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The map label for this gene is metR [H]

Identifier: 37680320

GI number: 37680320

Start: 2149835

End: 2150746

Strand: Direct

Name: metR [H]

Synonym: VV2136

Alternate gene names: 37680320

Gene position: 2149835-2150746 (Clockwise)

Preceding gene: 37680318

Following gene: 37680327

Centisome position: 64.09

GC content: 48.68

Gene sequence:

>912_bases
ATGATTGAGCTAAAACATTTAAAAACACTAACGTCATTGCGAGACACTGGCTCATTGACCGCTACGGCAACCTCACTGCA
TCTCACTCAATCGGCGCTCTCCCATCAAATTAAAGATTTGGAAGCGCGCATTGGTGGCCAGCTCTTTTTACGTAAAACTC
GCCCCGTCAAATTCACTTCAGAAGGGGAGATTTTGCTGCGGTTAGCCGACGAGATTTTGCCAAAGTTAGCCAGAGCAGAG
AATGAGTTGGCGAGCTTAAAAGAAGATGTGAATGGCCGCTTACACATGGCGATTGAATGCCACTCCTGCTTTCAGTGGCT
CATGCCTGCTCTAAAGGAATATCAGTTAACCTGGCCGAGCGTGACGCTCGATTTTTCCTCCGGTTTTGGCTTTGAGCCTC
TACCCGCTTTGATGGCTGGTGAACTGGATTTGGTGATCACGTCTGATATTCAGCCTCGCTCTGAAGTGCATTACGAACCA
CTGTTTGATTTTGAAATGCGCCTGATTACCGCCACTAATCATCCTCTAGCGAACAAAGAGCGCTTGCAACCTGACGATCT
CGCTAATCAAACCATGTTGACTTATCCGGTACAAAAACAACGCTTAGATGTGGTGAAACACTTTTTGGCGCCCGCTGGTG
TCGAGCCTGCTCGCTGGAAACAAGCAGACAACACACTGATGCTAGTGCAGATGGTTTCGGCTGGTTTGGGGGTTGCGGCT
TTGCCCAACTGGGCGATCAGTGAATTTTCTCGCCAAGGGCTGATCACCAGTATTCCATTAGGTAAAGGATTGTGGCGACG
ACTGTTTGCCGCCACACGCAATGCCGATAAAGACAAACGCTATCTTCAAGCCTTCTTTGCGACGGCGAGACAGCAATGTA
AAAGCCATCTTGACGGCATCAAGATGGCTTAA

Upstream 100 bases:

>100_bases
ACATCAACCAAAATAAATAGACGTCTAGATGTTTACAGCTCCAAAAAAACTGGGTAAGTTAAAAAGGCTCATGTAACGAG
CGATTGGTTGAGAGGGATTC

Downstream 100 bases:

>100_bases
GGGAATGGTCACTAGCGAGAGTATGATTTGAATTGGTTGGTGAGCGGATCGTATTGATAACCCAATACATCCAGTTTGCC
GACCACTTGCTCAACGTCCA

Product: transcriptional activator MetR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MIELKHLKTLTSLRDTGSLTATATSLHLTQSALSHQIKDLEARIGGQLFLRKTRPVKFTSEGEILLRLADEILPKLARAE
NELASLKEDVNGRLHMAIECHSCFQWLMPALKEYQLTWPSVTLDFSSGFGFEPLPALMAGELDLVITSDIQPRSEVHYEP
LFDFEMRLITATNHPLANKERLQPDDLANQTMLTYPVQKQRLDVVKHFLAPAGVEPARWKQADNTLMLVQMVSAGLGVAA
LPNWAISEFSRQGLITSIPLGKGLWRRLFAATRNADKDKRYLQAFFATARQQCKSHLDGIKMA

Sequences:

