGQIFVRGEVIKTVPESQIVETLIEEAVRLAEAEGLEIEEGAGPQVKITR

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=362, Percent_Identity=44.7513812154696, Blast_Score=310, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_CORDI (Q6NGL3)

Other databases:

- EMBL:   BX248358
- RefSeq:   NP_939844.1
- ProteinModelPortal:   Q6NGL3
- GeneID:   2650382
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1498
- NMPDR:   fig|257309.1.peg.1435
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- PhylomeDB:   Q6NGL3
- ProtClustDB:   PRK00366
- BioCyc:   CDIP257309:DIP1498-MONOMER
- BRENDA:   1.17.4.3
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 41011; Mature: 40879

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPTGQPIGLGLPDAPLPVLAPRRKTRQLMVGNVGVGSDYPVSVQSMTTTKTHDVNATL
CCCCCCCEEECCCCCCCCCCCCCCCHHCEEEEECCCCCCCCCEEEEEEEECCCCCHHHHH
QQIAQLTASGCDIVRVACPKTVDAEALPAIAKKSPIPVIADIHFQPKYIFSAIDAGCAAV
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCEEE
RVNPGNIKEFDGRVKEVAKAAGDAGIPIRIGVNAGSLDKRIMEKYGKATPEALVESALWE
EECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHH
AGLFEDCGFGDIAISVKHNDPVIMVEAYRQLAAQSDYPLHLGVTEAGPAFQGTIKSSVAF
CCCCCCCCCCEEEEEEECCCCEEEHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHH
GSLLSQGIGDTIRVSLSADPVEEIKVGDQILQSLNLRKRGLEIVSCPSCGRAQVDVYSLA
HHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHH
EEVTAALDGMSIPLRVAVMGCVVNGPGEARDADLGVASGNGKGQIFVRGEVIKTVPESQI
HHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCEEECCCCCEEEEEECHHHHHCCHHHH
VETLIEEAVRLAEAEGLEIEEGAGPQVKITR
HHHHHHHHHHHHHCCCCEEECCCCCEEEEEC
>Mature Secondary Structure 
STPTGQPIGLGLPDAPLPVLAPRRKTRQLMVGNVGVGSDYPVSVQSMTTTKTHDVNATL
CCCCCCEEECCCCCCCCCCCCCCCHHCEEEEECCCCCCCCCEEEEEEEECCCCCHHHHH
QQIAQLTASGCDIVRVACPKTVDAEALPAIAKKSPIPVIADIHFQPKYIFSAIDAGCAAV
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCEEE
RVNPGNIKEFDGRVKEVAKAAGDAGIPIRIGVNAGSLDKRIMEKYGKATPEALVESALWE
EECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHH
AGLFEDCGFGDIAISVKHNDPVIMVEAYRQLAAQSDYPLHLGVTEAGPAFQGTIKSSVAF
CCCCCCCCCCEEEEEEECCCCEEEHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHH
GSLLSQGIGDTIRVSLSADPVEEIKVGDQILQSLNLRKRGLEIVSCPSCGRAQVDVYSLA
HHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHH
EEVTAALDGMSIPLRVAVMGCVVNGPGEARDADLGVASGNGKGQIFVRGEVIKTVPESQI
HHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCEEECCCCCEEEEEECHHHHHCCHHHH
VETLIEEAVRLAEAEGLEIEEGAGPQVKITR
HHHHHHHHHHHHHCCCCEEECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910