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The map label for this gene is folD [H]

Identifier: 37680575

GI number: 37680575

Start: 2426525

End: 2427382

Strand: Direct

Name: folD [H]

Synonym: VV2391

Alternate gene names: 37680575

Gene position: 2426525-2427382 (Clockwise)

Preceding gene: 37680567

Following gene: 37680576

Centisome position: 72.34

GC content: 51.52

Gene sequence:

>858_bases
ATGACTGCTCAAAATATCGATGGAACTCTCATTTCCCAAACTGTTCGATCCGAAGTCGCTGCTCGCGTAAAAGCTCGTGT
AGCTGCGGGATTACGTGCGCCTGGCTTAGCCGTTGTGTTGGTTGGCGAAGATCCAGCGTCTCAAGTTTACGTTGGCAGCA
AGCGTCGCGCGTGTGAGGAAGTGGGCTTTGTCTCTAAATCGTTTGATCTACCCGCTTCAACTTCAGAAGAAGAACTGCTG
GCGCTGATCGATGAACTCAATAATGATGCTGAAATCGACGGTATTCTGGTTCAACTTCCTCTTCCTGCGGGCATTGACAG
CACGCACGTGCTAGAAAGCATTCATCCAGAGAAAGATGTGGATGGTTTCCACCCGTACAACGTTGGCCGCTTAGCGCAGC
GCATCCCTAAACTGCGTTCGTGCACACCAAAAGGCATCATTACGCTGCTTGAGCGTTACAACATCGCACTACGTGGTAAG
CATGCGGTGATCGTGGGTGCATCAAACATTGTTGGTCGCCCAATGACACTCGAGCTACTGCTGGCAGGTTGTACCACGAC
CACCTGTCACCGCTTCACGAAAGATCTTGAAGGCCATGTTCGCCAAGCTGATGTGCTCGTGGTTGCGGTCGGTAAGCCAA
ATTTCATTCCTGGTGAATGGGTCAAAGAAGGCGCGATCGTGGTCGATGTCGGCATCAACCGCCTTGAATCGGGTAAGCTG
ATTGGTGATGTGGAATATAATAAGGCTCGTGAACGCGCCAGCTTCATTACTCCAGTGCCCGGTGGTGTTGGCCCAATGAC
TGTGGCCAGCCTCATCGAAAACACCATGCTTGCTTGCGAGCAATACCACACCAAATAA

Upstream 100 bases:

>100_bases
TTAAAAACGACCCAAAGCAAACGTTTGCGTATAGATATTTATTTGATCATTTGCGACAATACGCGCCAAGTTTTTGCCAT
TCGTATTCTTAAGGAAAGTC

Downstream 100 bases:

>100_bases
GTCCTCTTCGGCGGGTCAAATCTTGGCCTGCCGAGACTCAAACTGGTTAAAACAGGCAGTTCCTGTTAGACTTTGCCGCC
TGTTTCATCTCCCCTTTTTG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTAQNIDGTLISQTVRSEVAARVKARVAAGLRAPGLAVVLVGEDPASQVYVGSKRRACEEVGFVSKSFDLPASTSEEELL
ALIDELNNDAEIDGILVQLPLPAGIDSTHVLESIHPEKDVDGFHPYNVGRLAQRIPKLRSCTPKGIITLLERYNIALRGK
HAVIVGASNIVGRPMTLELLLAGCTTTTCHRFTKDLEGHVRQADVLVVAVGKPNFIPGEWVKEGAIVVDVGINRLESGKL
IGDVEYNKARERASFITPVPGGVGPMTVASLIENTMLACEQYHTK

Sequences:

>Translated_285_residues
MTAQNIDGTLISQTVRSEVAARVKARVAAGLRAPGLAVVLVGEDPASQVYVGSKRRACEEVGFVSKSFDLPASTSEEELL
ALIDELNNDAEIDGILVQLPLPAGIDSTHVLESIHPEKDVDGFHPYNVGRLAQRIPKLRSCTPKGIITLLERYNIALRGK
HAVIVGASNIVGRPMTLELLLAGCTTTTCHRFTKDLEGHVRQADVLVVAVGKPNFIPGEWVKEGAIVVDVGINRLESGKL
IGDVEYNKARERASFITPVPGGVGPMTVASLIENTMLACEQYHTK
>Mature_284_residues
TAQNIDGTLISQTVRSEVAARVKARVAAGLRAPGLAVVLVGEDPASQVYVGSKRRACEEVGFVSKSFDLPASTSEEELLA
LIDELNNDAEIDGILVQLPLPAGIDSTHVLESIHPEKDVDGFHPYNVGRLAQRIPKLRSCTPKGIITLLERYNIALRGKH
AVIVGASNIVGRPMTLELLLAGCTTTTCHRFTKDLEGHVRQADVLVVAVGKPNFIPGEWVKEGAIVVDVGINRLESGKLI
GDVEYNKARERASFITPVPGGVGPMTVASLIENTMLACEQYHTK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=300, Percent_Identity=42.3333333333333, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI94721354, Length=291, Percent_Identity=44.6735395189003, Blast_Score=221, Evalue=4e-58,
Organism=Homo sapiens, GI222418558, Length=291, Percent_Identity=42.2680412371134, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI36796743, Length=209, Percent_Identity=29.6650717703349, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1786741, Length=283, Percent_Identity=73.8515901060071, Blast_Score=442, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17568735, Length=289, Percent_Identity=40.4844290657439, Blast_Score=183, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=42.1404682274247, Blast_Score=229, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6319558, Length=285, Percent_Identity=41.0526315789474, Blast_Score=223, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6322933, Length=311, Percent_Identity=25.4019292604502, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=47.9591836734694, Blast_Score=251, Evalue=3e-67,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=47.9591836734694, Blast_Score=251, Evalue=4e-67,
Organism=Drosophila melanogaster, GI62472483, Length=291, Percent_Identity=41.2371134020619, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI45551871, Length=291, Percent_Identity=41.2371134020619, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24645718, Length=291, Percent_Identity=41.2371134020619, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI17137370, Length=291, Percent_Identity=41.2371134020619, Blast_Score=208, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30619; Mature: 30487

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQNIDGTLISQTVRSEVAARVKARVAAGLRAPGLAVVLVGEDPASQVYVGSKRRACEE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCHHHHHHH
VGFVSKSFDLPASTSEEELLALIDELNNDAEIDGILVQLPLPAGIDSTHVLESIHPEKDV
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCC
DGFHPYNVGRLAQRIPKLRSCTPKGIITLLERYNIALRGKHAVIVGASNIVGRPMTLELL
CCCCCCCHHHHHHHCHHHHCCCHHHHHHHHHHCCEEEECCEEEEEECHHHCCCCCEEEHH
LAGCTTTTCHRFTKDLEGHVRQADVLVVAVGKPNFIPGEWVKEGAIVVDVGINRLESGKL
HHCCCHHHHHHHHHHHHHHCEEEEEEEEEECCCCCCCCHHHHCCCEEEEECCCCCCCCCE
IGDVEYNKARERASFITPVPGGVGPMTVASLIENTMLACEQYHTK
EEECCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAQNIDGTLISQTVRSEVAARVKARVAAGLRAPGLAVVLVGEDPASQVYVGSKRRACEE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCHHHHHHH
VGFVSKSFDLPASTSEEELLALIDELNNDAEIDGILVQLPLPAGIDSTHVLESIHPEKDV
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCC
DGFHPYNVGRLAQRIPKLRSCTPKGIITLLERYNIALRGKHAVIVGASNIVGRPMTLELL
CCCCCCCHHHHHHHCHHHHCCCHHHHHHHHHHCCEEEECCEEEEEECHHHCCCCCEEEHH
LAGCTTTTCHRFTKDLEGHVRQADVLVVAVGKPNFIPGEWVKEGAIVVDVGINRLESGKL
HHCCCHHHHHHHHHHHHHHCEEEEEEEEEECCCCCCCCHHHHCCCEEEEECCCCCCCCCE
IGDVEYNKARERASFITPVPGGVGPMTVASLIENTMLACEQYHTK
EEECCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA