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The map label for this gene is rsmA

Identifier: 37521560

GI number: 37521560

Start: 2139916

End: 2140734

Strand: Reverse

Name: rsmA

Synonym: gll1991

Alternate gene names: 37521560

Gene position: 2140734-2139916 (Counterclockwise)

Preceding gene: 37521567

Following gene: 37521558

Centisome position: 45.95

GC content: 63.86

Gene sequence:

>819_bases
ATGTACACTCTCAAACGTTTTGGTCAGCACTGGCTCAACGACGGGGCGGTTCTCGATCGCATCGTGGCCGCCGCCGGACT
CGCCTGTGGCGATCGGGTGCTGGAGATTGGTCCGGGTCTGGGCAGTCTCACCGCTCGGCTGTTGCGCCAGGTCCCGGTGG
TGGCCGTCGAAATCGACCGGCGGGCGGTGGCCCAGTTGCAGCGGCAGTTTGGCGGTGATAAGCGCTTCGTGCTGGTGGAG
GGAGACATCCTGCGAGAAGCGCTGCCCGAGCCCGCGAACGTCGTGGTGGCCAACATTCCCTACAACATCAGCGGCCCGAT
TCTGGCGAAGCTTACCGGCAGTCTGGCACAGCCCATTCGGCGCTTCCGCACCATCGTGTTGCTGGTACAAAAGGAACTGG
GCCAACGCATCGCCGCCCCGCCCGGCAGCCGCACCTACGGCGCGCTCTCGGTGCGATTGCAGTACCTGGCCGAGTGCGAA
CTGCTCTTTGAAGTCCCGTCCCACTGCTTCACCCCACCGCCCAAGGTCGATTCGGCGGTGATTCGCCTCACGCCGCGCCC
TTTTGCCCTGCAGGCCGACGATCCGGCCCATCTCGACGCGCTGGTCACCCGCGCCTTCGCCACCCGCCGCAAGATGCTCA
AAAATTGTCTCAAAGGCTGGGTCGAAACGGAAAAGCTCCTCGCTGCTTTTGCCTCGCTAGATATCAGTCCGGATGCCCGC
GCTGAGGATTTGAGCGTTGAGCGCTTCGTGCAGCTGAGCAACCGTTTGGCAGAGAGTGACTATCCGTCGGGTGCGGCGCG
TGGCGACCGACGTGCATAA

Upstream 100 bases:

>100_bases
GGCAAGGGCGCAAAGGTTGCAGATTAGTGTAGCACGGGATATTGCGCCTACCGGGCCGCAGCCTGTGCTTCGCGGCCATT
CCGGATCGATTTGATTTGAT

Downstream 100 bases:

>100_bases
CCATAGAGCACAAAACTATTCTCCTCTGTAACTGCAACGCTCACCGCATTCTTGGTTCTTGGAGAAACACCTATGCCCGA
ACCAACGGTAGAAGAAACGC

Product: rRNA (adenine-N6,N6)-dimethyltransferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MYTLKRFGQHWLNDGAVLDRIVAAAGLACGDRVLEIGPGLGSLTARLLRQVPVVAVEIDRRAVAQLQRQFGGDKRFVLVE
GDILREALPEPANVVVANIPYNISGPILAKLTGSLAQPIRRFRTIVLLVQKELGQRIAAPPGSRTYGALSVRLQYLAECE
LLFEVPSHCFTPPPKVDSAVIRLTPRPFALQADDPAHLDALVTRAFATRRKMLKNCLKGWVETEKLLAAFASLDISPDAR
AEDLSVERFVQLSNRLAESDYPSGAARGDRRA

Sequences:

>Translated_272_residues
MYTLKRFGQHWLNDGAVLDRIVAAAGLACGDRVLEIGPGLGSLTARLLRQVPVVAVEIDRRAVAQLQRQFGGDKRFVLVE
GDILREALPEPANVVVANIPYNISGPILAKLTGSLAQPIRRFRTIVLLVQKELGQRIAAPPGSRTYGALSVRLQYLAECE
LLFEVPSHCFTPPPKVDSAVIRLTPRPFALQADDPAHLDALVTRAFATRRKMLKNCLKGWVETEKLLAAFASLDISPDAR
AEDLSVERFVQLSNRLAESDYPSGAARGDRRA
>Mature_272_residues
MYTLKRFGQHWLNDGAVLDRIVAAAGLACGDRVLEIGPGLGSLTARLLRQVPVVAVEIDRRAVAQLQRQFGGDKRFVLVE
GDILREALPEPANVVVANIPYNISGPILAKLTGSLAQPIRRFRTIVLLVQKELGQRIAAPPGSRTYGALSVRLQYLAECE
LLFEVPSHCFTPPPKVDSAVIRLTPRPFALQADDPAHLDALVTRAFATRRKMLKNCLKGWVETEKLLAAFASLDISPDAR
AEDLSVERFVQLSNRLAESDYPSGAARGDRRA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=220, Percent_Identity=36.8181818181818, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI156415992, Length=282, Percent_Identity=29.7872340425532, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786236, Length=269, Percent_Identity=38.2899628252788, Blast_Score=148, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI25146882, Length=183, Percent_Identity=40.9836065573771, Blast_Score=130, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI25141369, Length=291, Percent_Identity=27.4914089347079, Blast_Score=94, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6324989, Length=188, Percent_Identity=38.8297872340425, Blast_Score=115, Evalue=8e-27,
Organism=Drosophila melanogaster, GI21358017, Length=186, Percent_Identity=40.8602150537634, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI21357273, Length=284, Percent_Identity=30.6338028169014, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_GLOVI (Q7NJ41)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_924937.1
- ProteinModelPortal:   Q7NJ41
- SMR:   Q7NJ41
- GeneID:   2600393
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll1991
- NMPDR:   fig|251221.1.peg.1991
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- BioCyc:   GVIO251221:GLL1991-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 29810; Mature: 29810

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 10-10 BINDING 12-12 BINDING 37-37 BINDING 57-57 BINDING 82-82 BINDING 98-98

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTLKRFGQHWLNDGAVLDRIVAAAGLACGDRVLEIGPGLGSLTARLLRQVPVVAVEIDR
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHCCEEEEECCH
RAVAQLQRQFGGDKRFVLVEGDILREALPEPANVVVANIPYNISGPILAKLTGSLAQPIR
HHHHHHHHHHCCCCEEEEEECHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
RFRTIVLLVQKELGQRIAAPPGSRTYGALSVRLQYLAECELLFEVPSHCFTPPPKVDSAV
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
IRLTPRPFALQADDPAHLDALVTRAFATRRKMLKNCLKGWVETEKLLAAFASLDISPDAR
EEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AEDLSVERFVQLSNRLAESDYPSGAARGDRRA
CHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MYTLKRFGQHWLNDGAVLDRIVAAAGLACGDRVLEIGPGLGSLTARLLRQVPVVAVEIDR
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHCCEEEEECCH
RAVAQLQRQFGGDKRFVLVEGDILREALPEPANVVVANIPYNISGPILAKLTGSLAQPIR
HHHHHHHHHHCCCCEEEEEECHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
RFRTIVLLVQKELGQRIAAPPGSRTYGALSVRLQYLAECELLFEVPSHCFTPPPKVDSAV
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
IRLTPRPFALQADDPAHLDALVTRAFATRRKMLKNCLKGWVETEKLLAAFASLDISPDAR
EEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AEDLSVERFVQLSNRLAESDYPSGAARGDRRA
CHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292