Click here to switch to the map view.

The map label for this gene is putP [H]

Identifier: 38233617

GI number: 38233617

Start: 997318

End: 998883

Strand: Reverse

Name: putP [H]

Synonym: DIP1022

Alternate gene names: 38233617

Gene position: 998883-997318 (Counterclockwise)

Preceding gene: 38233622

Following gene: 38233616

Centisome position: 40.14

GC content: 50.64

Gene sequence:

>1566_bases
ATGACCGAACATACGTGGTTCATCGTCGCCATCATCATTTATATGGCGGTGATGTTAGCTATCGGTTATTGGAGCTACCG
CAAAACTGACCAGTATGACGACTATGTGCTTGCGGGGCGCGACCTCAACCCCTTTGTTGCTGCACTATCTGCCGGTGCCT
CTGATATGTCCGGTTGGCTGCTCATGGGTTTGCCTGGCGCGCTGTTTGTCACCGGCATGTCGGAACTGTGGATCGCCGTA
GGTTTGTTGATTGGTGCATGGGCTAATTGGCGCTGGGTGGCACCTCGCTTGCGCTCCTACTCGGAGGTTGCCCAGAATTC
GATTACACTTCCGTCGTTTTTTGAAAATCGCCTTGAGGATTCCTCGCGAATCCTTCGCATTGTCAGCTCGTTGATCATCA
TTGTGTTTTTCACCTTCTATGTATCTTCGGGCATGGTTTCAGGTGGCCGCTATTTCGAGTCAACATTTGGCGGTGCGTAT
CTCGACGGCATGCTGATCGTTGCTTGTATCACGGTCATTTACACCTTTATCGGTGGTTTTTTGGCTGTGTCCTACACGGA
TGCAGTTCAGGGTTCCATCATGTTTGTGTCGCTCGTTCTCGTTCCAGTAGCGGCATTGTTATATCTGGATGATCCCAGTT
CAATTTGGACATGGGCAACATCGCATGACTACGGCCCGTACATGGATGGCGTAGGTAATCCGCATTATTTCTCAATGATT
TCTGGTGTTTCGGTTGCTGCGGTGATCGGTAACCTTGCTTGGGGTTTGGGCTATTTTGGCCAGCCCCACATCATCGTCCG
TTTTATGGCTCTGCGTAAGCCATCAGATGCGAGCCAAGCACGGTGGATCGGTACAGCATGGATGTTGTTGTCCATTTTGG
GCGCAACGTTTACCGCGATTATTGGCACGGCATTTTTTGGCCAAAATCCTCATGTCGAGGTTGTTGATAAGAAAGCATAC
GAAACAATTTTCTTGGACATGGGTCGCGTACTTTTCCACCCGCTTATCGGCGGTTTGATTCTTACCGCGGTTCTAGCAGC
GATAATGTCGACGATCGCTTCACAGCTCCTAGTCACCTCCACCTCGTTGATCGAAGACCTATTTAAGGCCTTTAAAAAGA
ACCTTCCCAGCGAGACTGTGATGATTAACCTTTCTCGTACTGCCGTCATGGCAGTAGCCATTGTGGCCGGCGTATTGGCT
ATCGACCCCCAAGATTCCATTCTGAACCTAGTGGGCTTTGCATGGGCTGGTTTTGGTTCAGCGTTTGGTCCGCTCGTGCT
GCTCACTTTGTACTGGAAGCGTTTGAATTTCGCAGGCGCTATCTCAGGCATGGTTACCGGCGCTGTTGTGTCGTTTGCTT
GGGGCATGTCGCCTCTAGGAGATAGCTTGTACGAAATCGTGCCTGGCTTCTTCAGCGGATTGTTCGTGATGGTTGTGGTG
TCACTAGCCACCCCTGCCCCACCTGCATCTGTACGTTCTGCTTTTGATCGCGCCACTAAGCTGACAAAAGTTTCACAGGC
ACACCCAGAGCTAGAGGTCAGCGAAGTTCAAGAAAAGTTGCTCTAA

