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The map label for this gene is menF [H]

Identifier: 38233704

GI number: 38233704

Start: 1096756

End: 1097889

Strand: Reverse

Name: menF [H]

Synonym: DIP1114

Alternate gene names: 38233704

Gene position: 1097889-1096756 (Counterclockwise)

Preceding gene: 38233707

Following gene: 38233691

Centisome position: 44.12

GC content: 54.67

Gene sequence:

>1134_bases
ATGTGTGCCCATCGCCCCGCTACTGCCCCCGATTTTTTACTCTCCCGTAGCCATGGCTCTATCCGTACCCAAGGCGCGGT
AGAGACGTTTTCCGACCCCGGGAAAGCGGTAGAAGCCTTACGTAGCGGGCGCGTAGAGCTCGTTGTTGGCGCCCTTGCTT
TTCGACGCGACCACGCCTGCGCCCTTACGGTTCCCCGCTCCGTCATACGCGAGGAGGGCCCCTTAGAGCCCCACGCATAT
TACCGACAAGGACCCGGCAGCATTGTGAATGCTCAAGTAGAAAAGCTTGTCCCATCACTCGATAAACATCATGCCCGCGT
CGAAGCAGCGATCAATACAATAAAGTCCACATCCTTGAAAAAAGTCGTTTTAGCACGCGCAGTGGATATGACGTTCTCCC
CTGCCGTCGATCCACGACTTATCGCTGCACGCCTGATTGATCTTTCCCGCAACCGCGATGGTTTCATCGCAGACCTCACC
CCTGCCGGCGAAGATTTTGCAGGAGCAATGCTTGTAGGCTCATCACCAGAAGTACTTATCAAGAAACAAGGCTCAACCGT
GACGGCGTATCCCCTCGCTGGTTCTGCACCCAGAAGCGCGGATGCGGTCACAGATGCCACCAATGCCGAACTTTTACGAC
GTTCCCACAAAGATCTACAAGAACACTCGTACGTTGTAGAGCATCTTCGCAATGTTTTAGAGCCACTATGTTCAAAGCTC
AGCATCCCCGCAAACCCTGAGCTCACCAAGACCAATGAAATGTGGCACCTTGCCACTCCTGTTGTTGGAACCTTAAAGGA
CGCATCACTTACTGCACTTGAATTAGCAATGCGCACTCATCCCACCCCTGCCATCTGCGGCACTCCGACTGATGCGGCCG
AAGAGCTCATCAGCATCGCAGAAGGCGATCGCCGGTTCTATGCAGGAACCGTGGGCTGGGCAGACAACCACGGCGATGGT
GAATTCATGGTGTCTATCCGGTGTGCCGAGGTAAGCAGTGACGGCACCTCCGCACGAGCATGGGCAGGCGGAGGAATCGT
CGCAGAATCTGATGCGGCCGCAGAAGTCGAAGAAACAACTGCGAAACTTCGCACCATCATGAAAGCTTTAGGCATTACTT
CTTTCGACGCTTAA

Upstream 100 bases:

>100_bases
ACCATACCAACATGTGGCGTGCCGGTCGGCGACGGACAAAAACGTACACGAACATCAGACATAATGTTCATCGTAGACTA
GTGCACTAGACTGGCAAGAC

Downstream 100 bases:

>100_bases
GTACCAAGACGTCATCAAGATGGTGATGGTGGCCATGCCCTCCTACGATCTCGCGATGGCGAGTTTCGTGGACCACGATT
TCCCATGCACCACTAGGAGC

Product: isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MCAHRPATAPDFLLSRSHGSIRTQGAVETFSDPGKAVEALRSGRVELVVGALAFRRDHACALTVPRSVIREEGPLEPHAY
YRQGPGSIVNAQVEKLVPSLDKHHARVEAAINTIKSTSLKKVVLARAVDMTFSPAVDPRLIAARLIDLSRNRDGFIADLT
PAGEDFAGAMLVGSSPEVLIKKQGSTVTAYPLAGSAPRSADAVTDATNAELLRRSHKDLQEHSYVVEHLRNVLEPLCSKL
SIPANPELTKTNEMWHLATPVVGTLKDASLTALELAMRTHPTPAICGTPTDAAEELISIAEGDRRFYAGTVGWADNHGDG
EFMVSIRCAEVSSDGTSARAWAGGGIVAESDAAAEVEETTAKLRTIMKALGITSFDA

Sequences:

>Translated_377_residues
MCAHRPATAPDFLLSRSHGSIRTQGAVETFSDPGKAVEALRSGRVELVVGALAFRRDHACALTVPRSVIREEGPLEPHAY
YRQGPGSIVNAQVEKLVPSLDKHHARVEAAINTIKSTSLKKVVLARAVDMTFSPAVDPRLIAARLIDLSRNRDGFIADLT
PAGEDFAGAMLVGSSPEVLIKKQGSTVTAYPLAGSAPRSADAVTDATNAELLRRSHKDLQEHSYVVEHLRNVLEPLCSKL
SIPANPELTKTNEMWHLATPVVGTLKDASLTALELAMRTHPTPAICGTPTDAAEELISIAEGDRRFYAGTVGWADNHGDG
EFMVSIRCAEVSSDGTSARAWAGGGIVAESDAAAEVEETTAKLRTIMKALGITSFDA
>Mature_377_residues
MCAHRPATAPDFLLSRSHGSIRTQGAVETFSDPGKAVEALRSGRVELVVGALAFRRDHACALTVPRSVIREEGPLEPHAY
YRQGPGSIVNAQVEKLVPSLDKHHARVEAAINTIKSTSLKKVVLARAVDMTFSPAVDPRLIAARLIDLSRNRDGFIADLT
PAGEDFAGAMLVGSSPEVLIKKQGSTVTAYPLAGSAPRSADAVTDATNAELLRRSHKDLQEHSYVVEHLRNVLEPLCSKL
SIPANPELTKTNEMWHLATPVVGTLKDASLTALELAMRTHPTPAICGTPTDAAEELISIAEGDRRFYAGTVGWADNHGDG
EFMVSIRCAEVSSDGTSARAWAGGGIVAESDAAAEVEETTAKLRTIMKALGITSFDA

Specific function: Might be involved in the biosynthesis of menaquinone. Converts chorismate to isochorismate [H]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=333, Percent_Identity=34.8348348348348, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI87082077, Length=288, Percent_Identity=29.5138888888889, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787518, Length=212, Percent_Identity=33.9622641509434, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788114, Length=207, Percent_Identity=30.9178743961353, Blast_Score=69, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320935, Length=204, Percent_Identity=34.3137254901961, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 40171; Mature: 40171

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCAHRPATAPDFLLSRSHGSIRTQGAVETFSDPGKAVEALRSGRVELVVGALAFRRDHAC
CCCCCCCCCHHHHHCCCCCCEEECCCHHHHCCCHHHHHHHHCCCEEEEEHHHHHHCCCCE
ALTVPRSVIREEGPLEPHAYYRQGPGSIVNAQVEKLVPSLDKHHARVEAAINTIKSTSLK
EEECCHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVVLARAVDMTFSPAVDPRLIAARLIDLSRNRDGFIADLTPAGEDFAGAMLVGSSPEVLI
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEECCCCCEEE
KKQGSTVTAYPLAGSAPRSADAVTDATNAELLRRSHKDLQEHSYVVEHLRNVLEPLCSKL
EECCCEEEEECCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SIPANPELTKTNEMWHLATPVVGTLKDASLTALELAMRTHPTPAICGTPTDAAEELISIA
CCCCCCCCCCCCCEEEECCCCHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
EGDRRFYAGTVGWADNHGDGEFMVSIRCAEVSSDGTSARAWAGGGIVAESDAAAEVEETT
CCCCEEEEEECCCCCCCCCCEEEEEEEEEEECCCCCCCEEECCCCEEECCCCHHHHHHHH
AKLRTIMKALGITSFDA
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MCAHRPATAPDFLLSRSHGSIRTQGAVETFSDPGKAVEALRSGRVELVVGALAFRRDHAC
CCCCCCCCCHHHHHCCCCCCEEECCCHHHHCCCHHHHHHHHCCCEEEEEHHHHHHCCCCE
ALTVPRSVIREEGPLEPHAYYRQGPGSIVNAQVEKLVPSLDKHHARVEAAINTIKSTSLK
EEECCHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVVLARAVDMTFSPAVDPRLIAARLIDLSRNRDGFIADLTPAGEDFAGAMLVGSSPEVLI
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEECCCCCEEE
KKQGSTVTAYPLAGSAPRSADAVTDATNAELLRRSHKDLQEHSYVVEHLRNVLEPLCSKL
EECCCEEEEECCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SIPANPELTKTNEMWHLATPVVGTLKDASLTALELAMRTHPTPAICGTPTDAAEELISIA
CCCCCCCCCCCCCEEEECCCCHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
EGDRRFYAGTVGWADNHGDGEFMVSIRCAEVSSDGTSARAWAGGGIVAESDAAAEVEETT
CCCCEEEEEECCCCCCCCCCEEEEEEEEEEECCCCCCCEEECCCCEEECCCCHHHHHHHH
AKLRTIMKALGITSFDA
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]