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The map label for this gene is pyrB

Identifier: 38233920

GI number: 38233920

Start: 1348820

End: 1349761

Strand: Reverse

Name: pyrB

Synonym: DIP1334

Alternate gene names: 38233920

Gene position: 1349761-1348820 (Counterclockwise)

Preceding gene: 38233921

Following gene: 38233919

Centisome position: 54.24

GC content: 49.04

Gene sequence:

>942_bases
GTGAAACATCTATTATCTATTGCTGACCTTAGCAAAGATGACATCATTGGTTTGATGGATGAAGCGGATCGTTTCCGAGA
AGCACTCTTAGGGCGTGATATTAAAAAACTTCCTACGCTTCGCGGTCGGACAATTTTTACCCTTTTCTACGAAAATTCGA
CGCGTACACGTTCATCATTCGAAACAGCTGGAAAATGGATGAGCGCTGATGTTATCAACATCTCTGCTGGCTCATCTTCT
GTCAAAAAGGGTGAATCCTTAAAAGACACTGGCCTGACGTTATCTGCGATTGGTGCAGATGCCATTGTTATGCGCCATTA
CTCATCAGGGGCGGCTGAACAACTAGCTCGTTGGGTTGCTCCAGGTGGAGTAGGGCCGAGTGTTATTAATGCTGGTGACG
GTGCGCATCAACATCCTACTCAAGCATTATTGGATGCTGTCACGATTCGTCAACGCTTAGGTGATATCGATGGTCGTAAG
GTTGTGATTGTTGGCGACTGCTTGCATTCGCGAGTGGTACGTTCCAACGTAGATCTTCTCACCACTTTGGGAGCCGAAGT
AGTCCTCGTTGGACCCCCCACGCTATTGCCTGCTGGTATTGATACATGGCCAGTTCGTTATTCCTATGACATGGACGCCG
AAATCTATGACGCAGATGTCGTCATGATGCTCCGAGTCCAGCAAGAACGGATGCATGGTGGATTCTTTCCGACACACCGC
GAATACGCCACCCTCTATGGCCTTTCAGCAGAGCGCGAACGAAAGCTCCAAGACCATACGATCGTGATGCATCCAGGCCC
AATGTTAAGGGGAATGGAAATCAACTTCGGAGTGGCCGATGCGCCTCGTACCGCAGTATTACAGCAGGTTAGTAATGGGG
TGCACACCCGAATGGCCGTTTTGTTCACTCTTATCGCTGGCCAAGACGCAGCGGCATTTTAA

Upstream 100 bases:

>100_bases
AGAATTTGCCTACTTCTCGTGGCGAAGATGTTCAAGTGTTTATCAAAGAAATTGATGGCCGAACAGCCGTTGTCTTAACT
CGTGGCACGGAGGAAGCATA

Downstream 100 bases:

>100_bases
AAAACTCAAGGAAGAGCAGCAACTATGAGTACTCAAAATTATCCAGCAACTGGCCGACTCGCCGAGCCGGAATATGGCAC
TCTGCTAGTCAAAAACGTGA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKHLLSIADLSKDDIIGLMDEADRFREALLGRDIKKLPTLRGRTIFTLFYENSTRTRSSFETAGKWMSADVINISAGSSS
VKKGESLKDTGLTLSAIGADAIVMRHYSSGAAEQLARWVAPGGVGPSVINAGDGAHQHPTQALLDAVTIRQRLGDIDGRK
VVIVGDCLHSRVVRSNVDLLTTLGAEVVLVGPPTLLPAGIDTWPVRYSYDMDAEIYDADVVMMLRVQQERMHGGFFPTHR
EYATLYGLSAERERKLQDHTIVMHPGPMLRGMEINFGVADAPRTAVLQQVSNGVHTRMAVLFTLIAGQDAAAF

Sequences:

