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The map label for this gene is miaB

Identifier: 38234029

GI number: 38234029

Start: 1467450

End: 1469000

Strand: Reverse

Name: miaB

Synonym: DIP1448

Alternate gene names: 38234029

Gene position: 1469000-1467450 (Counterclockwise)

Preceding gene: 38234030

Following gene: 38234028

Centisome position: 59.03

GC content: 48.94

Gene sequence:

>1551_bases
ATGTTTAACGATATAGAGTCTCACCGCGTACCTAATGTAGGTCAGGGAAGATCCTTTGAAGTAAGAACGTTCGGTTGCCA
AATGAACGTTCATGATTCGGAGCGTCTATCTGGTCTGTTAGAAGAAGCCGGATATCATGCGGTGGCTGATGGTGAAGAGC
CAGATCTTGTCGTGTTTAACACCTGTGCGGTGCGAGAAAACGCAGATAAGCGACTGTATGGCACCCTAGGACAGTTGCGG
TCGGCGAAGGAAAAGAACCCTCGCATGCAAATTGCCGTTGGCGGATGTTTGGCCCAAAAAGACAAAGACACGGTGGTTGC
TAAAGCTCCTTGGGTCGATGCGGTTTTTGGTACTCACAATATGGGAGCATTGCCATCGCTTTTGAGCCGATCCGAGCACA
ACAAACGCGCCGAGGTGGAAATTGTTGACTCGCTCGAACAATTTCCATCAGTACTACCTGCAAAACGTGAGTCAGCATAT
GCAGGTTGGGTATCGGTTTCGGTCGGATGTAACAACACCTGTACTTTCTGTATCGTGCCATCGCTTCGTGGCAAAGAAGT
CGACCGTCGACCAGGTGACATTTTGGCGGAAGTACAAGCCCTCGTTGATCAAGGAGTCAGTGAAGTTACCCTTCTTGGCC
AAAACGTCAATGCTTATGGTGTCAACTTCTCTGATCCTGATATCCAGCGCGATCGCTTTGCATTCTCTAAACTCTTGCGC
GCGTGCGGAAAGATTGAAGGTCTCGAACGTCTACGTTTCACAAGTCCTCATCCCGCAGAATTCACGCATGATGTGATTGA
CGCTATGGCGGAAACCCCCAATGTGTGCCCACAGCTCCACATGCCGCTCCAGTCTGGTTCCGACAAAGTCTTGAAGGAAA
TGCGTCGGTCTTATCGTTCAAAGAAATTTTTGGGAATTCTCGAGGAGGTGCGAGCAAAGATTCCGCATGCTTCTATTACC
ACTGACATCATCGTAGGATTCCCCGGAGAAACCGAAGAGGATTTCCAAGAGACGCTGAATGTAGTTGAAAAAGCACGTTT
TACTAGCGCTTATACTTTCCAATACAGTCCTCGCCCTGGTACTCCAGCAGCAGACTATGCTGATCAGGTTCCTAAAGAAG
TAGTCCAAGACCGATATGAACGACTCCTTGCGCTGCAAGAACGCATTAGTACTGAAGAAAATGCAAAGCTAATCGGTACT
GAGGTTGAACTGCTTGTCCAAGCATCTGGTGGACGAAAAAATGATAAAACTCAGCGTATGACTGGGCGTTCTCGTGATGG
CCGTCTAGTGCATTTCGATCCACAAGGACATGTTGATGGAGATATTCGCCCAGGAGATGTGATCACCACAGTAGTAACTG
AGGCAAAGCCATTCTTCCTGATTGCAGACTCTGGTGTTTTACAGCATCGCCGTACAAAAGCAGGCGATATGAGTGCAGCT
GGTAAAGTTCCCACCACTGCTCCGGTAGGCGTTGGGTTGGGTCTACCGTCCATTGGCTCTCCGGCACAAAAGCGTTCAGA
AACGTCGAAAAGCAGTGGATGTGGCTGTTAG

Upstream 100 bases:

>100_bases
GCTTGTACAATGTCTGCTCGTGTCATCTGCAACCGAACAAAACACTAATTCAGTCATTAACGATTCTGGTTCTTTCTCTG
AGCGTACTGCGAAAGAAGAA

Downstream 100 bases:

>100_bases
CTGTAATGTGGCAGTAACCTAGAGCGTTATTAATTCTAAGGAGAAGAACATGAGCGAATCGTCTGGATCTGCTACCGAAC
AGGAATTAAGAATCAAAGAG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MFNDIESHRVPNVGQGRSFEVRTFGCQMNVHDSERLSGLLEEAGYHAVADGEEPDLVVFNTCAVRENADKRLYGTLGQLR
SAKEKNPRMQIAVGGCLAQKDKDTVVAKAPWVDAVFGTHNMGALPSLLSRSEHNKRAEVEIVDSLEQFPSVLPAKRESAY
AGWVSVSVGCNNTCTFCIVPSLRGKEVDRRPGDILAEVQALVDQGVSEVTLLGQNVNAYGVNFSDPDIQRDRFAFSKLLR
ACGKIEGLERLRFTSPHPAEFTHDVIDAMAETPNVCPQLHMPLQSGSDKVLKEMRRSYRSKKFLGILEEVRAKIPHASIT
TDIIVGFPGETEEDFQETLNVVEKARFTSAYTFQYSPRPGTPAADYADQVPKEVVQDRYERLLALQERISTEENAKLIGT
EVELLVQASGGRKNDKTQRMTGRSRDGRLVHFDPQGHVDGDIRPGDVITTVVTEAKPFFLIADSGVLQHRRTKAGDMSAA
GKVPTTAPVGVGLGLPSIGSPAQKRSETSKSSGCGC

Sequences:

>Translated_516_residues
MFNDIESHRVPNVGQGRSFEVRTFGCQMNVHDSERLSGLLEEAGYHAVADGEEPDLVVFNTCAVRENADKRLYGTLGQLR
SAKEKNPRMQIAVGGCLAQKDKDTVVAKAPWVDAVFGTHNMGALPSLLSRSEHNKRAEVEIVDSLEQFPSVLPAKRESAY
AGWVSVSVGCNNTCTFCIVPSLRGKEVDRRPGDILAEVQALVDQGVSEVTLLGQNVNAYGVNFSDPDIQRDRFAFSKLLR
ACGKIEGLERLRFTSPHPAEFTHDVIDAMAETPNVCPQLHMPLQSGSDKVLKEMRRSYRSKKFLGILEEVRAKIPHASIT
TDIIVGFPGETEEDFQETLNVVEKARFTSAYTFQYSPRPGTPAADYADQVPKEVVQDRYERLLALQERISTEENAKLIGT
EVELLVQASGGRKNDKTQRMTGRSRDGRLVHFDPQGHVDGDIRPGDVITTVVTEAKPFFLIADSGVLQHRRTKAGDMSAA
GKVPTTAPVGVGLGLPSIGSPAQKRSETSKSSGCGC
>Mature_516_residues
MFNDIESHRVPNVGQGRSFEVRTFGCQMNVHDSERLSGLLEEAGYHAVADGEEPDLVVFNTCAVRENADKRLYGTLGQLR
SAKEKNPRMQIAVGGCLAQKDKDTVVAKAPWVDAVFGTHNMGALPSLLSRSEHNKRAEVEIVDSLEQFPSVLPAKRESAY
AGWVSVSVGCNNTCTFCIVPSLRGKEVDRRPGDILAEVQALVDQGVSEVTLLGQNVNAYGVNFSDPDIQRDRFAFSKLLR
ACGKIEGLERLRFTSPHPAEFTHDVIDAMAETPNVCPQLHMPLQSGSDKVLKEMRRSYRSKKFLGILEEVRAKIPHASIT
TDIIVGFPGETEEDFQETLNVVEKARFTSAYTFQYSPRPGTPAADYADQVPKEVVQDRYERLLALQERISTEENAKLIGT
EVELLVQASGGRKNDKTQRMTGRSRDGRLVHFDPQGHVDGDIRPGDVITTVVTEAKPFFLIADSGVLQHRRTKAGDMSAA
GKVPTTAPVGVGLGLPSIGSPAQKRSETSKSSGCGC