>Translated_303_residues
MIELKHLKTLTSLRDTGSLTATATSLHLTQSALSHQIKDLEARIGGQLFLRKTRPVKFTSEGEILLRLADEILPKLARAE
NELASLKEDVNGRLHMAIECHSCFQWLMPALKEYQLTWPSVTLDFSSGFGFEPLPALMAGELDLVITSDIQPRSEVHYEP
LFDFEMRLITATNHPLANKERLQPDDLANQTMLTYPVQKQRLDVVKHFLAPAGVEPARWKQADNTLMLVQMVSAGLGVAA
LPNWAISEFSRQGLITSIPLGKGLWRRLFAATRNADKDKRYLQAFFATARQQCKSHLDGIKMA
>Mature_303_residues
MIELKHLKTLTSLRDTGSLTATATSLHLTQSALSHQIKDLEARIGGQLFLRKTRPVKFTSEGEILLRLADEILPKLARAE
NELASLKEDVNGRLHMAIECHSCFQWLMPALKEYQLTWPSVTLDFSSGFGFEPLPALMAGELDLVITSDIQPRSEVHYEP
LFDFEMRLITATNHPLANKERLQPDDLANQTMLTYPVQKQRLDVVKHFLAPAGVEPARWKQADNTLMLVQMVSAGLGVAA
LPNWAISEFSRQGLITSIPLGKGLWRRLFAATRNADKDKRYLQAFFATARQQCKSHLDGIKMA

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=303, Percent_Identity=54.7854785478548, Blast_Score=353, Evalue=6e-99,
Organism=Escherichia coli, GI157672245, Length=228, Percent_Identity=24.1228070175439, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790399, Length=303, Percent_Identity=27.7227722772277, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788887, Length=247, Percent_Identity=28.7449392712551, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI145693105, Length=274, Percent_Identity=24.4525547445255, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1786401, Length=263, Percent_Identity=26.2357414448669, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790208, Length=245, Percent_Identity=25.3061224489796, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693193, Length=267, Percent_Identity=26.9662921348315, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787879, Length=249, Percent_Identity=25.7028112449799, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787530, Length=244, Percent_Identity=22.9508196721311, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082024, Length=179, Percent_Identity=29.0502793296089, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788297, Length=248, Percent_Identity=22.5806451612903, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789506, Length=181, Percent_Identity=24.3093922651934, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787601, Length=177, Percent_Identity=24.8587570621469, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87081904, Length=178, Percent_Identity=28.6516853932584, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34031; Mature: 34031

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50931 HTH_LYSR ; PS00300 SRP54

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELKHLKTLTSLRDTGSLTATATSLHLTQSALSHQIKDLEARIGGQLFLRKTRPVKFTS
CCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECC
EGEILLRLADEILPKLARAENELASLKEDVNGRLHMAIECHSCFQWLMPALKEYQLTWPS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHEEECCE
VTLDFSSGFGFEPLPALMAGELDLVITSDIQPRSEVHYEPLFDFEMRLITATNHPLANKE
EEEECCCCCCCCCHHHHHCCCEEEEEECCCCCCHHCCCCCCCCCEEEEEEECCCCCCCCC
RLQPDDLANQTMLTYPVQKQRLDVVKHFLAPAGVEPARWKQADNTLMLVQMVSAGLGVAA
CCCCHHHCCCEEEECCHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHCCCCCHH
LPNWAISEFSRQGLITSIPLGKGLWRRLFAATRNADKDKRYLQAFFATARQQCKSHLDGI
CCCHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
KMA
CCC
>Mature Secondary Structure
MIELKHLKTLTSLRDTGSLTATATSLHLTQSALSHQIKDLEARIGGQLFLRKTRPVKFTS
CCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECC
EGEILLRLADEILPKLARAENELASLKEDVNGRLHMAIECHSCFQWLMPALKEYQLTWPS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHEEECCE
VTLDFSSGFGFEPLPALMAGELDLVITSDIQPRSEVHYEPLFDFEMRLITATNHPLANKE
EEEECCCCCCCCCHHHHHCCCEEEEEECCCCCCHHCCCCCCCCCEEEEEEECCCCCCCCC
RLQPDDLANQTMLTYPVQKQRLDVVKHFLAPAGVEPARWKQADNTLMLVQMVSAGLGVAA
CCCCHHHCCCEEEECCHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHCCCCCHH
LPNWAISEFSRQGLITSIPLGKGLWRRLFAATRNADKDKRYLQAFFATARQQCKSHLDGI
CCCHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
KMA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]