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The map label for this gene is gpsI

Identifier: 38234048

GI number: 38234048

Start: 1486307

End: 1488574

Strand: Reverse

Name: gpsI

Synonym: DIP1467

Alternate gene names: 38234048

Gene position: 1488574-1486307 (Counterclockwise)

Preceding gene: 38234049

Following gene: 38234047

Centisome position: 59.81

GC content: 50.75

Gene sequence:

>2268_bases
TTGAGCACAAAGCCTTCCAACGTCGTCTTTAACATTGACGAGGAATTTGGAATCACCGAGGCAATCGCAACCATCGATAA
CGGTGATTTTGGAAAGCGCACCATCCGTTTTGAGACCGGCCAGTTGGCACGCCAAGCAGACGGATCAGTTACAACCTATC
TTGACGACGACACGATGTTGTTGGCAACGACCACGGCATCTAATCAGCCACGTGAAGGATTCGATTTCTTCCCGCTGACA
GTCGACGTTGAAGAGCGTATGTACGCAGCTGGTAGGATCCCAGGATCGTTCTTCCGTCGTGAAGGCCGTCCTTCCACCGA
AGCAATCTTGGCTTGCCGTTTGATCGACCGCCCGTTGCGCCCCACCTTTGTTAAGGGGCTACGCAACGAAGTTCAGGTAG
TTATCACTGTTCTTTCCGTAAACCCGCAAGACATGTACGATGTCGTGGCAATTAACGGTGCCTCTGCAGCAACTCAGTTG
TCAGGTTTGCCGGTTTCTGGTCCTGTTGGCGGTGTCCGAATGGCACTGATCGCAGACGATGCTCACCCTAAGGGGCAGTG
GATTGCTTTCCCTACTCATGAACAGCATGAACAAGCACTCTTTGAGCTCGTCGTAGCAGGACGTATTGTCTCGAAGAAGC
AAGGTAAAAAGACCGTTGATGATGTTGCCATCATGATGGTTGAGGCTGGTGCTACTGAGCAGGTTGTAGATCGAGTTAAA
GCTGGAGCTCCAGCTCCTACAGAATCTGTGGTTGCAGAAGGTCTTGAGGCTGCTAAACCATTCATTGAGGTTCTTTGCCG
TGCGCAGGCTGGATTGGCCGAGCGTGCAGCCAAGGAAACCCAAGAATTCCCACTCTTCCCGCCATACACTGACGTTGTTT
ACGATGCAGTGGAGAAGAAGGTTTCTAAGAAACTGCGTTCGTTGCTCACGATCAAGTCCAAGCAAGATCGTGATGAGGCA
ACGAATGCTTACATGGAAGAAATCGAAGCAGATCTGATTGCCCAGCTCGGATCTGATGATGAGGCGGCTGCATCTAAGGC
AATTCGTGCAGCATACAATGCTGTAATGAAGAAGATTGTCCGTCATATGATCTTGACGGAACACTTCCGCATCGACGGTC
GCGGTGTCACCGATATTCGTGATTTAGGTGTGGAAGTTGACCTCATCCCACGCGCTCATGGTTCGTCCTTGTTCGAGCGT
GGCGAGACTCAGATTCTGGGTGTGACCACTCTCGATATGCTCAAGATGGAGCAGCAGATCGATTCATTGACTCCAGCAAC
TTCGAAGCGTTATATCCACCACTACAACTTCCCACCGTATTCCACTGGTGAAACTGGCCGTGTTGGCTCACCTAAGCGTC
GCGAAATTGGCCACGGTGCTTTGGCTGAGCGTGCACTGGTTCCTGTGATCCCAAGTCGTGAAGAGTTCCCTTATGCAATC
CGACAGGTGTCTGAGGCTTTGGGCTCGAACGGTTCTACCTCGATGGGCTCTGTATGTGCGTCCACCTTGTCTTTGTACAA
CGCAGGTGTACCGTTGAAAGCTCCCGTTGCTGGTATTGCGATGGGCTTGGTGTCTGATGAAGTAGACGGTGAGACTCGTT
ATGTCGCTTTGACTGACATCTTGGGTGCAGAAGACGCCTTTGGTGATATGGACTTCAAGGTTGCTGGTACTCGCCAGTTC
ATTACCGCTTTGCAGCTAGATACAAAGCTTGATGGCATTCCTTCTGAGGTGCTTGCTCAGGCGCTATCCCAGGCGAATGA
TGCGCGTAACACCATTTTGGATACGATGGCAGAAGTAATCGAAACTCCAGATGAGATGAGCGACTACGCGCCACGTATTA
CTGCGATTACCGTTCCAGTGAACAAGATCGGTGAGGTTATCGGTCCAAAGGGCAAGACCATTAACTCTATTACCGAAGAA
ACTGGCGCTAACATTTCTATTGAAGAAGACGGCACAGTTTATGTTTCGGCCGCCAGCGGTGCTGCTGCAGAGGCTGCAAT
CGAAAAGATTAACGCTATCGCTAACCCACAGCTGCCTAAGGTCGGAGAGCGTTTCCTCGGCACTGTCGTAAAGACCACTG
CATTTGGTGCTTTCGTATCCTTGCTGCCAGGACGCGATGGACTGGTTCACATCTCCAAGCTTGGTGGTGGCAAGCGTATC
GAAAAGGTTGAAGATGTAGTCAACGTGGGAGATAAACTCGAGGTTGAAATCCTAGATATTGACAACCGTGGCAAGATTTC
CTTGGCTCCAGTGAAAAACGAGGACTAA

