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The map label for this gene is dosC [H]

Identifier: 37680564

GI number: 37680564

Start: 2412163

End: 2413149

Strand: Direct

Name: dosC [H]

Synonym: VV2380

Alternate gene names: 37680564

Gene position: 2412163-2413149 (Clockwise)

Preceding gene: 37680560

Following gene: 37680567

Centisome position: 71.91

GC content: 48.13

Gene sequence:

>987_bases
ATGGTAGGAGTACGCTGCATGGTAGAACAGTCCATTTTTTCGCGTGTTATCGCCAACAACCCGGCGGTCATTCACCACAT
TTTTGATGCGCTTCCTGAACCAACCTTCTTGATCAATGGCGATGGCTATTACATTGAGGCTTGGGGTGGCACCGACACTT
CTCGTCACCACGACCCAGCTAATGTAATTGGCTTAAACCAGTTCGAAGTGCTCCCTCACGATAAAGCTCGGTGGTTTTCT
GACGTGATCACGCAAGTGATCGAATCCCAAGCTCCACAAGAGTTGGAATATGCGCTGCATCCGAAAGATCTCAACTGTTT
TGAAGGTGTCGCGGGGCCGACTGAGTTGCAATATTTTAGTGCCTTTGTCATTCCGGTATTTGGAGAAAACAAGGTGCTGT
GGACCGTGCGCAACATCACTGAATACAAAACGGCGCTGCAGAAACTGGAACGGCAACAACTGGAGTTGGAGAAACTCTCC
TGCCTTGATCATTTAACTCAACTCTACAATCGCTATGCGCTTGAAGTGCACCTTCCTAGCGCCATTGCCAATACCGCACA
ATCTGGCAAAAGTGCCGCGCTCTTTATGGTTGATATTGATTGTTTTAAAGATCTCAACGACACCTATGGTCATCTTACTG
GCGACAAAGCATTGCGTATGGTCTCGCTGGCGCTAAAACACTGGAGTGGCCACAATGGCATTTGCTTTCGTTATGGCGGC
GATGAGTTCTTGGTTTTCATCCAAGATATTTCGCAAGAGGAATGCCAGTCTATGGCGCATTCACTGCAACAGTCGATTCA
GAAACTGGCCATTCCTAATCAACATTCTCATGTGGCCGACACCTTAACCCTCACCATCGGCATTCAGTTTTATCCCAGCC
TAACGGGCGACAGCCAAGACCTCGAGCAGTTTATTTCCAAAGCGGATAAAGCGCTGTTTAACGCCAAAAACACCCAACGT
GGCACCGTTCACTTCCTCTCTCATTAA

Upstream 100 bases:

>100_bases
CCCACCTATCAATGAATAGATCGAGCTTGTACTACAAATAAGACAAATATTTTGTTCCCCGTCACGCGGCGCTCACGCTA
TTGAGCTATAAGTTAATGAA

Downstream 100 bases:

>100_bases
TCATTGGGATCTGCCGTGCAAAAAAAAAGAGAGCCATTTGTTTGGCTCTCTTTGGGTCACGCGTCTATTGGGGTAGTCAC
GTTGCGTTAAATCATTGACC

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MVGVRCMVEQSIFSRVIANNPAVIHHIFDALPEPTFLINGDGYYIEAWGGTDTSRHHDPANVIGLNQFEVLPHDKARWFS
DVITQVIESQAPQELEYALHPKDLNCFEGVAGPTELQYFSAFVIPVFGENKVLWTVRNITEYKTALQKLERQQLELEKLS
CLDHLTQLYNRYALEVHLPSAIANTAQSGKSAALFMVDIDCFKDLNDTYGHLTGDKALRMVSLALKHWSGHNGICFRYGG
DEFLVFIQDISQEECQSMAHSLQQSIQKLAIPNQHSHVADTLTLTIGIQFYPSLTGDSQDLEQFISKADKALFNAKNTQR
GTVHFLSH

Sequences:

>Translated_328_residues
MVGVRCMVEQSIFSRVIANNPAVIHHIFDALPEPTFLINGDGYYIEAWGGTDTSRHHDPANVIGLNQFEVLPHDKARWFS
DVITQVIESQAPQELEYALHPKDLNCFEGVAGPTELQYFSAFVIPVFGENKVLWTVRNITEYKTALQKLERQQLELEKLS
CLDHLTQLYNRYALEVHLPSAIANTAQSGKSAALFMVDIDCFKDLNDTYGHLTGDKALRMVSLALKHWSGHNGICFRYGG
DEFLVFIQDISQEECQSMAHSLQQSIQKLAIPNQHSHVADTLTLTIGIQFYPSLTGDSQDLEQFISKADKALFNAKNTQR
GTVHFLSH
>Mature_328_residues
MVGVRCMVEQSIFSRVIANNPAVIHHIFDALPEPTFLINGDGYYIEAWGGTDTSRHHDPANVIGLNQFEVLPHDKARWFS
DVITQVIESQAPQELEYALHPKDLNCFEGVAGPTELQYFSAFVIPVFGENKVLWTVRNITEYKTALQKLERQQLELEKLS
CLDHLTQLYNRYALEVHLPSAIANTAQSGKSAALFMVDIDCFKDLNDTYGHLTGDKALRMVSLALKHWSGHNGICFRYGG
DEFLVFIQDISQEECQSMAHSLQQSIQKLAIPNQHSHVADTLTLTIGIQFYPSLTGDSQDLEQFISKADKALFNAKNTQR
GTVHFLSH

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=200, Percent_Identity=30.5, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787541, Length=178, Percent_Identity=34.8314606741573, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1786584, Length=181, Percent_Identity=29.8342541436464, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787816, Length=189, Percent_Identity=29.6296296296296, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081881, Length=224, Percent_Identity=28.125, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788956, Length=185, Percent_Identity=30.2702702702703, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=29.4478527607362, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787802, Length=158, Percent_Identity=27.8481012658228, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081974, Length=119, Percent_Identity=31.0924369747899, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1788085, Length=167, Percent_Identity=27.5449101796407, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788381, Length=190, Percent_Identity=25.2631578947368, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI87082007, Length=166, Percent_Identity=31.9277108433735, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 36921; Mature: 36921

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGVRCMVEQSIFSRVIANNPAVIHHIFDALPEPTFLINGDGYYIEAWGGTDTSRHHDPA
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCCCCCCCC
NVIGLNQFEVLPHDKARWFSDVITQVIESQAPQELEYALHPKDLNCFEGVAGPTELQYFS
CEECCCCEEECCCHHHHHHHHHHHHHHHCCCCHHHHHEECCCCCCHHHCCCCCHHHHEEE
AFVIPVFGENKVLWTVRNITEYKTALQKLERQQLELEKLSCLDHLTQLYNRYALEVHLPS
EEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCH
AIANTAQSGKSAALFMVDIDCFKDLNDTYGHLTGDKALRMVSLALKHWSGHNGICFRYGG
HHHHHHCCCCCEEEEEEEHHHHHCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECC
DEFLVFIQDISQEECQSMAHSLQQSIQKLAIPNQHSHVADTLTLTIGIQFYPSLTGDSQD
CEEEEEEHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEEEEEEECCCCCCHHH
LEQFISKADKALFNAKNTQRGTVHFLSH
HHHHHHHHHHHHHCCCCCCCCEEEEECC
>Mature Secondary Structure
MVGVRCMVEQSIFSRVIANNPAVIHHIFDALPEPTFLINGDGYYIEAWGGTDTSRHHDPA
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCCCCCCCC
NVIGLNQFEVLPHDKARWFSDVITQVIESQAPQELEYALHPKDLNCFEGVAGPTELQYFS
CEECCCCEEECCCHHHHHHHHHHHHHHHCCCCHHHHHEECCCCCCHHHCCCCCHHHHEEE
AFVIPVFGENKVLWTVRNITEYKTALQKLERQQLELEKLSCLDHLTQLYNRYALEVHLPS
EEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCH
AIANTAQSGKSAALFMVDIDCFKDLNDTYGHLTGDKALRMVSLALKHWSGHNGICFRYGG
HHHHHHCCCCCEEEEEEEHHHHHCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECC
DEFLVFIQDISQEECQSMAHSLQQSIQKLAIPNQHSHVADTLTLTIGIQFYPSLTGDSQD
CEEEEEEHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEEEEEEECCCCCCHHH
LEQFISKADKALFNAKNTQRGTVHFLSH
HHHHHHHHHHHHHCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]