Upstream 100 bases:

>100_bases
AATTGTTTTTCGTCGCGTTCGTCGAGCACACGGTCCTTGTCTCAGATATTTCATCTCCGTGCGTCGTTCGATCAGGCACC
ACGTGAGGAGGCTAGAGAGG

Downstream 100 bases:

>100_bases
CAGGGAACCAAATAATGCGCTTTATACGTCCAAACATGTATGAAGCGCATTTTCTTTACCCTCCTAGTCGCGATGACGTG
TTTCGCGGTGTATTTGTCGA

Product: sodium/proline symporter

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MTEHTWFIVAIIIYMAVMLAIGYWSYRKTDQYDDYVLAGRDLNPFVAALSAGASDMSGWLLMGLPGALFVTGMSELWIAV
GLLIGAWANWRWVAPRLRSYSEVAQNSITLPSFFENRLEDSSRILRIVSSLIIIVFFTFYVSSGMVSGGRYFESTFGGAY
LDGMLIVACITVIYTFIGGFLAVSYTDAVQGSIMFVSLVLVPVAALLYLDDPSSIWTWATSHDYGPYMDGVGNPHYFSMI
SGVSVAAVIGNLAWGLGYFGQPHIIVRFMALRKPSDASQARWIGTAWMLLSILGATFTAIIGTAFFGQNPHVEVVDKKAY
ETIFLDMGRVLFHPLIGGLILTAVLAAIMSTIASQLLVTSTSLIEDLFKAFKKNLPSETVMINLSRTAVMAVAIVAGVLA
IDPQDSILNLVGFAWAGFGSAFGPLVLLTLYWKRLNFAGAISGMVTGAVVSFAWGMSPLGDSLYEIVPGFFSGLFVMVVV
SLATPAPPASVRSAFDRATKLTKVSQAHPELEVSEVQEKLL

Sequences:

>Translated_521_residues
MTEHTWFIVAIIIYMAVMLAIGYWSYRKTDQYDDYVLAGRDLNPFVAALSAGASDMSGWLLMGLPGALFVTGMSELWIAV
GLLIGAWANWRWVAPRLRSYSEVAQNSITLPSFFENRLEDSSRILRIVSSLIIIVFFTFYVSSGMVSGGRYFESTFGGAY
LDGMLIVACITVIYTFIGGFLAVSYTDAVQGSIMFVSLVLVPVAALLYLDDPSSIWTWATSHDYGPYMDGVGNPHYFSMI
SGVSVAAVIGNLAWGLGYFGQPHIIVRFMALRKPSDASQARWIGTAWMLLSILGATFTAIIGTAFFGQNPHVEVVDKKAY
ETIFLDMGRVLFHPLIGGLILTAVLAAIMSTIASQLLVTSTSLIEDLFKAFKKNLPSETVMINLSRTAVMAVAIVAGVLA
IDPQDSILNLVGFAWAGFGSAFGPLVLLTLYWKRLNFAGAISGMVTGAVVSFAWGMSPLGDSLYEIVPGFFSGLFVMVVV
SLATPAPPASVRSAFDRATKLTKVSQAHPELEVSEVQEKLL
>Mature_520_residues
TEHTWFIVAIIIYMAVMLAIGYWSYRKTDQYDDYVLAGRDLNPFVAALSAGASDMSGWLLMGLPGALFVTGMSELWIAVG
LLIGAWANWRWVAPRLRSYSEVAQNSITLPSFFENRLEDSSRILRIVSSLIIIVFFTFYVSSGMVSGGRYFESTFGGAYL
DGMLIVACITVIYTFIGGFLAVSYTDAVQGSIMFVSLVLVPVAALLYLDDPSSIWTWATSHDYGPYMDGVGNPHYFSMIS
GVSVAAVIGNLAWGLGYFGQPHIIVRFMALRKPSDASQARWIGTAWMLLSILGATFTAIIGTAFFGQNPHVEVVDKKAYE
TIFLDMGRVLFHPLIGGLILTAVLAAIMSTIASQLLVTSTSLIEDLFKAFKKNLPSETVMINLSRTAVMAVAIVAGVLAI
DPQDSILNLVGFAWAGFGSAFGPLVLLTLYWKRLNFAGAISGMVTGAVVSFAWGMSPLGDSLYEIVPGFFSGLFVMVVVS
LATPAPPASVRSAFDRATKLTKVSQAHPELEVSEVQEKLL