Upstream 100 bases:

>100_bases
TCATGCTAGAGTTTGATCGTTGCCATAAGAAGAATGAATTTTCGGCAAGCAAGCGACACCGATGATCGCATGCACGAAAA
ATTAAGAGGAGAAGCCCACT

Downstream 100 bases:

>100_bases
ATTCTCGGTGATCTAAACCATACTCAAGCCCCTTGCCTCAGCTTCTAGAGGGATTCCCTAGGAAAGCTGGGCAAGGGGCT
TGAGTATTTCTGTGTCGTTG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 755; Mature: 754

Protein sequence:

>755_residues
MSTKPSNVVFNIDEEFGITEAIATIDNGDFGKRTIRFETGQLARQADGSVTTYLDDDTMLLATTTASNQPREGFDFFPLT
VDVEERMYAAGRIPGSFFRREGRPSTEAILACRLIDRPLRPTFVKGLRNEVQVVITVLSVNPQDMYDVVAINGASAATQL
SGLPVSGPVGGVRMALIADDAHPKGQWIAFPTHEQHEQALFELVVAGRIVSKKQGKKTVDDVAIMMVEAGATEQVVDRVK
AGAPAPTESVVAEGLEAAKPFIEVLCRAQAGLAERAAKETQEFPLFPPYTDVVYDAVEKKVSKKLRSLLTIKSKQDRDEA
TNAYMEEIEADLIAQLGSDDEAAASKAIRAAYNAVMKKIVRHMILTEHFRIDGRGVTDIRDLGVEVDLIPRAHGSSLFER
GETQILGVTTLDMLKMEQQIDSLTPATSKRYIHHYNFPPYSTGETGRVGSPKRREIGHGALAERALVPVIPSREEFPYAI
RQVSEALGSNGSTSMGSVCASTLSLYNAGVPLKAPVAGIAMGLVSDEVDGETRYVALTDILGAEDAFGDMDFKVAGTRQF
ITALQLDTKLDGIPSEVLAQALSQANDARNTILDTMAEVIETPDEMSDYAPRITAITVPVNKIGEVIGPKGKTINSITEE
TGANISIEEDGTVYVSAASGAAAEAAIEKINAIANPQLPKVGERFLGTVVKTTAFGAFVSLLPGRDGLVHISKLGGGKRI
EKVEDVVNVGDKLEVEILDIDNRGKISLAPVKNED

Sequences:

>Translated_755_residues
MSTKPSNVVFNIDEEFGITEAIATIDNGDFGKRTIRFETGQLARQADGSVTTYLDDDTMLLATTTASNQPREGFDFFPLT
VDVEERMYAAGRIPGSFFRREGRPSTEAILACRLIDRPLRPTFVKGLRNEVQVVITVLSVNPQDMYDVVAINGASAATQL
SGLPVSGPVGGVRMALIADDAHPKGQWIAFPTHEQHEQALFELVVAGRIVSKKQGKKTVDDVAIMMVEAGATEQVVDRVK
AGAPAPTESVVAEGLEAAKPFIEVLCRAQAGLAERAAKETQEFPLFPPYTDVVYDAVEKKVSKKLRSLLTIKSKQDRDEA
TNAYMEEIEADLIAQLGSDDEAAASKAIRAAYNAVMKKIVRHMILTEHFRIDGRGVTDIRDLGVEVDLIPRAHGSSLFER
GETQILGVTTLDMLKMEQQIDSLTPATSKRYIHHYNFPPYSTGETGRVGSPKRREIGHGALAERALVPVIPSREEFPYAI
RQVSEALGSNGSTSMGSVCASTLSLYNAGVPLKAPVAGIAMGLVSDEVDGETRYVALTDILGAEDAFGDMDFKVAGTRQF
ITALQLDTKLDGIPSEVLAQALSQANDARNTILDTMAEVIETPDEMSDYAPRITAITVPVNKIGEVIGPKGKTINSITEE
TGANISIEEDGTVYVSAASGAAAEAAIEKINAIANPQLPKVGERFLGTVVKTTAFGAFVSLLPGRDGLVHISKLGGGKRI
EKVEDVVNVGDKLEVEILDIDNRGKISLAPVKNED
>Mature_754_residues
STKPSNVVFNIDEEFGITEAIATIDNGDFGKRTIRFETGQLARQADGSVTTYLDDDTMLLATTTASNQPREGFDFFPLTV
DVEERMYAAGRIPGSFFRREGRPSTEAILACRLIDRPLRPTFVKGLRNEVQVVITVLSVNPQDMYDVVAINGASAATQLS
GLPVSGPVGGVRMALIADDAHPKGQWIAFPTHEQHEQALFELVVAGRIVSKKQGKKTVDDVAIMMVEAGATEQVVDRVKA
GAPAPTESVVAEGLEAAKPFIEVLCRAQAGLAERAAKETQEFPLFPPYTDVVYDAVEKKVSKKLRSLLTIKSKQDRDEAT
NAYMEEIEADLIAQLGSDDEAAASKAIRAAYNAVMKKIVRHMILTEHFRIDGRGVTDIRDLGVEVDLIPRAHGSSLFERG
ETQILGVTTLDMLKMEQQIDSLTPATSKRYIHHYNFPPYSTGETGRVGSPKRREIGHGALAERALVPVIPSREEFPYAIR
QVSEALGSNGSTSMGSVCASTLSLYNAGVPLKAPVAGIAMGLVSDEVDGETRYVALTDILGAEDAFGDMDFKVAGTRQFI
TALQLDTKLDGIPSEVLAQALSQANDARNTILDTMAEVIETPDEMSDYAPRITAITVPVNKIGEVIGPKGKTINSITEET
GANISIEEDGTVYVSAASGAAAEAAIEKINAIANPQLPKVGERFLGTVVKTTAFGAFVSLLPGRDGLVHISKLGGGKRIE
KVEDVVNVGDKLEVEILDIDNRGKISLAPVKNED

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=727, Percent_Identity=35.7634112792297, Blast_Score=388, Evalue=1e-108,
Organism=Escherichia coli, GI145693187, Length=725, Percent_Identity=43.7241379310345, Blast_Score=537, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI115534063, Length=737, Percent_Identity=32.0217096336499, Blast_Score=304, Evalue=1e-82,
Organism=Drosophila melanogaster, GI281362905, Length=725, Percent_Identity=34.4827586206897, Blast_Score=384, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24651641, Length=725, Percent_Identity=34.4827586206897, Blast_Score=384, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24651643, Length=725, Percent_Identity=34.4827586206897, Blast_Score=384, Evalue=1e-106,
Organism=Drosophila melanogaster, GI161079377, Length=670, Percent_Identity=34.3283582089552, Blast_Score=353, Evalue=2e-97,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_CORDI (Q6NGP1)

Other databases:

- EMBL:   BX248358
- RefSeq:   NP_939815.1
- HSSP:   P05055
- ProteinModelPortal:   Q6NGP1
- SMR:   Q6NGP1
- GeneID:   2650352
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1467
- NMPDR:   fig|257309.1.peg.1406
- HOGENOM:   HBG382411
- OMA:   KRVDNVD
- ProtClustDB:   PRK11824
- BioCyc:   CDIP257309:DIP1467-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR014069
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591
- TIGRFAMs:   TIGR02696

