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The map label for this gene is truB

Identifier: 38234052

GI number: 38234052

Start: 1491198

End: 1492100

Strand: Direct

Name: truB

Synonym: DIP1471

Alternate gene names: 38234052

Gene position: 1491198-1492100 (Clockwise)

Preceding gene: 38234040

Following gene: 38234062

Centisome position: 59.92

GC content: 50.61

Gene sequence:

>903_bases
ATGAATGATGCCCTCGCAGACTCAGGCCTTGTGATTGTCGACAAACCCCAAGGAATGACCTCCCACGACGTTGTTTCTAA
AATCCGTCGGACCTTTTCCACCAAAAAGGTGGGTCATGCCGGAACCCTCGACCCTATGGCTACAGGAGTGCTCGTATTAG
GACTAGAGCGCGGGACCAAATTCCTAGCACATATGGTTGCGTCTACAAAGTCCTATACGGCCACTATTCGTCTCGGTGCT
GCTACTACTACCGACGACCGTGAAGGCGAAACGATCACTTCAGCTTCACCAGATCAACTGGCAGGAATCACCGAAACCAA
GATCTCCGATGCCGTTAAGCAGTTTCGGGGATCTATCATGCAACGTCCTGCCGCAGTGAGCGCTATCAAGATAGATGGGA
AAAGAGCGCATCAACGCGTACGAGAAGGTGAAAAAGTAGAAATTCCAGCACGGCCGGTCACCATTTCTCGGTATGACATT
TTAGAGATTCGTCGAGACGCAGCTTTCATCGACATCGACGTAGAAGTTGATTGTTCTTCCGGAACCTACATTCGGTCACT
AGCCCGTGATCTCGGCGAAGAACTCGGAGTAGGTGGGCACTTAACCGCGTTACGACGAACACAGGTAGGACCGTTTACGC
TCGATAATGCTGTAACACTCGAAAAATTGGAAGAAAACCCACACGTATCTTTGACCCTTGACCAGGCATTAGCAGCGAGC
TACCCAGTTCTTTCTGTTTCAGAAAAAGAGGCGAGTGATCTCGCCATGGGCAAATGGCTCACTCCACGCGGTCTGAAAGG
AATCCATGCAGCGGTCGATCCTCATGGACGTGCTATCGCCTTGGTAAAAGAACAAGGCAAACGGCTAGCCACCATATTTG
TGGCACGTCCATCGACCCTTTAA

Upstream 100 bases:

>100_bases
TACGCCAATGGTGATAACTGAGGCTTTCAAGTCTGCTGGAATATCTTCTAATCTGTGCCAAATGTCCACCTGATCCACTT
TAAGCCATAGACTGTCATCT

Downstream 100 bases:

>100_bases
CCCATCGCGGGGATGACAGTGGTTGCTACGACATAGCCATCATGTATTGCCCACTTTCCTTCGATAAAAGGAAACGGGGT
GGGCCGAATGAGCAAATAAG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MNDALADSGLVIVDKPQGMTSHDVVSKIRRTFSTKKVGHAGTLDPMATGVLVLGLERGTKFLAHMVASTKSYTATIRLGA
ATTTDDREGETITSASPDQLAGITETKISDAVKQFRGSIMQRPAAVSAIKIDGKRAHQRVREGEKVEIPARPVTISRYDI
LEIRRDAAFIDIDVEVDCSSGTYIRSLARDLGEELGVGGHLTALRRTQVGPFTLDNAVTLEKLEENPHVSLTLDQALAAS
YPVLSVSEKEASDLAMGKWLTPRGLKGIHAAVDPHGRAIALVKEQGKRLATIFVARPSTL

Sequences:

>Translated_300_residues
MNDALADSGLVIVDKPQGMTSHDVVSKIRRTFSTKKVGHAGTLDPMATGVLVLGLERGTKFLAHMVASTKSYTATIRLGA
ATTTDDREGETITSASPDQLAGITETKISDAVKQFRGSIMQRPAAVSAIKIDGKRAHQRVREGEKVEIPARPVTISRYDI
LEIRRDAAFIDIDVEVDCSSGTYIRSLARDLGEELGVGGHLTALRRTQVGPFTLDNAVTLEKLEENPHVSLTLDQALAAS
YPVLSVSEKEASDLAMGKWLTPRGLKGIHAAVDPHGRAIALVKEQGKRLATIFVARPSTL
>Mature_300_residues
MNDALADSGLVIVDKPQGMTSHDVVSKIRRTFSTKKVGHAGTLDPMATGVLVLGLERGTKFLAHMVASTKSYTATIRLGA
ATTTDDREGETITSASPDQLAGITETKISDAVKQFRGSIMQRPAAVSAIKIDGKRAHQRVREGEKVEIPARPVTISRYDI
LEIRRDAAFIDIDVEVDCSSGTYIRSLARDLGEELGVGGHLTALRRTQVGPFTLDNAVTLEKLEENPHVSLTLDQALAAS
YPVLSVSEKEASDLAMGKWLTPRGLKGIHAAVDPHGRAIALVKEQGKRLATIFVARPSTL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=229, Percent_Identity=39.7379912663755, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI215599015, Length=217, Percent_Identity=33.1797235023041, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4503337, Length=217, Percent_Identity=33.1797235023041, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI2367200, Length=222, Percent_Identity=39.6396396396396, Blast_Score=168, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17553978, Length=207, Percent_Identity=29.951690821256, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323204, Length=216, Percent_Identity=31.0185185185185, Blast_Score=102, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6324037, Length=132, Percent_Identity=37.8787878787879, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364189, Length=204, Percent_Identity=29.9019607843137, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI281364187, Length=204, Percent_Identity=29.9019607843137, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI281364185, Length=204, Percent_Identity=29.9019607843137, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI281364183, Length=204, Percent_Identity=29.9019607843137, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI62471759, Length=204, Percent_Identity=29.9019607843137, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI17975520, Length=204, Percent_Identity=29.9019607843137, Blast_Score=97, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_CORDI (P60343)

Other databases:

- EMBL:   BX248358
- RefSeq:   NP_939819.1
- ProteinModelPortal:   P60343
- SMR:   P60343
- GeneID:   2650356
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1471
- NMPDR:   fig|257309.1.peg.1410
- HOGENOM:   HBG397258
- OMA:   YPAHMSK
- PhylomeDB:   P60343
- ProtClustDB:   PRK03287
- BioCyc:   CDIP257309:DIP1471-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR015225
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09142 TruB_C; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 32242; Mature: 32242

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: ACT_SITE 44-44

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDALADSGLVIVDKPQGMTSHDVVSKIRRTFSTKKVGHAGTLDPMATGVLVLGLERGTK
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCEEEEEEHHHHH
FLAHMVASTKSYTATIRLGAATTTDDREGETITSASPDQLAGITETKISDAVKQFRGSIM
HHHHHHHCCCCEEEEEEECCEECCCCCCCCEECCCCCHHHCCCHHHHHHHHHHHHHHHHH
QRPAAVSAIKIDGKRAHQRVREGEKVEIPARPVTISRYDILEIRRDAAFIDIDVEVDCSS
CCCCCEEEEEECCHHHHHHHHCCCEEECCCCCEEECCHHHHHHHCCCEEEEEEEEEECCC
GTYIRSLARDLGEELGVGGHLTALRRTQVGPFTLDNAVTLEKLEENPHVSLTLDQALAAS
CHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEECCCCEEHHHHCCCCCEEEEEHHHHHCC
YPVLSVSEKEASDLAMGKWLTPRGLKGIHAAVDPHGRAIALVKEQGKRLATIFVARPSTL
CCEEEECCCCHHHHHHCCCCCCCCCCCCEEEECCCCCEEEEEECCCCEEEEEEEECCCCC
>Mature Secondary Structure
MNDALADSGLVIVDKPQGMTSHDVVSKIRRTFSTKKVGHAGTLDPMATGVLVLGLERGTK
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCEEEEEEHHHHH
FLAHMVASTKSYTATIRLGAATTTDDREGETITSASPDQLAGITETKISDAVKQFRGSIM
HHHHHHHCCCCEEEEEEECCEECCCCCCCCEECCCCCHHHCCCHHHHHHHHHHHHHHHHH
QRPAAVSAIKIDGKRAHQRVREGEKVEIPARPVTISRYDILEIRRDAAFIDIDVEVDCSS
CCCCCEEEEEECCHHHHHHHHCCCEEECCCCCEEECCHHHHHHHCCCEEEEEEEEEECCC
GTYIRSLARDLGEELGVGGHLTALRRTQVGPFTLDNAVTLEKLEENPHVSLTLDQALAAS
CHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEECCCCEEHHHHCCCCCEEEEEHHHHHCC
YPVLSVSEKEASDLAMGKWLTPRGLKGIHAAVDPHGRAIALVKEQGKRLATIFVARPSTL
CCEEEECCCCHHHHHHCCCCCCCCCCCCEEEECCCCCEEEEEECCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910