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The map label for this gene is fixJ [H]

Identifier: 37680408

GI number: 37680408

Start: 2244623

End: 2245222

Strand: Direct

Name: fixJ [H]

Synonym: VV2224

Alternate gene names: 37680408

Gene position: 2244623-2245222 (Clockwise)

Preceding gene: 37680407

Following gene: 37680409

Centisome position: 66.91

GC content: 49.17

Gene sequence:

>600_bases
ATGAGTGGTAAACAATTACCCGTCTATGTGGTGGATGATGATGAATCAGTGCGAGATTCGCTGGCATTTATGCTCGAGGA
GTATGACTTTTTAGTGACCACCTTTGCCGATGGGCAAGCATTTCTCGACGGCGTAGATACTGAACAAGCAGGCTGCGTGA
TCCTCGATAGCAGAATGCCTAACCTGCGCGGACAACAAGTGCACCAGATTTTGAATGAGACCAAAAGCCCACTGGCGGTG
ATCTACCTCACCGGACACGGCGATGTGCCAATGGCGGTTGACGCCATGCAAGCGGGCGCGGTCAACTTTTTCCAAAAACC
GGTAAAAGGTGCTGAACTGGCGCAAGCAATAATTCAAGGGCAAGTGCAGTCCGCTGCGAACTTAGAGATTGCGCTAGCCA
AAAGAGCCTACCAATCACTCACCAGCAGAGAGAAAGATATTCTGCGCCTTATCATTGTAGGGAAACGTAATATTCGTATT
GCCGACGAACTTTGCATTGCAATGCGTACCGTAGAAGTGCACCGAGCCAACTTACTCAAGAAATTCGACGCCAAAACCGT
CGCAGAGCTAGCCTATACTTATGGAAAAATTGTCCGCTGA

Upstream 100 bases:

>100_bases
CTCTGTTTGCCCTACCAAGCAGTGCCATCATTGAGCAAAAACGCAATGAGCACTCCAAACGCAGCCACAGACAGCACAAA
CAGCACAAAAGGAACGAGAA

Downstream 100 bases:

>100_bases
TTTCAATCTTGTACCATTTATCCGTAAACTATAGGAAATCGACTTCTTTCGACCTACTGAATATCATTATGTTCTTTGTC
GTACTCTCCTATCAACGAGC

Product: putative tetrathionate reductase complex, response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MSGKQLPVYVVDDDESVRDSLAFMLEEYDFLVTTFADGQAFLDGVDTEQAGCVILDSRMPNLRGQQVHQILNETKSPLAV
IYLTGHGDVPMAVDAMQAGAVNFFQKPVKGAELAQAIIQGQVQSAANLEIALAKRAYQSLTSREKDILRLIIVGKRNIRI
ADELCIAMRTVEVHRANLLKKFDAKTVAELAYTYGKIVR

Sequences:

>Translated_199_residues
MSGKQLPVYVVDDDESVRDSLAFMLEEYDFLVTTFADGQAFLDGVDTEQAGCVILDSRMPNLRGQQVHQILNETKSPLAV
IYLTGHGDVPMAVDAMQAGAVNFFQKPVKGAELAQAIIQGQVQSAANLEIALAKRAYQSLTSREKDILRLIIVGKRNIRI
ADELCIAMRTVEVHRANLLKKFDAKTVAELAYTYGKIVR
>Mature_198_residues
SGKQLPVYVVDDDESVRDSLAFMLEEYDFLVTTFADGQAFLDGVDTEQAGCVILDSRMPNLRGQQVHQILNETKSPLAVI
YLTGHGDVPMAVDAMQAGAVNFFQKPVKGAELAQAIIQGQVQSAANLEIALAKRAYQSLTSREKDILRLIIVGKRNIRIA
DELCIAMRTVEVHRANLLKKFDAKTVAELAYTYGKIVR

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790102, Length=189, Percent_Identity=30.6878306878307, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788905, Length=151, Percent_Identity=33.112582781457, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1788222, Length=199, Percent_Identity=25.6281407035176, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1790299, Length=100, Percent_Identity=34, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 21972; Mature: 21840

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGKQLPVYVVDDDESVRDSLAFMLEEYDFLVTTFADGQAFLDGVDTEQAGCVILDSRMP
CCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEECCCHHHHCCCCCCCCCEEEEECCCC
NLRGQQVHQILNETKSPLAVIYLTGHGDVPMAVDAMQAGAVNFFQKPVKGAELAQAIIQG
CCCHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QVQSAANLEIALAKRAYQSLTSREKDILRLIIVGKRNIRIADELCIAMRTVEVHRANLLK
HHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
KFDAKTVAELAYTYGKIVR
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SGKQLPVYVVDDDESVRDSLAFMLEEYDFLVTTFADGQAFLDGVDTEQAGCVILDSRMP
CCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEECCCHHHHCCCCCCCCCEEEEECCCC
NLRGQQVHQILNETKSPLAVIYLTGHGDVPMAVDAMQAGAVNFFQKPVKGAELAQAIIQG
CCCHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QVQSAANLEIALAKRAYQSLTSREKDILRLIIVGKRNIRIADELCIAMRTVEVHRANLLK
HHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
KFDAKTVAELAYTYGKIVR
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]