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The map label for this gene is ppsB [H]

Identifier: 37521513

GI number: 37521513

Start: 2069371

End: 2071230

Strand: Reverse

Name: ppsB [H]

Synonym: gll1944

Alternate gene names: 37521513

Gene position: 2071230-2069371 (Counterclockwise)

Preceding gene: 37521514

Following gene: 37521512

Centisome position: 44.46

GC content: 68.06

Gene sequence:

>1860_bases
ATGGACACCGGCACCCGGATGGGGGAGCCCATCGCCATCGTCGGGATGGGCTGCCGGTTTCCCGGCGGGGCTGAAAACCC
CGAGCGCTTCTGGCAGCTGCTGCGCGACGGGGTCGACGCGATTCGCGATGTGCCCCCCGAACGCTGGAACCTGGCGGATC
TGTACGATCCCGCTCCCGGGGCGCCGGGAAAGACCTGCACGCGCCGGGGCGGTTTTCTGGAGCAGGTGGACCGGTTCGAC
GCCGAATTTTTTGGCATCTCCCCACCCGAGGCGCAGCGGATGGACCCCCAGCAGCGGCTGGTCCTGGAGGTGGCCTGGGA
AGCGCTTGAAGATGCCGCCATCGTGCCTGTGGCGCTCGCCGGCAGCCGGACGGGAGTGTTTATCGGCATCTGCCACAGCG
ACTACGACACGCTCGCCTTTCGCCAGTACTCGGGTATTCACATGCTGGACACCACCGGCACCTCCGACTGCATCGCCGCC
AACCGCCTGTCCTACCTGCTGGACCTGCGCGGCCCGAGTCTGGCAATCGACACGGCCTGTTCCTCCTCGCTGGCGGCTTT
GCACCTGGCCTGCCAGAGTCTGTGGTTGGCAGAGTCGACCCTGGCGCTGGCGGGCGGCGTGCACCTGAACCTGTCTCCGG
AGCGCAGCATCGGCCTCAGTCAGGGGCGGATGCTGGCGACGGACGGCCGCTGCAAGACCTTCGACGCGCAGGCCGACGGC
TACGTGCGCGGCGAGGGCTGCGGGGTGCTCGTTCTGAAGCGTCTGAGCGACGCGCTCGCCGATCGAGACCCGATCCGGGC
GATTATCCGGGGGACCGCCGTCAACCACAACGGCCGCAGCAACGGCCTGACCGCCCCGAACACCGCCGCGCAGGAGGAAG
TGATCCGCCGGGCTCTTGCCCAGGCAGGCGTACAACCGTCGCAGGTGAGCTATGTCGAGACCCACGCGACCGGCACCGCC
CTGGGGGATCTTATCGAGTTCAAGGCCCTCAAGGCCGTACTGGGTCAGCGTCAGCAAGGCGATCCACGTTGCTTGCTGGG
CTCGCTCAAGACGAACATCGGCCATCTCGAGGCCGCCGCCGGAATCGCCGGGCTCATCAAGGTCACCCTGGCCCTCCAAC
ACCGACAGATTCCACCTTCTTTATCCCCCCAGCGGCTGCATCCTTACATTCGCCTCGACGGCACCCCCTTTGAAATTCCG
ACCCGGGGACAATCCTGGGAGCCTGGGGGCGAATTGCGCCTGGCCGGGGTGAGCGCCTTCGGCTTCGGCGGCACCAACGC
CCACCTCCTCCTGCAAGAAGCCCCCCCCGAACCGCCTGCTCCCCCCAACCACCCGACTATCCCCCACCTGCTCACCCTCA
CCGCCCGCTCCGATGCCGCCCTGCGCCAACTGGCCGGGCGCTTCGCCAATCACCTGGACGCCGCCCACCCCGCCCACCTG
CCGGATATTTGCTTTTCGGCCAACACCGGACGCACACTGTTCGCCTTTCGGCTGGCGCTCGTGGCGGAGCACCCCGCCGA
GATGGCGGGCCTGCTGGCAGCTTTTGGCTGTGGCCAAAGACCGCCGGGAGTGCACTTTGGCCACAGCCCGCGGCGGCGGA
CGGCGGTGGTGGAGGTGGAGGAGGGTGCCTATCCGCGGCCGCAAAGCTCGTTGGCAGAGCGGCGGCGGATGCTGGAGGTG
CTGGGTGGGTGGTTCGTAGAAGGCTCGGCGGCGGTGGAGTGGGGGCGGTTGGCGGGGGATGGGCGGATGCGGCGGGTGTC
GCTGCCGACTTACCCCTTCGAGCGACAACGCTACTGGATGGAGTCGCCCCCCGCCGCGACGGCGGCAGTCAAAGCATCAG
ATCAGACGAAGGAGGTTTAA

Upstream 100 bases:

>100_bases
GCGATCTCGAAGCCTGGCTGGGCTGCGAACTTGATCCGGCCCTGCTGTACGACTACCAGACCATCGAGATCCTGGCCCGC
CACCTGGCTGGCCGGGTTTG

Downstream 100 bases:

>100_bases
AGGCATGGAAGTGCAGCATCTTGACCGTATCCGGCCTTCCGGCGATTGCCCGACGGCGCCGCAAATCGAAGCCTGGTTGG
CTGCCCGCCTGGCCCAGTTT

Product: polyketide synthase

Products: [acyl carrier protein]; acetoacetyl-ACP; CO2; [acyl carrier protein]; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MDTGTRMGEPIAIVGMGCRFPGGAENPERFWQLLRDGVDAIRDVPPERWNLADLYDPAPGAPGKTCTRRGGFLEQVDRFD
AEFFGISPPEAQRMDPQQRLVLEVAWEALEDAAIVPVALAGSRTGVFIGICHSDYDTLAFRQYSGIHMLDTTGTSDCIAA
NRLSYLLDLRGPSLAIDTACSSSLAALHLACQSLWLAESTLALAGGVHLNLSPERSIGLSQGRMLATDGRCKTFDAQADG
YVRGEGCGVLVLKRLSDALADRDPIRAIIRGTAVNHNGRSNGLTAPNTAAQEEVIRRALAQAGVQPSQVSYVETHATGTA
LGDLIEFKALKAVLGQRQQGDPRCLLGSLKTNIGHLEAAAGIAGLIKVTLALQHRQIPPSLSPQRLHPYIRLDGTPFEIP
TRGQSWEPGGELRLAGVSAFGFGGTNAHLLLQEAPPEPPAPPNHPTIPHLLTLTARSDAALRQLAGRFANHLDAAHPAHL
PDICFSANTGRTLFAFRLALVAEHPAEMAGLLAAFGCGQRPPGVHFGHSPRRRTAVVEVEEGAYPRPQSSLAERRRMLEV
LGGWFVEGSAAVEWGRLAGDGRMRRVSLPTYPFERQRYWMESPPAATAAVKASDQTKEV

Sequences:

>Translated_619_residues
MDTGTRMGEPIAIVGMGCRFPGGAENPERFWQLLRDGVDAIRDVPPERWNLADLYDPAPGAPGKTCTRRGGFLEQVDRFD
AEFFGISPPEAQRMDPQQRLVLEVAWEALEDAAIVPVALAGSRTGVFIGICHSDYDTLAFRQYSGIHMLDTTGTSDCIAA
NRLSYLLDLRGPSLAIDTACSSSLAALHLACQSLWLAESTLALAGGVHLNLSPERSIGLSQGRMLATDGRCKTFDAQADG
YVRGEGCGVLVLKRLSDALADRDPIRAIIRGTAVNHNGRSNGLTAPNTAAQEEVIRRALAQAGVQPSQVSYVETHATGTA
LGDLIEFKALKAVLGQRQQGDPRCLLGSLKTNIGHLEAAAGIAGLIKVTLALQHRQIPPSLSPQRLHPYIRLDGTPFEIP
TRGQSWEPGGELRLAGVSAFGFGGTNAHLLLQEAPPEPPAPPNHPTIPHLLTLTARSDAALRQLAGRFANHLDAAHPAHL
PDICFSANTGRTLFAFRLALVAEHPAEMAGLLAAFGCGQRPPGVHFGHSPRRRTAVVEVEEGAYPRPQSSLAERRRMLEV
LGGWFVEGSAAVEWGRLAGDGRMRRVSLPTYPFERQRYWMESPPAATAAVKASDQTKEV
>Mature_619_residues
MDTGTRMGEPIAIVGMGCRFPGGAENPERFWQLLRDGVDAIRDVPPERWNLADLYDPAPGAPGKTCTRRGGFLEQVDRFD
AEFFGISPPEAQRMDPQQRLVLEVAWEALEDAAIVPVALAGSRTGVFIGICHSDYDTLAFRQYSGIHMLDTTGTSDCIAA
NRLSYLLDLRGPSLAIDTACSSSLAALHLACQSLWLAESTLALAGGVHLNLSPERSIGLSQGRMLATDGRCKTFDAQADG
YVRGEGCGVLVLKRLSDALADRDPIRAIIRGTAVNHNGRSNGLTAPNTAAQEEVIRRALAQAGVQPSQVSYVETHATGTA
LGDLIEFKALKAVLGQRQQGDPRCLLGSLKTNIGHLEAAAGIAGLIKVTLALQHRQIPPSLSPQRLHPYIRLDGTPFEIP
TRGQSWEPGGELRLAGVSAFGFGGTNAHLLLQEAPPEPPAPPNHPTIPHLLTLTARSDAALRQLAGRFANHLDAAHPAHL
PDICFSANTGRTLFAFRLALVAEHPAEMAGLLAAFGCGQRPPGVHFGHSPRRRTAVVEVEEGAYPRPQSSLAERRRMLEV
LGGWFVEGSAAVEWGRLAGDGRMRRVSLPTYPFERQRYWMESPPAATAAVKASDQTKEV

Specific function: Polyketide synthase involved in phenolpthiocerol synthesis [H]

COG id: COG3321

COG function: function code Q; Polyketide synthase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Homo sapiens, GI41872631, Length=484, Percent_Identity=37.8099173553719, Blast_Score=269, Evalue=6e-72,
Organism=Homo sapiens, GI8923559, Length=437, Percent_Identity=27.0022883295195, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI310129082, Length=106, Percent_Identity=42.4528301886792, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI310109937, Length=106, Percent_Identity=42.4528301886792, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI239744023, Length=106, Percent_Identity=42.4528301886792, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI223972627, Length=158, Percent_Identity=34.8101265822785, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1787337, Length=283, Percent_Identity=30.7420494699647, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1788663, Length=288, Percent_Identity=27.4305555555556, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI212642053, Length=493, Percent_Identity=33.6713995943205, Blast_Score=255, Evalue=6e-68,
Organism=Caenorhabditis elegans, GI17550940, Length=507, Percent_Identity=33.1360946745562, Blast_Score=234, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI71983541, Length=359, Percent_Identity=26.1838440111421, Blast_Score=105, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71983535, Length=270, Percent_Identity=28.1481481481481, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6320904, Length=448, Percent_Identity=26.1160714285714, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI221330659, Length=461, Percent_Identity=35.3579175704989, Blast_Score=248, Evalue=1e-65,
Organism=Drosophila melanogaster, GI19920632, Length=461, Percent_Identity=35.3579175704989, Blast_Score=248, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24581345, Length=462, Percent_Identity=34.1991341991342, Blast_Score=242, Evalue=7e-64,
Organism=Drosophila melanogaster, GI24644341, Length=364, Percent_Identity=29.3956043956044, Blast_Score=122, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001227
- InterPro:   IPR009081
- InterPro:   IPR014043
- InterPro:   IPR016035
- InterPro:   IPR000794
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016036
- InterPro:   IPR016040
- InterPro:   IPR006163
- InterPro:   IPR020801
- InterPro:   IPR020841
- InterPro:   IPR013968
- InterPro:   IPR020806
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00698 Acyl_transf_1; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF08659 KR; PF00550 PP-binding [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 66579; Mature: 66579