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=508, Percent_Identity=46.4566929133858, Blast_Score=434, Evalue=1e-122,
Organism=Escherichia coli, GI1787251, Length=501, Percent_Identity=45.1097804391218, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI87082237, Length=456, Percent_Identity=25.6578947368421, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790503, Length=455, Percent_Identity=25.0549450549451, Blast_Score=102, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17539284, Length=527, Percent_Identity=23.719165085389, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24640370, Length=384, Percent_Identity=23.9583333333333, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI221459584, Length=376, Percent_Identity=22.3404255319149, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 56522; Mature: 56390

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHTWFIVAIIIYMAVMLAIGYWSYRKTDQYDDYVLAGRDLNPFVAALSAGASDMSGWL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCCEE
LMGLPGALFVTGMSELWIAVGLLIGAWANWRWVAPRLRSYSEVAQNSITLPSFFENRLED
EECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHH
SSRILRIVSSLIIIVFFTFYVSSGMVSGGRYFESTFGGAYLDGMLIVACITVIYTFIGGF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LAVSYTDAVQGSIMFVSLVLVPVAALLYLDDPSSIWTWATSHDYGPYMDGVGNPHYFSMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHH
SGVSVAAVIGNLAWGLGYFGQPHIIVRFMALRKPSDASQARWIGTAWMLLSILGATFTAI
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IGTAFFGQNPHVEVVDKKAYETIFLDMGRVLFHPLIGGLILTAVLAAIMSTIASQLLVTS
HHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSLIEDLFKAFKKNLPSETVMINLSRTAVMAVAIVAGVLAIDPQDSILNLVGFAWAGFGS
HHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCH
AFGPLVLLTLYWKRLNFAGAISGMVTGAVVSFAWGMSPLGDSLYEIVPGFFSGLFVMVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
SLATPAPPASVRSAFDRATKLTKVSQAHPELEVSEVQEKLL
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC
>Mature Secondary Structure 
TEHTWFIVAIIIYMAVMLAIGYWSYRKTDQYDDYVLAGRDLNPFVAALSAGASDMSGWL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCCEE
LMGLPGALFVTGMSELWIAVGLLIGAWANWRWVAPRLRSYSEVAQNSITLPSFFENRLED
EECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHH
SSRILRIVSSLIIIVFFTFYVSSGMVSGGRYFESTFGGAYLDGMLIVACITVIYTFIGGF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LAVSYTDAVQGSIMFVSLVLVPVAALLYLDDPSSIWTWATSHDYGPYMDGVGNPHYFSMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHH
SGVSVAAVIGNLAWGLGYFGQPHIIVRFMALRKPSDASQARWIGTAWMLLSILGATFTAI
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IGTAFFGQNPHVEVVDKKAYETIFLDMGRVLFHPLIGGLILTAVLAAIMSTIASQLLVTS
HHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSLIEDLFKAFKKNLPSETVMINLSRTAVMAVAIVAGVLAIDPQDSILNLVGFAWAGFGS
HHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCH
AFGPLVLLTLYWKRLNFAGAISGMVTGAVVSFAWGMSPLGDSLYEIVPGFFSGLFVMVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
SLATPAPPASVRSAFDRATKLTKVSQAHPELEVSEVQEKLL
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]