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The map label for this gene is folD

Identifier: 37521453

GI number: 37521453

Start: 2002847

End: 2003719

Strand: Reverse

Name: folD

Synonym: gll1884

Alternate gene names: 37521453

Gene position: 2003719-2002847 (Counterclockwise)

Preceding gene: 37521454

Following gene: 37521451

Centisome position: 43.01

GC content: 66.55

Gene sequence:

>873_bases
TTGTCCGCAGCCATTCTCGACGGCAAAGCCCTGGCCGCCCGCATTCAGGCCGAGCTGACTTGCGAGGTGCAGTCGCTGCA
GGAGCGCTTCGGCCGCCCGCCCGGCCTCTCCGTGCTGTGGGTGGGAGACAACCCGGCCTCCGCCGCCTACGTCCGCAACA
AAAACAAATCTGGTCAAGCGGTGGGGATCGATGTCGCCAAAAGTCAGCACCTCAAAGCGAGCCTCTCCGAGACGCAACTG
TTGGCCCTCATCGATCGCCTCAACGCCGATGTGACCGTGGACGGCATCCTCGTCCAACTACCGTTGCCGGAGCACATCGA
CAGCGGTCGGGTACTGAACCGCATCGCCCCCGAAAAAGACGTCGACGGTCTGCACCCGGTCAATCTGGGGCGGCTGGTGC
GCGGCGAGCCGGGCTTGCGCTCGTGCACCCCGGCCGGGGTGATGCGGATCCTCTCTGAGGCGGGGGTGCCCCTGGCGGGC
AAGACAGCCGTCGTCATCGGCCGCAGCATCCTGGTGGGCAAACCCGTCGCCCTGATGCTGCTCGAAAAAGACGCGACCGT
AATCACCACCCACTCGCGCACGGCGGATCTGGCCGCCATCACCCAGATGGCGGACGTGCTGGTGGTGGCGGCCGGGCGGC
CGGAGCTGGTCACCGCCGCCATGGTCCGGCCCGGGGCGGTGGTAATCGATGTGGGCATCAACCGCGTCACCGACTACGAG
GGCAACAGCCGCCTGGTGGGCGACGTCGATTTTGATTCTGTGCGCGACGTGGCGGCGGCGATCACCCCGGTGCCGCGGGG
CGTCGGACCGATGACGGTGACGATGCTGTTGGCCAACACCGTCCAGAGCTACAAACAGCGGCTGATCGAATAA

Upstream 100 bases:

>100_bases
ACTGCCGTGTGGGCCGACGCTTCCGGGTGATAGCATTGCCACACATTCGCAGTGGCACCCCGACGGCGATGTGAGTGCTA
CCCACAGGAGGAGTTTCCCC

Downstream 100 bases:

>100_bases
GGTCTCCAGACACTCTATTGGGCGCAGCGCCCACACAAAACTATGCCGCAGTCGACACGCGTCGTGCACGCACTGGAAGA
ATAAACCCATCCCACCCACC

Product: methylenetetrahydrofolate dehydrogenase / methenyltetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSAAILDGKALAARIQAELTCEVQSLQERFGRPPGLSVLWVGDNPASAAYVRNKNKSGQAVGIDVAKSQHLKASLSETQL
LALIDRLNADVTVDGILVQLPLPEHIDSGRVLNRIAPEKDVDGLHPVNLGRLVRGEPGLRSCTPAGVMRILSEAGVPLAG
KTAVVIGRSILVGKPVALMLLEKDATVITTHSRTADLAAITQMADVLVVAAGRPELVTAAMVRPGAVVIDVGINRVTDYE
GNSRLVGDVDFDSVRDVAAAITPVPRGVGPMTVTMLLANTVQSYKQRLIE

Sequences:

>Translated_290_residues
MSAAILDGKALAARIQAELTCEVQSLQERFGRPPGLSVLWVGDNPASAAYVRNKNKSGQAVGIDVAKSQHLKASLSETQL
LALIDRLNADVTVDGILVQLPLPEHIDSGRVLNRIAPEKDVDGLHPVNLGRLVRGEPGLRSCTPAGVMRILSEAGVPLAG
KTAVVIGRSILVGKPVALMLLEKDATVITTHSRTADLAAITQMADVLVVAAGRPELVTAAMVRPGAVVIDVGINRVTDYE
GNSRLVGDVDFDSVRDVAAAITPVPRGVGPMTVTMLLANTVQSYKQRLIE
>Mature_289_residues
SAAILDGKALAARIQAELTCEVQSLQERFGRPPGLSVLWVGDNPASAAYVRNKNKSGQAVGIDVAKSQHLKASLSETQLL
ALIDRLNADVTVDGILVQLPLPEHIDSGRVLNRIAPEKDVDGLHPVNLGRLVRGEPGLRSCTPAGVMRILSEAGVPLAGK
TAVVIGRSILVGKPVALMLLEKDATVITTHSRTADLAAITQMADVLVVAAGRPELVTAAMVRPGAVVIDVGINRVTDYEG
NSRLVGDVDFDSVRDVAAAITPVPRGVGPMTVTMLLANTVQSYKQRLIE

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=295, Percent_Identity=45.4237288135593, Blast_Score=235, Evalue=3e-62,
Organism=Homo sapiens, GI222418558, Length=297, Percent_Identity=47.4747474747475, Blast_Score=235, Evalue=3e-62,
Organism=Homo sapiens, GI94721354, Length=298, Percent_Identity=46.6442953020134, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI36796743, Length=158, Percent_Identity=30.379746835443, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1786741, Length=283, Percent_Identity=47.7031802120141, Blast_Score=248, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI17568735, Length=295, Percent_Identity=37.9661016949153, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321643, Length=303, Percent_Identity=43.2343234323432, Blast_Score=236, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6319558, Length=291, Percent_Identity=42.9553264604811, Blast_Score=233, Evalue=2e-62,
Organism=Drosophila melanogaster, GI17136816, Length=298, Percent_Identity=44.9664429530201, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI17136818, Length=298, Percent_Identity=44.9664429530201, Blast_Score=225, Evalue=2e-59,
Organism=Drosophila melanogaster, GI62472483, Length=287, Percent_Identity=46.6898954703833, Blast_Score=224, Evalue=7e-59,
Organism=Drosophila melanogaster, GI45551871, Length=287, Percent_Identity=46.6898954703833, Blast_Score=224, Evalue=7e-59,
Organism=Drosophila melanogaster, GI24645718, Length=295, Percent_Identity=46.1016949152542, Blast_Score=223, Evalue=8e-59,
Organism=Drosophila melanogaster, GI17137370, Length=295, Percent_Identity=46.1016949152542, Blast_Score=223, Evalue=8e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_GLOVI (Q7NJE8)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_924830.1
- HSSP:   P11586
- ProteinModelPortal:   Q7NJE8
- SMR:   Q7NJE8
- GeneID:   2600286
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll1884
- NMPDR:   fig|251221.1.peg.1884
- HOGENOM:   HBG328751
- OMA:   TYVRNKQ
- BioCyc:   GVIO251221:GLL1884-MONOMER
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30560; Mature: 30429

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAILDGKALAARIQAELTCEVQSLQERFGRPPGLSVLWVGDNPASAAYVRNKNKSGQA
CCCEEECCHHHHHHHHHHEEHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEECCCCCCCE
VGIDVAKSQHLKASLSETQLLALIDRLNADVTVDGILVQLPLPEHIDSGRVLNRIAPEKD
EEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCHHHHCCCCCC
VDGLHPVNLGRLVRGEPGLRSCTPAGVMRILSEAGVPLAGKTAVVIGRSILVGKPVALML
CCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCEEEECCCEEEEE
LEKDATVITTHSRTADLAAITQMADVLVVAAGRPELVTAAMVRPGAVVIDVGINRVTDYE
EECCCEEEEECCCCHHHHHHHHHHHEEEEECCCCCEEEHHHCCCCEEEEECCCCEEECCC
GNSRLVGDVDFDSVRDVAAAITPVPRGVGPMTVTMLLANTVQSYKQRLIE
CCCEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAAILDGKALAARIQAELTCEVQSLQERFGRPPGLSVLWVGDNPASAAYVRNKNKSGQA
CCEEECCHHHHHHHHHHEEHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEECCCCCCCE
VGIDVAKSQHLKASLSETQLLALIDRLNADVTVDGILVQLPLPEHIDSGRVLNRIAPEKD
EEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCHHHHCCCCCC
VDGLHPVNLGRLVRGEPGLRSCTPAGVMRILSEAGVPLAGKTAVVIGRSILVGKPVALML
CCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCEEEECCCEEEEE
LEKDATVITTHSRTADLAAITQMADVLVVAAGRPELVTAAMVRPGAVVIDVGINRVTDYE
EECCCEEEEECCCCHHHHHHHHHHHEEEEECCCCCEEEHHHCCCCEEEEECCCCEEECCC
GNSRLVGDVDFDSVRDVAAAITPVPRGVGPMTVTMLLANTVQSYKQRLIE
CCCEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292