>Translated_313_residues
MKHLLSIADLSKDDIIGLMDEADRFREALLGRDIKKLPTLRGRTIFTLFYENSTRTRSSFETAGKWMSADVINISAGSSS
VKKGESLKDTGLTLSAIGADAIVMRHYSSGAAEQLARWVAPGGVGPSVINAGDGAHQHPTQALLDAVTIRQRLGDIDGRK
VVIVGDCLHSRVVRSNVDLLTTLGAEVVLVGPPTLLPAGIDTWPVRYSYDMDAEIYDADVVMMLRVQQERMHGGFFPTHR
EYATLYGLSAERERKLQDHTIVMHPGPMLRGMEINFGVADAPRTAVLQQVSNGVHTRMAVLFTLIAGQDAAAF
>Mature_313_residues
MKHLLSIADLSKDDIIGLMDEADRFREALLGRDIKKLPTLRGRTIFTLFYENSTRTRSSFETAGKWMSADVINISAGSSS
VKKGESLKDTGLTLSAIGADAIVMRHYSSGAAEQLARWVAPGGVGPSVINAGDGAHQHPTQALLDAVTIRQRLGDIDGRK
VVIVGDCLHSRVVRSNVDLLTTLGAEVVLVGPPTLLPAGIDTWPVRYSYDMDAEIYDADVVMMLRVQQERMHGGFFPTHR
EYATLYGLSAERERKLQDHTIVMHPGPMLRGMEINFGVADAPRTAVLQQVSNGVHTRMAVLFTLIAGQDAAAF

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=314, Percent_Identity=35.9872611464968, Blast_Score=172, Evalue=4e-43,
Organism=Escherichia coli, GI2367364, Length=313, Percent_Identity=34.8242811501597, Blast_Score=135, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI193204318, Length=336, Percent_Identity=34.8214285714286, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6322331, Length=320, Percent_Identity=35.3125, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24642586, Length=323, Percent_Identity=33.7461300309598, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_CORDI (Q6NH13)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939687.1
- ProteinModelPortal:   Q6NH13
- SMR:   Q6NH13
- GeneID:   2648777
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1334
- NMPDR:   fig|257309.1.peg.1278
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   CDIP257309:DIP1334-MONOMER
- BRENDA:   2.1.3.2
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34111; Mature: 34111

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLLSIADLSKDDIIGLMDEADRFREALLGRDIKKLPTLRGRTIFTLFYENSTRTRSSF
CCCHHHHHCCCCCHHEEHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEEECCCCCHHHH
ETAGKWMSADVINISAGSSSVKKGESLKDTGLTLSAIGADAIVMRHYSSGAAEQLARWVA
HHHCCEEECEEEEEECCCCHHHCCCCCHHCCCEEEECCCCEEEEECCCCCHHHHHHHHHC
PGGVGPSVINAGDGAHQHPTQALLDAVTIRQRLGDIDGRKVVIVGDCLHSRVVRSNVDLL
CCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHCCCCEE
TTLGAEVVLVGPPTLLPAGIDTWPVRYSYDMDAEIYDADVVMMLRVQQERMHGGFFPTHR
ECCCCCEEEECCCCCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHHHHHHCCCCCCCCC
EYATLYGLSAERERKLQDHTIVMHPGPMLRGMEINFGVADAPRTAVLQQVSNGVHTRMAV
HHHHHCCCCHHHHHHHCCCEEEECCCCCEECEEEECCCCCCCHHHHHHHHHCCHHHHHHH
LFTLIAGQDAAAF
HHHHHCCCCCCCC
>Mature Secondary Structure
MKHLLSIADLSKDDIIGLMDEADRFREALLGRDIKKLPTLRGRTIFTLFYENSTRTRSSF
CCCHHHHHCCCCCHHEEHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEEECCCCCHHHH
ETAGKWMSADVINISAGSSSVKKGESLKDTGLTLSAIGADAIVMRHYSSGAAEQLARWVA
HHHCCEEECEEEEEECCCCHHHCCCCCHHCCCEEEECCCCEEEEECCCCCHHHHHHHHHC
PGGVGPSVINAGDGAHQHPTQALLDAVTIRQRLGDIDGRKVVIVGDCLHSRVVRSNVDLL
CCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHCCCCEE
TTLGAEVVLVGPPTLLPAGIDTWPVRYSYDMDAEIYDADVVMMLRVQQERMHGGFFPTHR
ECCCCCEEEECCCCCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHHHHHHCCCCCCCCC
EYATLYGLSAERERKLQDHTIVMHPGPMLRGMEINFGVADAPRTAVLQQVSNGVHTRMAV
HHHHHCCCCHHHHHHHCCCEEEECCCCCEECEEEECCCCCCCHHHHHHHHHCCHHHHHHH
LFTLIAGQDAAAF
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910