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTGTRMGEPIAIVGMGCRFPGGAENPERFWQLLRDGVDAIRDVPPERWNLADLYDPAPG
CCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCC
APGKTCTRRGGFLEQVDRFDAEFFGISPPEAQRMDPQQRLVLEVAWEALEDAAIVPVALA
CCCCCHHHCCCHHHHHHHHCHHHCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEE
GSRTGVFIGICHSDYDTLAFRQYSGIHMLDTTGTSDCIAANRLSYLLDLRGPSLAIDTAC
CCCCEEEEEEECCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHEECCCCCEEEEHHH
SSSLAALHLACQSLWLAESTLALAGGVHLNLSPERSIGLSQGRMLATDGRCKTFDAQADG
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEECCCCEEECCCCCC
YVRGEGCGVLVLKRLSDALADRDPIRAIIRGTAVNHNGRSNGLTAPNTAAQEEVIRRALA
EEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCEECCCCCCCCCCCCCCHHHHHHHHHHHH
QAGVQPSQVSYVETHATGTALGDLIEFKALKAVLGQRQQGDPRCLLGSLKTNIGHLEAAA
HCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHH
GIAGLIKVTLALQHRQIPPSLSPQRLHPYIRLDGTPFEIPTRGQSWEPGGELRLAGVSAF
HHHHHHHHHHHHHHCCCCCCCCHHHCCCEEEECCCCEECCCCCCCCCCCCCEEEEEEEEE
GFGGTNAHLLLQEAPPEPPAPPNHPTIPHLLTLTARSDAALRQLAGRFANHLDAAHPAHL
CCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCC
PDICFSANTGRTLFAFRLALVAEHPAEMAGLLAAFGCGQRPPGVHFGHSPRRRTAVVEVE
CHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEC
EGAYPRPQSSLAERRRMLEVLGGWFVEGSAAVEWGRLAGDGRMRRVSLPTYPFERQRYWM
CCCCCCCHHHHHHHHHHHHHHCCEEECCCCCEECCEECCCCCEEEEECCCCCCHHHHHCC
ESPPAATAAVKASDQTKEV
CCCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MDTGTRMGEPIAIVGMGCRFPGGAENPERFWQLLRDGVDAIRDVPPERWNLADLYDPAPG
CCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCC
APGKTCTRRGGFLEQVDRFDAEFFGISPPEAQRMDPQQRLVLEVAWEALEDAAIVPVALA
CCCCCHHHCCCHHHHHHHHCHHHCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEE
GSRTGVFIGICHSDYDTLAFRQYSGIHMLDTTGTSDCIAANRLSYLLDLRGPSLAIDTAC
CCCCEEEEEEECCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHEECCCCCEEEEHHH
SSSLAALHLACQSLWLAESTLALAGGVHLNLSPERSIGLSQGRMLATDGRCKTFDAQADG
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEECCCCEEECCCCCC
YVRGEGCGVLVLKRLSDALADRDPIRAIIRGTAVNHNGRSNGLTAPNTAAQEEVIRRALA
EEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCEECCCCCCCCCCCCCCHHHHHHHHHHHH
QAGVQPSQVSYVETHATGTALGDLIEFKALKAVLGQRQQGDPRCLLGSLKTNIGHLEAAA
HCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHH
GIAGLIKVTLALQHRQIPPSLSPQRLHPYIRLDGTPFEIPTRGQSWEPGGELRLAGVSAF
HHHHHHHHHHHHHHCCCCCCCCHHHCCCEEEECCCCEECCCCCCCCCCCCCEEEEEEEEE
GFGGTNAHLLLQEAPPEPPAPPNHPTIPHLLTLTARSDAALRQLAGRFANHLDAAHPAHL
CCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCC
PDICFSANTGRTLFAFRLALVAEHPAEMAGLLAAFGCGQRPPGVHFGHSPRRRTAVVEVE
CHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEC
EGAYPRPQSSLAERRRMLEVLGGWFVEGSAAVEWGRLAGDGRMRRVSLPTYPFERQRYWM
CCCCCCCHHHHHHHHHHHHHHCCEEECCCCCEECCEECCCCCEEEEECCCCCCHHHHHCC
ESPPAATAAVKASDQTKEV
CCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} [C]

Substrates: acetyl-ACP; malonyl-ACP; acyl-ACP [C]

Specific reaction: acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]