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The map label for this gene is pyk [H]

Identifier: 37521568

GI number: 37521568

Start: 2146478

End: 2147983

Strand: Reverse

Name: pyk [H]

Synonym: gll1999

Alternate gene names: 37521568

Gene position: 2147983-2146478 (Counterclockwise)

Preceding gene: 37521570

Following gene: 37521567

Centisome position: 46.1

GC content: 64.14

Gene sequence:

>1506_bases
ATGCAAAGTAGCGACCCGGCCATTCGGTTTAGAGCGATGTCCGCCGTTCAACCGCAGCTCGAAGCACTGATCGATCAGCT
CGACACGATCCGCTCCCACGCAGAGAGCCTCAAGCAACAATTCGCTGAGCACCTTCAGCACGTTCATCCCAACTTTGCCG
ACAGTGCCCACAATCTCATCGACTACCTCGCCCTGCGCCAGTTCGACCTGCGCGAAATTCAGGAGCAGTTGAGTGGTCTG
GGCCTCTCGTCGCTGGGCCGCACCGAGGCGCACACCATGGCAAGTTTGAACGCCGTGGTGCAGGTGCTTGAGCGACTCGC
GGGCAGCGGCGGCGAGGTCGTTTCTTTTGCGGACGATTATGCCCGGGGCCGGGATCTGCTCACCGCCCACACCGAGGTGC
TCCTGGGACCGAGGCCCGCGACGCGCGCCACCCGGATCATGGTCACCCTACCCAGCGAAGCCGAAGGCGATTACCGGCTG
TTGTGCGCTCTCATCCGGGCCGGGATGAATTGTGCGCGCATCAACTGCGTCCACGACAGCGAGACGGTCTGGGAGCGCAT
GGTGGGCAACATCCGCCGCGCCGAGCGGGAGGTGGGGCGCGCCTGCCGGATCTTGATGGACCTGGGCGGACCGAAATTGC
GCACCGGACCGCTTGCCGAACCGCTCACCCTCCGCAAAGGTGAGGGGCTCGTCCTCTGCCGCGACGCAGAAGAGGGCCGA
TCTGCCTGCGAAGATTCCCCGGCGCGCGTGGTGTGCGCGGTAAGCGGGATCTACGGCGGTGTGCAGGTGGGCGAGGCGGT
GCTGTTCGACGACGGCAAAATCGAGAGCGTCGTGCGCGGGGTAGCGCAGGACGAAATCCAGCTGGAGATCACCCGGGCCG
ACGACAAAGGCAGTCGCCTGGCCGCCGACAAGGGGATCAATTTTCCCGAGAGCCGCCTGAAGCTTCGGGGGCTGAGCGAA
CAGGATCTGGAGCACCTCGATTTTGTCGCCCGCCGCGCCGACATCGTCGGAATGTCCTTCGCCAATGAACCCGAGGATGT
CTTTGCGCTGCAGGCGGCCCTGGGCGAGCGGGGGGCAGGACATTTGGGCATTTTGCTCAAGATCGAAACGCGCCGGGGCT
TCGAGCAGTTACCAAGGCTGATCCTGGCTGCCATGCGCTCCTATCCGGCCGGGGTGATGATTGCCCGCGGGGATCTGGCG
GTCGAGTGCGGCTGGGAGCGCACCGCCGAGGTGCAGGAGGAAATCCTCTGGCTGTGTGAAGCGGGGCACATGCCGGTGGT
CTGGGCCACCCAGGTGCTCGAAAAACTGGCCAAAAAGGGTTTGCCCTCGCGCGCCGAGATTACCGACGCGGCGATGAGCC
AGCGCGCCGAGTGCGTCATGCTCAACAAAGGCCCCCACATCGTGGAAGCTGTGCACTCCCTCGCCGACATCCTCTGCCGC
ATGCAGGAGCACCAGCACAAAAAAACCGCCACCCTGCGCAGCTTAAAAGTTTCCAACCTTGTCTAG

Upstream 100 bases:

>100_bases
TGCCTTCTGCTTGATTGGACGCCGCGCGCCCCCGGTGGGTTTCGGTCGTCTGTAACAGACTGCCATCGGATACAGGTGGT
GGGCAACCCCTGCCGCTATC

Downstream 100 bases:

>100_bases
AGTGCATGTGCCAAACGCCGCTGCCAAGCATCGGTATATTTGAGGGAGCACTAAGCCCCGAATGCGCGTGTACCCGCTCT
ACGAAACGGATTTCTACGCC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MQSSDPAIRFRAMSAVQPQLEALIDQLDTIRSHAESLKQQFAEHLQHVHPNFADSAHNLIDYLALRQFDLREIQEQLSGL
GLSSLGRTEAHTMASLNAVVQVLERLAGSGGEVVSFADDYARGRDLLTAHTEVLLGPRPATRATRIMVTLPSEAEGDYRL
LCALIRAGMNCARINCVHDSETVWERMVGNIRRAEREVGRACRILMDLGGPKLRTGPLAEPLTLRKGEGLVLCRDAEEGR
SACEDSPARVVCAVSGIYGGVQVGEAVLFDDGKIESVVRGVAQDEIQLEITRADDKGSRLAADKGINFPESRLKLRGLSE
QDLEHLDFVARRADIVGMSFANEPEDVFALQAALGERGAGHLGILLKIETRRGFEQLPRLILAAMRSYPAGVMIARGDLA
VECGWERTAEVQEEILWLCEAGHMPVVWATQVLEKLAKKGLPSRAEITDAAMSQRAECVMLNKGPHIVEAVHSLADILCR
MQEHQHKKTATLRSLKVSNLV

Sequences:

>Translated_501_residues
MQSSDPAIRFRAMSAVQPQLEALIDQLDTIRSHAESLKQQFAEHLQHVHPNFADSAHNLIDYLALRQFDLREIQEQLSGL
GLSSLGRTEAHTMASLNAVVQVLERLAGSGGEVVSFADDYARGRDLLTAHTEVLLGPRPATRATRIMVTLPSEAEGDYRL
LCALIRAGMNCARINCVHDSETVWERMVGNIRRAEREVGRACRILMDLGGPKLRTGPLAEPLTLRKGEGLVLCRDAEEGR
SACEDSPARVVCAVSGIYGGVQVGEAVLFDDGKIESVVRGVAQDEIQLEITRADDKGSRLAADKGINFPESRLKLRGLSE
QDLEHLDFVARRADIVGMSFANEPEDVFALQAALGERGAGHLGILLKIETRRGFEQLPRLILAAMRSYPAGVMIARGDLA
VECGWERTAEVQEEILWLCEAGHMPVVWATQVLEKLAKKGLPSRAEITDAAMSQRAECVMLNKGPHIVEAVHSLADILCR
MQEHQHKKTATLRSLKVSNLV
>Mature_501_residues
MQSSDPAIRFRAMSAVQPQLEALIDQLDTIRSHAESLKQQFAEHLQHVHPNFADSAHNLIDYLALRQFDLREIQEQLSGL
GLSSLGRTEAHTMASLNAVVQVLERLAGSGGEVVSFADDYARGRDLLTAHTEVLLGPRPATRATRIMVTLPSEAEGDYRL
LCALIRAGMNCARINCVHDSETVWERMVGNIRRAEREVGRACRILMDLGGPKLRTGPLAEPLTLRKGEGLVLCRDAEEGR
SACEDSPARVVCAVSGIYGGVQVGEAVLFDDGKIESVVRGVAQDEIQLEITRADDKGSRLAADKGINFPESRLKLRGLSE
QDLEHLDFVARRADIVGMSFANEPEDVFALQAALGERGAGHLGILLKIETRRGFEQLPRLILAAMRSYPAGVMIARGDLA
VECGWERTAEVQEEILWLCEAGHMPVVWATQVLEKLAKKGLPSRAEITDAAMSQRAECVMLNKGPHIVEAVHSLADILCR
MQEHQHKKTATLRSLKVSNLV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=357, Percent_Identity=33.0532212885154, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI32967597, Length=357, Percent_Identity=33.0532212885154, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI33286422, Length=356, Percent_Identity=30.3370786516854, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI33286420, Length=356, Percent_Identity=30.3370786516854, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI33286418, Length=356, Percent_Identity=30.3370786516854, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI310128732, Length=309, Percent_Identity=29.126213592233, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI310128730, Length=309, Percent_Identity=29.126213592233, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI310128736, Length=252, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI310128734, Length=252, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI310128738, Length=225, Percent_Identity=28.8888888888889, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1787965, Length=348, Percent_Identity=28.735632183908, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788160, Length=347, Percent_Identity=29.3948126801153, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17544584, Length=357, Percent_Identity=29.9719887955182, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71984413, Length=355, Percent_Identity=30.9859154929577, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71984406, Length=355, Percent_Identity=30.9859154929577, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17506829, Length=355, Percent_Identity=30.9859154929577, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17506831, Length=355, Percent_Identity=30.9859154929577, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319279, Length=326, Percent_Identity=30.3680981595092, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324923, Length=325, Percent_Identity=28.9230769230769, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI28571814, Length=372, Percent_Identity=31.7204301075269, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24648964, Length=372, Percent_Identity=31.7204301075269, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24648966, Length=291, Percent_Identity=29.2096219931272, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24581235, Length=336, Percent_Identity=26.1904761904762, Blast_Score=99, Evalue=8e-21,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 55000; Mature: 55000

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSSDPAIRFRAMSAVQPQLEALIDQLDTIRSHAESLKQQFAEHLQHVHPNFADSAHNLI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
DYLALRQFDLREIQEQLSGLGLSSLGRTEAHTMASLNAVVQVLERLAGSGGEVVSFADDY
HHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHH
ARGRDLLTAHTEVLLGPRPATRATRIMVTLPSEAEGDYRLLCALIRAGMNCARINCVHDS
HCCCHHHHHHHHHEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEECCH
ETVWERMVGNIRRAEREVGRACRILMDLGGPKLRTGPLAEPLTLRKGEGLVLCRDAEEGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCEEEEECCHHHH
SACEDSPARVVCAVSGIYGGVQVGEAVLFDDGKIESVVRGVAQDEIQLEITRADDKGSRL
HHCCCCCCEEEEEEHHHCCCHHHCCEEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCEE
AADKGINFPESRLKLRGLSEQDLEHLDFVARRADIVGMSFANEPEDVFALQAALGERGAG
ECCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCCC
HLGILLKIETRRGFEQLPRLILAAMRSYPAGVMIARGDLAVECGWERTAEVQEEILWLCE
CEEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHC
AGHMPVVWATQVLEKLAKKGLPSRAEITDAAMSQRAECVMLNKGPHIVEAVHSLADILCR
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHH
MQEHQHKKTATLRSLKVSNLV
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQSSDPAIRFRAMSAVQPQLEALIDQLDTIRSHAESLKQQFAEHLQHVHPNFADSAHNLI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
DYLALRQFDLREIQEQLSGLGLSSLGRTEAHTMASLNAVVQVLERLAGSGGEVVSFADDY
HHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHH
ARGRDLLTAHTEVLLGPRPATRATRIMVTLPSEAEGDYRLLCALIRAGMNCARINCVHDS
HCCCHHHHHHHHHEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEECCH
ETVWERMVGNIRRAEREVGRACRILMDLGGPKLRTGPLAEPLTLRKGEGLVLCRDAEEGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCEEEEECCHHHH
SACEDSPARVVCAVSGIYGGVQVGEAVLFDDGKIESVVRGVAQDEIQLEITRADDKGSRL
HHCCCCCCEEEEEEHHHCCCHHHCCEEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCEE
AADKGINFPESRLKLRGLSEQDLEHLDFVARRADIVGMSFANEPEDVFALQAALGERGAG
ECCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCCC
HLGILLKIETRRGFEQLPRLILAAMRSYPAGVMIARGDLAVECGWERTAEVQEEILWLCE
CEEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHC
AGHMPVVWATQVLEKLAKKGLPSRAEITDAAMSQRAECVMLNKGPHIVEAVHSLADILCR
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHH
MQEHQHKKTATLRSLKVSNLV
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7549104 [H]