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=475, Percent_Identity=32.8421052631579, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI28872782, Length=475, Percent_Identity=32.8421052631579, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI93277076, Length=373, Percent_Identity=30.2949061662198, Blast_Score=162, Evalue=6e-40,
Organism=Escherichia coli, GI1786882, Length=423, Percent_Identity=39.0070921985816, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1787057, Length=457, Percent_Identity=30.4157549234136, Blast_Score=202, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17553146, Length=465, Percent_Identity=33.1182795698925, Blast_Score=239, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI71996771, Length=220, Percent_Identity=35, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI21356207, Length=435, Percent_Identity=35.632183908046, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI19922432, Length=401, Percent_Identity=29.426433915212, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_CORDI (Q6NGR0)

Other databases:

- EMBL:   BX248358
- RefSeq:   NP_939796.1
- ProteinModelPortal:   Q6NGR0
- SMR:   Q6NGR0
- GeneID:   2649719
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1448
- NMPDR:   fig|257309.1.peg.1387
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- PhylomeDB:   Q6NGR0
- ProtClustDB:   CLSK2760985
- BioCyc:   CDIP257309:DIP1448-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 56480; Mature: 56480

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNDIESHRVPNVGQGRSFEVRTFGCQMNVHDSERLSGLLEEAGYHAVADGEEPDLVVFN
CCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHCCCEEECCCCCCCEEEEE
TCAVRENADKRLYGTLGQLRSAKEKNPRMQIAVGGCLAQKDKDTVVAKAPWVDAVFGTHN
EEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHCCCCCCCEEEECCCHHHHHCCCC
MGALPSLLSRSEHNKRAEVEIVDSLEQFPSVLPAKRESAYAGWVSVSVGCNNTCTFCIVP
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEECEEEEEECCCCCEEEEEEC
SLRGKEVDRRPGDILAEVQALVDQGVSEVTLLGQNVNAYGVNFSDPDIQRDRFAFSKLLR
CCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHH
ACGKIEGLERLRFTSPHPAEFTHDVIDAMAETPNVCPQLHMPLQSGSDKVLKEMRRSYRS
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHHHHH
KKFLGILEEVRAKIPHASITTDIIVGFPGETEEDFQETLNVVEKARFTSAYTFQYSPRPG
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCC
TPAADYADQVPKEVVQDRYERLLALQERISTEENAKLIGTEVELLVQASGGRKNDKTQRM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEEEECCCCCCCHHHHH
TGRSRDGRLVHFDPQGHVDGDIRPGDVITTVVTEAKPFFLIADSGVLQHRRTKAGDMSAA
CCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHCCCCCCCCC
GKVPTTAPVGVGLGLPSIGSPAQKRSETSKSSGCGC
CCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCC
>Mature Secondary Structure
MFNDIESHRVPNVGQGRSFEVRTFGCQMNVHDSERLSGLLEEAGYHAVADGEEPDLVVFN
CCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHCCCEEECCCCCCCEEEEE
TCAVRENADKRLYGTLGQLRSAKEKNPRMQIAVGGCLAQKDKDTVVAKAPWVDAVFGTHN
EEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHCCCCCCCEEEECCCHHHHHCCCC
MGALPSLLSRSEHNKRAEVEIVDSLEQFPSVLPAKRESAYAGWVSVSVGCNNTCTFCIVP
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEECEEEEEECCCCCEEEEEEC
SLRGKEVDRRPGDILAEVQALVDQGVSEVTLLGQNVNAYGVNFSDPDIQRDRFAFSKLLR
CCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHH
ACGKIEGLERLRFTSPHPAEFTHDVIDAMAETPNVCPQLHMPLQSGSDKVLKEMRRSYRS
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHHHHH
KKFLGILEEVRAKIPHASITTDIIVGFPGETEEDFQETLNVVEKARFTSAYTFQYSPRPG
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCC
TPAADYADQVPKEVVQDRYERLLALQERISTEENAKLIGTEVELLVQASGGRKNDKTQRM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEEEECCCCCCCHHHHH
TGRSRDGRLVHFDPQGHVDGDIRPGDVITTVVTEAKPFFLIADSGVLQHRRTKAGDMSAA
CCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHCCCCCCCCC
GKVPTTAPVGVGLGLPSIGSPAQKRSETSKSSGCGC
CCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910