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 81267; Mature: 81136

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKPSNVVFNIDEEFGITEAIATIDNGDFGKRTIRFETGQLARQADGSVTTYLDDDTML
CCCCCCCEEEEECHHCCHHHHHHCCCCCCCCCEEEEEEHHHHHHHCCCCEEEEECCCEEE
LATTTASNQPREGFDFFPLTVDVEERMYAAGRIPGSFFRREGRPSTEAILACRLIDRPLR
EEEECCCCCCCCCCCEEEEEEEHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHCCCCC
PTFVKGLRNEVQVVITVLSVNPQDMYDVVAINGASAATQLSGLPVSGPVGGVRMALIADD
HHHHHHHHHCEEEEEEEEECCCCCCEEEEEECCCCHHHHCCCCCCCCCCCCEEEEEEECC
AHPKGQWIAFPTHEQHEQALFELVVAGRIVSKKQGKKTVDDVAIMMVEAGATEQVVDRVK
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEEECCCHHHHHHHHH
AGAPAPTESVVAEGLEAAKPFIEVLCRAQAGLAERAAKETQEFPLFPPYTDVVYDAVEKK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VSKKLRSLLTIKSKQDRDEATNAYMEEIEADLIAQLGSDDEAAASKAIRAAYNAVMKKIV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RHMILTEHFRIDGRGVTDIRDLGVEVDLIPRAHGSSLFERGETQILGVTTLDMLKMEQQI
HHHHHHHHEEECCCCCCHHHHCCCEEEEECCCCCCHHHHCCCCEEEEEHHHHHHHHHHHH
DSLTPATSKRYIHHYNFPPYSTGETGRVGSPKRREIGHGALAERALVPVIPSREEFPYAI
HHCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHCCCCHHHHCCCCCCCCCCCCCCHHH
RQVSEALGSNGSTSMGSVCASTLSLYNAGVPLKAPVAGIAMGLVSDEVDGETRYVALTDI
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEH
LGAEDAFGDMDFKVAGTRQFITALQLDTKLDGIPSEVLAQALSQANDARNTILDTMAEVI
HCCCCCCCCCCEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ETPDEMSDYAPRITAITVPVNKIGEVIGPKGKTINSITEETGANISIEEDGTVYVSAASG
CCCHHHHHHCCCEEEEEECHHHHHHHHCCCCCCHHHHHHHHCCEEEEECCCEEEEEECCC
AAAEAAIEKINAIANPQLPKVGERFLGTVVKTTAFGAFVSLLPGRDGLVHISKLGGGKRI
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHH
EKVEDVVNVGDKLEVEILDIDNRGKISLAPVKNED
HHHHHHHCCCCEEEEEEEECCCCCCEEEECCCCCC
>Mature Secondary Structure 
STKPSNVVFNIDEEFGITEAIATIDNGDFGKRTIRFETGQLARQADGSVTTYLDDDTML
CCCCCCEEEEECHHCCHHHHHHCCCCCCCCCEEEEEEHHHHHHHCCCCEEEEECCCEEE
LATTTASNQPREGFDFFPLTVDVEERMYAAGRIPGSFFRREGRPSTEAILACRLIDRPLR
EEEECCCCCCCCCCCEEEEEEEHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHCCCCC
PTFVKGLRNEVQVVITVLSVNPQDMYDVVAINGASAATQLSGLPVSGPVGGVRMALIADD
HHHHHHHHHCEEEEEEEEECCCCCCEEEEEECCCCHHHHCCCCCCCCCCCCEEEEEEECC
AHPKGQWIAFPTHEQHEQALFELVVAGRIVSKKQGKKTVDDVAIMMVEAGATEQVVDRVK
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEEECCCHHHHHHHHH
AGAPAPTESVVAEGLEAAKPFIEVLCRAQAGLAERAAKETQEFPLFPPYTDVVYDAVEKK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VSKKLRSLLTIKSKQDRDEATNAYMEEIEADLIAQLGSDDEAAASKAIRAAYNAVMKKIV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RHMILTEHFRIDGRGVTDIRDLGVEVDLIPRAHGSSLFERGETQILGVTTLDMLKMEQQI
HHHHHHHHEEECCCCCCHHHHCCCEEEEECCCCCCHHHHCCCCEEEEEHHHHHHHHHHHH
DSLTPATSKRYIHHYNFPPYSTGETGRVGSPKRREIGHGALAERALVPVIPSREEFPYAI
HHCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHCCCCHHHHCCCCCCCCCCCCCCHHH
RQVSEALGSNGSTSMGSVCASTLSLYNAGVPLKAPVAGIAMGLVSDEVDGETRYVALTDI
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEH
LGAEDAFGDMDFKVAGTRQFITALQLDTKLDGIPSEVLAQALSQANDARNTILDTMAEVI
HCCCCCCCCCCEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ETPDEMSDYAPRITAITVPVNKIGEVIGPKGKTINSITEETGANISIEEDGTVYVSAASG
CCCHHHHHHCCCEEEEEECHHHHHHHHCCCCCCHHHHHHHHCCEEEEECCCEEEEEECCC
AAAEAAIEKINAIANPQLPKVGERFLGTVVKTTAFGAFVSLLPGRDGLVHISKLGGGKRI
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHH
EKVEDVVNVGDKLEVEILDIDNRGKISLAPVKNED
HHHHHHHCCCCEEEEEEEECCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910